Restriction map for pF1KE2479
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AscI        Asp718I     AspEI       
AspI        BamHI       BbrPI       BbvCI       BcgI        
BfrI        BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrGI       BssSI       
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
DraIII      Eam1105I    EcoRI       EcoT22I     FseI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NcoI        NdeI        NheI        NruI        
NsiI        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         2374  GTAGAC               GT^[AC][GT]AC
AflIII       2317  ACACGT               A^C[GA][CT]GT
Alw44I        526  GTGCAC               G^TGCAC
AocI         1020  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       122  AGCGCT               AGC^GCT
ApaI           69  GGGCCC               GGGCC^C
ApaLI         526  GTGCAC               G^TGCAC
AseI         2008  ATTAAT               AT^TAAT
AsnI         2008  ATTAAT               AT^TAAT
AvaI          636  CTCGAG               C^[CT]CG[GA]G
AvrII        1381  CCTAGG               C^CTAGG
BbeI           53  GGCGCC               GGCGC^C
BbsI         2458  GAAGAC               GAAGAC(2/6)
BciVI        1939  GGATAC               GTATCC(6/5)
BclI         1914  TGATCA               T^GATCA
BlnI         1381  CCTAGG               C^CTAGG
BpuAI        2458  GAAGAC               GAAGAC(2/6)
BsaAI         340  TACGTG               [CT]AC^GT[GA]
BsiEI         562  CGGCCG               CG[GA][CT]^CG
BsmFI         590  GGGAC                GGGAC(10/14)
BsmI         2300  GCATTC               GAATGC(1/-1)
BsoBI         636  CTCGAG               C^[CT]CG[GA]G
BsrBI         559  GAGCGG               CCGCTC(-3/-3)
BsrFI          50  GCCGGC               [GA]^CCGG[CT]
BssHII        298  GCGCGC               G^CGCGC
BstBI        1222  TTCGAA               TT^CGAA
BstEII        264  GGTGACC              G^GT[ACGT]ACC
BstPI         264  GGTGACC              G^GT[ACGT]ACC
BstXI         488  CCATCATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1020  CCTAAGG              CC^T[ACGT]AGG
Cfr10I         50  GCCGGC               [GA]^CCGG[CT]
DrdI         1504  GACAGTTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           93  CCGCGG               C^C[GA][CT]GG
EagI          562  CGGCCG               C^GGCCG
EclXI         562  CGGCCG               C^GGCCG
Eco47III      122  AGCGCT               AGC^GCT
Eco52I        562  CGGCCG               C^GGCCG
Eco81I       1020  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1211  CCTCAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       264  GGTGACC              G^GT[ACGT]ACC
EcoRV        2428  GATATC               GAT^ATC
FbaI         1914  TGATCA               T^GATCA
HindIII       908  AAGCTT               A^AGCTT
HpaI         2170  GTTAAC               GTT^AAC
KasI           53  GGCGCC               G^GCGCC
KspI           93  CCGCGG               CCGC^GG
NaeI           50  GCCGGC               GCC^GGC
NarI           53  GGCGCC               GG^CGCC
NgoMIV         50  GCCGGC               G^CCGGC
NotI          561  GCGGCCGC             GC^GGCCGC
NspV         1222  TTCGAA               TT^CGAA
PaeR7I        636  CTCGAG               C^TCGAG
PshBI        2008  ATTAAT               AT^TAAT
PvuII         235  CAGCTG               CAG^CTG
SacII          93  CCGCGG               CCGC^GG
SfiI          735  GGCCAAGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           53  GGCGCC               GGC^GCC
SfuI         1222  TTCGAA               TT^CGAA
SphI         2528  GCATGC               GCATG^C
XhoI          636  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI         1130  AAATTC               [GA]^AATT[CT]
             1921  AAATTC              
AlwNI         418  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1253  CAGTTACTG           
ApoI         1130  AAATTC               [GA]^AATT[CT]
             1921  AAATTC              
Asp700I       756  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              850  GAAGCCATTC          
AviII         164  TGCGCA               TGC^GCA
              344  TGCGCA              
BglI          664  GCCAGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              736  GCCAAGCTGGC         
BlpI          720  GCTCAGC              GC^T[ACGT]AGC
             1641  GCTCAGC             
Bpu1102I      720  GCTCAGC              GC^T[ACGT]AGC
             1641  GCTCAGC             
BsmBI         394  CGTCTC               CGTCTC(1/5)
               21  GAGACG              
BsrDI        1440  CATTGC               GCAATG(2/0)
              792  GCAATG              
BstAPI        417  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              528  GCACATGCTGC         
CelII         720  GCTCAGC              GC^T[ACGT]AGC
             1641  GCTCAGC             
EarI         2251  CTCTTC               CTCTTC(1/4)
              841  GAAGAG              
EcoT14I      1381  CCTAGG               C^C[AT][AT]GG
             1806  CCTTGG              
FauI           39  GCGGG                CCCGC(4/6)
              587  GCGGG               
FspI          164  TGCGCA               TGC^GCA
              344  TGCGCA              
HaeII          53  GGCGCC               [GA]GCGC^[CT]
              122  AGCGCT              
HincII        199  GTCAAC               GT[CT]^[GA]AC
             2170  GTTAAC              
HindII        199  GTCAAC               GT[CT]^[GA]AC
             2170  GTTAAC              
Ksp632I      2251  CTCTTC               CTCTTC(1/4)
              841  GAAGAG              
MslI           27  CATGACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              153  CATCCCAGTG          
MspA1I         93  CCGCGG               C[AC]G^C[GT]G
              235  CAGCTG              
PpuMI         695  AGGACCC              [GA]G^G[AT]CC[CT]
             1377  AGGACCT             
SacI          718  GAGCTC               GAGCT^C
             1363  GAGCTC              
SspI         1872  AATATT               AAT^ATT
             1933  AATATT              
StuI         1017  AGGCCT               AGG^CCT
             1617  AGGCCT              
StyI         1381  CCTAGG               C^C[AT][AT]GG
             1806  CCTTGG              
XmnI          756  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              850  GAAGCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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