Restriction map for pF1KE6673
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MseI        
MspA1I      MunI        MvnI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcsI          456  GAATTT               [GA]^AATT[CT]
AlwI          110  GGATC                GGATC(4/5)
ApaI           24  GGGCCC               GGGCC^C
ApoI          456  GAATTT               [GA]^AATT[CT]
BalI          483  TGGCCA               TGG^CCA
BbeI          760  GGCGCC               GGCGC^C
BbsI          781  GTCTTC               GAAGAC(2/6)
BcgI          104  GCACCTGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          253  CTAG                 C^TAG
BpmI          622  CTCCAG               CTGGAG(16/14)
BpuAI         781  GTCTTC               GAAGAC(2/6)
BsgI          358  GTGCAG               GTGCAG(16/14)
BsiEI          44  CGGCCG               CG[GA][CT]^CG
BsiWI          32  CGTACG               C^GTACG
BsmAI         366  GTCTC                GTCTC(1/5)
BspHI         188  TCATGA               T^CATGA
BssSI         555  CACGAG               CACGAG(-5/-1)
BstF5I        165  GGATG                GGATG(2/0)
BstXI         647  CCAGCCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          398  CTAAG                C^T[ACGT]AG
DrdI          421  GACATTGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           44  CGGCCG               C^GGCCG
EarI          368  CTCTTC               CTCTTC(1/4)
EclXI          44  CGGCCG               C^GGCCG
Eco52I         44  CGGCCG               C^GGCCG
EcoNI         312  CCTGTTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          165  GGATG                GGATG(9/13)
HaeII         760  GGCGCC               [GA]GCGC^[CT]
HinfI         119  GACTC                G^A[ACGT]TC
HphI          122  TCACC                GGTGA(8/7)
KasI          760  GGCGCC               G^GCGCC
Ksp632I       368  CTCTTC               CTCTTC(1/4)
MaeI          253  CTAG                 C^TAG
MaeIII        719  GTGAC                ^GT[ACGT]AC
MluNI         483  TGGCCA               TGG^CCA
MscI          483  TGGCCA               TGG^CCA
NarI          760  GGCGCC               GG^CGCC
PleI          119  GACTC                GAGTC(4/5)
RcaI          188  TCATGA               T^CATGA
SfoI          760  GGCGCC               GGC^GCC
SgrAI         858  CACCGGCG             C[GA]^CCGG[CT]G
Tsp45I        719  GTGAC                ^GT[GC]AC
Tsp509I       457  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          427  GGCGTC               G[GA]^CG[CT]C
              760  GGCGCC              
AspHI           3  GAGCTC               G[AT]GC[AT]^C
               97  GAGCTC              
BbvI          405  GCTGC                GCAGC(8/12)
              501  GCTGC               
BsaHI         427  GGCGTC               G[GA]^CG[CT]C
              760  GGCGCC              
BseRI         726  CTCCTC               GAGGAG(10/8)
              913  GAGGAG              
BsiHKAI         3  GAGCTC               G[AT]GC[AT]^C
               97  GAGCTC              
BspMI         354  GCAGGT               ACCTGC(4/8)
              360  GCAGGT              
CfoI          524  GCGC                 GCG^C
              761  GCGC                
DpnII         111  GATC                 ^GATC
              222  GATC                
DsaI          281  CCATGG               C^C[GA][CT]GG
              599  CCATGG              
EcoT14I       281  CCATGG               C^C[AT][AT]GG
              599  CCATGG              
FauI          822  CCCGC                CCCGC(4/6)
              413  GCGGG               
HhaI          524  GCGC                 GCG^C
              761  GCGC                
Hin1I         427  GGCGTC               G[GA]^CG[CT]C
              760  GGCGCC              
HinP1I        524  GCGC                 G^CGC
              761  GCGC                
MboI          111  GATC                 ^GATC
              222  GATC                
MboII         369  TCTTC                GAAGA(8/7)
              782  TCTTC               
NaeI          794  GCCGGC               GCC^GGC
              919  GCCGGC              
NcoI          281  CCATGG               C^CATGG
              599  CCATGG              
NdeII         111  GATC                 ^GATC
              222  GATC                
NgoMIV        794  GCCGGC               G^CCGGC
              919  GCCGGC              
PpuMI         673  GGGACCC              [GA]G^G[AT]CC[CT]
              884  AGGTCCC             
SacI            3  GAGCTC               GAGCT^C
               97  GAGCTC              
Sau3AI        111  GATC                 ^GATC
              222  GATC                
ScaI          434  AGTACT               AGT^ACT
              815  AGTACT              
SfaNI         466  GATGC                GCATC(5/9)
              564  GCATC               
SmaI          670  CCCGGG               CCC^GGG
              876  CCCGGG              
StyI          281  CCATGG               C^C[AT][AT]GG
              599  CCATGG              
TseI          405  GCTGC                G^C[AT]GC
              501  GCTGC               
TspRI         487  CAGTG                CA[GC]TG[ACGT][ACGT]^
              833  CAGTG               
XmaCI         670  CCCGGG               C^CCGGG
              876  CCCGGG              
XmaI          670  CCCGGG               C^CCGGG
              876  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BanI        BanII       BcnI        BglI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BstNI       Cac8I       Cfr10I      Cfr13I      DraII       
EaeI        EcoO109I    EcoRII      Fnu4HI      HaeIII      
HapII       HgaI        HpaII       ItaI        MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PstI        RsaI        Sau96I      
ScrFI       SfcI        TaqI        TthHB8I     
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