Restriction map for
pF1KE0054
(Linear) (Six-base) MAPSORT of: KIEE0054.seq Check: 6126 from: 1 to: 3039
KIEE0054.orf
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AccI GT'mk_AC
Cuts at: 0 2696 3039
Size: 2696 343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 356 2273 2284 2604 3039
Size: 161 195 1917 11 320 435
Fragments arranged by size:
1917 435 320 195 161 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1199 1415 3039
Size: 1199 216 1624
Fragments arranged by size:
1624 1199 216
AlwNI CAG_nnn'CTG
Cuts at: 0 1423 3039
Size: 1423 1616
ApaI G_GGCC'C
Cuts at: 0 2766 3039
Size: 2766 273
ApaLI G'TGCA_C
Cuts at: 0 772 2473 2749 3039
Size: 772 1701 276 290
Fragments arranged by size:
1701 772 290 276
ApoI r'AATT_y
Cuts at: 0 721 1061 1218 1231 1496 1813 3039
Size: 721 340 157 13 265 317 1226
Fragments arranged by size:
1226 721 340 317 265 157 13
AvaI C'yCGr_G
Cuts at: 0 2966 3039
Size: 2966 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1694 1727 2123 2156 3039
Size: 1694 33 396 33 883
Fragments arranged by size:
1694 883 396 33 33
BanI G'GyrC_C
Cuts at: 0 2524 3039
Size: 2524 515
BanII G_rGCy'C
Cuts at: 0 91 154 1399 2766 3039
Size: 91 63 1245 1367 273
Fragments arranged by size:
1367 1245 273 91 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 803 2512 2820 3039
Size: 803 1709 308 219
Fragments arranged by size:
1709 803 308 219
BclI T'GATC_A
Cuts at: 0 2534 2946 3039
Size: 2534 412 93
Fragments arranged by size:
2534 412 93
BfrBI ATG'CAT
Cuts at: 0 939 3039
Size: 939 2100
BglII A'GATC_T
Cuts at: 0 2799 3039
Size: 2799 240
Bme1580I G_kGCm'C
Cuts at: 0 776 2477 2753 2766 3039
Size: 776 1701 276 13 273
Fragments arranged by size:
1701 776 276 273 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 1565 2519 3039
Size: 1565 954 520
Fragments arranged by size:
1565 954 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1352 2120 2270 2511 3039
Size: 1156 196 768 150 241 528
Fragments arranged by size:
1156 768 528 241 196 150
Bpu10I CC'TnA_GC
Cuts at: 0 2413 3039
Size: 2413 626
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 3039
Size: 484 2555
BsaWI w'CCGG_w
Cuts at: 0 2035 3039
Size: 2035 1004
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 638 668 1773 1803 1989 2019 2268 2298
Size: 638 30 1105 30 186 30 249 30
Cuts at: 2298 3039
Size: 741
Fragments arranged by size:
1105 741 638 249 186 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 120 1994 3039
Size: 30 90 1874 1045
Fragments arranged by size:
1874 1045 90 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2210 3039
Size: 2210 829
BsiHKAI G_wGCw'C
Cuts at: 0 154 748 776 1129 1399 2477 2753 3039
Size: 154 594 28 353 270 1078 276 286
Fragments arranged by size:
1078 594 353 286 276 270 154 28
BsmI GAATG_Cn'
Cuts at: 0 2742 3039
Size: 2742 297
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2788 3039
Size: 2788 251
Bsp1286I G_dGCh'C
Cuts at: 0 91 154 748 776 1129 1399 2477 2753
Size: 91 63 594 28 353 270 1078 276
Cuts at: 2753 2766 3039
Size: 13 273
Fragments arranged by size:
1078 594 353 276 273 270 91 63
28 13
BsrDI GCAATG_nn'
Cuts at: 0 868 3039
Size: 868 2171
BsrGI T'GTAC_A
Cuts at: 0 2649 3039
Size: 2649 390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 541 3039
Size: 541 2498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2294 3039
Size: 2294 745
BstYI r'GATC_y
Cuts at: 0 309 969 1297 2341 2624 2799 2996 3039
Size: 309 660 328 1044 283 175 197 43
Fragments arranged by size:
1044 660 328 309 283 197 175 43
Bsu36I CC'TnA_GG
Cuts at: 0 341 3039
Size: 341 2698
BtgI C'CryG_G
Cuts at: 0 2744 3039
Size: 2744 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2946 3039
Size: 2946 93
DraI TTT'AAA
Cuts at: 0 726 1965 1995 3039
Size: 726 1239 30 1044
Fragments arranged by size:
1239 1044 726 30
DraIII CAC_nnn'GTG
Cuts at: 0 2233 3039
Size: 2233 806
EaeI y'GGCC_r
Cuts at: 0 17 1337 2296 3039
Size: 17 1320 959 743
Fragments arranged by size:
1320 959 743 17
EarI CTCTTCn'nnn_
Cuts at: 0 112 375 467 905 2603 3039
Size: 112 263 92 438 1698 436
Fragments arranged by size:
1698 438 436 263 112 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3039
Size: 19 3020
EcoICRI GAG'CTC
Cuts at: 0 152 1397 3039
Size: 152 1245 1642
Fragments arranged by size:
1642 1245 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 356 1156 1352 2120 2270 2273 2284
Size: 161 195 800 196 768 150 3 11
Cuts at: 2284 2511 2604 3039
Size: 227 93 435
Fragments arranged by size:
800 768 435 227 196 195 161 150
93 11 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2126 3039
Size: 2126 913
EcoO109I rG'GnC_Cy
Cuts at: 0 1899 2762 2763 3039
Size: 1899 863 1 276
Fragments arranged by size:
1899 863 276 1
EcoRI G'AATT_C
Cuts at: 0 1218 1231 1813 3039
Size: 1218 13 582 1226
Fragments arranged by size:
1226 1218 582 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1976 3039
Size: 1976 1063
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 772 804 954 986 1873 1905
Size: 157 32 583 32 150 32 887 32
Cuts at: 1905 3039
Size: 1134
Fragments arranged by size:
1134 887 583 157 150 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2219 2808 3039
Size: 2219 589 231
Fragments arranged by size:
2219 589 231
HindIII A'AGCT_T
Cuts at: 0 820 2717 3039
Size: 820 1897 322
Fragments arranged by size:
1897 820 322
MfeI C'AATT_G
Cuts at: 0 2984 3039
Size: 2984 55
MscI TGG'CCA
Cuts at: 0 1339 2298 3039
Size: 1339 959 741
Fragments arranged by size:
1339 959 741
MslI CAynn'nnrTG
Cuts at: 0 896 1880 2027 2572 3039
Size: 896 984 147 545 467
Fragments arranged by size:
984 896 545 467 147
NcoI C'CATG_G
Cuts at: 0 2744 3039
Size: 2744 295
NdeI CA'TA_TG
Cuts at: 0 2310 3039
Size: 2310 729
NsiI A_TGCA'T
Cuts at: 0 941 3039
Size: 941 2098
NspI r_CATG'y
Cuts at: 0 1688 2443 3039
Size: 1688 755 596
Fragments arranged by size:
1688 755 596
PasI CC'CwG_GG
Cuts at: 0 108 3039
Size: 108 2931
PpuMI rG'GwC_Cy
Cuts at: 0 1899 3039
Size: 1899 1140
PspOMI G'GGCC_C
Cuts at: 0 2762 3039
Size: 2762 277
PstI C_TGCA'G
Cuts at: 0 127 3039
Size: 127 2912
SacI G_AGCT'C
Cuts at: 0 154 1399 3039
Size: 154 1245 1640
Fragments arranged by size:
1640 1245 154
SapI GCTCTTCn'nnn_
Cuts at: 0 112 467 2603 3039
Size: 112 355 2136 436
Fragments arranged by size:
2136 436 355 112
ScaI AGT'ACT
Cuts at: 0 592 2261 3039
Size: 592 1669 778
Fragments arranged by size:
1669 778 592
SfcI C'TryA_G
Cuts at: 0 123 1028 2009 3039
Size: 123 905 981 1030
Fragments arranged by size:
1030 981 905 123
StyI C'CwwG_G
Cuts at: 0 2127 2744 3039
Size: 2127 617 295
Fragments arranged by size:
2127 617 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 3039
Size: 1012 2027
TatI w'GTAC_w
Cuts at: 0 590 2259 2649 2930 3039
Size: 590 1669 390 281 109
Fragments arranged by size:
1669 590 390 281 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2268 2300 3039
Size: 2268 32 739
Fragments arranged by size:
2268 739 32
Tth111I GACn'n_nGTC
Cuts at: 0 2979 3039
Size: 2979 60
XbaI T'CTAG_A
Cuts at: 0 315 3039
Size: 315 2724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1347 3039
Size: 1347 1692
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BbsI BclI BfrBI BglII Bme1580I
BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII MfeI MscI MslI NcoI NdeI NsiI
NspI PasI PpuMI PspOMI PstI SacI SapI ScaI
SfcI StyI TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BciVI BglI BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstZ17I BtsI ClaI CspCI DrdI EagI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MmeI MspA1I NaeI NarI NgoMIV NheI NotI NruI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
XhoI XmaI XmnI ZraI