(Circular) (Six-base) MAPSORT of: pF1KE2548.seq Check: 1466 from: 1 to: 5933 pF1KE2548 5933 bp With 182 enzymes: SgfI * October 24, 2014 10:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1243 1243 Size: 5933 AatII G_ACGT'C Cuts at: 1356 1356 Size: 5933 AccI GT'mk_AC Cuts at: 1742 2132 2971 1742 Size: 390 839 4704 Fragments arranged by size: 4704 839 390 Acc65I G'GTAC_C Cuts at: 2949 2949 Size: 5933 AclI AA'CG_TT Cuts at: 693 5773 693 Size: 5080 853 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 463 1099 2074 2200 2710 2875 3758 4190 4937 Size: 636 975 126 510 165 883 432 747 Cuts at: 4937 5205 463 Size: 268 1191 Fragments arranged by size: 1191 975 883 747 636 510 432 268 165 126 AflIII A'CryG_T Cuts at: 2171 3285 4389 5366 2171 Size: 1114 1104 977 2738 Fragments arranged by size: 2738 1114 1104 977 AgeI A'CCGG_T Cuts at: 4321 4321 Size: 5933 AlwNI CAG_nnn'CTG Cuts at: 1773 2853 3238 4805 1773 Size: 1080 385 1567 2901 Fragments arranged by size: 2901 1567 1080 385 ApaI G_GGCC'C Cuts at: 1321 2708 2867 1321 Size: 1387 159 4387 Fragments arranged by size: 4387 1387 159 ApaLI G'TGCA_C Cuts at: 1750 2149 4703 1750 Size: 399 2554 2980 Fragments arranged by size: 2980 2554 399 ApoI r'AATT_y Cuts at: 2554 2937 5286 5542 2554 Size: 383 2349 256 2945 Fragments arranged by size: 2945 2349 383 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5933 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5933 AvaI C'yCGr_G Cuts at: 845 983 2089 2953 845 Size: 138 1106 864 3825 Fragments arranged by size: 3825 1106 864 138 BamHI G'GATC_C Cuts at: 1131 2958 1131 Size: 1827 4106 BanI G'GyrC_C Cuts at: 1231 2358 2949 3363 3611 3646 1231 Size: 1127 591 414 248 35 3518 Fragments arranged by size: 3518 1127 591 414 248 35 BanII G_rGCy'C Cuts at: 1032 1321 1448 1945 2273 2640 2708 2867 2947 Size: 289 127 497 328 367 68 159 80 Cuts at: 2947 3977 1032 Size: 1030 2988 Fragments arranged by size: 2988 1030 497 367 328 289 159 127 80 68 BbeI G_GCGC'C Cuts at: 3367 3615 3367 Size: 248 5685 BbsI GAAGACnn'nnnn_ Cuts at: 473 634 1976 2878 473 Size: 161 1342 902 3528 Fragments arranged by size: 3528 1342 902 161 BbvCI CC'TCA_GC Cuts at: 385 385 Size: 5933 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2080 2114 2080 Size: 34 5899 BciVI GTATCCnnnnn_n' Cuts at: 2530 2795 3831 4592 5933 2530 Size: 265 1036 761 1341 2530 Fragments arranged by size: 2530 1341 1036 761 265 BclI T'GATC_A Cuts at: 3453 3453 Size: 5933 BglI GCCn_nnn'nGGC Cuts at: 2306 2306 Size: 5933 BglII A'GATC_T Cuts at: 789 3448 5485 789 Size: 2659 2037 1237 Fragments arranged by size: 2659 2037 1237 BlpI GC'TnA_GC Cuts at: 2267 3045 2267 Size: 778 5155 Bme1580I G_kGCm'C Cuts at: 1234 1321 1754 2153 2363 2708 2867 3558 3651 Size: 87 433 399 210 345 159 691 93 Cuts at: 3651 4707 1234 Size: 1056 2460 Fragments arranged by size: 2460 1056 691 433 399 345 210 159 93 87 BmgBI CAC'GTC Cuts at: 546 2565 546 Size: 2019 3914 BmrI ACTGGGnnnn_n' Cuts at: 1861 2332 3145 3226 3561 5705 1861 Size: 471 813 81 335 2144 2089 Fragments arranged by size: 2144 2089 813 471 335 81 BmtI G_CTAG'C Cuts at: 751 751 Size: 5933 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2767 4300 2767 Size: 1533 4400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 679 1284 1368 1434 1542 2121 679 Size: 605 84 66 108 579 4491 Fragments arranged by size: 4491 605 579 108 84 66 Bpu10I CC'TnA_GC Cuts at: 385 1226 385 Size: 841 5092 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 346 409 1596 1947 2768 3109 3988 4480 4778 Size: 63 1187 351 821 341 879 492 298 Cuts at: 4778 346 Size: 1501 Fragments arranged by size: 1501 1187 879 821 492 351 341 298 63 BsaI GGTCTCn'nnnn_ Cuts at: 1915 1923 5637 1915 Size: 8 3714 2211 Fragments arranged by size: 3714 2211 8 BsaAI yAC'GTr Cuts at: 2174 3286 2174 Size: 1112 4821 BsaBI GATnn'nnATC Cuts at: 6 3447 5484 6 Size: 3441 2037 455 Fragments arranged by size: 3441 2037 455 BsaHI Gr'CG_yC Cuts at: 1353 3364 3612 4314 5810 1353 Size: 2011 248 702 1496 1476 Fragments arranged by size: 2011 1496 1476 702 248 BsaWI w'CCGG_w Cuts at: 3122 3643 4321 4595 4742 5153 3122 Size: 521 678 274 147 411 3902 Fragments arranged by size: 3902 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1007 1037 1566 1596 1714 1744 2114 2144 1007 Size: 30 529 30 118 30 370 30 4796 Fragments arranged by size: 4796 529 370 118 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 211 214 217 220 1134 1287 1417 1514 2338 Size: 3 3 3 914 153 130 97 824 Cuts at: 2338 2782 211 Size: 444 3362 Fragments arranged by size: 3362 914 824 444 153 130 97 3 3 3 BseYI C'CCAG_C Cuts at: 537 819 1346 1540 1835 2273 2398 3358 4116 Size: 282 527 194 295 438 125 960 758 Cuts at: 4116 4693 5189 537 Size: 577 496 1281 Fragments arranged by size: 1281 960 758 577 527 496 438 295 282 194 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1668 1668 Size: 5933 BsiEI CG_ry'CG Cuts at: 104 128 3475 3521 4729 104 Size: 24 3347 46 1208 1308 Fragments arranged by size: 3347 1308 1208 46 24 BsiHKAI G_wGCw'C Cuts at: 1448 1754 2153 2250 2570 2947 3725 3915 4707 Size: 306 399 97 320 377 778 190 792 Cuts at: 4707 1448 Size: 2674 Fragments arranged by size: 2674 792 778 399 377 320 306 190 97 BsmI GAATG_Cn' Cuts at: 2160 2160 Size: 5933 Bsp1286I G_dGCh'C Cuts at: 1032 1234 1321 1448 1754 1945 2153 2250 2273 Size: 202 87 127 306 191 208 97 23 Cuts at: 2273 2363 2570 2640 2708 2867 2947 3558 3651 Size: 90 207 70 68 159 80 611 93 Cuts at: 3651 3725 3915 3977 4707 1032 Size: 74 190 62 730 2258 Fragments arranged by size: 2258 730 611 306 208 207 202 191 190 159 127 97 93 90 87 80 74 70 68 62 23 BspEI T'CCGG_A Cuts at: 5153 5153 Size: 5933 BspHI T'CATG_A Cuts at: 5109 5109 Size: 5933 BspMI ACCTGCnnnn'nnnn_ Cuts at: 950 1243 2441 2983 3197 3499 3880 950 Size: 293 1198 542 214 302 381 3003 Fragments arranged by size: 3003 1198 542 381 302 293 214 BsrBI CCG'CTC Cuts at: 50 1371 1522 4225 4279 5763 5928 50 Size: 1321 151 2703 54 1484 165 55 Fragments arranged by size: 2703 1484 1321 165 151 55 54 BsrDI GCAATG_nn' Cuts at: 480 3845 480 Size: 3365 2568 BsrFI r'CCGG_y Cuts at: 127 3931 4112 4321 127 Size: 3804 181 209 1739 Fragments arranged by size: 3804 1739 209 181 BssSI C'ACGA_G Cuts at: 4204 4562 4204 Size: 358 5575 BstAPI GCAn_nnn'nTGC Cuts at: 2157 3310 2157 Size: 1153 4780 BstBI TT'CG_AA Cuts at: 4294 4294 Size: 5933 BstXI CCAn_nnnn'nTGG Cuts at: 2805 2805 Size: 5933 BstYI r'GATC_y Cuts at: 1 789 1131 2958 3425 3448 3783 4029 5041 Size: 788 342 1827 467 23 335 246 1012 Cuts at: 5041 5127 5139 5485 1 Size: 86 12 346 449 Fragments arranged by size: 1827 1012 788 467 449 346 342 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 403 403 Size: 5933 BtgI C'CryG_G Cuts at: 107 139 2345 2505 107 Size: 32 2206 160 3535 Fragments arranged by size: 3535 2206 160 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 404 700 1667 3883 4064 4227 404 Size: 296 967 2216 181 163 2110 Fragments arranged by size: 2216 2110 967 296 181 163 BtsI GCAGTG_nn' Cuts at: 2012 3176 2012 Size: 1164 4769 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2001 2036 2001 Size: 35 5898 DraI TTT'AAA Cuts at: 2932 2932 Size: 5933 DraIII CAC_nnn'GTG Cuts at: 1758 2451 1758 Size: 693 5240 DrdI GACnn_nn'nnGTC Cuts at: 1837 3639 4497 1837 Size: 1802 858 3273 Fragments arranged by size: 3273 1802 858 EaeI y'GGCC_r Cuts at: 125 332 1362 2342 2660 2795 3518 3692 4083 Size: 207 1030 980 318 135 723 174 391 Cuts at: 4083 4110 5455 125 Size: 27 1345 603 Fragments arranged by size: 1345 1030 980 723 603 391 318 207 174 135 27 EagI C'GGCC_G Cuts at: 125 3518 125 Size: 3393 2540 EarI CTCTTCn'nnn_ Cuts at: 235 439 476 503 581 1252 1623 2395 2759 Size: 204 37 27 78 671 371 772 364 Cuts at: 2759 2760 3956 4166 235 Size: 1 1196 210 2002 Fragments arranged by size: 2002 1196 772 671 371 364 210 204 78 37 27 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4141 4451 4597 5743 4141 Size: 310 146 1146 4331 Fragments arranged by size: 4331 1146 310 146 EcoICRI GAG'CTC Cuts at: 1446 2945 1446 Size: 1499 4434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 463 679 1099 1284 1368 1434 1542 2074 2121 Size: 216 420 185 84 66 108 532 47 Cuts at: 2121 2200 2710 2875 3758 4190 4937 5205 463 Size: 79 510 165 883 432 747 268 1191 Fragments arranged by size: 1191 883 747 532 510 432 420 268 216 185 165 108 84 79 66 47 EcoNI CCTnn'n_nnAGG Cuts at: 427 427 Size: 5933 EcoO109I rG'GnC_Cy Cuts at: 1317 1562 1587 3072 1317 Size: 245 25 1485 4178 Fragments arranged by size: 4178 1485 245 25 EcoRI G'AATT_C Cuts at: 2554 2937 2554 Size: 383 5550 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 3952 2278 Size: 1674 4259 FspI TGC'GCA Cuts at: 157 3714 157 Size: 3557 2376 HaeII r_GCGC'y Cuts at: 3367 3615 4637 5616 3367 Size: 248 1022 979 3684 Fragments arranged by size: 3684 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1116 1148 2114 2146 2528 2560 2766 2798 3775 Size: 32 966 32 382 32 206 32 977 Cuts at: 3775 3805 3807 3837 1116 Size: 30 2 30 3212 Fragments arranged by size: 3212 977 966 382 206 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2972 2972 Size: 5933 HindIII A'AGCT_T Cuts at: 1593 1593 Size: 5933 KasI G'GCGC_C Cuts at: 3363 3611 3363 Size: 248 5685 KpnI G_GTAC'C Cuts at: 2953 2953 Size: 5933 MluI A'CGCG_T Cuts at: 5366 5366 Size: 5933 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4604 4788 4604 Size: 184 5749 MscI TGG'CCA Cuts at: 334 2662 3694 334 Size: 2328 1032 2573 Fragments arranged by size: 2573 2328 1032 MslI CAynn'nnrTG Cuts at: 2735 3922 4049 2735 Size: 1187 127 4619 Fragments arranged by size: 4619 1187 127 MspA1I CmG'CkG Cuts at: 141 1430 1603 2098 2277 2427 3044 3358 3718 Size: 1289 173 495 179 150 617 314 360 Cuts at: 3718 4731 4976 141 Size: 1013 245 1098 Fragments arranged by size: 1289 1098 1013 617 495 360 314 245 179 173 150 NaeI GCC'GGC Cuts at: 129 4114 129 Size: 3985 1948 NarI GG'CG_CC Cuts at: 3364 3612 3364 Size: 248 5685 NcoI C'CATG_G Cuts at: 107 2505 107 Size: 2398 3535 NgoMIV G'CCGG_C Cuts at: 127 4112 127 Size: 3985 1948 NheI G'CTAG_C Cuts at: 747 747 Size: 5933 NspI r_CATG'y Cuts at: 799 2986 3486 4393 799 Size: 2187 500 907 2339 Fragments arranged by size: 2339 2187 907 500 PciI A'CATG_T Cuts at: 4389 4389 Size: 5933 PfoI T'CCnGG_A Cuts at: 1134 1558 1134 Size: 424 5509 PmeI GTTT'AAAC Cuts at: 2932 2932 Size: 5933 PmlI CAC'GTG Cuts at: 2174 2174 Size: 5933 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5072 5104 5072 Size: 32 5901 PpuMI rG'GwC_Cy Cuts at: 1562 1587 1562 Size: 25 5908 PsiI TTA'TAA Cuts at: 370 1397 370 Size: 1027 4906 PspOMI G'GGCC_C Cuts at: 1317 2704 2863 1317 Size: 1387 159 4387 Fragments arranged by size: 4387 1387 159 PstI C_TGCA'G Cuts at: 289 781 947 1037 1240 2980 3665 289 Size: 492 166 90 203 1740 685 2557 Fragments arranged by size: 2557 1740 685 492 203 166 90 PvuI CG_AT'CG Cuts at: 104 104 Size: 5933 PvuII CAG'CTG Cuts at: 2098 2277 2427 3358 3718 2098 Size: 179 150 931 360 4313 Fragments arranged by size: 4313 931 360 179 150 RsrII CG'GwC_CG Cuts at: 4128 4128 Size: 5933 SacI G_AGCT'C Cuts at: 1448 2947 1448 Size: 1499 4434 SacII CC_GC'GG Cuts at: 142 142 Size: 5933 SalI G'TCGA_C Cuts at: 2970 2970 Size: 5933 SapI GCTCTTCn'nnn_ Cuts at: 235 1623 3956 4166 235 Size: 1388 2333 210 2002 Fragments arranged by size: 2333 2002 1388 210 SbfI CC_TGCA'GG Cuts at: 1240 2980 1240 Size: 1740 4193 ScaI AGT'ACT Cuts at: 5450 5450 Size: 5933 SexAI A'CCwGG_T Cuts at: 875 875 Size: 5933 SfcI C'TryA_G Cuts at: 33 285 777 943 1033 1063 1236 2976 3661 Size: 252 492 166 90 30 173 1740 685 Cuts at: 3661 4654 4845 33 Size: 993 191 1121 Fragments arranged by size: 1740 1121 993 685 492 252 191 173 166 90 30 SfoI GGC'GCC Cuts at: 3365 3613 3365 Size: 248 5685 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5933 SmaI CCC'GGG Cuts at: 2955 2955 Size: 5933 SmlI C'TyrA_G Cuts at: 361 424 845 983 1611 1926 2783 3088 4003 Size: 63 421 138 628 315 857 305 915 Cuts at: 4003 4495 4757 361 Size: 492 262 1537 Fragments arranged by size: 1537 915 857 628 492 421 315 305 262 138 63 SpeI A'CTAG_T Cuts at: 67 67 Size: 5933 SphI G_CATG'C Cuts at: 799 2986 3486 799 Size: 2187 500 3246 Fragments arranged by size: 3246 2187 500 StyI C'CwwG_G Cuts at: 107 170 335 1112 2106 2505 3067 3421 107 Size: 63 165 777 994 399 562 354 2619 Fragments arranged by size: 2619 994 777 562 399 354 165 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 529 3516 3649 4108 4317 529 Size: 2987 133 459 209 2145 Fragments arranged by size: 2987 2145 459 209 133 TatI w'GTAC_w Cuts at: 556 5448 556 Size: 4892 1041 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1554 1586 1714 1746 5450 5482 1554 Size: 32 128 32 3704 32 2005 Fragments arranged by size: 3704 2005 128 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1867 2476 3730 1867 Size: 609 1254 4070 Fragments arranged by size: 4070 1254 609 XbaI T'CTAG_A Cuts at: 1167 2964 1167 Size: 1797 4136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1809 2488 3428 1809 Size: 679 940 4314 Fragments arranged by size: 4314 940 679 XhoI C'TCGA_G Cuts at: 845 983 845 Size: 138 5795 XmaI C'CCGG_G Cuts at: 2953 2953 Size: 5933 XmnI GAAnn'nnTTC Cuts at: 694 886 694 Size: 192 5741 ZraI GAC'GTC Cuts at: 1354 1354 Size: 5933 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI NsiI PacI PasI PflMI PshAI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI