(Circular) (Six-base) MAPSORT of: pF1KE2567.seq Check: 3345 from: 1 to: 5342 pF1KE2567 5342 bp With 182 enzymes: SgfI * March 23, 2015 09:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 402 402 Size: 5342 AccI GT'mk_AC Cuts at: 2333 2380 2333 Size: 47 5295 Acc65I G'GTAC_C Cuts at: 879 2358 879 Size: 1479 3863 AclI AA'CG_TT Cuts at: 5182 5182 Size: 5342 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 196 1149 1977 2158 3167 3599 4346 4614 196 Size: 953 828 181 1009 432 747 268 924 Fragments arranged by size: 1009 953 924 828 747 432 268 181 AflIII A'CryG_T Cuts at: 1373 2694 3798 4775 1373 Size: 1321 1104 977 1940 Fragments arranged by size: 1940 1321 1104 977 AgeI A'CCGG_T Cuts at: 3730 3730 Size: 5342 AhdI GACnn_n'nnGTC Cuts at: 286 286 Size: 5342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1657 1657 Size: 5342 AlwNI CAG_nnn'CTG Cuts at: 492 2647 4214 492 Size: 2155 1567 1620 Fragments arranged by size: 2155 1620 1567 ApaI G_GGCC'C Cuts at: 1058 1058 Size: 5342 ApaLI G'TGCA_C Cuts at: 4112 4112 Size: 5342 ApoI r'AATT_y Cuts at: 199 2346 4695 4951 199 Size: 2147 2349 256 590 Fragments arranged by size: 2349 2147 590 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5342 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5342 AvaI C'yCGr_G Cuts at: 669 1051 2298 2362 669 Size: 382 1247 64 3649 Fragments arranged by size: 3649 1247 382 64 BamHI G'GATC_C Cuts at: 2367 2367 Size: 5342 BanI G'GyrC_C Cuts at: 125 879 1707 1879 2358 2772 3020 3055 125 Size: 754 828 172 479 414 248 35 2412 Fragments arranged by size: 2412 828 754 479 414 248 172 35 BanII G_rGCy'C Cuts at: 317 585 669 848 974 1058 1132 1177 2356 Size: 268 84 179 126 84 74 45 1179 Cuts at: 2356 3386 317 Size: 1030 2273 Fragments arranged by size: 2273 1179 1030 268 179 126 84 84 74 45 BbeI G_GCGC'C Cuts at: 1711 1883 2776 3024 1711 Size: 172 893 248 4029 Fragments arranged by size: 4029 893 248 172 BbsI GAAGACnn'nnnn_ Cuts at: 271 803 271 Size: 532 4810 BbvCI CC'TCA_GC Cuts at: 1743 1743 Size: 5342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 671 705 671 Size: 34 5308 BciVI GTATCCnnnnn_n' Cuts at: 3240 4001 5342 3240 Size: 761 1341 3240 Fragments arranged by size: 3240 1341 761 BclI T'GATC_A Cuts at: 2862 2862 Size: 5342 BfrBI ATG'CAT Cuts at: 330 330 Size: 5342 BglI GCCn_nnn'nGGC Cuts at: 931 931 Size: 5342 BglII A'GATC_T Cuts at: 176 2857 4894 176 Size: 2681 2037 624 Fragments arranged by size: 2681 2037 624 BlpI GC'TnA_GC Cuts at: 599 2454 599 Size: 1855 3487 Bme1580I G_kGCm'C Cuts at: 518 1058 1211 2967 3060 4116 518 Size: 540 153 1756 93 1056 1744 Fragments arranged by size: 1756 1744 1056 540 153 93 BmgBI CAC'GTC Cuts at: 1160 1560 2082 1160 Size: 400 522 4420 Fragments arranged by size: 4420 522 400 BmrI ACTGGGnnnn_n' Cuts at: 2554 2635 2970 5114 2554 Size: 81 335 2144 2782 Fragments arranged by size: 2782 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 701 2262 3709 701 Size: 1561 1447 2334 Fragments arranged by size: 2334 1561 1447 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 124 403 469 599 2220 2253 124 Size: 279 66 130 1621 33 3213 Fragments arranged by size: 3213 1621 279 130 66 33 Bpu10I CC'TnA_GC Cuts at: 1743 1743 Size: 