(Linear) (Six-base) MAPSORT of: KIEE3386.seq Check: 405 from: 1 to: 3621 KIEE3386.orf With 182 enzymes: SgfI * June 25, 2015 11:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2359 3621 Size: 2359 1262 AclI AA'CG_TT Cuts at: 0 1761 3621 Size: 1761 1860 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 509 1292 1418 1618 2197 3070 3621 Size: 107 402 783 126 200 579 873 551 Fragments arranged by size: 873 783 579 551 402 200 126 107 AflIII A'CryG_T Cuts at: 0 1303 2335 2560 3621 Size: 1303 1032 225 1061 Fragments arranged by size: 1303 1061 1032 225 AhdI GACnn_n'nnGTC Cuts at: 0 1002 2415 3621 Size: 1002 1413 1206 Fragments arranged by size: 1413 1206 1002 AleI CACnn'nnGTG Cuts at: 0 1011 2765 3621 Size: 1011 1754 856 Fragments arranged by size: 1754 1011 856 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 917 2247 3621 Size: 917 1330 1374 Fragments arranged by size: 1374 1330 917 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1796 1828 3621 Size: 1796 32 1793 Fragments arranged by size: 1796 1793 32 AlwNI CAG_nnn'CTG Cuts at: 0 3183 3343 3621 Size: 3183 160 278 Fragments arranged by size: 3183 278 160 ApaI G_GGCC'C Cuts at: 0 3162 3588 3621 Size: 3162 426 33 Fragments arranged by size: 3162 426 33 ApaLI G'TGCA_C Cuts at: 0 2758 3621 Size: 2758 863 ApoI r'AATT_y Cuts at: 0 40 111 3621 Size: 40 71 3510 Fragments arranged by size: 3510 71 40 AseI AT'TA_AT Cuts at: 0 1856 3621 Size: 1856 1765 BamHI G'GATC_C Cuts at: 0 1126 3621 Size: 1126 2495 BanII G_rGCy'C Cuts at: 0 3162 3404 3466 3588 3621 Size: 3162 242 62 122 33 Fragments arranged by size: 3162 242 122 62 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 515 805 2037 3621 Size: 515 290 1232 1584 Fragments arranged by size: 1584 1232 515 290 BciVI GTATCCnnnnn_n' Cuts at: 0 1024 1380 3459 3621 Size: 1024 356 2079 162 Fragments arranged by size: 2079 1024 356 162 BclI T'GATC_A Cuts at: 0 230 3621 Size: 230 3391 BfrBI ATG'CAT Cuts at: 0 1488 2998 3621 Size: 1488 1510 623 Fragments arranged by size: 1510 1488 623 BglI GCCn_nnn'nGGC Cuts at: 0 3074 3621 Size: 3074 547 BglII A'GATC_T Cuts at: 0 555 3621 Size: 555 3066 BlpI GC'TnA_GC Cuts at: 0 1519 3621 Size: 1519 2102 Bme1580I G_kGCm'C Cuts at: 0 1147 2644 2762 3162 3424 3588 3621 Size: 1147 1497 118 400 262 164 33 Fragments arranged by size: 1497 1147 400 262 164 118 33 BmrI ACTGGGnnnn_n' Cuts at: 0 1360 1925 2629 2761 3399 3621 Size: 1360 565 704 132 638 222 Fragments arranged by size: 1360 704 638 565 222 132 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 574 1806 2176 3621 Size: 574 1232 370 1445 Fragments arranged by size: 1445 1232 574 370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 985 3621 Size: 96 889 2636 Fragments arranged by size: 2636 889 96 Bpu10I CC'TnA_GC Cuts at: 0 938 3145 3621 Size: 938 2207 476 Fragments arranged by size: 2207 938 476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1306 1454 3304 3621 Size: 1306 148 1850 317 Fragments arranged by size: 1850 1306 317 148 BsaI GGTCTCn'nnnn_ Cuts at: 0 1040 3038 3621 Size: 1040 1998 583 Fragments arranged by size: 1998 1040 583 BsaAI yAC'GTr Cuts at: 0 1142 3621 Size: 1142 2479 BsaBI GATnn'nnATC Cuts at: 0 2354 3621 Size: 2354 1267 BsaHI Gr'CG_yC Cuts at: 0 2826 3621 Size: 2826 795 BsaWI w'CCGG_w Cuts at: 0 1049 3621 Size: 1049 2572 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 695 725 1649 1679 3391 3421 3621 Size: 695 30 924 30 1712 30 200 Fragments arranged by size: 1712 924 695 200 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2643 3130 3229 3249 3621 Size: 2643 487 99 20 372 Fragments arranged by size: 2643 487 372 99 20 BseYI C'CCAG_C Cuts at: 0 1631 2584 3621 Size: 1631 953 1037 Fragments arranged by size: 1631 1037 953 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 1043 1122 1448 2769 3621 Size: 116 927 79 326 1321 852 Fragments arranged by size: 1321 927 852 326 116 79 BsiHKAI G_wGCw'C Cuts at: 0 2508 2698 2762 3621 Size: 2508 190 64 859 Fragments arranged by size: 2508 859 190 64 BsmI GAATG_Cn' Cuts at: 0 1225 2781 3621 Size: 1225 1556 840 Fragments arranged by size: 1556 1225 840 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2164 3621 Size: 2164 1457 Bsp1286I G_dGCh'C Cuts at: 0 1147 2508 2644 2698 2762 3162 3404 3424 Size: 1147 1361 136 54 64 400 242 20 Cuts at: 3424 3466 3588 3621 Size: 42 122 33 Fragments arranged by size: 1361 1147 400 242 136 122 64 54 42 33 20 BspHI T'CATG_A Cuts at: 0 1554 3621 Size: 1554 2067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2359 2818 3621 Size: 2359 459 803 Fragments arranged by size: 2359 803 459 BsrDI GCAATG_nn' Cuts at: 0 758 1215 3621 Size: 758 457 2406 Fragments arranged by size: 2406 758 457 BsrFI r'CCGG_y Cuts at: 0 2690 3621 Size: 2690 931 BssSI C'ACGA_G Cuts at: 0 1799 3621 Size: 1799 1822 BstEII G'GTnAC_C Cuts at: 0 766 2053 3621 Size: 766 1287 1568 Fragments arranged by size: 1568 1287 766 BstXI CCAn_nnnn'nTGG Cuts at: 0 1373 1891 2716 3389 3412 3621 Size: 1373 518 825 673 23 209 Fragments arranged by size: 1373 825 673 518 209 23 BstYI r'GATC_y Cuts at: 0 555 1126 2226 3621 Size: 555 571 1100 1395 Fragments arranged by size: 1395 1100 571 555 Bsu36I CC'TnA_GG Cuts at: 0 2878 3621 Size: 2878 743 BtgI C'CryG_G Cuts at: 0 153 3078 3621 Size: 153 2925 543 Fragments arranged by size: 2925 543 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3045 3621 Size: 3045 576 BtsI GCAGTG_nn' Cuts at: 0 2078 3621 Size: 2078 1543 DraI TTT'AAA Cuts at: 0 470 1751 3621 Size: 470 1281 1870 Fragments arranged by size: 1870 1281 470 DraIII CAC_nnn'GTG Cuts at: 0 877 986 3621 Size: 877 109 2635 Fragments arranged by size: 2635 877 109 DrdI GACnn_nn'nnGTC Cuts at: 0 2430 3621 Size: 2430 1191 EaeI y'GGCC_r Cuts at: 0 1695 1823 1893 2003 3075 3354 3621 Size: 1695 128 70 110 1072 279 267 Fragments arranged by size: 1695 1072 279 267 128 110 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 107 509 985 1292 1418 1618 2197 Size: 96 11 402 476 307 126 200 579 Cuts at: 2197 3070 3621 Size: 873 551 Fragments arranged by size: 873 579 551 476 402 307 200 126 96 11 EcoO109I rG'GnC_Cy Cuts at: 0 127 1252 3158 3584 3585 3621 Size: 127 1125 1906 426 1 36 Fragments arranged by size: 1906 1125 426 127 36 1 EcoRI G'AATT_C Cuts at: 0 111 3621 Size: 111 3510 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1596 3621 Size: 1596 2025 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 695 727 988 1020 2401 2433 2712 2744 Size: 695 32 261 32 1381 32 279 32 Cuts at: 2744 3253 3285 3448 3480 3621 Size: 509 32 163 32 141 Fragments arranged by size: 1381 695 509 279 261 163 141 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 3334 3621 Size: 3334 287 HindIII A'AGCT_T Cuts at: 0 3489 3621 Size: 3489 132 MfeI C'AATT_G Cuts at: 0 202 3621 Size: 202 3419 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 566 754 3621 Size: 566 188 2867 Fragments arranged by size: 2867 566 188 MscI TGG'CCA Cuts at: 0 1825 2005 3077 3356 3621 Size: 1825 180 1072 279 265 Fragments arranged by size: 1825 1072 279 265 180 MslI CAynn'nnrTG Cuts at: 0 248 1011 1232 1493 2467 2565 2765 2960 Size: 248 763 221 261 974 98 200 195 Cuts at: 2960 3103 3621 Size: 143 518 Fragments arranged by size: 974 763 518 261 248 221 200 195 143 98 MspA1I CmG'CkG Cuts at: 0 610 2361 2765 2783 3621 Size: 610 1751 404 18 838 Fragments arranged by size: 1751 838 610 404 18 NcoI C'CATG_G Cuts at: 0 153 3621 Size: 153 3468 NdeI CA'TA_TG Cuts at: 0 3497 3621 Size: 3497 124 NsiI A_TGCA'T Cuts at: 0 1490 3000 3621 Size: 1490 1510 621 Fragments arranged by size: 1510 1490 621 NspI r_CATG'y Cuts at: 0 1020 1307 1488 2175 2466 2526 2564 2817 Size: 1020 287 181 687 291 60 38 253 Cuts at: 2817 2973 3002 3621 Size: 156 29 619 Fragments arranged by size: 1020 687 619 291 287 253 181 156 60 38 29 PciI A'CATG_T Cuts at: 0 1303 2560 3621 Size: 1303 1257 1061 Fragments arranged by size: 1303 1257 1061 PflMI CCAn_nnn'nTGG Cuts at: 0 1530 3343 3480 3621 Size: 1530 1813 137 141 Fragments arranged by size: 1813 1530 141 137 PmlI CAC'GTG Cuts at: 0 1142 3621 Size: 1142 2479 PpuMI rG'GwC_Cy Cuts at: 0 127 1252 3621 Size: 127 1125 2369 Fragments arranged by size: 2369 1125 127 PshAI GACnn'nnGTC Cuts at: 0 2829 3621 Size: 2829 792 PsiI TTA'TAA Cuts at: 0 248 3301 3621 Size: 248 3053 320 Fragments arranged by size: 3053 320 248 PspOMI G'GGCC_C Cuts at: 0 3158 3584 3621 Size: 3158 426 37 Fragments arranged by size: 3158 426 37 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1142 1174 1299 1331 3621 Size: 1142 32 125 32 2290 Fragments arranged by size: 2290 1142 125 32 32 PstI C_TGCA'G Cuts at: 0 976 3621 Size: 976 2645 PvuII CAG'CTG Cuts at: 0 610 2361 2765 2783 3621 Size: 610 1751 404 18 838 Fragments arranged by size: 1751 838 610 404 18 ScaI AGT'ACT Cuts at: 0 1057 3621 Size: 1057 2564 SfcI C'TryA_G Cuts at: 0 972 1148 2066 3168 3621 Size: 972 176 918 1102 453 Fragments arranged by size: 1102 972 918 453 176 SmlI C'TyrA_G Cuts at: 0 1285 1469 3319 3621 Size: 1285 184 1850 302 Fragments arranged by size: 1850 1285 302 184 SphI G_CATG'C Cuts at: 0 2175 3002 3621 Size: 2175 827 619 Fragments arranged by size: 2175 827 619 StuI AGG'CCT Cuts at: 0 1889 3246 3621 Size: 1889 1357 375 Fragments arranged by size: 1889 1357 375 StyI C'CwwG_G Cuts at: 0 153 911 1884 1890 2163 2709 3286 3621 Size: 153 758 973 6 273 546 577 335 Fragments arranged by size: 973 758 577 546 335 273 153 6 SwaI ATTT'AAAT Cuts at: 0 1751 3621 Size: 1751 1870 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 258 1097 2072 2617 3621 Size: 258 839 975 545 1004 Fragments arranged by size: 1004 975 839 545 258 TatI w'GTAC_w Cuts at: 0 653 1055 1099 1967 3621 Size: 653 402 44 868 1654 Fragments arranged by size: 1654 868 653 402 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1044 1076 1649 1681 2074 2106 2884 2916 Size: 1044 32 573 32 393 32 778 32 Cuts at: 2916 3621 Size: 705 Fragments arranged by size: 1044 778 705 573 393 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3364 3621 Size: 3364 257 XmnI GAAnn'nnTTC Cuts at: 0 444 672 3621 Size: 444 228 2949 Fragments arranged by size: 2949 444 228 Enzymes that do cut: AarI AclI AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI BamHI BanII BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PmlI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII ScaI SfcI SmlI SphI StuI StyI SwaI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AgeI AscI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BmgBI BmtI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I ClaI CspCI EagI EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PpiI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI Tth111I XbaI XhoI XmaI ZraI