(Linear) (Six-base) MAPSORT of: KIEE2454.seq Check: 1345 from: 1 to: 2538 KIEE2454.orf With 182 enzymes: SgfI * February 8, 2013 18:16 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1633 1789 1859 2538 Size: 1633 156 70 679 Fragments arranged by size: 1633 679 156 70 AflIII A'CryG_T Cuts at: 0 1454 2538 Size: 1454 1084 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 220 2538 Size: 220 2318 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 925 957 1565 1597 2538 Size: 925 32 608 32 941 Fragments arranged by size: 941 925 608 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1756 2538 Size: 1756 782 ApaI G_GGCC'C Cuts at: 0 528 2538 Size: 528 2010 ApaLI G'TGCA_C Cuts at: 0 22 1777 2538 Size: 22 1755 761 Fragments arranged by size: 1755 761 22 ApoI r'AATT_y Cuts at: 0 918 947 1030 2389 2413 2538 Size: 918 29 83 1359 24 125 Fragments arranged by size: 1359 918 125 83 29 24 AvaI C'yCGr_G Cuts at: 0 98 527 548 581 2538 Size: 98 429 21 33 1957 Fragments arranged by size: 1957 429 98 33 21 BanI G'GyrC_C Cuts at: 0 153 369 2538 Size: 153 216 2169 Fragments arranged by size: 2169 216 153 BanII G_rGCy'C Cuts at: 0 19 528 1676 1710 2538 Size: 19 509 1148 34 828 Fragments arranged by size: 1148 828 509 34 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 740 1700 2538 Size: 740 960 838 Fragments arranged by size: 960 838 740 BbvCI CC'TCA_GC Cuts at: 0 342 2538 Size: 342 2196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3 37 206 240 2538 Size: 3 34 169 34 2298 Fragments arranged by size: 2298 169 34 34 3 BciVI GTATCCnnnnn_n' Cuts at: 0 918 2538 Size: 918 1620 BclI T'GATC_A Cuts at: 0 1797 2538 Size: 1797 741 BglI GCCn_nnn'nGGC Cuts at: 0 532 541 2538 Size: 532 9 1997 Fragments arranged by size: 1997 532 9 BlpI GC'TnA_GC Cuts at: 0 410 2538 Size: 410 2128 Bme1580I G_kGCm'C Cuts at: 0 26 528 1781 2455 2538 Size: 26 502 1253 674 83 Fragments arranged by size: 1253 674 502 83 26 BmrI ACTGGGnnnn_n' Cuts at: 0 1893 2538 Size: 1893 645 Bpu10I CC'TnA_GC Cuts at: 0 342 840 1340 2538 Size: 342 498 500 1198 Fragments arranged by size: 1198 500 498 342 BsaI GGTCTCn'nnnn_ Cuts at: 0 520 2538 Size: 520 2018 BsaAI yAC'GTr Cuts at: 0 1197 2538 Size: 1197 1341 BsaWI w'CCGG_w Cuts at: 0 134 2538 Size: 134 2404 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 498 528 1090 1120 2538 Size: 498 30 562 30 1418 Fragments arranged by size: 1418 562 498 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 84 157 330 415 516 519 568 1956 Size: 84 73 173 85 101 3 49 1388 Cuts at: 1956 2101 2538 Size: 145 437 Fragments arranged by size: 1388 437 173 145 101 85 84 73 49 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 2538 Size: 388 2150 BsiEI CG_ry'CG Cuts at: 0 332 2538 Size: 332 2206 BsiHKAI G_wGCw'C Cuts at: 0 26 1058 1710 1781 2538 Size: 26 1032 652 71 757 Fragments arranged by size: 1032 757 652 71 26 BsmI GAATG_Cn' Cuts at: 0 1871 1952 2538 Size: 1871 81 586 Fragments arranged by size: 1871 586 81 BsmBI CGTCTCn'nnnn_ Cuts at: 0 63 2538 Size: 63 2475 Bsp1286I G_dGCh'C Cuts at: 0 19 26 528 1058 1676 1710 1781 2455 Size: 19 7 502 530 618 34 71 674 Cuts at: 2455 2538 Size: 83 Fragments arranged by size: 674 618 530 502 83 71 34 19 7 BspEI T'CCGG_A Cuts at: 0 134 2538 Size: 134 2404 BspHI T'CATG_A Cuts at: 0 2115 2538 Size: 2115 423 BsrFI r'CCGG_y Cuts at: 0 213 366 535 2538 Size: 213 153 169 2003 Fragments arranged by size: 2003 213 169 153 BsrGI T'GTAC_A Cuts at: 0 1483 2538 Size: 1483 1055 BssSI C'ACGA_G Cuts at: 0 992 2538 Size: 992 1546 BstXI CCAn_nnnn'nTGG Cuts at: 0 1066 2538 Size: 1066 1472 Bsu36I CC'TnA_GG Cuts at: 0 479 2538 Size: 479 2059 BtgI C'CryG_G Cuts at: 0 38 157 1492 2538 Size: 38 119 1335 1046 Fragments arranged by size: 1335 1046 119 38 BtsI GCAGTG_nn' Cuts at: 0 1486 2538 Size: 1486 1052 DraI TTT'AAA Cuts at: 0 1035 1478 2438 2538 Size: 1035 443 960 100 Fragments arranged by size: 1035 960 443 100 EaeI y'GGCC_r Cuts at: 0 329 2538 Size: 329 2209 EagI C'GGCC_G Cuts at: 0 329 2538 Size: 329 2209 EarI CTCTTCn'nnn_ Cuts at: 0 300 1139 1457 2538 Size: 300 839 318 1081 Fragments arranged by size: 1081 839 318 300 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 111 2538 Size: 19 92 2427 Fragments arranged by size: 2427 92 19 EcoICRI GAG'CTC Cuts at: 0 1708 2538 Size: 1708 830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1633 1789 1859 2538 Size: 1633 156 70 679 Fragments arranged by size: 1633 679 156 70 EcoNI CCTnn'n_nnAGG Cuts at: 0 1067 2538 Size: 1067 1471 EcoO109I rG'GnC_Cy Cuts at: 0 102 524 542 2538 Size: 102 422 18 1996 Fragments arranged by size: 1996 422 102 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 498 530 575 607 1692 1724 2128 2160 Size: 498 32 45 32 1085 32 404 32 Cuts at: 2160 2538 Size: 378 Fragments arranged by size: 1085 498 404 378 45 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1398 2538 Size: 1398 1140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 998 1439 1740 2538 Size: 998 441 301 798 Fragments arranged by size: 998 798 441 301 MslI CAynn'nnrTG Cuts at: 0 1491 2538 Size: 1491 1047 MspA1I CmG'CkG Cuts at: 0 40 159 185 204 322 448 576 1753 Size: 40 119 26 19 118 126 128 1177 Cuts at: 1753 2038 2538 Size: 285 500 Fragments arranged by size: 1177 500 285 128 126 119 118 40 26 19 NaeI GCC'GGC Cuts at: 0 215 537 2538 Size: 215 322 2001 Fragments arranged by size: 2001 322 215 NcoI C'CATG_G Cuts at: 0 1492 2538 Size: 1492 1046 NgoMIV G'CCGG_C Cuts at: 0 213 535 2538 Size: 213 322 2003 Fragments arranged by size: 2003 322 213 NspI r_CATG'y Cuts at: 0 1458 2538 Size: 1458 1080 PciI A'CATG_T Cuts at: 0 1454 2538 Size: 1454 1084 PflMI CCAn_nnn'nTGG Cuts at: 0 1556 2538 Size: 1556 982 PfoI T'CCnGG_A Cuts at: 0 1566 2538 Size: 1566 972 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1344 1376 2538 Size: 1344 32 1162 Fragments arranged by size: 1344 1162 32 PpuMI rG'GwC_Cy Cuts at: 0 102 2538 Size: 102 2436 PspOMI G'GGCC_C Cuts at: 0 524 2538 Size: 524 2014 PstI C_TGCA'G Cuts at: 0 209 1188 2538 Size: 209 979 1350 Fragments arranged by size: 1350 979 209 PvuII CAG'CTG Cuts at: 0 204 1753 2038 2538 Size: 204 1549 285 500 Fragments arranged by size: 1549 500 285 204 SacI G_AGCT'C Cuts at: 0 1710 2538 Size: 1710 828 SacII CC_GC'GG Cuts at: 0 41 160 2538 Size: 41 119 2378 Fragments arranged by size: 2378 119 41 SanDI GG'GwC_CC Cuts at: 0 102 2538 Size: 102 2436 SfcI C'TryA_G Cuts at: 0 205 1092 1184 1748 2324 2538 Size: 205 887 92 564 576 214 Fragments arranged by size: 887 576 564 214 205 92 SfiI GGCCn_nnn'nGGCC Cuts at: 0 532 541 2538 Size: 532 9 1997 Fragments arranged by size: 1997 532 9 SmaI CCC'GGG Cuts at: 0 100 550 2538 Size: 100 450 1988 Fragments arranged by size: 1988 450 100 SspI AAT'ATT Cuts at: 0 2310 2538 Size: 2310 228 StyI C'CwwG_G Cuts at: 0 1492 2002 2201 2538 Size: 1492 510 199 337 Fragments arranged by size: 1492 510 337 199 TatI w'GTAC_w Cuts at: 0 1483 2538 Size: 1483 1055 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 318 350 2434 2466 2538 Size: 318 32 2084 32 72 Fragments arranged by size: 2084 318 72 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1323 2538 Size: 1323 1215 XmaI C'CCGG_G Cuts at: 0 98 548 2538 Size: 98 450 1990 Fragments arranged by size: 1990 450 98 XmnI GAAnn'nnTTC Cuts at: 0 1356 1856 1952 2538 Size: 1356 500 96 586 Fragments arranged by size: 1356 586 500 96 Enzymes that do cut: AcuI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmrI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrFI BsrGI BssSI BstXI Bsu36I BtgI BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI SmaI SspI StyI TatI TstI Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI BbeI BfrBI BglII BmgBI BmtI BplI BpmI BpuEI BsaBI BsaHI BseYI BsiWI BspMI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII XbaI XcmI XhoI ZraI