5342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 217 2518 3397 3889 4187 217 Size: 2301 879 492 298 1372 Fragments arranged by size: 2301 1372 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1190 5046 1190 Size: 3856 1486 BsaAI yAC'GTr Cuts at: 1374 2695 1374 Size: 1321 4021 BsaBI GATnn'nnATC Cuts at: 6 812 2856 4893 6 Size: 806 2044 2037 455 Fragments arranged by size: 2044 2037 806 455 BsaHI Gr'CG_yC Cuts at: 1582 1708 1781 1880 2206 2773 3021 3723 5219 Size: 126 73 99 326 567 248 702 1496 Cuts at: 5219 1582 Size: 1705 Fragments arranged by size: 1705 1496 702 567 326 248 126 99 73 BsaWI w'CCGG_w Cuts at: 2531 3052 3730 4004 4151 4562 2531 Size: 521 678 274 147 411 3311 Fragments arranged by size: 3311 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1255 1285 1981 2011 1255 Size: 30 696 30 4586 Fragments arranged by size: 4586 696 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 727 730 1014 1140 727 Size: 3 284 126 4929 Fragments arranged by size: 4929 284 126 3 BseYI C'CCAG_C Cuts at: 531 850 1057 1210 1336 1592 1749 1952 2767 Size: 319 207 153 126 256 157 203 815 Cuts at: 2767 3525 4102 4598 531 Size: 758 577 496 1275 Fragments arranged by size: 1275 815 758 577 496 319 256 207 203 157 153 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1444 2062 2092 1444 Size: 618 30 4694 Fragments arranged by size: 4694 618 30 BsiEI CG_ry'CG Cuts at: 104 186 724 2048 2884 2930 4138 104 Size: 82 538 1324 836 46 1208 1308 Fragments arranged by size: 1324 1308 1208 836 538 82 46 BsiHKAI G_wGCw'C Cuts at: 419 974 2173 2356 3134 3324 4116 419 Size: 555 1199 183 778 190 792 1645 Fragments arranged by size: 1645 1199 792 778 555 190 183 BsiWI C'GTAC_G Cuts at: 308 2210 308 Size: 1902 3440 BsmI GAATG_Cn' Cuts at: 890 890 Size: 5342 BsmBI CGTCTCn'nnnn_ Cuts at: 1326 1326 Size: 5342 Bsp1286I G_dGCh'C Cuts at: 317 419 518 585 669 848 974 1058 1132 Size: 102 99 67 84 179 126 84 74 Cuts at: 1132 1177 1211 2173 2356 2967 3060 3134 3324 Size: 45 34 962 183 611 93 74 190 Cuts at: 3324 3386 4116 317 Size: 62 730 1543 Fragments arranged by size: 1543 962 730 611 190 183 179 126 102 99 93 84 84 74 74 67 62 45 34 BspEI T'CCGG_A Cuts at: 4562 4562 Size: 5342 BspHI T'CATG_A Cuts at: 4518 4518 Size: 5342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 402 432 1841 1967 2195 2392 2606 2908 3289 Size: 30 1409 126 228 197 214 302 381 Cuts at: 3289 402 Size: 2455 Fragments arranged by size: 2455 1409 381 302 228 214 197 126 30 BsrBI CCG'CTC Cuts at: 50 138 362 3634 3688 5172 5337 50 Size: 88 224 3272 54 1484 165 55 Fragments arranged by size: 3272 1484 224 165 88 55 54 BsrDI GCAATG_nn' Cuts at: 3254 3254 Size: 5342 BsrFI r'CCGG_y Cuts at: 1356 1398 3340 3521 3730 1356 Size: 42 1942 181 209 2968 Fragments arranged by size: 2968 1942 209 181 42 BsrGI T'GTAC_A Cuts at: 1523 1523 Size: 5342 BssSI C'ACGA_G Cuts at: 706 2116 3613 3971 706 Size: 1410 1497 358 2077 Fragments arranged by size: 2077 1497 1410 358 BstAPI GCAn_nnn'nTGC Cuts at: 414 2719 414 Size: 2305 3037 BstBI TT'CG_AA Cuts at: 3703 3703 Size: 5342 BstEII G'GTnAC_C Cuts at: 444 1494 444 Size: 1050 4292 BstYI r'GATC_y Cuts at: 1 176 2072 2367 2834 2857 3192 3438 4450 Size: 175 1896 295 467 23 335 246 1012 Cuts at: 4450 4536 4548 4894 1 Size: 86 12 346 449 Fragments arranged by size: 1896 1012 467 449 346 335 295 246 175 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1123 1123 Size: 5342 BtgI C'CryG_G Cuts at: 449 1106 1441 1473 1586 449 Size: 657 335 32 113 4205 Fragments arranged by size: 4205 657 335 113 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1823 3292 3473 3636 1823 Size: 1469 181 163 3529 Fragments arranged by size: 3529 1469 181 163 BtsI GCAGTG_nn' Cuts at: 558 2019 2585 558 Size: 1461 566 3315 Fragments arranged by size: 3315 1461 566 DraI TTT'AAA Cuts at: 2341 2341 Size: 5342 DrdI GACnn_nn'nnGTC Cuts at: 3048 3906 3048 Size: 858 4484 EaeI y'GGCC_r Cuts at: 147 254 1064 1650 1766 2927 3101 3492 3519 Size: 107 810 586 116 1161 174 391 27 Cuts at: 3519 4864 147 Size: 1345 625 Fragments arranged by size: 1345 1161 810 625 586 391 174 116 107 27 EagI C'GGCC_G Cuts at: 2927 2927 Size: 5342 EarI CTCTTCn'nnn_ Cuts at: 1329 3365 3575 1329 Size: 2036 210 3096 Fragments arranged by size: 3096 2036 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 355 3550 3860 4006 5152 355 Size: 3195 310 146 1146 545 Fragments arranged by size: 3195 1146 545 310 146 EcoICRI GAG'CTC Cuts at: 972 2354 972 Size: 1382 3960 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 124 196 403 469 599 1149 1977 2158 2220 Size: 72 207 66 130 550 828 181 62 Cuts at: 2220 2253 3167 3599 4346 4614 124 Size: 33 914 432 747 268 852 Fragments arranged by size: 914 852 828 747 550 432 268 207 181 130 72 66 62 33 EcoNI CCTnn'n_nnAGG Cuts at: 394 1963 394 Size: 1569 3773 EcoO109I rG'GnC_Cy Cuts at: 778 906 1698 1830 1929 1956 2481 778 Size: 128 792 132 99 27 525 3639 Fragments arranged by size: 3639 792 525 132 128 99 27 EcoRI G'AATT_C Cuts at: 199 2346 199 Size: 2147 3195 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1809 3361 1809 Size: 1552 3790 FspI TGC'GCA Cuts at: 3123 3123 Size: 5342 HaeII r_GCGC'y Cuts at: 1711 1883 2776 3024 4046 5025 1711 Size: 172 893 248 1022 979 2028 Fragments arranged by size: 2028 1022 979 893 248 172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 272 304 823 855 1981 2013 3184 3214 3216 Size: 32 519 32 1126 32 1171 30 2 Cuts at: 3216 3246 272 Size: 30 2368 Fragments arranged by size: 2368 1171 1126 519 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2381 2381 Size: 5342 KasI G'GCGC_C Cuts at: 1707 1879 2772 3020 1707 Size: 172 893 248 4029 Fragments arranged by size: 4029 893 248 172 KpnI G_GTAC'C Cuts at: 883 2362 883 Size: 1479 3863 MluI A'CGCG_T Cuts at: 4775 4775 Size: 5342 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2028 4013 4197 2028 Size: 1985 184 3173 Fragments arranged by size: 3173 1985 184 MscI TGG'CCA Cuts at: 256 3103 256 Size: 2847 2495 MslI CAynn'nnrTG Cuts at: 325 3331 3458 325 Size: 3006 127 2209 Fragments arranged by size: 3006 2209 127 MspA1I CmG'CkG Cuts at: 492 603 954 1443 1458 1505 1886 1977 2106 Size: 111 351 489 15 47 381 91 129 Cuts at: 2106 2453 2767 3127 4140 4385 492 Size: 347 314 360 1013 245 1449 Fragments arranged by size: 1449 1013 489 381 360 351 347 314 245 129 111 91 47 15 NaeI GCC'GGC Cuts at: 1358 3523 1358 Size: 2165 3177 NarI GG'CG_CC Cuts at: 1708 1880 2773 3021 1708 Size: 172 893 248 4029 Fragments arranged by size: 4029 893 248 172 NcoI C'CATG_G Cuts at: 1473 1473 Size: 5342 NgoMIV G'CCGG_C Cuts at: 1356 3521 1356 Size: 2165 3177 NsiI A_TGCA'T Cuts at: 332 332 Size: 5342 NspI r_CATG'y Cuts at: 1522 2395 2895 3802 1522 Size: 873 500 907 3062 Fragments arranged by size: 3062 907 873 500 PasI CC'CwG_GG Cuts at: 117 1345 2013 117 Size: 1228 668 3446 Fragments arranged by size: 3446 1228 668 PciI A'CATG_T Cuts at: 3798 3798 Size: 5342 PmeI GTTT'AAAC Cuts at: 2341 2341 Size: 5342 PmlI CAC'GTG Cuts at: 1374 1374 Size: 5342 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 4481 Size: 32 5310 PpuMI rG'GwC_Cy Cuts at: 778 906 1698 1830 1956 778 Size: 128 792 132 126 4164 Fragments arranged by size: 4164 792 132 128 126 PshAI GACnn'nnGTC Cuts at: 1849 1849 Size: 5342 PspOMI G'GGCC_C Cuts at: 1054 1054 Size: 5342 PstI C_TGCA'G Cuts at: 1807 1838 1982 2389 3074 1807 Size: 31 144 407 685 4075 Fragments arranged by size: 4075 685 407 144 31 PvuI CG_AT'CG Cuts at: 104 104 Size: 5342 PvuII CAG'CTG Cuts at: 603 1458 1886 1977 2106 2767 3127 603 Size: 855 428 91 129 661 360 2818 Fragments arranged by size: 2818 855 661 428 360 129 91 RsrII CG'GwC_CG Cuts at: 3537 3537 Size: 5342 SacI G_AGCT'C Cuts at: 974 2356 974 Size: 1382 3960 SacII CC_GC'GG Cuts at: 1444 1444 Size: 5342 SalI G'TCGA_C Cuts at: 2379 2379 Size: 5342 SapI GCTCTTCn'nnn_ Cuts at: 3365 3575 3365 Size: 210 5132 SbfI CC_TGCA'GG Cuts at: 2389 2389 Size: 5342 ScaI AGT'ACT Cuts at: 4859 4859 Size: 5342 SexAI A'CCwGG_T Cuts at: 908 908 Size: 5342 SfcI C'TryA_G Cuts at: 33 1803 1834 1978 2385 3070 4063 4254 33 Size: 1770 31 144 407 685 993 191 1121 Fragments arranged by size: 1770 1121 993 685 407 191 144 31 SfoI GGC'GCC Cuts at: 1709 1881 2774 3022 1709 Size: 172 893 248 4029 Fragments arranged by size: 4029 893 248 172 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5342 SmaI CCC'GGG Cuts at: 1053 2364 1053 Size: 1311 4031 SmlI C'TyrA_G Cuts at: 232 2497 3412 3904 4166 232 Size: 2265 915 492 262 1408 Fragments arranged by size: 2265 1408 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5342 SphI G_CATG'C Cuts at: 2395 2895 2395 Size: 500 4842 StyI C'CwwG_G Cuts at: 929 942 965 1473 1784 2234 2476 2830 929 Size: 13 23 508 311 450 242 354 3441 Fragments arranged by size: 3441 508 450 354 311 242 23 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 738 2925 3058 3517 3726 738 Size: 2187 133 459 209 2354 Fragments arranged by size: 2354 2187 459 209 133 TatI w'GTAC_w Cuts at: 1523 4857 1523 Size: 3334 2008 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4859 4891 4859 Size: 32 5310 Tth111I GACn'n_nGTC Cuts at: 1114 1140 3139 1114 Size: 26 1999 3317 Fragments arranged by size: 3317 1999 26 XbaI T'CTAG_A Cuts at: 2373 2373 Size: 5342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1584 1698 2837 1584 Size: 114 1139 4089 Fragments arranged by size: 4089 1139 114 XmaI C'CCGG_G Cuts at: 1051 2362 1051 Size: 1311 4031 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AleI AloI AscI AvrII BaeI BmtI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PflMI PfoI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI