Restriction map for pF1KE9255
 (Linear) (Six-base) MAPSORT of: KIEE9255.seq  Check: 1470  from: 1  to: 3591



KIEE9255.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     306    3591 
   Size:        306    3285
 
AccI GT'mk_AC

Cuts at:      0     882    1661    3591 
   Size:        882     779    1930

  Fragments arranged by size: 

                1930     882     779
 
AclI AA'CG_TT

Cuts at:      0    1805    3461    3591 
   Size:       1805    1656     130

  Fragments arranged by size: 

                1805    1656     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     772    1130    1325    1831    2189    2792    2890 
   Size:        746      26     358     195     506     358     603      98

Cuts at:   2890    3002    3591 
   Size:        112     589

  Fragments arranged by size: 

                 746     603     589     506     358     358     195     112
                  98      26
 
AflIII A'CryG_T

Cuts at:      0    3579    3591 
   Size:       3579      12
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3123    3591 
   Size:       3123     468
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1157    3591 
   Size:       1157    2434
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     272     304    3591 
   Size:        272      32    3287

  Fragments arranged by size: 

                3287     272      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2353    2980    3390    3591 
   Size:       2353     627     410     201

  Fragments arranged by size: 

                2353     627     410     201
 
ApoI r'AATT_y

Cuts at:      0    1739    3041    3591 
   Size:       1739    1302     550

  Fragments arranged by size: 

                1739    1302     550
 
AseI AT'TA_AT

Cuts at:      0    2822    3289    3591 
   Size:       2822     467     302

  Fragments arranged by size: 

                2822     467     302
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     722     755    3591 
   Size:        722      33    2836

  Fragments arranged by size: 

                2836     722      33
 
BamHI G'GATC_C

Cuts at:      0    1533    3591 
   Size:       1533    2058
 
BanII G_rGCy'C

Cuts at:      0    2091    2486    3591 
   Size:       2091     395    1105

  Fragments arranged by size: 

                2091    1105     395
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3065    3591 
   Size:       3065     526
 
BbvCI CC'TCA_GC

Cuts at:      0     749    2576    3591 
   Size:        749    1827    1015

  Fragments arranged by size: 

                1827    1015     749
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1438    1472    3591 
   Size:       1438      34    2119

  Fragments arranged by size: 

                2119    1438      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2802    3591 
   Size:       2802     789
 
BfrBI ATG'CAT

Cuts at:      0       3    2419    3591 
   Size:          3    2416    1172

  Fragments arranged by size: 

                2416    1172       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1252    3591 
   Size:       1252    2339
 
BlpI GC'TnA_GC

Cuts at:      0     569     744     917    3591 
   Size:        569     175     173    2674

  Fragments arranged by size: 

                2674     569     175     173
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1660    1796    3591 
   Size:       1660     136    1795

  Fragments arranged by size: 

                1795    1660     136
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     896    1655    2182    3223    3591 
   Size:        458     438     759     527    1041     368

  Fragments arranged by size: 

                1041     759     527     458     438     368
 
Bpu10I CC'TnA_GC

Cuts at:      0     749    2576    3591 
   Size:        749    1827    1015

  Fragments arranged by size: 

                1827    1015     749
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     940    2470    2597    3591 
   Size:        940    1530     127     994

  Fragments arranged by size: 

                1530     994     940     127
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1949    1979    3566    3591 
   Size:       1949      30    1587      25

  Fragments arranged by size: 

                1949    1587      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2281    3591 
   Size:       2281    1310
 
BseYI C'CCAG_C

Cuts at:      0     144    3591 
   Size:        144    3447
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1109    3591 
   Size:        524     585    2482

  Fragments arranged by size: 

                2482     585     524
 
BsiHKAI G_wGCw'C

Cuts at:      0    2001    2486    3579    3591 
   Size:       2001     485    1093      12

  Fragments arranged by size: 

                2001    1093     485      12
 
Bsp1286I G_dGCh'C

Cuts at:      0    2001    2091    2486    3579    3591 
   Size:       2001      90     395    1093      12

  Fragments arranged by size: 

                2001    1093     395      90      12
 
BspHI T'CATG_A

Cuts at:      0     291     834    3591 
   Size:        291     543    2757

  Fragments arranged by size: 

                2757     543     291
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     306    2098    3324    3591 
   Size:        306    1792    1226     267

  Fragments arranged by size: 

                1792    1226     306     267
 
BsrDI GCAATG_nn'

Cuts at:      0    1862    3591 
   Size:       1862    1729
 
BssSI C'ACGA_G

Cuts at:      0    1457    3591 
   Size:       1457    2134
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1192    3591 
   Size:       1192    2399
 
BstEII G'GTnAC_C

Cuts at:      0    2622    3591 
   Size:       2622     969
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     123    1914    3379    3483    3591 
   Size:        123    1791    1465     104     108

  Fragments arranged by size: 

                1791    1465     123     108     104
 
BstYI r'GATC_y

Cuts at:      0     384    1533    3357    3591 
   Size:        384    1149    1824     234

  Fragments arranged by size: 

                1824    1149     384     234
 
BstZ17I GTA'TAC

Cuts at:      0     883    3591 
   Size:        883    2708
 
Bsu36I CC'TnA_GG

Cuts at:      0    3219    3591 
   Size:       3219     372
 
BtsI GCAGTG_nn'

Cuts at:      0    2120    2158    3591 
   Size:       2120      38    1433

  Fragments arranged by size: 

                2120    1433      38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     299     334    3591 
   Size:        299      35    3257

  Fragments arranged by size: 

                3257     299      35
 
EaeI y'GGCC_r

Cuts at:      0    2844    3591 
   Size:       2844     747
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     394     596    2090    2165    2474    2612    2978 
   Size:         82     312     202    1494      75     309     138     366

Cuts at:   2978    3007    3313    3591 
   Size:         29     306     278

  Fragments arranged by size: 

                1494     366     312     309     306     278     202     138
                  82      75      29
 
EcoICRI GAG'CTC

Cuts at:      0    2484    3591 
   Size:       2484    1107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     746     772     896    1130    1325    1655    1831 
   Size:        458     288      26     124     234     195     330     176

Cuts at:   1831    2182    2189    2792    2890    3002    3223    3591 
   Size:        351       7     603      98     112     221     368

  Fragments arranged by size: 

                 603     458     368     351     330     288     234     221
                 195     176     124     112      98      26       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     163    3337    3591 
   Size:        163    3174     254

  Fragments arranged by size: 

                3174     254     163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     922    1207    1396    3234    3591 
   Size:        922     285     189    1838     357

  Fragments arranged by size: 

                1838     922     357     285     189
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     625     657    2485    2517    3109    3141 
   Size:        469      32     124      32    1828      32     592      32

Cuts at:   3141    3591 
   Size:        450

  Fragments arranged by size: 

                1828     592     469     450     124      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     414    3591 
   Size:        414    3177
 
HindIII A'AGCT_T

Cuts at:      0     784    3591 
   Size:        784    2807
 
HpaI GTT'AAC

Cuts at:      0     414    3591 
   Size:        414    3177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     179     637    3591 
   Size:        140      39     458    2954

  Fragments arranged by size: 

                2954     458     140      39
 
MscI TGG'CCA

Cuts at:      0    2846    3591 
   Size:       2846     745
 
MslI CAynn'nnrTG

Cuts at:      0    1139    3424    3591 
   Size:       1139    2285     167

  Fragments arranged by size: 

                2285    1139     167
 
MspA1I CmG'CkG

Cuts at:      0     505     748    1123    1153    1452    2575    3591 
   Size:        505     243     375      30     299    1123    1016

  Fragments arranged by size: 

                1123    1016     505     375     299     243      30
 
NdeI CA'TA_TG

Cuts at:      0    1485    3591 
   Size:       1485    2106
 
NsiI A_TGCA'T

Cuts at:      0       5    2421    3591 
   Size:          5    2416    1170

  Fragments arranged by size: 

                2416    1170       5
 
NspI r_CATG'y

Cuts at:      0     977    1081    1276    3583    3591 
   Size:        977     104     195    2307       8

  Fragments arranged by size: 

                2307     977     195     104       8
 
PciI A'CATG_T

Cuts at:      0    3579    3591 
   Size:       3579      12
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1935    1967    3591 
   Size:       1935      32    1624

  Fragments arranged by size: 

                1935    1624      32
 
PshAI GACnn'nnGTC

Cuts at:      0      55    3591 
   Size:         55    3536
 
PsiI TTA'TAA

Cuts at:      0     781    3591 
   Size:        781    2810
 
PvuII CAG'CTG

Cuts at:      0     505     748    1123    1452    2575    3591 
   Size:        505     243     375     329    1123    1016

  Fragments arranged by size: 

                1123    1016     505     375     329     243
 
SacI G_AGCT'C

Cuts at:      0    2486    3591 
   Size:       2486    1105
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2474    2978    3591 
   Size:       2474     504     613

  Fragments arranged by size: 

                2474     613     504
 
SfcI C'TryA_G

Cuts at:      0     551    1329    3053    3497    3591 
   Size:        551     778    1724     444      94

  Fragments arranged by size: 

                1724     778     551     444      94
 
SmlI C'TyrA_G

Cuts at:      0     955    2485    2612    3591 
   Size:        955    1530     127     979

  Fragments arranged by size: 

                1530     979     955     127
 
SphI G_CATG'C

Cuts at:      0    1081    1276    3591 
   Size:       1081     195    2315

  Fragments arranged by size: 

                2315    1081     195
 
SspI AAT'ATT

Cuts at:      0     343    3093    3591 
   Size:        343    2750     498

  Fragments arranged by size: 

                2750     498     343
 
StyI C'CwwG_G

Cuts at:      0    1823    3353    3554    3591 
   Size:       1823    1530     201      37

  Fragments arranged by size: 

                1823    1530     201      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      67    3582    3591 
   Size:         67    3515       9

  Fragments arranged by size: 

                3515      67       9
 
TatI w'GTAC_w

Cuts at:      0     378    3591 
   Size:        378    3213
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1710    1742    1949    1974    1981    2006    3566    3591 
   Size:       1710      32     207      25       7      25    1560      25

  Fragments arranged by size: 

                1710    1560     207      32      25      25      25       7
            
 
XbaI T'CTAG_A

Cuts at:      0      21    1350    2091    3591 
   Size:         21    1329     741    1500

  Fragments arranged by size: 

                1500    1329     741      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1544    3591 
   Size:       1544    2047
 
XmnI GAAnn'nnTTC

Cuts at:      0    3534    3591 
   Size:       3534      57
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     AhdI     AlfI     AloI
    AlwNI     ApoI     AseI     BaeI    BamHI    BanII     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI     BplI     BpmI   Bpu10I
    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtsI    CspCI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
     FalI    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI
   MspA1I     NdeI     NsiI     NspI     PciI     PpiI    PshAI     PsiI
    PvuII     SacI     SapI     SfcI     SmlI     SphI     SspI     StyI
    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AgeI     AleI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BanI     BbeI     BclI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BstBI     BtgI    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI    PpuMI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StuI     SwaI  Tth111I     XhoI     XmaI     ZraI
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