(Circular) (Six-base) MAPSORT of: pF1KE3542.seq Check: 2449 from: 1 to: 6503 pF1KE3542 6503 bp With 182 enzymes: SgfI * March 22, 2013 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 192 340 1794 2296 192 Size: 148 1454 502 4399 Fragments arranged by size: 4399 1454 502 148 AccI GT'mk_AC Cuts at: 823 3541 823 Size: 2718 3785 Acc65I G'GTAC_C Cuts at: 3519 3519 Size: 6503 AclI AA'CG_TT Cuts at: 6343 6343 Size: 6503 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1130 1370 4328 4760 5507 5775 1130 Size: 240 2958 432 747 268 1858 Fragments arranged by size: 2958 1858 747 432 268 240 AflII C'TTAA_G Cuts at: 1903 1903 Size: 6503 AflIII A'CryG_T Cuts at: 3855 4959 5936 3855 Size: 1104 977 4422 Fragments arranged by size: 4422 1104 977 AgeI A'CCGG_T Cuts at: 4891 4891 Size: 6503 AhdI GACnn_n'nnGTC Cuts at: 1605 3433 1605 Size: 1828 4675 AleI CACnn'nnGTG Cuts at: 3217 3217 Size: 6503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1802 1802 Size: 6503 AlwNI CAG_nnn'CTG Cuts at: 2875 3808 5375 2875 Size: 933 1567 4003 Fragments arranged by size: 4003 1567 933 ApaLI G'TGCA_C Cuts at: 5273 5273 Size: 6503 ApoI r'AATT_y Cuts at: 412 686 703 805 905 1975 1981 2251 2279 Size: 274 17 102 100 1070 6 270 28 Cuts at: 2279 2311 2944 3154 3507 5856 6112 412 Size: 32 633 210 353 2349 256 803 Fragments arranged by size: 2349 1070 803 633 353 274 270 256 210 102 100 32 28 17 6 AseI AT'TA_AT Cuts at: 20 20 Size: 6503 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6503 AvaI C'yCGr_G Cuts at: 220 244 2190 3523 220 Size: 24 1946 1333 3200 Fragments arranged by size: 3200 1946 1333 24 AvrII C'CTAG_G Cuts at: 379 1393 379 Size: 1014 5489 BamHI G'GATC_C Cuts at: 2659 3528 2659 Size: 869 5634 BanI G'GyrC_C Cuts at: 147 239 3519 3933 4181 4216 147 Size: 92 3280 414 248 35 2434 Fragments arranged by size: 3280 2434 414 248 92 35 BanII G_rGCy'C Cuts at: 142 1447 3517 4547 142 Size: 1305 2070 1030 2098 Fragments arranged by size: 2098 2070 1305 1030 BbeI G_GCGC'C Cuts at: 151 243 3937 4185 151 Size: 92 3694 248 2469 Fragments arranged by size: 3694 2469 248 92 BbsI GAAGACnn'nnnn_ Cuts at: 302 2234 2347 302 Size: 1932 113 4458 Fragments arranged by size: 4458 1932 113 BbvCI CC'TCA_GC Cuts at: 2952 2952 Size: 6503 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 160 194 823 857 90 Size: 34 36 34 629 34 5736 Fragments arranged by size: 5736 629 36 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1948 4401 5162 6503 1948 Size: 2453 761 1341 1948 Fragments arranged by size: 2453 1948 1341 761 BclI T'GATC_A Cuts at: 1574 2099 4023 1574 Size: 525 1924 4054 Fragments arranged by size: 4054 1924 525 BfrBI ATG'CAT Cuts at: 2965 2965 Size: 6503 BglII A'GATC_T Cuts at: 1473 4018 6055 1473 Size: 2545 2037 1921 Fragments arranged by size: 2545 2037 1921 BlpI GC'TnA_GC Cuts at: 3615 3615 Size: 6503 Bme1580I G_kGCm'C Cuts at: 214 3207 4128 4221 5277 214 Size: 2993 921 93 1056 1440 Fragments arranged by size: 2993 1440 1056 921 93 BmgBI CAC'GTC Cuts at: 300 477 300 Size: 177 6326 BmrI ACTGGGnnnn_n' Cuts at: 200 529 1755 1877 2114 2359 3194 3715 3796 Size: 329 1226 122 237 245 835 521 81 Cuts at: 3796 4131 6275 200 Size: 335 2144 428 Fragments arranged by size: 2144 1226 835 521 428 335 329 245 237 122 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1685 4870 1685 Size: 3185 3318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 110 2832 110 Size: 2722 3781 Bpu10I CC'TnA_GC