(Circular) (Six-base) MAPSORT of: pF1KE2430.seq Check: 4237 from: 1 to: 4712 pF1KE2430 4712 bp With 182 enzymes: SgfI * January 7, 2013 14:38 .. AatII G_ACGT'C Cuts at: 287 287 Size: 4712 AccI GT'mk_AC Cuts at: 1750 1750 Size: 4712 Acc65I G'GTAC_C Cuts at: 1728 1728 Size: 4712 AclI AA'CG_TT Cuts at: 4552 4552 Size: 4712 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 826 1138 1571 2537 2969 3716 3984 826 Size: 312 433 966 432 747 268 1554 Fragments arranged by size: 1554 966 747 433 432 312 268 AflIII A'CryG_T Cuts at: 1232 2064 3168 4145 1232 Size: 832 1104 977 1799 Fragments arranged by size: 1799 1104 977 832 AgeI A'CCGG_T Cuts at: 3100 3100 Size: 4712 AleI CACnn'nnGTG Cuts at: 1687 1687 Size: 4712 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 920 920 Size: 4712 AlwNI CAG_nnn'CTG Cuts at: 375 2017 3584 375 Size: 1642 1567 1503 Fragments arranged by size: 1642 1567 1503 ApaI G_GGCC'C Cuts at: 1699 1699 Size: 4712 ApaLI G'TGCA_C Cuts at: 378 3482 378 Size: 3104 1608 ApoI r'AATT_y Cuts at: 1716 4065 4321 1716 Size: 2349 256 2107 Fragments arranged by size: 2349 2107 256 AseI AT'TA_AT Cuts at: 20 655 20 Size: 635 4077 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4712 AvaI C'yCGr_G Cuts at: 137 216 1732 137 Size: 79 1516 3117 Fragments arranged by size: 3117 1516 79 AvrII C'CTAG_G Cuts at: 270 270 Size: 4712 BamHI G'GATC_C Cuts at: 1737 1737 Size: 4712 BanI G'GyrC_C Cuts at: 204 220 545 1728 2142 2390 2425 204 Size: 16 325 1183 414 248 35 2491 Fragments arranged by size: 2491 1183 414 325 248 35 16 BanII G_rGCy'C Cuts at: 217 839 974 1148 1699 1726 2756 217 Size: 622 135 174 551 27 1030 2173 Fragments arranged by size: 2173 1030 622 551 174 135 27 BbeI G_GCGC'C Cuts at: 208 224 2146 2394 208 Size: 16 1922 248 2526 Fragments arranged by size: 2526 1922 248 16 BbsI GAAGACnn'nnnn_ Cuts at: 243 243 Size: 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 694 728 694 Size: 34 4678 BciVI GTATCCnnnnn_n' Cuts at: 2610 3371 4712 2610 Size: 761 1341 2610 Fragments arranged by size: 2610 1341 761 BclI T'GATC_A Cuts at: 2232 2232 Size: 4712 BglII A'GATC_T Cuts at: 2227 4264 2227 Size: 2037 2675 BlpI GC'TnA_GC Cuts at: 1824 1824 Size: 4712 Bme1580I G_kGCm'C Cuts at: 382 1699 2337 2430 3486 382 Size: 1317 638 93 1056 1608 Fragments arranged by size: 1608 1317 1056 638 93 BmgBI CAC'GTC Cuts at: 1233 1233 Size: 4712 BmrI ACTGGGnnnn_n' Cuts at: 1694 1924 2005 2340 4484 1694 Size: 230 81 335 2144 1922 Fragments arranged by size: 2144 1922 335 230 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1512 3079 1512 Size: 1567 3145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 805 1325 805 Size: 520 4192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1888 2767 3259 3557 1888 Size: 879 492 298 3043 Fragments arranged by size: 3043 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4416 4416 Size: 4712 BsaAI yAC'GTr Cuts at: 2065 2065 Size: 4712 BsaBI GATnn'nnATC Cuts at: 6 1538 2226 4263 6 Size: 1532 688 2037 455 Fragments arranged by size: 2037 1532 688 455 BsaHI Gr'CG_yC Cuts at: 205 221 284 1082 2143 2391 3093 4589 205 Size: 16 63 798 1061 248 702 1496 328 Fragments arranged by size: 1496 1061 798 702 328 248 63 16 BsaWI w'CCGG_w Cuts at: 560 1901 2422 3100 3374 3521 3932 560 Size: 1341 521 678 274 147 411 1340 Fragments arranged by size: 1341 1340 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 154 1417 154 Size: 1263 3449 BseYI C'CCAG_C Cuts at: 463 497 2137 2895 3472 3968 463 Size: 34 1640 758 577 496 1207 Fragments arranged by size: 1640 1207 758 577 496 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1637 1637 Size: 4712 BsiEI CG_ry'CG Cuts at: 104 238 338 2254 2300 3508 104 Size: 134 100 1916 46 1208 1308 Fragments arranged by size: 1916 1308 1208 134 100 46 BsiHKAI G_wGCw'C Cuts at: 350 382 687 1148 1726 2504 2694 3486 350 Size: 32 305 461 578 778 190 792 1576 Fragments arranged by size: 1576 792 778 578 461 305 190 32 BsmI GAATG_Cn' Cuts at: 574 574 Size: 4712 BsmBI CGTCTCn'nnnn_ Cuts at: 1475 1475 Size: 4712 Bsp1286I G_dGCh'C Cuts at: 217 350 382 687 839 974 1148 1699 1726 Size: 133 32 305 152 135 174 551 27 Cuts at: 1726 2337 2430 2504 2694 2756 3486 217 Size: 611 93 74 190 62 730 1443 Fragments arranged by size: 1443 730 611 551 305 190 174 152 135 133 93 74 62 32 27 BspEI T'CCGG_A Cuts at: 3932 3932 Size: 4712 BspHI T'CATG_A Cuts at: 1178 3888 1178 Size: 2710 2002 BspMI ACCTGCnnnn'nnnn_ Cuts at: 505 1762 1976 2278 2659 505 Size: 1257 214 302 381 2558 Fragments arranged by size: 2558 1257 381 302 214 BsrBI CCG'CTC Cuts at: 50 3004 3058 4542 4707 50 Size: 2954 54 1484 165 55 Fragments arranged by size: 2954 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2624 2624 Size: 4712 BsrFI r'CCGG_y Cuts at: 233 337 2710 2891 3100 233 Size: 104 2373 181 209 1845 Fragments arranged by size: 2373 1845 209 181 104 BsrGI T'GTAC_A Cuts at: 649 649 Size: 4712 BssSI C'ACGA_G Cuts at: 2983 3341 2983 Size: 358 4354 BstAPI GCAn_nnn'nTGC Cuts at: 2089 2089 Size: 4712 BstBI TT'CG_AA Cuts at: 3073 3073 Size: 4712 BstYI r'GATC_y Cuts at: 1 419 1737 2204 2227 2562 2808 3820 3906 Size: 418 1318 467 23 335 246 1012 86 Cuts at: 3906 3918 4264 1 Size: 12 346 449 Fragments arranged by size: 1318 1012 467 449 418 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 851 851 Size: 4712 BtgI C'CryG_G Cuts at: 107 294 107 Size: 187 4525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2662 2843 3006 2662 Size: 181 163 4368 Fragments arranged by size: 4368 181 163 BtsI GCAGTG_nn' Cuts at: 947 972 1955 947 Size: 25 983 3704 Fragments arranged by size: 3704 983 25 DraI TTT'AAA Cuts at: 959 1711 959 Size: 752 3960 DraIII CAC_nnn'GTG Cuts at: 682 682 Size: 4712 DrdI GACnn_nn'nnGTC Cuts at: 2418 3276 2418 Size: 858 3854 EaeI y'GGCC_r Cuts at: 235 335 408 1187 2297 2471 2862 2889 4234 Size: 100 73 779 1110 174 391 27 1345 Cuts at: 4234 235 Size: 713 Fragments arranged by size: 1345 1110 779 713 391 174 100 73 27 EagI C'GGCC_G Cuts at: 235 335 2297 235 Size: 100 1962 2650 Fragments arranged by size: 2650 1962 100 EarI CTCTTCn'nnn_ Cuts at: 1317 1362 1367 2735 2945 1317 Size: 45 5 1368 210 3084 Fragments arranged by size: 3084 1368 210 45 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2920 3230 3376 4522 2920 Size: 310 146 1146 3110 Fragments arranged by size: 3110 1146 310 146 EcoICRI GAG'CTC Cuts at: 1146 1724 1146 Size: 578 4134 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 805 826 1138 1325 1571 2537 2969 3716 3984 Size: 21 312 187 246 966 432 747 268 Cuts at: 3984 805 Size: 1533 Fragments arranged by size: 1533 966 747 432 312 268 246 187 21 EcoO109I rG'GnC_Cy Cuts at: 142 846 1545 1659 1695 1696 1851 142 Size: 704 699 114 36 1 155 3003 Fragments arranged by size: 3003 704 699 155 114 36 1 EcoRI G'AATT_C Cuts at: 1716 1716 Size: 4712 EcoRV GAT'ATC Cuts at: 610 610 Size: 4712 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 589 2731 589 Size: 2142 2570 FspI TGC'GCA Cuts at: 2493 2493 Size: 4712 HaeII r_GCGC'y Cuts at: 208 224 2146 2394 3416 4395 208 Size: 16 1922 248 1022 979 525 Fragments arranged by size: 1922 1022 979 525 248 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2554 2584 2586 2616 2554 Size: 30 2 30 4650 Fragments arranged by size: 4650 30 30 2 HincII GTy'rAC Cuts at: 1751 1751 Size: 4712 KasI G'GCGC_C Cuts at: 204 220 2142 2390 204 Size: 16 1922 248 2526 Fragments arranged by size: 2526 1922 248 16 KpnI G_GTAC'C Cuts at: 1732 1732 Size: 4712 MluI A'CGCG_T Cuts at: 4145 4145 Size: 4712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 516 601 3383 3567 516 Size: 85 2782 184 1661 Fragments arranged by size: 2782 1661 184 85 MscI TGG'CCA Cuts at: 1189 2473 1189 Size: 1284 3428 MslI CAynn'nnrTG Cuts at: 902 1202 1687 2701 2828 902 Size: 300 485 1014 127 2786 Fragments arranged by size: 2786 1014 485 300 127 MspA1I CmG'CkG Cuts at: 183 375 399 467 1667 1823 2137 2497 3510 Size: 192 24 68 1200 156 314 360 1013 Cuts at: 3510 3755 183 Size: 245 1140 Fragments arranged by size: 1200 1140 1013 360 314 245 192 156 68 24 NaeI GCC'GGC Cuts at: 235 339 2893 235 Size: 104 2554 2054 Fragments arranged by size: 2554 2054 104 NarI GG'CG_CC Cuts at: 205 221 2143 2391 205 Size: 16 1922 248 2526 Fragments arranged by size: 2526 1922 248 16 NcoI C'CATG_G Cuts at: 107 107 Size: 4712 NdeI CA'TA_TG Cuts at: 530 870 530 Size: 340 4372 NgoMIV G'CCGG_C Cuts at: 233 337 2891 233 Size: 104 2554 2054 Fragments arranged by size: 2554 2054 104 NspI r_CATG'y Cuts at: 1765 2265 3172 1765 Size: 500 907 3305 Fragments arranged by size: 3305 907 500 PciI A'CATG_T Cuts at: 3168 3168 Size: 4712 PflMI CCAn_nnn'nTGG Cuts at: 1447 1447 Size: 4712 PfoI T'CCnGG_A Cuts at: 350 350 Size: 4712 PmeI GTTT'AAAC Cuts at: 1711 1711 Size: 4712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 3851 Size: 32 4680 PpuMI rG'GwC_Cy Cuts at: 846 1545 1659 846 Size: 699 114 3899 Fragments arranged by size: 3899 699 114 PspOMI G'GGCC_C Cuts at: 1695 1695 Size: 4712 PstI C_TGCA'G Cuts at: 954 1759 2444 954 Size: 805 685 3222 Fragments arranged by size: 3222 805 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4712 PvuII CAG'CTG Cuts at: 399 467 2137 2497 399 Size: 68 1670 360 2614 Fragments arranged by size: 2614 1670 360 68 RsrII CG'GwC_CG Cuts at: 2907 2907 Size: 4712 SacI G_AGCT'C Cuts at: 1148 1726 1148 Size: 578 4134 SalI G'TCGA_C Cuts at: 1749 1749 Size: 4712 SanDI GG'GwC_CC Cuts at: 1545 1545 Size: 4712 SapI GCTCTTCn'nnn_ Cuts at: 2735 2945 2735 Size: 210 4502 SbfI CC_TGCA'GG Cuts at: 1759 1759 Size: 4712 ScaI AGT'ACT Cuts at: 4229 4229 Size: 4712 SexAI A'CCwGG_T Cuts at: 245 245 Size: 4712 SfcI C'TryA_G Cuts at: 33 764 950 1001 1612 1755 2440 3433 3624 Size: 731 186 51 611 143 685 993 191 Cuts at: 3624 33 Size: 1121 Fragments arranged by size: 1121 993 731 685 611 191 186 143 51 SfoI GGC'GCC Cuts at: 206 222 2144 2392 206 Size: 16 1922 248 2526 Fragments arranged by size: 2526 1922 248 16 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4712 SmaI CCC'GGG Cuts at: 1734 1734 Size: 4712 SmlI C'TyrA_G Cuts at: 1867 2782 3274 3536 1867 Size: 915 492 262 3043 Fragments arranged by size: 3043 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4712 SphI G_CATG'C Cuts at: 1765 2265 1765 Size: 500 4212 SspI AAT'ATT Cuts at: 1290 1372 1290 Size: 82 4630 StyI C'CwwG_G Cuts at: 107 270 1676 1699 1846 2200 107 Size: 163 1406 23 147 354 2619 Fragments arranged by size: 2619 1406 354 163 147 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2295 2428 2887 3096 2295 Size: 133 459 209 3911 Fragments arranged by size: 3911 459 209 133 TatI w'GTAC_w Cuts at: 649 1643 4227 649 Size: 994 2584 1134 Fragments arranged by size: 2584 1134 994 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4229 4261 4229 Size: 32 4680 Tth111I GACn'n_nGTC Cuts at: 2509 2509 Size: 4712 XbaI T'CTAG_A Cuts at: 1743 1743 Size: 4712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 403 1332 2207 403 Size: 929 875 2908 Fragments arranged by size: 2908 929 875 XmaI C'CCGG_G Cuts at: 1732 1732 Size: 4712 XmnI GAAnn'nnTTC Cuts at: 572 572 Size: 4712 ZraI GAC'GTC Cuts at: 285 285 Size: 4712 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AloI AscI BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI BsiWI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI StuI SwaI XhoI