(Circular) (Six-base) MAPSORT of: pF1KE2454.seq Check: 8463 from: 1 to: 5651 pF1KE2454 5651 bp With 182 enzymes: SgfI * February 8, 2013 18:16 .. AccI GT'mk_AC Cuts at: 2689 2689 Size: 5651 Acc65I G'GTAC_C Cuts at: 2667 2667 Size: 5651 AclI AA'CG_TT Cuts at: 5491 5491 Size: 5651 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1741 1897 1967 3476 3908 4655 4923 1741 Size: 156 70 1509 432 747 268 2469 Fragments arranged by size: 2469 1509 747 432 268 156 70 AflIII A'CryG_T Cuts at: 1562 3003 4107 5084 1562 Size: 1441 1104 977 2129 Fragments arranged by size: 2129 1441 1104 977 AgeI A'CCGG_T Cuts at: 4039 4039 Size: 5651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 328 328 Size: 5651 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1033 1065 1673 1705 1033 Size: 32 608 32 4979 Fragments arranged by size: 4979 608 32 32 AlwNI CAG_nnn'CTG Cuts at: 1864 2956 4523 1864 Size: 1092 1567 2992 Fragments arranged by size: 2992 1567 1092 ApaI G_GGCC'C Cuts at: 636 636 Size: 5651 ApaLI G'TGCA_C Cuts at: 130 1885 4421 130 Size: 1755 2536 1360 Fragments arranged by size: 2536 1755 1360 ApoI r'AATT_y Cuts at: 1026 1055 1138 2497 2521 2655 5004 5260 1026 Size: 29 83 1359 24 134 2349 256 1417 Fragments arranged by size: 2349 1417 1359 256 134 83 29 24 AseI AT'TA_AT Cuts at: 20 20 Size: 5651 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5651 AvaI C'yCGr_G Cuts at: 206 635 656 689 2671 206 Size: 429 21 33 1982 3186 Fragments arranged by size: 3186 1982 429 33 21 BamHI G'GATC_C Cuts at: 2676 2676 Size: 5651 BanI G'GyrC_C Cuts at: 261 477 2667 3081 3329 3364 261 Size: 216 2190 414 248 35 2548 Fragments arranged by size: 2548 2190 414 248 216 35 BanII G_rGCy'C Cuts at: 127 636 1784 1818 2665 3695 127 Size: 509 1148 34 847 1030 2083 Fragments arranged by size: 2083 1148 1030 847 509 34 BbeI G_GCGC'C Cuts at: 3085 3333 3085 Size: 248 5403 BbsI GAAGACnn'nnnn_ Cuts at: 848 1808 848 Size: 960 4691 BbvCI CC'TCA_GC Cuts at: 450 450 Size: 5651 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 111 145 314 348 111 Size: 34 169 34 5414 Fragments arranged by size: 5414 169 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1026 3549 4310 5651 1026 Size: 2523 761 1341 1026 Fragments arranged by size: 2523 1341 1026 761 BclI T'GATC_A Cuts at: 1905 3171 1905 Size: 1266 4385 BglI GCCn_nnn'nGGC Cuts at: 640 649 640 Size: 9 5642 BglII A'GATC_T Cuts at: 3166 5203 3166 Size: 2037 3614 BlpI GC'TnA_GC Cuts at: 518 2763 518 Size: 2245 3406 Bme1580I G_kGCm'C Cuts at: 134 636 1889 2563 3276 3369 4425 134 Size: 502 1253 674 713 93 1056 1360 Fragments arranged by size: 1360 1253 1056 713 674 502 93 BmrI ACTGGGnnnn_n' Cuts at: 2001 2863 2944 3279 5423 2001 Size: 862 81 335 2144 2229 Fragments arranged by size: 2229 2144 862 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4018 4018 Size: 5651 Bpu10I CC'TnA_GC Cuts at: 450 948 1448 450 Size: 498 500 4653 Fragments arranged by size: 4653 500 498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2827 3706 4198 4496 2827 Size: 879 492 298 3982 Fragments arranged by size: 3982 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 628 5355 628 Size: 4727 924 BsaAI yAC'GTr Cuts at: 1305 3004 1305 Size: 1699 3952 BsaBI GATnn'nnATC Cuts at: 6 3165 5202 6 Size: 3159 2037 455 Fragments arranged by size: 3159 2037 455 BsaHI Gr'CG_yC Cuts at: 3082 3330 4032 5528 3082 Size: 248 702 1496 3205 Fragments arranged by size: 3205 1496 702 248 BsaWI w'CCGG_w Cuts at: 242 2840 3361 4039 4313 4460 4871 242 Size: 2598 521 678 274 147 411 1022 Fragments arranged by size: 2598 1022 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 606 636 1198 1228 606 Size: 30 562 30 5029 Fragments arranged by size: 5029 562 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 192 265 438 523 624 627 676 2064 2209 Size: 73 173 85 101 3 49 1388 145 Cuts at: 2209 192 Size: 3634 Fragments arranged by size: 3634 1388 173 145 101 85 73 49 3 BseYI C'CCAG_C Cuts at: 3076 3834 4411 4907 3076 Size: 758 577 496 3820 Fragments arranged by size: 3820 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 496 496 Size: 5651 BsiEI CG_ry'CG Cuts at: 104 440 3193 3239 4447 104 Size: 336 2753 46 1208 1308 Fragments arranged by size: 2753 1308 1208 336 46 BsiHKAI G_wGCw'C Cuts at: 134 1166 1818 1889 2665 3443 3633 4425 134 Size: 1032 652 71 776 778 190 792 1360 Fragments arranged by size: 1360 1032 792 778 776 652 190 71 BsmI GAATG_Cn' Cuts at: 1979 2060 1979 Size: 81 5570 BsmBI CGTCTCn'nnnn_ Cuts at: 171 171 Size: 5651 Bsp1286I G_dGCh'C Cuts at: 127 134 636 1166 1784 1818 1889 2563 2665 Size: 7 502 530 618 34 71 674 102 Cuts at: 2665 3276 3369 3443 3633 3695 4425 127 Size: 611 93 74 190 62 730 1353 Fragments arranged by size: 1353 730 674 618 611 530 502 190 102 93 74 71 62 34 7 BspEI T'CCGG_A Cuts at: 242 4871 242 Size: 4629 1022 BspHI T'CATG_A Cuts at: 2223 4827 2223 Size: 2604 3047 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2701 2915 3217 3598 2701 Size: 214 302 381 4754 Fragments arranged by size: 4754 381 302 214 BsrBI CCG'CTC Cuts at: 50 3943 3997 5481 5646 50 Size: 3893 54 1484 165 55 Fragments arranged by size: 3893 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3563 3563 Size: 5651 BsrFI r'CCGG_y Cuts at: 321 474 643 3649 3830 4039 321 Size: 153 169 3006 181 209 1933 Fragments arranged by size: 3006 1933 209 181 169 153 BsrGI T'GTAC_A Cuts at: 1591 1591 Size: 5651 BssSI C'ACGA_G Cuts at: 1100 3922 4280 1100 Size: 2822 358 2471 Fragments arranged by size: 2822 2471 358 BstAPI GCAn_nnn'nTGC Cuts at: 3028 3028 Size: 5651 BstBI TT'CG_AA Cuts at: 4012 4012 Size: 5651 BstXI CCAn_nnnn'nTGG Cuts at: 1174 1174 Size: 5651 BstYI r'GATC_y Cuts at: 1 2676 3143 3166 3501 3747 4759 4845 4857 Size: 2675 467 23 335 246 1012 86 12 Cuts at: 4857 5203 1 Size: 346 449 Fragments arranged by size: 2675 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 587 587 Size: 5651 BtgI C'CryG_G Cuts at: 107 146 265 1600 107 Size: 39 119 1335 4158 Fragments arranged by size: 4158 1335 