Restriction map for
pF1KB8518
(Circular) (Six-base) MAPSORT of: pF1KB8518.seq Check: 5894 from: 1 to: 5720
pF1KB8518 5720 bp
With 182 enzymes: SgfI *
October 12, 2011 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2220 2220
Size: 5720
AatII G_ACGT'C
Cuts at: 1510 1510
Size: 5720
AccI GT'mk_AC
Cuts at: 2758 2758
Size: 5720
Acc65I G'GTAC_C
Cuts at: 2736 2736
Size: 5720
AclI AA'CG_TT
Cuts at: 5560 5560
Size: 5720
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 3545 3977 4724 4992 144
Size: 3401 432 747 268 872
Fragments arranged by size:
3401 872 747 432 268
AflIII A'CryG_T
Cuts at: 3072 4176 5153 3072
Size: 1104 977 3639
Fragments arranged by size:
3639 1104 977
AgeI A'CCGG_T
Cuts at: 4108 4108
Size: 5720
AleI CACnn'nnGTG
Cuts at: 1921 1921
Size: 5720
AlwNI CAG_nnn'CTG
Cuts at: 1216 2297 2455 3025 4592 1216
Size: 1081 158 570 1567 2344
Fragments arranged by size:
2344 1567 1081 570 158
ApaI G_GGCC'C
Cuts at: 2189 2189
Size: 5720
ApaLI G'TGCA_C
Cuts at: 4490 4490
Size: 5720
ApoI r'AATT_y
Cuts at: 302 597 2002 2669 2724 5073 5329 302
Size: 295 1405 667 55 2349 256 693
Fragments arranged by size:
2349 1405 693 667 295 256 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 5720
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5720
AvaI C'yCGr_G
Cuts at: 694 2740 694
Size: 2046 3674
BamHI G'GATC_C
Cuts at: 746 2745 746
Size: 1999 3721
BanI G'GyrC_C
Cuts at: 1887 2736 3150 3398 3433 1887
Size: 849 414 248 35 4174
Fragments arranged by size:
4174 849 414 248 35
BanII G_rGCy'C
Cuts at: 119 450 759 1198 1319 1736 2169 2189 2734
Size: 331 309 439 121 417 433 20 545
Cuts at: 2734 3764 119
Size: 1030 2075
Fragments arranged by size:
2075 1030 545 439 433 417 331 309
121 20
BbeI G_GCGC'C
Cuts at: 3154 3402 3154
Size: 248 5472
BbsI GAAGACnn'nnnn_
Cuts at: 383 1360 383
Size: 977 4743
BbvCI CC'TCA_GC
Cuts at: 1217 1217
Size: 5720
BciVI GTATCCnnnnn_n'
Cuts at: 1826 3618 4379 5720 1826
Size: 1792 761 1341 1826
Fragments arranged by size:
1826 1792 1341 761
BclI T'GATC_A
Cuts at: 3240 3240
Size: 5720
BfrBI ATG'CAT
Cuts at: 953 1386 1428 953
Size: 433 42 5245
Fragments arranged by size:
5245 433 42
BglI GCCn_nnn'nGGC
Cuts at: 1996 2306 1996
Size: 310 5410
BglII A'GATC_T
Cuts at: 2489 3235 5272 2489
Size: 746 2037 2937
Fragments arranged by size:
2937 2037 746
BlpI GC'TnA_GC
Cuts at: 1804 2207 2832 1804
Size: 403 625 4692
Fragments arranged by size:
4692 625 403
Bme1580I G_kGCm'C
Cuts at: 1890 2189 3345 3438 4494 1890
Size: 299 1156 93 1056 3116
Fragments arranged by size:
3116 1156 1056 299 93
BmrI ACTGGGnnnn_n'
Cuts at: 1056 1192 2642 2932 3013 3348 5492 1056
Size: 136 1450 290 81 335 2144 1284
Fragments arranged by size:
2144 1450 1284 335 290 136 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2511 4087 2511
Size: 1576 4144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1280 1831 2160 2331 1280
Size: 551 329 171 4669
Fragments arranged by size:
4669 551 329 171
Bpu10I CC'TnA_GC
Cuts at: 1217 2168 1217
Size: 951 4769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 651 894 2896 3775 4267 4565 651
Size: 243 2002 879 492 298 1806
Fragments arranged by size:
