Restriction map for pF1KE9255
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspI        AvaI        
AviII       AvrII       BanI        BbeI        BbrPI       
BclI        BcnI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrFI       BsrGI       BssHII      BstBI       BstUI       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PstI        PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflIII       3579  ACATGT               A^C[GA][CT]GT
AhdI         3118  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3218  CCTTAGG              CC^T[ACGT]AGG
Asp700I      3530  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3118  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII          50  GGACC                G^G[AT]CC
BalI         2844  TGGCCA               TGG^CCA
BamHI        1533  GGATCC               G^GATCC
BbsI         3058  GAAGAC               GAAGAC(2/6)
BcgI         1449  GCAGCTGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2791  GTATCC               GTATCC(6/5)
BglI         1246  GCCAAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        3058  GAAGAC               GAAGAC(2/6)
BseRI        2266  GAGGAG               GAGGAG(10/8)
BsmFI        1981  GGGAC                GGGAC(10/14)
BspLU11I     3579  ACATGT               A^CATGT
BsrDI        1855  GCAATG               GCAATG(2/0)
BssSI        1457  CTCGTG               CACGAG(-5/-1)
Bst1107I      881  GTATAC               GTA^TAC
BstAPI       1186  GCAACTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2622  GGTGACC              G^GT[ACGT]ACC
BstPI        2622  GGTGACC              G^GT[ACGT]ACC
BstZ17I       881  GTATAC               GTA^TAC
Bsu36I       3218  CCTTAGG              CC^T[ACGT]AGG
EaeI         2844  TGGCCA               [CT]^GGCC[GA]
Eam1105I     3118  GACTTTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3218  CCTTAGG              CC^T[ACGT]AGG
EcoO65I      2622  GGTGACC              G^GT[ACGT]ACC
FauI         2085  GCGGG                CCCGC(4/6)
HincII        412  GTTAAC               GT[CT]^[GA]AC
HindII        412  GTTAAC               GT[CT]^[GA]AC
HindIII       784  AAGCTT               A^AGCTT
HpaI          412  GTTAAC               GTT^AAC
MluNI        2844  TGGCCA               TGG^CCA
MscI         2844  TGGCCA               TGG^CCA
NdeI         1484  CATATG               CA^TATG
PshAI          51  GACCGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2482  GAGCTC               GAGCT^C
XcmI         1537  CCAGAGAAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         3530  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          881  GTATAC               GT^[AC][GT]AC
             1660  GTAGAC              
AciI         1151  CCGC                 CCGC(-3/-1)
             2085  GCGG                
AclI         1804  AACGTT               AA^CGTT
             3460  AACGTT              
AcsI         1739  AAATTT               [GA]^AATT[CT]
             3041  AAATTC              
ApoI         1739  AAATTT               [GA]^AATT[CT]
             3041  AAATTC              
AseI         2821  ATTAAT               AT^TAAT
             3288  ATTAAT              
AsnI         2821  ATTAAT               AT^TAAT
             3288  ATTAAT              
BanII        2087  GGGCTC               G[GA]GC[CT]^C
             2482  GAGCTC              
BbvCI         748  GCTGAGG              CCTCAGC(-5/-2)
             2575  GCTGAGG             
BsgI          539  CTGCAC               GTGCAG(16/14)
             1124  CTGCAC              
BspHI         291  TCATGA               T^CATGA
              834  TCATGA              
CfoI          944  GCGC                 GCG^C
             1443  GCGC                
EcoNI         159  CCTGTCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3333  CCTTGATGAGG         
EcoT22I         1  ATGCAT               ATGCA^T
             2417  ATGCAT              
HhaI          944  GCGC                 GCG^C
             1443  GCGC                
HinP1I        944  GCGC                 G^CGC
             1443  GCGC                
MslI         1135  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3420  CATTGTGGTG          
NlaIV         508  GGAACC               GG[ACGT]^[ACGT]CC
             1533  GGATCC              
NsiI            1  ATGCAT               ATGCA^T
             2417  ATGCAT              
PshBI        2821  ATTAAT               AT^TAAT
             3288  ATTAAT              
Psp1406I     1804  AACGTT               AA^CGTT
             3460  AACGTT              
RcaI          291  TCATGA               T^CATGA
              834  TCATGA              
SapI         2479  GAAGAGC              GCTCTTC(1/4)
             2983  GAAGAGC             
SphI         1077  GCATGC               GCATG^C
             1272  GCATGC              
SspI          341  AATATT               AAT^ATT
             3091  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AfaI        AluI        AlwI        AlwNI       AspHI       
BbvI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII       
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