119 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3601 3782 3945 3601 Size: 181 163 5307 Fragments arranged by size: 5307 181 163 BtsI GCAGTG_nn' Cuts at: 1594 2894 1594 Size: 1300 4351 DraI TTT'AAA Cuts at: 1143 1586 2546 2650 1143 Size: 443 960 104 4144 Fragments arranged by size: 4144 960 443 104 DrdI GACnn_nn'nnGTC Cuts at: 3357 4215 3357 Size: 858 4793 EaeI y'GGCC_r Cuts at: 437 3236 3410 3801 3828 5173 437 Size: 2799 174 391 27 1345 915 Fragments arranged by size: 2799 1345 915 391 174 27 EagI C'GGCC_G Cuts at: 437 3236 437 Size: 2799 2852 EarI CTCTTCn'nnn_ Cuts at: 408 1247 1565 3674 3884 408 Size: 839 318 2109 210 2175 Fragments arranged by size: 2175 2109 839 318 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 219 3859 4169 4315 5461 127 Size: 92 3640 310 146 1146 317 Fragments arranged by size: 3640 1146 317 310 146 92 EcoICRI GAG'CTC Cuts at: 1816 2663 1816 Size: 847 4804 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1741 1897 1967 3476 3908 4655 4923 1741 Size: 156 70 1509 432 747 268 2469 Fragments arranged by size: 2469 1509 747 432 268 156 70 EcoNI CCTnn'n_nnAGG Cuts at: 1175 1175 Size: 5651 EcoO109I rG'GnC_Cy Cuts at: 210 632 650 2790 210 Size: 422 18 2140 3071 Fragments arranged by size: 3071 2140 422 18 EcoRI G'AATT_C Cuts at: 2655 2655 Size: 5651 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3670 3670 Size: 5651 FspI TGC'GCA Cuts at: 3432 3432 Size: 5651 HaeII r_GCGC'y Cuts at: 3085 3333 4355 5334 3085 Size: 248 1022 979 3402 Fragments arranged by size: 3402 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 606 638 683 715 1800 1832 2236 2268 3493 Size: 32 45 32 1085 32 404 32 1225 Cuts at: 3493 3523 3525 3555 606 Size: 30 2 30 2702 Fragments arranged by size: 2702 1225 1085 404 45 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2690 2690 Size: 5651 HindIII A'AGCT_T Cuts at: 1506 1506 Size: 5651 KasI G'GCGC_C Cuts at: 3081 3329 3081 Size: 248 5403 KpnI G_GTAC'C Cuts at: 2671 2671 Size: 5651 MluI A'CGCG_T Cuts at: 5084 5084 Size: 5651 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1106 1547 1848 4322 4506 1106 Size: 441 301 2474 184 2251 Fragments arranged by size: 2474 2251 441 301 184 MscI TGG'CCA Cuts at: 3412 3412 Size: 5651 MslI CAynn'nnrTG Cuts at: 1599 3640 3767 1599 Size: 2041 127 3483 Fragments arranged by size: 3483 2041 127 MspA1I CmG'CkG Cuts at: 148 267 293 312 430 556 684 1861 2146 Size: 119 26 19 118 126 128 1177 285 Cuts at: 2146 2762 3076 3436 4449 4694 148 Size: 616 314 360 1013 245 1105 Fragments arranged by size: 1177 1105 1013 616 360 314 285 245 128 126 119 118 26 19 NaeI GCC'GGC Cuts at: 323 645 3832 323 Size: 322 3187 2142 Fragments arranged by size: 3187 2142 322 NarI GG'CG_CC Cuts at: 3082 3330 3082 Size: 248 5403 NcoI C'CATG_G Cuts at: 107 1600 107 Size: 1493 4158 NgoMIV G'CCGG_C Cuts at: 321 643 3830 321 Size: 322 3187 2142 Fragments arranged by size: 3187 2142 322 NspI r_CATG'y Cuts at: 1566 2704 3204 4111 1566 Size: 1138 500 907 3106 Fragments arranged by size: 3106 1138 907 500 PciI A'CATG_T Cuts at: 1562 4107 1562 Size: 2545 3106 PflMI CCAn_nnn'nTGG Cuts at: 1664 1664 Size: 5651 PfoI T'CCnGG_A Cuts at: 1674 1674 Size: 5651 PmeI GTTT'AAAC Cuts at: 2650 2650 Size: 5651 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1452 1484 4790 4822 1452 Size: 32 3306 32 2281 Fragments arranged by size: 3306 2281 32 32 PpuMI rG'GwC_Cy Cuts at: 210 210 Size: 5651 PspOMI G'GGCC_C Cuts at: 632 632 Size: 5651 PstI C_TGCA'G Cuts at: 317 1296 2698 3383 317 Size: 979 1402 685 2585 Fragments arranged by size: 2585 1402 979 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5651 PvuII CAG'CTG Cuts at: 312 1861 2146 3076 3436 312 Size: 1549 285 930 360 2527 Fragments arranged by size: 2527 1549 930 360 285 RsrII CG'GwC_CG Cuts at: 3846 3846 Size: 5651 SacI G_AGCT'C Cuts at: 1818 2665 1818 Size: 847 4804 SacII CC_GC'GG Cuts at: 149 268 149 Size: 119 5532 SalI G'TCGA_C Cuts at: 2688 2688 Size: 5651 SanDI GG'GwC_CC Cuts at: 210 210 Size: 5651 SapI GCTCTTCn'nnn_ Cuts at: 3674 3884 3674 Size: 210 5441 SbfI CC_TGCA'GG Cuts at: 2698 2698 Size: 5651 ScaI AGT'ACT Cuts at: 5168 5168 Size: 5651 SfcI C'TryA_G Cuts at: 33 313 1200 1292 1856 2432 2694 3379 4372 Size: 280 887 92 564 576 262 685 993 Cuts at: 4372 4563 33 Size: 191 1121 Fragments arranged by size: 1121 993 887 685 576 564 280 262 191 92 SfiI GGCCn_nnn'nGGCC Cuts at: 640 649 640 Size: 9 5642 SfoI GGC'GCC Cuts at: 3083 3331 3083 Size: 248 5403 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5651 SmaI CCC'GGG Cuts at: 208 658 2673 208 Size: 450 2015 3186 Fragments arranged by size: 3186 2015 450 SmlI C'TyrA_G Cuts at: 2806 3721 4213 4475 2806 Size: 915 492 262 3982 Fragments arranged by size: 3982 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5651 SphI G_CATG'C Cuts at: 2704 3204 2704 Size: 500 5151 SspI AAT'ATT Cuts at: 2418 2418 Size: 5651 StyI C'CwwG_G Cuts at: 107 1600 2110 2309 2785 3139 107 Size: 1493 510 199 476 354 2619 Fragments arranged by size: 2619 1493 510 476 354 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3234 3367 3826 4035 3234 Size: 133 459 209 4850 Fragments arranged by size: 4850 459 209 133 TatI w'GTAC_w Cuts at: 1591 5166 1591 Size: 3575 2076 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 426 458 2542 2574 5168 5200 426 Size: 32 2084 32 2594 32 877 Fragments arranged by size: 2594 2084 877 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1431 3448 1431 Size: 2017 3634 XbaI T'CTAG_A Cuts at: 2682 2682 Size: 5651 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3146 3146 Size: 5651 XmaI C'CCGG_G Cuts at: 206 656 2671 206 Size: 450 2015 3186 Fragments arranged by size: 3186 2015 450 XmnI GAAnn'nnTTC Cuts at: 1464 1964 2060 1464 Size: 500 96 5055 Fragments arranged by size: 5055 500 96 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AscI AvrII BaeI BfrBI BmgBI BmtI BpmI BsiWI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SexAI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI