Restriction map for
pF1KE2712
(Linear) (Six-base) MAPSORT of: KIEE2712.seq Check: 6866 from: 1 to: 540
KIEE2712.orf
With 182 enzymes: SgfI *
January 5, 2018 10:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 388 540
Size: 191 197 152
Fragments arranged by size:
197 191 152
AlwNI CAG_nnn'CTG
Cuts at: 0 452 540
Size: 452 88
AvaI C'yCGr_G
Cuts at: 0 134 159 540
Size: 134 25 381
Fragments arranged by size:
381 134 25
BanI G'GyrC_C
Cuts at: 0 112 175 540
Size: 112 63 365
Fragments arranged by size:
365 112 63
BbeI G_GCGC'C
Cuts at: 0 179 540
Size: 179 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 140 174 540
Size: 140 34 366
Fragments arranged by size:
366 140 34
BglI GCCn_nnn'nGGC
Cuts at: 0 56 83 540
Size: 56 27 457
Fragments arranged by size:
457 56 27
BlpI GC'TnA_GC
Cuts at: 0 515 540
Size: 515 25
Bme1580I G_kGCm'C
Cuts at: 0 22 540
Size: 22 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 540
Size: 442 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 540
Size: 283 257
BsaBI GATnn'nnATC
Cuts at: 0 481 540
Size: 481 59
BsaHI Gr'CG_yC
Cuts at: 0 176 540
Size: 176 364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 185 540
Size: 185 355
BsmI GAATG_Cn'
Cuts at: 0 65 540
Size: 65 475
Bsp1286I G_dGCh'C
Cuts at: 0 22 540
Size: 22 518
BsrDI GCAATG_nn'
Cuts at: 0 93 540
Size: 93 447
Bsu36I CC'TnA_GG
Cuts at: 0 525 540
Size: 525 15
BtgI C'CryG_G
Cuts at: 0 116 181 296 540
Size: 116 65 115 244
Fragments arranged by size:
244 116 115 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 53 540
Size: 53 487
BtsI GCAGTG_nn'
Cuts at: 0 496 540
Size: 496 44
DraIII CAC_nnn'GTG
Cuts at: 0 491 540
Size: 491 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 388 442 540
Size: 191 197 54 98
Fragments arranged by size:
197 191 98 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 524 540
Size: 328 196 16
Fragments arranged by size:
328 196 16
EcoO109I rG'GnC_Cy
Cuts at: 0 58 130 155 540
Size: 58 72 25 385
Fragments arranged by size:
385 72 58 25
HaeII r_GCGC'y
Cuts at: 0 179 540
Size: 179 361
KasI G'GCGC_C
Cuts at: 0 175 540
Size: 175 365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 540
Size: 429 111
MspA1I CmG'CkG
Cuts at: 0 183 540
Size: 183 357
NarI GG'CG_CC
Cuts at: 0 176 540
Size: 176 364
NcoI C'CATG_G
Cuts at: 0 296 540
Size: 296 244
NruI TCG'CGA
Cuts at: 0 289 540
Size: 289 251
PasI CC'CwG_GG
Cuts at: 0 79 540
Size: 79 461
PpuMI rG'GwC_Cy
Cuts at: 0 130 540
Size: 130 410
PstI C_TGCA'G
Cuts at: 0 229 420 437 540
Size: 229 191 17 103
Fragments arranged by size:
229 191 103 17
SacII CC_GC'GG
Cuts at: 0 184 540
Size: 184 356
SfcI C'TryA_G
Cuts at: 0 225 416 433 540
Size: 225 191 17 107
Fragments arranged by size:
225 191 107 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 56 540
Size: 56 484
SfoI GGC'GCC
Cuts at: 0 177 540
Size: 177 363
SmaI CCC'GGG
Cuts at: 0 136 540
Size: 136 404
SmlI C'TyrA_G
Cuts at: 0 262 540
Size: 262 278
StyI C'CwwG_G
Cuts at: 0 296 540
Size: 296 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 309 540
Size: 309 231
XmaI C'CCGG_G
Cuts at: 0 134 540
Size: 134 406
XmnI GAAnn'nnTTC
Cuts at: 0 373 540
Size: 373 167
Enzymes that do cut:
AcuI AlwNI AvaI BanI BbeI BcgI BglI BlpI
Bme1580I BpmI BpuEI BsaBI BsaHI BseRI BsmI Bsp1286I
BsrDI Bsu36I BtgI BtgZI BtsI DraIII Eco57MI EcoNI
EcoO109I HaeII KasI MmeI MspA1I NarI NcoI NruI
PasI PpuMI PstI SacII SfcI SfiI SfoI SmaI
SmlI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI
BbvCI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI MslI NaeI NdeI NgoMIV NheI NotI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI ZraI