Restriction map for
pF1KE2712
(Circular) (Six-base) MAPSORT of: pF1KE2712.seq Check: 2100 from: 1 to: 3653
pF1KE2712 3653 bp
With 182 enzymes: SgfI *
January 5, 2018 10:00 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 496 1478 1910 2657 2925 299
Size: 197 982 432 747 268 1027
Fragments arranged by size:
1027 982 747 432 268 197
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 560 958 2525 560
Size: 398 1567 1688
Fragments arranged by size:
1688 1567 398
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 242 267 673 242
Size: 25 406 3222
Fragments arranged by size:
3222 406 25
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 220 283 669 1083 1331 1366 220
Size: 63 386 414 248 35 2507
Fragments arranged by size:
2507 414 386 248 63 35
BanII G_rGCy'C
Cuts at: 667 1697 667
Size: 1030 2623
BbeI G_GCGC'C
Cuts at: 287 1087 1335 287
Size: 800 248 2605
Fragments arranged by size:
2605 800 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 248 282 90
Size: 34 124 34 3461
Fragments arranged by size:
3461 124 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglI GCCn_nnn'nGGC
Cuts at: 112 164 191 112
Size: 52 27 3574
Fragments arranged by size:
3574 52 27
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 623 765 623
Size: 142 3511
Bme1580I G_kGCm'C
Cuts at: 130 1278 1371 2427 130
Size: 1148 93 1056 1356
Fragments arranged by size:
1356 1148 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 550
Size: 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 829 1708 2200 2498 391
Size: 438 879 492 298 1546
Fragments arranged by size:
1546 879 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 589 1167 3204 6
Size: 583 578 2037 455
Fragments arranged by size:
2037 583 578 455
BsaHI Gr'CG_yC
Cuts at: 284 1084 1332 2034 3530 284
Size: 800 248 702 1496 407
Fragments arranged by size:
1496 800 702 407 248
BsaWI w'CCGG_w
Cuts at: 842 1363 2041 2315 2462 2873 842
Size: 521 678 274 147 411 1622
Fragments arranged by size:
1622 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 293 293
Size: 3653
BseYI C'CCAG_C
Cuts at: 1078 1836 2413 2909 1078
Size: 758 577 496 1822
Fragments arranged by size:
1822 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
BsmI GAATG_Cn'
Cuts at: 173 173
Size: 3653
Bsp1286I G_dGCh'C
Cuts at: 130 667 1278 1371 1445 1635 1697 2427 130
Size: 537 611 93 74 190 62 730 1356
Fragments arranged by size:
1356 730 611 537 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 201 1565 201
Size: 1364 2289
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 633 633
Size: 3653
BtgI C'CryG_G
Cuts at: 224 289 404 224
Size: 65 115 3473
Fragments arranged by size:
3473 115 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 161 1603 1784 1947 161
Size: 1442 181 163 1867
Fragments arranged by size:
1867 1442 181 163
BtsI GCAGTG_nn'
Cuts at: 604 896 604
Size: 292 3361
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DraIII CAC_nnn'GTG
Cuts at: 599 599
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 1238 1412 1803 1830 3175 1238
Size: 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 496 550 1478 1910 2657 2925 299
Size: 197 54 928 432 747 268 1027
Fragments arranged by size:
1027 928 747 432 268 197 54
EcoNI CCTnn'n_nnAGG
Cuts at: 436 632 436
Size: 196 3457
EcoO109I rG'GnC_Cy
Cuts at: 166 238 263 792 166
Size: 72 25 529 3027
Fragments arranged by size:
3027 529 72 25
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 287 1087 1335 2357 3336 287
Size: 800 248 1022 979 604
Fragments arranged by size:
1022 979 800 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 283 1083 1331 283
Size: 800 248 2605
Fragments arranged by size:
2605 800 248
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 537 2324 2508 537
Size: 1787 184 1682
Fragments arranged by size:
1787 1682 184
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 291 764 1078 1438 2451 2696 291
Size: 473 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 473 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 284 1084 1332 284
Size: 800 248 2605
Fragments arranged by size:
2605 800 248
NcoI C'CATG_G
Cuts at: 404 404
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NruI TCG'CGA
Cuts at: 397 397
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PasI CC'CwG_GG
Cuts at: 187 187
Size: 3653
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PpuMI rG'GwC_Cy
Cuts at: 238 238
Size: 3653
PstI C_TGCA'G
Cuts at: 337 528 545 700 1385 337
Size: 191 17 155 685 2605
Fragments arranged by size:
2605 685 191 155 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SacII CC_GC'GG
Cuts at: 292 292
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 333 524 541 696 1381 2374 2565 33
Size: 300 191 17 155 685 993 191 1121
Fragments arranged by size:
1121 993 685 300 191 191 155 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 164 164
Size: 3653
SfoI GGC'GCC
Cuts at: 285 1085 1333 285
Size: 800 248 2605
Fragments arranged by size:
2605 800 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 244 675 244
Size: 431 3222
SmlI C'TyrA_G
Cuts at: 370 808 1723 2215 2477 370
Size: 438 915 492 262 1546
Fragments arranged by size:
1546 915 492 438 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 404 787 1141 404
Size: 383 354 2916
Fragments arranged by size:
2916 383 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 417 1236 1369 1828 2037 417
Size: 819 133 459 209 2033
Fragments arranged by size:
2033 819 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 242 673 242
Size: 431 3222
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI