Restriction map for pF1KE2479
 (Linear) (Six-base) MAPSORT of: KIEE2479.seq  Check: 5519  from: 1  to: 2544



KIEE2479.orf

 With 182 enzymes: SgfI * 

                        August 3, 2016 15:28  ..

AccI GT'mk_AC

Cuts at:      0    2375    2544 
   Size:       2375     169
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     606    1263    1459    1634    1907    2185    2544 
   Size:        606     657     196     175     273     278     359

  Fragments arranged by size: 

                 657     606     359     278     273     196     175
 
AfeI AGC'GCT

Cuts at:      0     124    2544 
   Size:        124    2420
 
AflIII A'CryG_T

Cuts at:      0    2317    2544 
   Size:       2317     227
 
AlwNI CAG_nnn'CTG

Cuts at:      0     423    1258    2544 
   Size:        423     835    1286

  Fragments arranged by size: 

                1286     835     423
 
ApaI G_GGCC'C

Cuts at:      0      73    2544 
   Size:         73    2471
 
ApaLI G'TGCA_C

Cuts at:      0     526    2544 
   Size:        526    2018
 
ApoI r'AATT_y

Cuts at:      0    1130    1921    2544 
   Size:       1130     791     623

  Fragments arranged by size: 

                1130     791     623
 
AseI AT'TA_AT

Cuts at:      0    2009    2544 
   Size:       2009     535
 
AvaI C'yCGr_G

Cuts at:      0     636    2544 
   Size:        636    1908
 
AvrII C'CTAG_G

Cuts at:      0    1381    2544 
   Size:       1381    1163
 
BanI G'GyrC_C

Cuts at:      0      53      97     547    2544 
   Size:         53      44     450    1997

  Fragments arranged by size: 

                1997     450      53      44
 
BanII G_rGCy'C

Cuts at:      0      73     409     722    1367    2544 
   Size:         73     336     313     645    1177

  Fragments arranged by size: 

                1177     645     336     313      73
 
BbeI G_GCGC'C

Cuts at:      0      57    2544 
   Size:         57    2487
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2465    2544 
   Size:       2465      79
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1933    2544 
   Size:       1933     611
 
BclI T'GATC_A

Cuts at:      0    1914    2544 
   Size:       1914     630
 
BglI GCCn_nnn'nGGC

Cuts at:      0     670     742    2544 
   Size:        670      72    1802

  Fragments arranged by size: 

                1802     670      72
 
BglII A'GATC_T

Cuts at:      0     867    1274    1700    2544 
   Size:        867     407     426     844

  Fragments arranged by size: 

                 867     844     426     407
 
BlpI GC'TnA_GC

Cuts at:      0     721    1642    2544 
   Size:        721     921     902

  Fragments arranged by size: 

                 921     902     721
 
Bme1580I G_kGCm'C

Cuts at:      0      73     530    2544 
   Size:         73     457    2014

  Fragments arranged by size: 

                2014     457      73
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     151    1290    2544 
   Size:        151    1139    1254

  Fragments arranged by size: 

                1254    1139     151
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1029    2544 
   Size:       1029    1515
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     280     910    2418    2544 
   Size:        196      84     630    1508     126

  Fragments arranged by size: 

                1508     630     196     126      84
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265    2253    2544 
   Size:        265    1988     291

  Fragments arranged by size: 

                1988     291     265
 
BsaAI yAC'GTr

Cuts at:      0     342    2544 
   Size:        342    2202
 
BsaHI Gr'CG_yC

Cuts at:      0      54     134     311    2544 
   Size:         54      80     177    2233

  Fragments arranged by size: 

                2233     177      80      54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2094    2124    2544 
   Size:       2094      30     420

  Fragments arranged by size: 

                2094     420      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     133     193    2544 
   Size:        130       3      60    2351

  Fragments arranged by size: 

                2351     130      60       3
 
BseYI C'CCAG_C

Cuts at:      0    1122    2524    2544 
   Size:       1122    1402      20

  Fragments arranged by size: 

                1402    1122      20
 
BsiEI CG_ry'CG

Cuts at:      0     565    2544 
   Size:        565    1979
 
BsiHKAI G_wGCw'C

Cuts at:      0     257     530     722    1367    1643    1907    2544 
   Size:        257     273     192     645     276     264     637

  Fragments arranged by size: 

                 645     637     276     273     264     257     192
 
BsmI GAATG_Cn'

Cuts at:      0    2300    2544 
   Size:       2300     244
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      15     400    2544 
   Size:         15     385    2144

  Fragments arranged by size: 

                2144     385      15
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     257     409     530     722    1367    1643    1907 
   Size:         73     184     152     121     192     645     276     264

Cuts at:   1907    2544 
   Size:        637

  Fragments arranged by size: 

                 645     637     276     264     192     184     152     121
                  73
 
BsrBI CCG'CTC

Cuts at:      0     561    2544 
   Size:        561    1983
 
BsrDI GCAATG_nn'

Cuts at:      0     799    1439    2544 
   Size:        799     640    1105

  Fragments arranged by size: 

                1105     799     640
 
BsrFI r'CCGG_y

Cuts at:      0      50    2544 
   Size:         50    2494
 
BssHII G'CGCG_C

Cuts at:      0     298    2544 
   Size:        298    2246
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     423     534    2544 
   Size:        423     111    2010

  Fragments arranged by size: 

                2010     423     111
 
BstBI TT'CG_AA

Cuts at:      0    1223    2544 
   Size:       1223    1321
 
BstEII G'GTnAC_C

Cuts at:      0     264    2544 
   Size:        264    2280
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495    2544 
   Size:        495    2049
 
BstYI r'GATC_y

Cuts at:      0     822     867    1274    1700    2544 
   Size:        822      45     407     426     844

  Fragments arranged by size: 

                 844     822     426     407      45
 
Bsu36I CC'TnA_GG

Cuts at:      0    1021    2544 
   Size:       1021    1523
 
BtgI C'CryG_G

Cuts at:      0      93    2544 
   Size:         93    2451
 
BtsI GCAGTG_nn'

Cuts at:      0     504    2544 
   Size:        504    2040
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1510    2544 
   Size:       1510    1034
 
EaeI y'GGCC_r

Cuts at:      0     383     562     662     734     743    1088    2544 
   Size:        383     179     100      72       9     345    1456

  Fragments arranged by size: 

                1456     383     345     179     100      72       9
 
EagI C'GGCC_G

Cuts at:      0     562    2544 
   Size:        562    1982
 
EarI CTCTTCn'nnn_

Cuts at:      0     836    2257    2544 
   Size:        836    1421     287

  Fragments arranged by size: 

                1421     836     287
 
EcoICRI GAG'CTC

Cuts at:      0     720    1365    2544 
   Size:        720     645    1179

  Fragments arranged by size: 

                1179     720     645
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     280     606     910    1263    1459    1634    1907 
   Size:        196      84     326     304     353     196     175     273

Cuts at:   1907    2185    2418    2544 
   Size:        278     233     126

  Fragments arranged by size: 

                 353     326     304     278     273     233     196     196
                 175     126      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1215    2544 
   Size:       1215    1329
 
EcoO109I rG'GnC_Cy

Cuts at:      0      69     696    1378    2544 
   Size:         69     627     682    1166

  Fragments arranged by size: 

                1166     682     627      69
 
EcoRV GAT'ATC

Cuts at:      0    2430    2544 
   Size:       2430     114
 
FspI TGC'GCA

Cuts at:      0     166     346    2544 
   Size:        166     180    2198

  Fragments arranged by size: 

                2198     180     166
 
FspAI rTGC'GCAy

Cuts at:      0     346    2544 
   Size:        346    2198
 
HaeII r_GCGC'y

Cuts at:      0      57     126    2544 
   Size:         57      69    2418

  Fragments arranged by size: 

                2418      69      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     859     891    2544 
   Size:        859      32    1653

  Fragments arranged by size: 

                1653     859      32
 
HincII GTy'rAC

Cuts at:      0     201    2172    2544 
   Size:        201    1971     372

  Fragments arranged by size: 

                1971     372     201
 
HindIII A'AGCT_T

Cuts at:      0     908    2544 
   Size:        908    1636
 
HpaI GTT'AAC

Cuts at:      0    2172    2544 
   Size:       2172     372
 
KasI G'GCGC_C

Cuts at:      0      53    2544 
   Size:         53    2491
 
MscI TGG'CCA

Cuts at:      0     664     736     745    1090    2544 
   Size:        664      72       9     345    1454

