Restriction map for
pF1KE0054
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MluI MroI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TthHB8I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 2695 GTAGAC GT^[AC][GT]AC
AccII 69 CGCG CG^CG
AlwNI 1418 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 340 CCTCAGG CC^T[ACGT]AGG
ApaI 2762 GGGCCC GGGCC^C
AspI 2976 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2966 CCCGAG C^[CT]CG[GA]G
BanI 2524 GGCACC G^G[CT][GA]CC
BcnI 73 CCCGG CC^[GC]GG
BglII 2799 AGATCT A^GATCT
BsaI 490 GAGACC GGTCTC(1/5)
BsaWI 2035 TCCGGT [AT]^CCGG[AT]
BsgI 2225 CTGCAC GTGCAG(16/14)
BsmBI 2782 CGTCTC CGTCTC(1/5)
BsmI 2736 GAATGC GAATGC(1/-1)
BsoBI 2966 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2649 TGTACA T^GTACA
BsrDI 869 CATTGC GCAATG(2/0)
BsrGI 2649 TGTACA T^GTACA
BstAPI 535 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 69 CGCG CG^CG
BstXI 2287 CCAGAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 340 CCTCAGG CC^T[ACGT]AGG
CfoI 70 GCGC GCG^C
DraIII 2228 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2744 CCATGG C^C[GA][CT]GG
Eco81I 340 CCTCAGG CC^T[ACGT]AGG
EcoNI 2122 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 937 ATGCAT ATGCA^T
HgaI 2236 GACGC GACGC(5/10)
HhaI 70 GCGC GCG^C
HinP1I 70 GCGC G^CGC
MfeI 2984 CAATTG C^AATTG
MunI 2984 CAATTG C^AATTG
MvnI 69 CGCG CG^CG
NciI 73 CCCGG CC^[GC]GG
NcoI 2744 CCATGG C^CATGG
NdeI 2309 CATATG CA^TATG
NsiI 937 ATGCAT ATGCA^T
PflFI 2976 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1898 AGGACCT [GA]G^G[AT]CC[CT]
PstI 123 CTGCAG CTGCA^G
SspBI 2649 TGTACA T^GTACA
Tth111I 2976 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 315 TCTAGA T^CTAGA
XcmI 1340 CCACAGTGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AvaII 347 GGACC G^G[AT]CC
1899 GGACC
BalI 1337 TGGCCA TGG^CCA
2296 TGGCCA
BclI 2534 TGATCA T^GATCA
2946 TGATCA
EcoT14I 2127 CCAAGG C^C[AT][AT]GG
2744 CCATGG
FauI 103 CCCGC CCCGC(4/6)
83 GCGGG
FbaI 2534 TGATCA T^GATCA
2946 TGATCA
HapII 74 CCGG C^CGG
2036 CCGG
HincII 2217 GTCAAC GT[CT]^[GA]AC
2806 GTTGAC
HindII 2217 GTCAAC GT[CT]^[GA]AC
2806 GTTGAC
HindIII 820 AAGCTT A^AGCTT
2717 AAGCTT
HpaII 74 CCGG C^CGG
2036 CCGG
MluNI 1337 TGGCCA TGG^CCA
2296 TGGCCA
MscI 1337 TGGCCA TGG^CCA
2296 TGGCCA
MspI 74 CCGG C^CGG
2036 CCGG
NspI 1684 GCATGT [GA]CATG^[CT]
2439 GCATGT
SacI 150 GAGCTC GAGCT^C
1395 GAGCTC
ScaI 590 AGTACT AGT^ACT
2259 AGTACT
StyI 2127 CCAAGG C^C[AT][AT]GG
2744 CCATGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII