################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AvrII BbeI BclI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FseI HaeII HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI PshBI PvuI RcaI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI StuI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 1244 GACGTC GACGT^C AclI 584 AACGTT AA^CGTT AcsI 2446 GAATTC [GA]^AATT[CT] AcyI 1244 GACGTC G[GA]^CG[CT]C AflIII 2063 ACACGT A^C[GA][CT]GT AocI 294 CCTCAGG CC^T[ACGT]AGG ApoI 2446 GAATTC [GA]^AATT[CT] AviII 47 TGCGCA TGC^GCA BamHI 1023 GGATCC G^GATCC BbrPI 2064 CACGTG CAC^GTG BbvCI 276 CCTCAGC CCTCAGC(-5/-2) BcgI 1983 CGAGACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2192 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 681 AGATCT A^GATCT BlpI 2158 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2158 GCTGAGC GC^T[ACGT]AGC BsaAI 2064 CACGTG [CT]AC^GT[GA] BsaHI 1244 GACGTC G[GA]^CG[CT]C BsgI 1539 GTGCAG GTGCAG(16/14) BsiEI 17 CGGCCG CG[GA][CT]^CG BsmI 2052 GCATTC GAATGC(1/-1) BsrDI 365 GCAATG GCAATG(2/0) BsrFI 19 GCCGGC [GA]^CCGG[CT] BstAPI 2043 GCACACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2690 CCAGGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 294 CCTCAGG CC^T[ACGT]AGG CelII 2158 GCTGAGC GC^T[ACGT]AGC Cfr10I 19 GCCGGC [GA]^CCGG[CT] DrdI 1723 GACGGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 17 CGGCCG C^GGCCG EclXI 17 CGGCCG C^GGCCG Eco52I 17 CGGCCG C^GGCCG Eco81I 294 CCTCAGG CC^T[ACGT]AGG EcoNI 315 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2446 GAATTC G^AATTC FspI 47 TGCGCA TGC^GCA Hin1I 1244 GACGTC G[GA]^CG[CT]C HindIII 1485 AAGCTT A^AGCTT KspI 31 CCGCGG CCGC^GG MslI 2623 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 19 GCCGGC GCC^GGC NcoI 2397 CCATGG C^CATGG NgoMIV 19 GCCGGC G^CCGGC NheI 639 GCTAGC G^CTAGC NspI 687 GCATGC [GA]CATG^[CT] PleI 2642 GACTC GAGTC(4/5) PmaCI 2064 CACGTG CAC^GTG PmlI 2064 CACGTG CAC^GTG Psp1406I 584 AACGTT AA^CGTT SacI 1336 GAGCTC GAGCT^C SacII 31 CCGCGG CCGC^GG SbfI 1127 CCTGCAGG CCTGCA^GG SexAI 767 ACCTGGT A^CC[AT]GGT SphI 687 GCATGC GCATG^C Sse8387I 1127 CCTGCAGG CCTGCA^GG XbaI 1059 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1633 GTAGAC GT^[AC][GT]AC 2023 GTCTAC Alw44I 1642 GTGCAC G^TGCAC 2041 GTGCAC AlwNI 1660 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2740 CAGAAGCTG ApaLI 1642 GTGCAC G^TGCAC 2041 GTGCAC Asp700I 582 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 774 GAAGTGGTTC AspI 1756 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2365 GACTGTGTC BalI 224 TGGCCA TGG^CCA 2552 TGGCCA BanI 1123 GGCACC G^G[CT][GA]CC 2250 GGTGCC BciVI 2428 GGATAC GTATCC(6/5) 2693 GGATAC BsaI 1813 GAGACC GGTCTC(1/5) 1821 GAGACC BsrBI 1412 CCGCTC CCGCTC(-3/-3) 1261 GAGCGG BstYI 681 AGATCT [GA]^GATC[CT] 1023 GGATCC CfoI 14 GCGC GCG^C 48 GCGC DraIII 1645 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2338 CACCGCGTG HgaI 38 GACGC GACGC(5/10) 2586 GCGTC HhaI 14 GCGC GCG^C 48 GCGC HinP1I 14 GCGC G^CGC 48 GCGC MflI 681 AGATCT [GA]^GATC[CT] 1023 GGATCC MluNI 224 TGGCCA TGG^CCA 2552 TGGCCA MscI 224 TGGCCA TGG^CCA 2552 TGGCCA MseI 239 TTAA T^TAA 800 TTAA PaeR7I 737 CTCGAG C^TCGAG 875 CTCGAG PflFI 1756 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2365 GACTGTGTC PpuMI 1453 AGGACCC [GA]G^G[AT]CC[CT] 1478 AGGACCC SapI 132 GAAGAGC GCTCTTC(1/4) 1508 GCTCTTC TfiI 912 GATTC G^A[AT]TC 1375 GATTC Tru9I 239 TTAA T^TAA 800 TTAA Tth111I 1756 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2365 GACTGTGTC XcmI 1694 CCATGACCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2373 CCAGGCCATCACTGG XhoI 737 CTCGAG C^TCGAG 875 CTCGAG XhoII 681 AGATCT [GA]^GATC[CT] 1023 GGATCC XmnI 582 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 774 GAAGTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I