################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbeI BbvCI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AlwNI 772 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 999 CCTTAGG CC^T[ACGT]AGG ApaI 1178 GGGCCC GGGCC^C AspI 1020 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 52 CCCGAG C^[CT]CG[GA]G AviII 599 TGCGCA TGC^GCA BalI 849 TGGCCA TGG^CCA BamHI 537 GGATCC G^GATCC BbrPI 1650 CACGTG CAC^GTG BbsI 1899 GAAGAC GAAGAC(2/6) BcgI 1398 GCAGCTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1824 GGATAC GTATCC(6/5) BglI 1681 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1899 GAAGAC GAAGAC(2/6) BsaAI 1650 CACGTG [CT]AC^GT[GA] BsaWI 1088 ACCGGA [AT]^CCGG[AT] BsgI 12 CTGCAC GTGCAG(16/14) BsoBI 52 CCCGAG C^[CT]CG[GA]G BspHI 695 TCATGA T^CATGA BstEII 1884 GGTAACC G^GT[ACGT]ACC BstPI 1884 GGTAACC G^GT[ACGT]ACC Bsu36I 999 CCTTAGG CC^T[ACGT]AGG DraI 582 TTTAAA TTT^AAA DrdI 1087 GACCGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 999 CCTTAGG CC^T[ACGT]AGG EcoNI 971 CCTCATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1884 GGTAACC G^GT[ACGT]ACC EcoRI 613 GAATTC G^AATTC EcoT14I 1568 CCTTGG C^C[AT][AT]GG FauI 93 GCGGG CCCGC(4/6) FspI 599 TGCGCA TGC^GCA HaeII 48 AGCGCC [GA]GCGC^[CT] HincII 715 GTCAAC GT[CT]^[GA]AC HindII 715 GTCAAC GT[CT]^[GA]AC HindIII 586 AAGCTT A^AGCTT HphI 1158 TCACC GGTGA(8/7) MluNI 849 TGGCCA TGG^CCA MscI 849 TGGCCA TGG^CCA NheI 679 GCTAGC G^CTAGC NspI 1364 ACATGC [GA]CATG^[CT] PflFI 1020 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 660 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1650 CACGTG CAC^GTG PmlI 1650 CACGTG CAC^GTG PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT] PstI 1633 CTGCAG CTGCA^G RcaI 695 TCATGA T^CATGA SacI 1672 GAGCTC GAGCT^C ScaI 965 AGTACT AGT^ACT SmlI 1411 CTTGAG C^T[CT][GA]AG StyI 1568 CCTTGG C^C[AT][AT]GG Tth111I 1020 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 660 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1757 CCAGAGACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 746 GTAC GT^AC 966 GTAC AflIII 1649 ACACGT A^C[GA][CT]GT 1651 ACGTGT AspHI 173 GTGCTC G[AT]GC[AT]^C 1672 GAGCTC BanI 34 GGTGCC G^G[CT][GA]CC 2114 GGCACC BanII 1178 GGGCCC G[GA]GC[CT]^C 1672 GAGCTC BcnI 84 CCCGG CC^[GC]GG 1071 CCGGG BglII 824 AGATCT A^GATCT 1743 AGATCT BsaJI 53 CCGAGG C^C[ACGT][ACGT]GG 1568 CCTTGG BsiHKAI 173 GTGCTC G[AT]GC[AT]^C 1672 GAGCTC BsrDI 1443 CATTGC GCAATG(2/0) 1418 GCAATG DraII 773 AGGACCT [GA]G^G[ACGT]CC[CT] 1177 AGGGCCC EaeI 849 TGGCCA [CT]^GGCC[GA] 1688 TGGCCG EcoO109I 773 AGGACCT [GA]G^G[ACGT]CC[CT] 1177 AGGGCCC NciI 84 CCCGG CC^[GC]GG 1071 CCGGG RsaI 746 GTAC GT^AC 966 GTAC SapI 902 GCTCTTC GCTCTTC(1/4) 2005 GCTCTTC SfcI 788 CTGTAG C^T[GA][CT]AG 1633 CTGCAG TfiI 331 GAATC G^A[AT]TC 1053 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AvaII BbvI BfaI BmyI BpmI BsaI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII