Restriction map for
pF1KE2712
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI HphI KpnI Ksp632I
MaeI MaeII MaeIII MboII MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 175 GGCGCC G[GA]^CG[CT]C
AlwI 482 GGATC GGATC(4/5)
AlwNI 447 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 524 CCTCAGG CC^T[ACGT]AGG
Asp700I 369 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 175 GGCGCC GGCGC^C
BcgI 151 GCAAGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 514 GCTCAGC GC^T[ACGT]AGC
BmyI 18 GGGCAC G[AGT]GC[ACT]^C
BpmI 457 CTCCAG CTGGAG(16/14)
Bpu1102I 514 GCTCAGC GC^T[ACGT]AGC
BsaBI 477 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 175 GGCGCC G[GA]^CG[CT]C
BseRI 170 GAGGAG GAGGAG(10/8)
BsmAI 455 GTCTC GTCTC(1/5)
BsmFI 131 GTCCC GGGAC(10/14)
BsmI 65 GCATTC GAATGC(1/-1)
Bsp1286I 18 GGGCAC G[AGT]GC[ACT]^C
BsrDI 86 GCAATG GCAATG(2/0)
Bsu36I 524 CCTCAGG CC^T[ACGT]AGG
CelII 514 GCTCAGC GC^T[ACGT]AGC
DpnII 483 GATC ^GATC
DraIII 486 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 524 CCTCAGG CC^T[ACGT]AGG
EcoT14I 296 CCATGG C^C[AT][AT]GG
HaeII 175 GGCGCC [GA]GCGC^[CT]
Hin1I 175 GGCGCC G[GA]^CG[CT]C
KasI 175 GGCGCC G^GCGCC
KspI 181 CCGCGG CCGC^GG
MamI 477 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 483 GATC ^GATC
MspA1I 181 CCGCGG C[AC]G^C[GT]G
NarI 175 GGCGCC GG^CGCC
NcoI 296 CCATGG C^CATGG
NdeII 483 GATC ^GATC
NruI 287 TCGCGA TCG^CGA
PpuMI 129 AGGTCCC [GA]G^G[AT]CC[CT]
SacII 181 CCGCGG CCGC^GG
Sau3AI 483 GATC ^GATC
SfiI 49 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 175 GGCGCC GGC^GCC
SmaI 134 CCCGGG CCC^GGG
SmlI 262 CTTGAG C^T[CT][GA]AG
StyI 296 CCATGG C^C[AT][AT]GG
TaqI 31 TCGA T^CGA
TthHB8I 31 TCGA T^CGA
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
XmnI 369 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AvaI 134 CCCGGG C^[CT]CG[GA]G
159 CTCGGG
AvaII 130 GGTCC G^G[AT]CC
187 GGTCC
BanI 112 GGTGCC G^G[CT][GA]CC
175 GGCGCC
BglI 50 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
77 GCCCAGGGGGC
BsoBI 134 CCCGGG C^[CT]CG[GA]G
159 CTCGGG
BstF5I 220 GGATG GGATG(2/0)
333 GGATG
DdeI 515 CTCAG C^T[ACGT]AG
525 CTCAG
EcoNI 324 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
520 CCTCCCTCAGG
FokI 220 GGATG GGATG(9/13)
333 GGATG
TspRI 378 CACTG CA[GC]TG[ACGT][ACGT]^
490 CAGTG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
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AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PstI
Sau96I ScrFI SfaNI SfcI TseI