Restriction map for pF1KE2356
##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AclI        AflII       AhdI        Aor51HI     AscI        
AseI        AsnI        AspEI       AvrII       BciVI       
BfrI        BlnI        BsiWI       BspLU11I    BssHII      
Bst1107I    BstBI       BstZ17I     CpoI        DraI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoRI       EcoT22I     FseI        HindIII     
HpaI        Ksp632I     KspI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        4252  GACGTC               GACGT^C
Acc65I       5098  GGTACC               G^GTACC
AccI          934  GTCTAC               GT^[AC][GT]AC
AccIII       7792  TCCGGA               T^CCGGA
AcsI         3709  AAATTC               [GA]^AATT[CT]
AgeI         7558  ACCGGT               A^CCGGT
ApoI         3709  AAATTC               [GA]^AATT[CT]
Asp718I      5098  GGTACC               G^GTACC
AviII        6050  TGCGCA               TGC^GCA
BclI         4994  TGATCA               T^GATCA
BglII        4730  AGATCT               A^GATCT
BlpI          228  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      228  GCTCAGC              GC^T[ACGT]AGC
BsaBI        5505  GATGGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        7792  TCCGGA               T^CCGGA
Bsp1407I     8072  TGTACA               T^GTACA
BspDI        7432  ATCGAT               AT^CGAT
BspEI        7792  TCCGGA               T^CCGGA
BspHI        4608  TCATGA               T^CATGA
BsrGI        8072  TGTACA               T^GTACA
CelII         228  GCTCAGC              GC^T[ACGT]AGC
ClaI         7432  ATCGAT               AT^CGAT
EcoRV        3115  GATATC               GAT^ATC
FbaI         4994  TGATCA               T^GATCA
FspI         6050  TGCGCA               TGC^GCA
KpnI         5098  GGTACC               GGTAC^C
MamI         5505  GATGGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         7792  TCCGGA               T^CCGGA
PinAI        7558  ACCGGT               A^CCGGT
RcaI         4608  TCATGA               T^CATGA
SbfI         4428  CCTGCAGG             CCTGCA^GG
ScaI         1607  AGTACT               AGT^ACT
Sse8387I     4428  CCTGCAGG             CCTGCA^GG
SspBI        8072  TGTACA               T^GTACA
Tsp509I      3710  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AflIII        296  ACGTGT               A^C[GA][CT]GT
             5891  ACGTGT              
AocI          709  CCTGAGG              CC^T[ACGT]AGG
             7666  CCTGAGG             
Asp700I       117  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             7959  GAACTCCTTC          
AspI         2845  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7842  GACCAAGTC           
BamHI         503  GGATCC               G^GATCC
             6694  GGATCC              
BbsI         2325  GAAGAC               GAAGAC(2/6)
             5421  GAAGAC              
BfaI         4153  CTAG                 C^TAG
             7061  CTAG                
BpuAI        2325  GAAGAC               GAAGAC(2/6)
             5421  GAAGAC              
BsaWI        7558  ACCGGT               [AT]^CCGG[AT]
             7792  TCCGGA              
BsiEI        3740  CGGTCG               CG[GA][CT]^CG
             6860  CGGTCG              
Bsu36I        709  CCTGAGG              CC^T[ACGT]AGG
             7666  CCTGAGG             
Eco81I        709  CCTGAGG              CC^T[ACGT]AGG
             7666  CCTGAGG             
EcoNI          81  CCTGGCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3795  CCTGCGGGAGG         
MaeI         4153  CTAG                 C^TAG
             7061  CTAG                
MseI         2207  TTAA                 T^TAA
             2921  TTAA                
PflFI        2845  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7842  GACCAAGTC           
PshAI        2128  GACGGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4402  GACTGCAGTC          
Tru9I        2207  TTAA                 T^TAA
             2921  TTAA                
Tth111I      2845  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7842  GACCAAGTC           
XmnI          117  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             7959  GAACTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaI        ApaLI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BcnI        BglI        BmyI        BpmI        
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BssSI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PaeR7I      PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XhoI        
XhoII       XmaCI       XmaI        
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