Restriction map for pF1KE3148
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfaI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr13I      ClaI        CpoI        
DraI        DraII       DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeIII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MluI        MluNI       
MroI        MscI        MspA1I      NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI           28  GTCTAC               GT^[AC][GT]AC
AcyI           18  GACGCC               G[GA]^CG[CT]C
AflII         432  CTTAAG               C^TTAAG
AgeI          636  ACCGGT               A^CCGGT
AspI          154  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI         442  GGATAC               GTATCC(6/5)
BfrI          432  CTTAAG               C^TTAAG
BlpI           37  GCTCAGC              GC^T[ACGT]AGC
BpmI          175  CTGGAG               CTGGAG(16/14)
Bpu1102I       37  GCTCAGC              GC^T[ACGT]AGC
BsaHI          18  GACGCC               G[GA]^CG[CT]C
BsaI          916  GAGACC               GGTCTC(1/5)
BsaJI         701  CCATGG               C^C[ACGT][ACGT]GG
BspMI         228  GCAGGT               ACCTGC(4/8)
BsrDI         938  CATTGC               GCAATG(2/0)
BsrFI         636  ACCGGT               [GA]^CCGG[CT]
BstXI         657  CCAGCTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII          37  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        636  ACCGGT               [GA]^CCGG[CT]
DraIII        837  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          701  CCATGG               C^C[GA][CT]GG
EarI          373  GAAGAG               CTCTTC(1/4)
EcoT14I       701  CCATGG               C^C[AT][AT]GG
HgaI           18  GACGC                GACGC(5/10)
Hin1I          18  GACGCC               G[GA]^CG[CT]C
Ksp632I       373  GAAGAG               CTCTTC(1/4)
MnlI          225  GAGG                 CCTC(7/6)
MseI          433  TTAA                 T^TAA
NcoI          701  CCATGG               C^CATGG
PflFI         154  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         636  ACCGGT               A^CCGGT
SacI          355  GAGCTC               GAGCT^C
SapI          373  GAAGAGC              GCTCTTC(1/4)
SgrAI         635  CACCGGTG             C[GA]^CCGG[CT]G
SspI          906  AATATT               AAT^ATT
StyI          701  CCATGG               C^C[AT][AT]GG
Tru9I         433  TTAA                 T^TAA
Tth111I       154  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccII          49  CGCG                 CG^CG
               64  CGCG                
AlwNI         200  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              583  CAGATGCTG           
AspHI         355  GAGCTC               G[AT]GC[AT]^C
              817  GTGCTC              
BanI          142  GGTGCC               G^G[CT][GA]CC
              697  GGCACC              
BanII          35  GGGCTC               G[GA]GC[CT]^C
              355  GAGCTC              
BsaWI         636  ACCGGT               [AT]^CCGG[AT]
              919  ACCGGA              
BsiHKAI       355  GAGCTC               G[AT]GC[AT]^C
              817  GTGCTC              
BssKI         174  CCTGG                ^CC[ACGT]GG
              485  CCTGG               
BstF5I        333  GGATG                GGATG(2/0)
              865  GGATG               
BstNI         174  CCTGG                CC^[AT]GG
              485  CCTGG               
BstUI          49  CGCG                 CG^CG
               64  CGCG                
BstYI         488  GGATCT               [GA]^GATC[CT]
             1079  AGATCC              
Cac8I          50  GCGAGC               GC[ACGT]^[ACGT]GC
              588  GCTGGC              
EcoRII        174  CCTGG                ^CC[AT]GG
              485  CCTGG               
FokI          333  GGATG                GGATG(9/13)
              865  GGATG               
HaeII         377  AGCGCC               [GA]GCGC^[CT]
             1055  GGCGCT              
HapII         637  CCGG                 C^CGG
              920  CCGG                
HincII         15  GTTGAC               GT[CT]^[GA]AC
              496  GTCAAC              
HindII         15  GTTGAC               GT[CT]^[GA]AC
              496  GTCAAC              
HpaII         637  CCGG                 C^CGG
              920  CCGG                
MfeI          617  CAATTG               C^AATTG
              758  CAATTG              
MflI          488  GGATCT               [GA]^GATC[CT]
             1079  AGATCC              
MslI          798  CATTGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              860  CACTGGGATG          
MspI          637  CCGG                 C^CGG
              920  CCGG                
MunI          617  CAATTG               C^AATTG
              758  CAATTG              
MvaI          174  CCTGG                CC^[AT]GG
              485  CCTGG               
MvnI           49  CGCG                 CG^CG
               64  CGCG                
NspI           24  GCATGT               [GA]CATG^[CT]
              785  ACATGC              
PflMI         552  CCATACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              582  CCAGATGCTGG         
ScrFI         174  CCTGG                CC^[ACGT]GG
              485  CCTGG               
SmlI          131  CTCAAG               C^T[CT][GA]AG
              432  CTTAAG              
Van91I        552  CCATACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              582  CCAGATGCTGG         
XhoII         488  GGATCT               [GA]^GATC[CT]
             1079  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BmyI        BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        CfoI        DdeI        
DpnII       Fnu4HI      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      SfaNI       TfiI        
TseI        Tsp45I      Tsp509I     TspRI       
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