  Fragments arranged by size: 

                1454     664     345      72       9
 
MslI CAynn'nnrTG

Cuts at:      0      31     157    2544 
   Size:         31     126    2387

  Fragments arranged by size: 

                2387     126      31
 
MspA1I CmG'CkG

Cuts at:      0      95     237    2544 
   Size:         95     142    2307

  Fragments arranged by size: 

                2307     142      95
 
NaeI GCC'GGC

Cuts at:      0      52    2544 
   Size:         52    2492
 
NarI GG'CG_CC

Cuts at:      0      54    2544 
   Size:         54    2490
 
NgoMIV G'CCGG_C

Cuts at:      0      50    2544 
   Size:         50    2494
 
NotI GC'GGCC_GC

Cuts at:      0     562    2544 
   Size:        562    1982
 
NspI r_CATG'y

Cuts at:      0     534    1168    1785    1995    2532    2544 
   Size:        534     634     617     210     537      12

  Fragments arranged by size: 

                 634     617     537     534     210      12
 
PasI CC'CwG_GG

Cuts at:      0    1071    2544 
   Size:       1071    1473
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     745     777    2544 
   Size:        745      32    1767

  Fragments arranged by size: 

                1767     745      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     696    1378    2544 
   Size:        696     682    1166

  Fragments arranged by size: 

                1166     696     682
 
PsiI TTA'TAA

Cuts at:      0    2347    2544 
   Size:       2347     197
 
PspOMI G'GGCC_C

Cuts at:      0      69    2544 
   Size:         69    2475
 
PspXI vC'TCGA_Gb

Cuts at:      0     636    2544 
   Size:        636    1908
 
PstI C_TGCA'G

Cuts at:      0     236     428    1700    2544 
   Size:        236     192    1272     844

  Fragments arranged by size: 

                1272     844     236     192
 
PvuII CAG'CTG

Cuts at:      0     237    2544 
   Size:        237    2307
 
SacI G_AGCT'C

Cuts at:      0     722    1367    2544 
   Size:        722     645    1177

  Fragments arranged by size: 

                1177     722     645
 
SacII CC_GC'GG

Cuts at:      0      96    2544 
   Size:         96    2448
 
SfcI C'TryA_G

Cuts at:      0     232     424    1696    1838    2544 
   Size:        232     192    1272     142     706

  Fragments arranged by size: 

                1272     706     232     192     142
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     742    2544 
   Size:        742    1802
 
SfoI GGC'GCC

Cuts at:      0      55    2544 
   Size:         55    2489
 
SmlI C'TyrA_G

Cuts at:      0     280     636    2268    2544 
   Size:        280     356    1632     276

  Fragments arranged by size: 

                1632     356     280     276
 
SphI G_CATG'C

Cuts at:      0    2532    2544 
   Size:       2532      12
 
SspI AAT'ATT

Cuts at:      0    1874    1935    2544 
   Size:       1874      61     609

  Fragments arranged by size: 

                1874     609      61
 
StuI AGG'CCT

Cuts at:      0    1019    1619    2544 
   Size:       1019     600     925

  Fragments arranged by size: 

                1019     925     600
 
StyI C'CwwG_G

Cuts at:      0    1381    1806    2544 
   Size:       1381     425     738

  Fragments arranged by size: 

                1381     738     425
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     283     802    1309    2544 
   Size:        283     519     507    1235

  Fragments arranged by size: 

                1235     519     507     283
 
XhoI C'TCGA_G

Cuts at:      0     636    2544 
   Size:        636    1908
 
XmnI GAAnn'nnTTC

Cuts at:      0     760     854    2544 
   Size:        760      94    1690

  Fragments arranged by size: 

                1690     760      94
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI    ApaLI     ApoI
     AseI     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI
     PasI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PstI    PvuII
     SacI    SacII     SfcI     SfiI     SfoI     SmlI     SphI     SspI
     StuI     StyI    TaqII     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     AscI    AsiSI     BaeI    BamHI    BbvCI     BcgI
    BfrBI    BmgBI     BmtI   Bpu10I     BsaI    BsaBI    BsaWI     BsgI
    BsiWI    BspEI    BspHI    BspMI    BsrGI    BssSI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     EciI    EcoRI     FalI     FseI
     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
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