2002 1806 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 2178 5424 2178
Size: 3246 2474
BsaAI yAC'GTr
Cuts at: 2550 3073 2550
Size: 523 5197
BsaBI GATnn'nnATC
Cuts at: 6 3234 5271 6
Size: 3228 2037 455
Fragments arranged by size:
3228 2037 455
BsaHI Gr'CG_yC
Cuts at: 1507 3151 3399 4101 5597 1507
Size: 1644 248 702 1496 1630
Fragments arranged by size:
1644 1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2909 3430 4108 4382 4529 4940 2909
Size: 521 678 274 147 411 3689
Fragments arranged by size:
3689 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 468 498 2554 2584 416
Size: 30 22 30 2056 30 3552
Fragments arranged by size:
3552 2056 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 346 440 1156 1539 1542 2548 194
Size: 152 94 716 383 3 1006 3366
Fragments arranged by size:
3366 1006 716 383 152 94 3
BseYI C'CCAG_C
Cuts at: 272 1420 3145 3903 4480 4976 272
Size: 1148 1725 758 577 496 1016
Fragments arranged by size:
1725 1148 1016 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 387 784 1272 2154 275
Size: 112 397 488 882 3841
Fragments arranged by size:
3841 882 488 397 112
BsiEI CG_ry'CG
Cuts at: 104 3262 3308 4516 104
Size: 3158 46 1208 1308
Fragments arranged by size:
3158 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 119 1319 1736 2734 3512 3702 4494 119
Size: 1200 417 998 778 190 792 1345
Fragments arranged by size:
1345 1200 998 792 778 417 190
BsmI GAATG_Cn'
Cuts at: 818 1411 818
Size: 593 5127
Bsp1286I G_dGCh'C
Cuts at: 119 450 759 1198 1319 1736 1890 2169 2189
Size: 331 309 439 121 417 154 279 20
Cuts at: 2189 2734 3345 3438 3512 3702 3764 4494 119
Size: 545 611 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 545 439 417 331 309
279 190 154 121 93 74 62 20
BspEI T'CCGG_A
Cuts at: 4940 4940
Size: 5720
BspHI T'CATG_A
Cuts at: 4896 4896
Size: 5720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2220 2770 2984 3286 3667 2220
Size: 550 214 302 381 4273
Fragments arranged by size:
4273 550 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1782 4012 4066 5550 5715 50
Size: 1732 2230 54 1484 165 55
Fragments arranged by size:
2230 1732 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2219 3632 2219
Size: 1413 4307
BsrFI r'CCGG_y
Cuts at: 1205 3718 3899 4108 1205
Size: 2513 181 209 2817
Fragments arranged by size:
2817 2513 209 181
BsrGI T'GTAC_A
Cuts at: 805 2600 805
Size: 1795 3925
BssSI C'ACGA_G
Cuts at: 3991 4349 3991
Size: 358 5362
BstAPI GCAn_nnn'nTGC
Cuts at: 398 1102 3097 398
Size: 704 1995 3021
Fragments arranged by size:
3021 1995 704
BstBI TT'CG_AA
Cuts at: 4081 4081
Size: 5720
BstEII G'GTnAC_C
Cuts at: 2089 2089
Size: 5720
BstXI CCAn_nnnn'nTGG
Cuts at: 2232 2232
Size: 5720
BstYI r'GATC_y
Cuts at: 1 746 1375 2489 2745 3212 3235 3570 3816
Size: 745 629 1114 256 467 23 335 246
Cuts at: 3816 4828 4914 4926 5272 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1114 1012 745 629 467 449 346 335
256 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1139 1139
Size: 5720
BtgI C'CryG_G
Cuts at: 1439 2591 1439
Size: 1152 4568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3670 3851 4014 3670
Size: 181 163 5376
Fragments arranged by size:
5376 181 163
BtsI GCAGTG_nn'
Cuts at: 263 1720 2963 263
Size: 1457 1243 3020
Fragments arranged by size:
3020 1457 1243
ClaI AT'CG_AT
Cuts at: 672 672
Size: 5720
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 189 224 189
Size: 35 5685
DraI TTT'AAA
Cuts at: 2719 2719
Size: 5720
DraIII CAC_nnn'GTG
Cuts at: 2130 2132 2130
Size: 2 5718
DrdI GACnn_nn'nnGTC
Cuts at: 3426 4284 3426
Size: 858 4862
EaeI y'GGCC_r
Cuts at: 407 1207 3305 3479 3870 3897 5242 407
Size: 800 2098 174 391 27 1345 885
Fragments arranged by size:
2098 1345 885 800 391 174 27
EagI C'GGCC_G
Cuts at: 3305 3305
Size: 5720
EarI CTCTTCn'nnn_
Cuts at: 1324 1343 1560 3743 3953 1324
Size: 19 217 2183 210 3091
Fragments arranged by size:
3091 2183 217 210 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3928 4238 4384 5530 3928
Size: 310 146 1146 4118
Fragments arranged by size:
4118 1146 310 146
EcoICRI GAG'CTC
Cuts at: 117 1317 1734 2732 117
Size: 1200 417 998 3105
Fragments arranged by size:
3105 1200 998 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 1280 1831 2160 2331 3545 3977 4724 4992
Size: 1136 551 329 171 1214 432 747 268
Cuts at: 4992 144
Size: 872
Fragments arranged by size:
1214 1136 872 747 551 432 329 268
171
EcoO109I rG'GnC_Cy
Cuts at: 1434 2186 2859 1434
Size: 752 673 4295
Fragments arranged by size:
4295 752 673
EcoRI G'AATT_C
Cuts at: 2669 2724 2669
Size: 55 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1123 1335 3739 1123
Size: 212 2404 3104
Fragments arranged by size:
3104 2404 212
FspI TGC'GCA
Cuts at: 1326 3501 1326
Size: 2175 3545
HaeII r_GCGC'y
Cuts at: 3154 3402 4424 5403 3154
Size: 248 1022 979 3471
Fragments arranged by size:
3471 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 104 136 3562 3592 3594 3624 104
Size: 32 3426 30 2 30 2200
Fragments arranged by size:
3426 2200 32 30 30 2
HincII GTy'rAC
Cuts at: 123 2759 123
Size: 2636 3084
HindIII A'AGCT_T
Cuts at: 1414 1414
Size: 5720
KasI G'GCGC_C
Cuts at: 3150 3398 3150
Size: 248 5472
KpnI G_GTAC'C
Cuts at: 2740 2740
Size: 5720
MluI A'CGCG_T
Cuts at: 5153 5153
Size: 5720
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2389 2602 4391 4575 2389
Size: 213 1789 184 3534
Fragments arranged by size:
3534 1789 213 184
MscI TGG'CCA
Cuts at: 3481 3481
Size: 5720
MslI CAynn'nnrTG
Cuts at: 493 1921 2320 3709 3836 493
Size: 1428 399 1389 127 2377
Fragments arranged by size:
2377 1428 1389 399 127
MspA1I CmG'CkG
Cuts at: 164 921 1213 2239 2831 3145 3505 4518 4763
Size: 757 292 1026 592 314 360 1013 245
Cuts at: 4763 164
Size: 1121
Fragments arranged by size:
1121 1026 1013 757 592 360 314 292
245
NaeI GCC'GGC
Cuts at: 1207 3901 1207
Size: 2694 3026
NarI GG'CG_CC
Cuts at: 3151 3399 3151
Size: 248 5472
NgoMIV G'CCGG_C
Cuts at: 1205 3899 1205
Size: 2694 3026
NsiI A_TGCA'T
Cuts at: 955 1388 1430 955
Size: 433 42 5245
Fragments arranged by size:
5245 433 42
NspI r_CATG'y
Cuts at: 953 1428 2118 2685 2773 3273 4180 953
Size: 475 690 567 88 500 907 2493
Fragments arranged by size:
2493 907 690 567 500 475 88
PasI CC'CwG_GG
Cuts at: 2182 2182
Size: 5720
PciI A'CATG_T
Cuts at: 4176 4176
Size: 5720
PflMI CCAn_nnn'nTGG
Cuts at: 839 839
Size: 5720
PfoI T'CCnGG_A
Cuts at: 286 338 1244 1257 2347 286
Size: 52 906 13 1090 3659
Fragments arranged by size:
3659 1090 906 52 13
PmeI GTTT'AAAC
Cuts at: 2719 2719
Size: 5720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2376 4859 4891 2344
Size: 32 2483 32 3173
Fragments arranged by size:
3173 2483 32 32
PpuMI rG'GwC_Cy
Cuts at: 1434 1434
Size: 5720
PsiI TTA'TAA
Cuts at: 1472 2435 1472
Size: 963 4757
PspOMI G'GGCC_C
Cuts at: 2185 2185
Size: 5720
PstI C_TGCA'G
Cuts at: 920 1002 2510 2640 2767 3452 920
Size: 82 1508 130 127 685 3188
Fragments arranged by size:
3188 1508 685 130 127 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5720
PvuII CAG'CTG
Cuts at: 921 1213 2239 3145 3505 921
Size: 292 1026 906 360 3136
Fragments arranged by size:
3136 1026 906 360 292
RsrII CG'GwC_CG
Cuts at: 3915 3915
Size: 5720
SacI G_AGCT'C
Cuts at: 119 1319 1736 2734 119
Size: 1200 417 998 3105
Fragments arranged by size:
3105 1200 998 417
SalI G'TCGA_C
Cuts at: 2757 2757
Size: 5720
SanDI GG'GwC_CC
Cuts at: 1434 1434
Size: 5720
SapI GCTCTTCn'nnn_
Cuts at: 1324 3743 3953 1324
Size: 2419 210 3091
Fragments arranged by size:
3091 2419 210
SbfI CC_TGCA'GG
Cuts at: 2767 2767
Size: 5720
ScaI AGT'ACT
Cuts at: 987 1297 5237 987
Size: 310 3940 1470
Fragments arranged by size:
3940 1470 310
SfcI C'TryA_G
Cuts at: 33 916 998 1371 2447 2506 2636 2763 3448
Size: 883 82 373 1076 59 130 127 685
Cuts at: 3448 4441 4632 33
Size: 993 191 1121
Fragments arranged by size:
1121 1076 993 883 685 373 191 130
127 82 59
SfoI GGC'GCC
Cuts at: 3152 3400 3152
Size: 248 5472
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5720
SmaI CCC'GGG
Cuts at: 2742 2742
Size: 5720
SmlI C'TyrA_G
Cuts at: 630 909 2875 3790 4282 4544 630
Size: 279 1966 915 492 262 1806
Fragments arranged by size:
1966 1806 915 492 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5720
SphI G_CATG'C
Cuts at: 1428 2773 3273 1428
Size: 1345 500 3875
Fragments arranged by size:
3875 1345 500
SspI AAT'ATT
Cuts at: 1625 1625
Size: 5720
StyI C'CwwG_G
Cuts at: 992 1819 2854 3208 992
Size: 827 1035 354 3504
Fragments arranged by size:
3504 1035 827 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 911 3303 3436 3895 4104 911
Size: 2392 133 459 209 2527
Fragments arranged by size:
2527 2392 459 209 133
TatI w'GTAC_w
Cuts at: 532 805 985 1064 1295 2600 5235 532
Size: 273 180 79 231 1305 2635 1017
Fragments arranged by size:
2635 1305 1017 273 231 180 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 416 448 2668 2700 5237 5269 416
Size: 32 2220 32 2537 32 867
Fragments arranged by size:
2537 2220 867 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3517 3517
Size: 5720
XbaI T'CTAG_A
Cuts at: 1464 2751 1464
Size: 1287 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 417 563 2298 3215 369
Size: 48 146 1735 917 2874
Fragments arranged by size:
2874 1735 917 146 48
XmaI C'CCGG_G
Cuts at: 2740 2740
Size: 5720
ZraI GAC'GTC
Cuts at: 1508 1508
Size: 5720
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BmgBI BmtI BsiWI BsmBI BssHII BstZ17I EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PmlI PshAI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI