################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 18 GACGTC GACGT^C AccII 124 CGCG CG^CG AfaI 384 GTAC GT^AC AocI 354 CCTCAGG CC^T[ACGT]AGG AspI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 423 GGACC G^G[AT]CC AviII 608 TGCGCA TGC^GCA BanI 388 GGCGCC G^G[CT][GA]CC BanII 340 GAGCCC G[GA]GC[CT]^C BbeI 388 GGCGCC GGCGC^C BbsI 364 GTCTTC GAAGAC(2/6) BglII 488 AGATCT A^GATCT BmyI 340 GAGCCC G[AGT]GC[ACT]^C BpmI 337 CTGGAG CTGGAG(16/14) BpuAI 364 GTCTTC GAAGAC(2/6) BsmAI 598 GAGAC GTCTC(1/5) BsmFI 21 GTCCC GGGAC(10/14) Bsp1286I 340 GAGCCC G[AGT]GC[ACT]^C BsrBI 624 CCGCTC CCGCTC(-3/-3) BstUI 124 CGCG CG^CG BstXI 371 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 488 AGATCT [GA]^GATC[CT] Bsu36I 354 CCTCAGG CC^T[ACGT]AGG Cfr13I 423 GGACC G^G[ACGT]CC DpnII 489 GATC ^GATC DraII 422 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 397 GACAGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 354 CCTCAGG CC^T[ACGT]AGG EcoO109I 422 AGGACCT [GA]G^G[ACGT]CC[CT] FspI 608 TGCGCA TGC^GCA HaeII 388 GGCGCC [GA]GCGC^[CT] HgaI 538 GACGC GACGC(5/10) HinfI 244 GAGTC G^A[ACGT]TC KasI 388 GGCGCC G^GCGCC MboI 489 GATC ^GATC MflI 488 AGATCT [GA]^GATC[CT] MslI 529 CACAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 124 CGCG CG^CG NarI 388 GGCGCC GG^CGCC NdeII 489 GATC ^GATC NlaIII 585 CATG CATG^ PflFI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 244 GAGTC GAGTC(4/5) PpuMI 422 AGGACCT [GA]G^G[AT]CC[CT] PstI 211 CTGCAG CTGCA^G RsaI 384 GTAC GT^AC Sau3AI 489 GATC ^GATC Sau96I 423 GGACC G^G[ACGT]CC SfoI 388 GGCGCC GGC^GCC SmlI 547 CTCAAG C^T[CT][GA]AG Tth111I 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 488 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 18 GACGTC G[GA]^CG[CT]C 388 GGCGCC BcnI 14 CCCGG CC^[GC]GG 476 CCCGG BfaI 295 CTAG C^TAG 427 CTAG BsaHI 18 GACGTC G[GA]^CG[CT]C 388 GGCGCC BsaJI 55 CCCTGG C^C[ACGT][ACGT]GG 475 CCCCGG BsrI 481 ACTGG ACTGG(1/-1) 327 CCAGT BstF5I 324 CATCC GGATG(2/0) 438 CATCC DdeI 240 CTCAG C^T[ACGT]AG 355 CTCAG FokI 324 CATCC GGATG(9/13) 438 CATCC HaeIII 40 GGCC GG^CC 147 GGCC HapII 15 CCGG C^CGG 477 CCGG Hin1I 18 GACGTC G[GA]^CG[CT]C 388 GGCGCC HpaII 15 CCGG C^CGG 477 CCGG MaeI 295 CTAG C^TAG 427 CTAG MaeII 19 ACGT A^CGT 404 ACGT MspA1I 142 CAGCTG C[AC]G^C[GT]G 639 CAGCTG MspI 15 CCGG C^CGG 477 CCGG NciI 14 CCCGG CC^[GC]GG 476 CCCGG PvuII 142 CAGCTG CAG^CTG 639 CAGCTG TaqI 515 TCGA T^CGA 596 TCGA TspRI 72 CAGTG CA[GC]TG[ACGT][ACGT]^ 619 CAGTG TthHB8I 515 TCGA T^CGA 596 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AluI BbvI BsgI BsiYI BslI BssKI BstNI Cac8I CfoI EcoNI EcoRII Fnu4HI HhaI HinP1I ItaI MboII MnlI MvaI MwoI NlaIV ScrFI SfaNI SfcI TseI