Restriction map for pF1KE2398
 (Linear) (Six-base) MAPSORT of: KIEE2398.seq  Check: 3429  from: 1  to: 1014



KIEE2398.orf

 With 182 enzymes: SgfI * 

                        February 7, 2012 15:11  ..

AatII G_ACGT'C

Cuts at:      0     770    1014 
   Size:        770     244
 
AfeI AGC'GCT

Cuts at:      0     508    1014 
   Size:        508     506
 
AgeI A'CCGG_T

Cuts at:      0     533    1014 
   Size:        533     481
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     564    1014 
   Size:        564     450
 
ApaI G_GGCC'C

Cuts at:      0     738    1014 
   Size:        738     276
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     240     273    1014 
   Size:        240      33     741

  Fragments arranged by size: 

                 741     240      33
 
BamHI G'GATC_C

Cuts at:      0     167    1014 
   Size:        167     847
 
BanII G_rGCy'C

Cuts at:      0     270     738    1008    1014 
   Size:        270     468     270       6

  Fragments arranged by size: 

                 468     270     270       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     214    1014 
   Size:        214     800
 
BclI T'GATC_A

Cuts at:      0     329    1014 
   Size:        329     685
 
BglI GCCn_nnn'nGGC

Cuts at:      0      68     887    1014 
   Size:         68     819     127

  Fragments arranged by size: 

                 819     127      68
 
Bme1580I G_kGCm'C

Cuts at:      0     738    1014 
   Size:        738     276
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     240    1014 
   Size:        240     774
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     321    1014 
   Size:        193     128     693

  Fragments arranged by size: 

                 693     193     128
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256    1014 
   Size:        256     758
 
BsaHI Gr'CG_yC

Cuts at:      0      86     317     767    1014 
   Size:         86     231     450     247

  Fragments arranged by size: 

                 450     247     231      86
 
BsaWI w'CCGG_w

Cuts at:      0     533     554     593     686     857    1014 
   Size:        533      21      39      93     171     157

  Fragments arranged by size: 

                 533     171     157      93      39      21
 
BseYI C'CCAG_C

Cuts at:      0     445    1014 
   Size:        445     569
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     359    1014 
   Size:        359     655
 
BsiEI CG_ry'CG

Cuts at:      0     749     831    1014 
   Size:        749      82     183

  Fragments arranged by size: 

                 749     183      82
 
BsiHKAI G_wGCw'C

Cuts at:      0     179    1008    1014 
   Size:        179     829       6

  Fragments arranged by size: 

                 829     179       6
 
BsmI GAATG_Cn'

Cuts at:      0     221    1014 
   Size:        221     793
 
Bsp1286I G_dGCh'C

Cuts at:      0     179     270     738    1008    1014 
   Size:        179      91     468     270       6

  Fragments arranged by size: 

                 468     270     179      91       6
 
BspEI T'CCGG_A

Cuts at:      0     554     857    1014 
   Size:        554     303     157

  Fragments arranged by size: 

                 554     303     157
 
BspHI T'CATG_A

Cuts at:      0     326    1014 
   Size:        326     688
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122     663    1014 
   Size:        122     541     351

  Fragments arranged by size: 

                 541     351     122
 
BsrFI r'CCGG_y

Cuts at:      0     533     830     886    1014 
   Size:        533     297      56     128

  Fragments arranged by size: 

                 533     297     128      56
 
BsrGI T'GTAC_A

Cuts at:      0     518    1014 
   Size:        518     496
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     510    1014 
   Size:        510     504
 
BstYI r'GATC_y

Cuts at:      0     167     234    1014 
   Size:        167      67     780

  Fragments arranged by size: 

                 780     167      67
 
BtgI C'CryG_G

Cuts at:      0     743    1014 
   Size:        743     271
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     172    1014 
   Size:        172     842
 
DraI TTT'AAA

Cuts at:      0     395    1014 
   Size:        395     619
 
EaeI y'GGCC_r

Cuts at:      0     161     455     728     746     815     828     845     884 
   Size:        161     294     273      18      69      13      17      39

Cuts at:    884    1014 
   Size:        130

  Fragments arranged by size: 

                 294     273     161     130      69      39      18      17
                  13
 
EagI C'GGCC_G

Cuts at:      0     746     828    1014 
   Size:        746      82     186

  Fragments arranged by size: 

                 746     186      82
 
EcoICRI GAG'CTC

Cuts at:      0    1006    1014 
   Size:       1006       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     321    1014 
   Size:        193     128     693

  Fragments arranged by size: 

                 693     193     128
 
EcoO109I rG'GnC_Cy

Cuts at:      0     305     584     734     735    1014 
   Size:        305     279     150       1     279

  Fragments arranged by size: 

                 305     279     279     150       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     525    1014 
   Size:        525     489
 
FspI TGC'GCA

Cuts at:      0      10    1014 
   Size:         10    1004
 
HaeII r_GCGC'y

Cuts at:      0     128     510     990    1014 
   Size:        128     382     480      24

  Fragments arranged by size: 

                 480     382     128      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     219     245     251    1014 
   Size:        213       6      26       6     763

  Fragments arranged by size: 

                 763     213      26       6       6
 
HincII GTy'rAC

Cuts at:      0     321     678    1014 
   Size:        321     357     336

  Fragments arranged by size: 

                 357     336     321
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77    1014 
   Size:         77     937
 
MscI TGG'CCA

Cuts at:      0     457     817     847    1014 
   Size:        457     360      30     167

  Fragments arranged by size: 

                 457     360     167      30
 
MslI CAynn'nnrTG

Cuts at:      0     142     331    1014 
   Size:        142     189     683

  Fragments arranged by size: 

                 683     189     142
 
MspA1I CmG'CkG

Cuts at:      0      14     548     612     745     827     993    1014 
   Size:         14     534      64     133      82     166      21

  Fragments arranged by size: 

                 534     166     133      82      64      21      14
 
NaeI GCC'GGC

Cuts at:      0     832     888    1014 
   Size:        832      56     126

  Fragments arranged by size: 

                 832     126      56
 
NgoMIV G'CCGG_C

Cuts at:      0     830     886    1014 
   Size:        830      56     128

  Fragments arranged by size: 

                 830     128      56
 
NotI GC'GGCC_GC

Cuts at:      0     746    1014 
   Size:        746     268
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36     947    1014 
   Size:         36     911      67

  Fragments arranged by size: 

                 911      67      36
 
PfoI T'CCnGG_A

Cuts at:      0     170    1014 
   Size:        170     844
 
PspOMI G'GGCC_C

Cuts at:      0     734    1014 
   Size:        734     280
 
PstI C_TGCA'G

Cuts at:      0     668    1014 
   Size:        668     346
 
PvuII CAG'CTG

Cuts at:      0     548     612     993    1014 
   Size:        548      64     381      21

  Fragments arranged by size: 

                 548     381      64      21
 
RsrII CG'GwC_CG

Cuts at:      0     287    1014 
   Size:        287     727
 
SacI G_AGCT'C

Cuts at:      0    1008    1014 
   Size:       1008       6
 
SacII CC_GC'GG

Cuts at:      0     746    1014 
   Size:        746     268
 
SbfI CC_TGCA'GG

Cuts at:      0     668    1014 
   Size:        668     346
 
ScaI AGT'ACT

Cuts at:      0     973    1014 
   Size:        973      41
 
SfcI C'TryA_G

Cuts at:      0     664    1014 
   Size:        664     350
 
SmlI C'TyrA_G

Cuts at:      0     271    1014 
   Size:        271     743
 
StyI C'CwwG_G

Cuts at:      0     414    1014 
   Size:        414     600
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     154     171     304     325     586    1014 
   Size:        154      17     133      21     261     428

  Fragments arranged by size: 

                 428     261     154     133      21      17
 
TatI w'GTAC_w

Cuts at:      0     248     518     971    1014 
   Size:        248     270     453      43

  Fragments arranged by size: 

                 453     270     248      43
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     759     990    1014 
   Size:        759     231      24

  Fragments arranged by size: 

                 759     231      24
 
ZraI GAC'GTC

Cuts at:      0     768    1014 
   Size:        768     246
 

 Enzymes that do cut:

    AatII     AfeI     AgeI     AlfI     ApaI     BaeI    BamHI    BanII
     BbsI     BclI     BglI Bme1580I     BmrI     BpmI    BpuEI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrFI    BsrGI   BstAPI    BstYI     BtgI    BtgZI
     DraI     EaeI     EagI  EcoICRI  Eco57MI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NotI    PflMI     PfoI   PspOMI     PstI    PvuII    RsrII
     SacI    SacII     SbfI     ScaI     SfcI     SmlI     StyI    TaqII
     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AhdI
     AleI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaXI    BseRI    BsiWI    BsmBI    BsrBI    BsrDI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI   DraIII     DrdI     EarI     EciI    EcoNI    EcoRI    EcoRV
     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI     SalI    SanDI     SapI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI
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