Restriction map for pF1KE2032
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AflII       
AgeI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvrII       BbrPI       BbsI        BcgI        BfaI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsiWI       Bsp1407I    BspDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        Eco47III    Eco81I      
EcoRI       EcoT22I     FseI        HincII      HindII      
HpaI        KpnI        KspI        MaeI        MfeI        
MluI        MunI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccIII       1145  TCCGGA               T^CCGGA
AclI         1540  AACGTT               AA^CGTT
AcsI          462  GAATTT               [GA]^AATT[CT]
AhdI         1709  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         64  GTGCAC               G^TGCAC
AlwNI        1159  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          64  GTGCAC               G^TGCAC
ApoI          462  GAATTT               [GA]^AATT[CT]
AspEI        1709  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          558  TGGCCA               TGG^CCA
BamHI        1622  GGATCC               G^GATCC
BanI          668  GGCGCC               G^G[CT][GA]CC
BbeI          668  GGCGCC               GGCGC^C
BclI         1851  TGATCA               T^GATCA
BglI          208  GCCTTCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1337  AGATCT               A^GATCT
BsaWI        1145  TCCGGA               [AT]^CCGG[AT]
BseAI        1145  TCCGGA               T^CCGGA
BsmI          538  GAATGC               GAATGC(1/-1)
BspEI        1145  TCCGGA               T^CCGGA
BspHI        1718  TCATGA               T^CATGA
BsrBI        1594  GAGCGG               CCGCTC(-3/-3)
BsrDI        1386  CATTGC               GCAATG(2/0)
BsrFI        1599  GCCGGT               [GA]^CCGG[CT]
BstEII        678  GGTGACC              G^GT[ACGT]ACC
BstPI         678  GGTGACC              G^GT[ACGT]ACC
Cfr10I       1599  GCCGGT               [GA]^CCGG[CT]
DraIII       1435  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1192  GACCGCTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1597  CGGCCG               C^GGCCG
Eam1105I     1709  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1597  CGGCCG               C^GGCCG
Eco52I       1597  CGGCCG               C^GGCCG
EcoNI        1663  CCTGATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       678  GGTGACC              G^GT[ACGT]ACC
EcoRV        1320  GATATC               GAT^ATC
FbaI         1851  TGATCA               T^GATCA
HaeII         668  GGCGCC               [GA]GCGC^[CT]
HgaI          282  GACGC                GACGC(5/10)
HindIII       295  AAGCTT               A^AGCTT
KasI          668  GGCGCC               G^GCGCC
MluNI         558  TGGCCA               TGG^CCA
MroI         1145  TCCGGA               T^CCGGA
MscI          558  TGGCCA               TGG^CCA
MseI         1733  TTAA                 T^TAA
MslI         1809  CACACATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          668  GGCGCC               GG^CGCC
PaeR7I        287  CTCGAG               C^TCGAG
Psp1406I     1540  AACGTT               AA^CGTT
PstI         1567  CTGCAG               CTGCA^G
RcaI         1718  TCATGA               T^CATGA
SacI         1060  GAGCTC               GAGCT^C
SapI         1722  GAAGAGC              GCTCTTC(1/4)
SbfI         1566  CCTGCAGG             CCTGCA^GG
SexAI        1294  ACCAGGT              A^CC[AT]GGT
SfoI          668  GGCGCC               GGC^GCC
SmlI          287  CTCGAG               C^T[CT][GA]AG
Sse8387I     1566  CCTGCAGG             CCTGCA^GG
StuI          206  AGGCCT               AGG^CCT
Tru9I        1733  TTAA                 T^TAA
Tsp509I       463  AATT                 ^AATT
XcmI          131  CCAACACCTTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          287  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          282  GACGCC               G[GA]^CG[CT]C
              668  GGCGCC              
AflIII       1640  ACATGT               A^C[GA][CT]GT
             1812  ACATGT              
AspHI          64  GTGCAC               G[AT]GC[AT]^C
             1060  GAGCTC              
AvaI          287  CTCGAG               C^[CT]CG[GA]G
              990  CTCGGG              
AviII         152  TGCGCA               TGC^GCA
             1271  TGCGCA              
BbvCI          91  CCTCAGC              CCTCAGC(-5/-2)
              325  GCTGAGG             
BciVI        1486  GGATAC               GTATCC(6/5)
             1835  GGATAC              
BsaBI        1338  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1623  GATCCGCATC          
BsaHI         282  GACGCC               G[GA]^CG[CT]C
              668  GGCGCC              
BsgI          192  GTGCAG               GTGCAG(16/14)
              763  GTGCAG              
BsiEI         103  CGGTCG               CG[GA][CT]^CG
             1597  CGGCCG              
BsiHKAI        64  GTGCAC               G[AT]GC[AT]^C
             1060  GAGCTC              
BsoBI         287  CTCGAG               C^[CT]CG[GA]G
              990  CTCGGG              
BspLU11I     1640  ACATGT               A^CATGT
             1812  ACATGT              
BstAPI        377  GCATTGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1131  GCAGGTGATGC         
BstYI        1337  AGATCT               [GA]^GATC[CT]
             1622  GGATCC              
EarI         1149  GAAGAG               CTCTTC(1/4)
             1722  GAAGAG              
EcoT14I       725  CCAAGG               C^C[AT][AT]GG
             1126  CCTTGG              
FspI          152  TGCGCA               TGC^GCA
             1271  TGCGCA              
Hin1I         282  GACGCC               G[GA]^CG[CT]C
              668  GGCGCC              
Ksp632I      1149  GAAGAG               CTCTTC(1/4)
             1722  GAAGAG              
MamI         1338  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1623  GATCCGCATC          
MflI         1337  AGATCT               [GA]^GATC[CT]
             1622  GGATCC              
StyI          725  CCAAGG               C^C[AT][AT]GG
             1126  CCTTGG              
TfiI          612  GAATC                G^A[AT]TC
             1069  GAATC               
XhoII        1337  AGATCT               [GA]^GATC[CT]
             1622  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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