Cuts at: 2952 2952 Size: 6503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2259 3679 4558 5050 5348 2259 Size: 1420 879 492 298 3414 Fragments arranged by size: 3414 1420 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 2774 2848 3006 6207 2774 Size: 74 158 3201 3070 Fragments arranged by size: 3201 3070 158 74 BsaAI yAC'GTr Cuts at: 1614 3856 1614 Size: 2242 4261 BsaBI GATnn'nnATC Cuts at: 6 4017 6054 6 Size: 4011 2037 455 Fragments arranged by size: 4011 2037 455 BsaHI Gr'CG_yC Cuts at: 148 240 3934 4182 4884 6380 148 Size: 92 3694 248 702 1496 271 Fragments arranged by size: 3694 1496 702 271 248 92 BsaWI w'CCGG_w Cuts at: 3261 3692 4213 4891 5165 5312 5723 3261 Size: 431 521 678 274 147 411 4041 Fragments arranged by size: 4041 678 521 431 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 649 679 2983 3013 649 Size: 30 2304 30 4139 Fragments arranged by size: 4139 2304 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 150 156 286 295 298 301 1059 2062 2940 Size: 6 130 9 3 3 758 1003 878 Cuts at: 2940 150 Size: 3713 Fragments arranged by size: 3713 1003 878 758 130 9 6 3 3 BseYI C'CCAG_C Cuts at: 1252 2530 3928 4686 5263 5759 1252 Size: 1278 1398 758 577 496 1996 Fragments arranged by size: 1996 1398 1278 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 164 164 Size: 6503 BsiEI CG_ry'CG Cuts at: 104 4045 4091 5299 104 Size: 3941 46 1208 1308 Fragments arranged by size: 3941 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 142 3517 4295 4485 5277 142 Size: 3375 778 190 792 1368 Fragments arranged by size: 3375 1368 792 778 190 BsmI GAATG_Cn' Cuts at: 2726 2726 Size: 6503 BsmBI CGTCTCn'nnnn_ Cuts at: 483 3420 483 Size: 2937 3566 Bsp1286I G_dGCh'C Cuts at: 142 214 1447 3207 3517 4128 4221 4295 4485 Size: 72 1233 1760 310 611 93 74 190 Cuts at: 4485 4547 5277 142 Size: 62 730 1368 Fragments arranged by size: 1760 1368 1233 730 611 310 190 93 74 72 62 BspEI T'CCGG_A Cuts at: 3261 5723 3261 Size: 2462 4041 BspHI T'CATG_A Cuts at: 5679 5679 Size: 6503 BspMI ACCTGCnnnn'nnnn_ Cuts at: 192 340 1794 2296 3553 3767 4069 4450 192 Size: 148 1454 502 1257 214 302 381 2245 Fragments arranged by size: 2245 1454 1257 502 381 302 214 148 BsrBI CCG'CTC Cuts at: 50 4795 4849 6333 6498 50 Size: 4745 54 1484 165 55 Fragments arranged by size: 4745 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 971 1646 1763 2732 4415 971 Size: 675 117 969 1683 3059 Fragments arranged by size: 3059 1683 969 675 117 BsrFI r'CCGG_y Cuts at: 4501 4682 4891 4501 Size: 181 209 6113 Fragments arranged by size: 6113 209 181 BssSI C'ACGA_G Cuts at: 207 2035 4774 5132 207 Size: 1828 2739 358 1578 Fragments arranged by size: 2739 1828 1578 358 BstAPI GCAn_nnn'nTGC Cuts at: 2580 2962 3880 2580 Size: 382 918 5203 Fragments arranged by size: 5203 918 382 BstBI TT'CG_AA Cuts at: 4864 4864 Size: 6503 BstXI CCAn_nnnn'nTGG Cuts at: 2514 2856 2514 Size: 342 6161 BstYI r'GATC_y Cuts at: 1 662 1473 2360 2494 2593 2659 3528 3995 Size: 661 811 887 134 99 66 869 467 Cuts at: 3995 4018 4353 4599 5611 5697 5709 6055 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 887 869 811 661 467 449 346 335 246 134 99 86 66 23 12 BstZ17I GTA'TAC Cuts at: 824 824 Size: 6503 Bsu36I CC'TnA_GG Cuts at: 2539 2539 Size: 6503 BtgI C'CryG_G Cuts at: 389 733 795 1700 3083 389 Size: 344 62 905 1383 3809 Fragments arranged by size: 3809 1383 905 344 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2916 2975 4453 4634 4797 2916 Size: 59 1478 181 163 4622 Fragments arranged by size: 4622 1478 181 163 59 BtsI GCAGTG_nn' Cuts at: 3120 3746 3120 Size: 626 5877 DraI TTT'AAA Cuts at: 2555 3502 2555 Size: 947 5556 DraIII CAC_nnn'GTG Cuts at: 1453 1453 Size: 6503 DrdI GACnn_nn'nnGTC Cuts at: 4209 5067 4209 Size: 858 5645 EaeI y'GGCC_r Cuts at: 457 1031 2391 2504 2691 4088 4262 4653 4680 Size: 574 1360 113 187 1397 174 391 27 Cuts at: 4680 6025 457 Size: 1345 935 Fragments arranged by size: 1397 1360 1345 935 574 391 187 174 113 27 EagI C'GGCC_G Cuts at: 4088 4088 Size: 6503 EarI CTCTTCn'nnn_ Cuts at: 272 1096 1106 1866 2141 2146 2406 4526 4736 Size: 824 10 760 275 5 260 2120 210 Cuts at: 4736 272 Size: 2039 Fragments arranged by size: 2120 2039 824 760 275 260 210 10 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2075 4711 5021 5167 6313 2075 Size: 2636 310 146 1146 2265 Fragments arranged by size: 2636 2265 1146 310 146 EcoICRI GAG'CTC Cuts at: 140 3515 140 Size: 3375 3128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 110 1130 1370 2832 4328 4760 5507 5775 110 Size: 1020 240 1462 1496 432 747 268 838 Fragments arranged by size: 1496 1462 1020 838 747 432 268 240 EcoNI CCTnn'n_nnAGG Cuts at: 231 3220 231 Size: 2989 3514 EcoO109I rG'GnC_Cy Cuts at: 224 309 919 2568 3642 224 Size: 85 610 1649 1074 3085 Fragments arranged by size: 3085 1649 1074 610 85 EcoRI G'AATT_C Cuts at: 2279 3507 2279 Size: 1228 5275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3348 4522 3348 Size: 1174 5329 FspI TGC'GCA Cuts at: 4284 4284 Size: 6503 HaeII r_GCGC'y Cuts at: 151 243 3937 4185 5207 6186 151 Size: 92 3694 248 1022 979 468 Fragments arranged by size: 3694 1022 979 468 248 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1054 1086 1458 1490 1591 1623 1689 1721 1735 Size: 32 372 32 101 32 66 32 14 Cuts at: 1735 1767 2018 2050 2054 2086 2644 2676 2983 Size: 32 251 32 4 32 558 32 307 Cuts at: 2983 3015 3180 3212 3419 3451 4345 4375 4377 Size: 32 165 32 207 32 894 30 2 Cuts at: 4377 4407 1054 Size: 30 3150 Fragments arranged by size: 3150 894 558 372 307 251 207 165 101 66 32 32 32 32 32 32 32 32 32 32 32 30 30 14 4 2 HincII GTy'rAC Cuts at: 3542 3542 Size: 6503 HindIII A'AGCT_T Cuts at: 1244 1244 Size: 6503 KasI G'GCGC_C Cuts at: 147 239 3933 4181 147 Size: 92 3694 248 2469 Fragments arranged by size: 3694 2469 248 92 KpnI G_GTAC'C Cuts at: 3523 3523 Size: 6503 MfeI C'AATT_G Cuts at: 839 839 Size: 6503 MluI A'CGCG_T Cuts at: 5936 5936 Size: 6503 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 506 2697 5174 5358 506 Size: 2191 2477 184 1651 Fragments arranged by size: 2477 2191 1651 184 MscI TGG'CCA Cuts at: 459 1033 2393 2693 4264 459 Size: 574 1360 300 1571 2698 Fragments arranged by size: 2698 1571 1360 574 300 MslI CAynn'nnrTG Cuts at: 574 1936 2512 2983 3217 4492 4619 574 Size: 1362 576 471 234 1275 127 2458 Fragments arranged by size: 2458 1362 1275 576 471 234 127 MspA1I CmG'CkG Cuts at: 178 1851 2053 3111 3614 3928 4288 5301 5546 Size: 1673 202 1058 503 314 360 1013 245 Cuts at: 5546 178 Size: 1135 Fragments arranged by size: 1673 1135 1058 1013 503 360 314 245 202 NaeI GCC'GGC Cuts at: 4684 4684 Size: 6503 NarI GG'CG_CC Cuts at: 148 240 3934 4182 148 Size: 92 3694 248 2469 Fragments arranged by size: 3694 2469 248 92 NcoI C'CATG_G Cuts at: 389 733 3083 389 Size: 344 2350 3809 Fragments arranged by size: 3809 2350 344 NdeI CA'TA_TG Cuts at: 2223 2223 Size: 6503 NgoMIV G'CCGG_C Cuts at: 4682 4682 Size: 6503 NsiI A_TGCA'T Cuts at: 2967 2967 Size: 6503 NspI r_CATG'y Cuts at: 1373 3556 4056 4963 1373 Size: 2183 500 907 2913 Fragments arranged by size: 2913 2183 907 500 PciI A'CATG_T Cuts at: 4959 4959 Size: 6503 PflMI CCAn_nnn'nTGG Cuts at: 553 2912 553 Size: 2359 4144 PfoI T'CCnGG_A Cuts at: 407 407 Size: 6503 PmeI GTTT'AAAC Cuts at: 3502 3502 Size: 6503 PmlI CAC'GTG Cuts at: 1614 1614 Size: 6503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5642 5674 5642 Size: 32 6471 PpuMI rG'GwC_Cy Cuts at: 224 309 224 Size: 85 6418 PshAI GACnn'nnGTC Cuts at: 470 470 Size: 6503 PstI C_TGCA'G Cuts at: 472 1186 3550 4235 472 Size: 714 2364 685 2740 Fragments arranged by size: 2740 2364 714 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6503 PvuII CAG'CTG Cuts at: 178 1851 3111 3928 4288 178 Size: 1673 1260 817 360 2393 Fragments arranged by size: 2393 1673 1260 817 360 RsrII CG'GwC_CG Cuts at: 4698 4698 Size: 6503 SacI G_AGCT'C Cuts at: 142 3517 142 Size: 3375 3128 SalI G'TCGA_C Cuts at: 3540 3540 Size: 6503 SapI GCTCTTCn'nnn_ Cuts at: 1106 4526 4736 1106 Size: 3420 210 2873 Fragments arranged by size: 3420 2873 210 SbfI CC_TGCA'GG Cuts at: 3550 3550 Size: 6503 ScaI AGT'ACT Cuts at: 1595 1828 6020 1595 Size: 233 4192 2078 Fragments arranged by size: 4192 2078 233 SexAI A'CCwGG_T Cuts at: 608 608 Size: 6503 SfcI C'TryA_G Cuts at: 33 468 923 1182 1965 2325 2937 3546 4231 Size: 435 455 259 783 360 612 609 685 Cuts at: 4231 5224 5415 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 783 685 612 609 455 435 360 259 191 SfoI GGC'GCC Cuts at: 149 241 3935 4183 149 Size: 92 3694 248 2469 Fragments arranged by size: 3694 2469 248 92 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6503 SmaI CCC'GGG Cuts at: 222 246 2192 3525 222 Size: 24 1946 1333 3200 Fragments arranged by size: 3200 1946 1333 24 SmlI C'TyrA_G Cuts at: 1903 2274 3658 4573 5065 5327 1903 Size: 371 1384 915 492 262 3079 Fragments arranged by size: 3079 1384 915 492 371 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6503 SphI G_CATG'C Cuts at: 3556 4056 3556 Size: 500 6003 StuI AGG'CCT Cuts at: 1571 1571 Size: 6503 StyI C'CwwG_G Cuts at: 379 389 733 1054 1393 3083 3637 3991 379 Size: 10 344 321 339 1690 554 354 2891 Fragments arranged by size: 2891 1690 554 354 344 339 321 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 545 865 4086 4219 4678 4887 545 Size: 320 3221 133 459 209 2161 Fragments arranged by size: 3221 2161 459 320 209 133 TatI w'GTAC_w Cuts at: 516 1186 1593 1826 6018 516 Size: 670 407 233 4192 1001 Fragments arranged by size: 4192 1001 670 407 233 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 654 686 6020 6052 654 Size: 32 5334 32 1105 Fragments arranged by size: 5334 1105 32 32 Tth111I GACn'n_nGTC Cuts at: 3166 4300 3166 Size: 1134 5369 XbaI T'CTAG_A Cuts at: 3534 3534 Size: 6503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 554 573 2170 3998 554 Size: 19 1597 1828 3059 Fragments arranged by size: 3059 1828 1597 19 XmaI C'CCGG_G Cuts at: 220 244 2190 3523 220 Size: 24 1946 1333 3200 Fragments arranged by size: 3200 1946 1333 24 XmnI GAAnn'nnTTC Cuts at: 3331 3331 Size: 6503 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AloI ApaI AscI BaeI BglI BmtI BsiWI BsrGI BssHII BstEII ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI NruI PacI PasI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI