(Circular) (Six-base) MAPSORT of: pF1KE2446.seq Check: 3187 from: 1 to: 5258 pF1KE2446 5258 bp With 182 enzymes: SgfI * February 8, 2013 18:16 .. AccI GT'mk_AC Cuts at: 2296 2296 Size: 5258 Acc65I G'GTAC_C Cuts at: 2274 2274 Size: 5258 AclI AA'CG_TT Cuts at: 5098 5098 Size: 5258 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 800 878 1021 1303 1834 2049 3083 3515 4262 Size: 78 143 282 531 215 1034 432 747 Cuts at: 4262 4530 800 Size: 268 1528 Fragments arranged by size: 1528 1034 747 531 432 282 268 215 143 78 AflIII A'CryG_T Cuts at: 1757 1759 2610 3714 4691 1757 Size: 2 851 1104 977 2324 Fragments arranged by size: 2324 1104 977 851 2 AgeI A'CCGG_T Cuts at: 3646 3646 Size: 5258 AlwNI CAG_nnn'CTG Cuts at: 885 2563 4130 885 Size: 1678 1567 2013 Fragments arranged by size: 2013 1678 1567 ApaI G_GGCC'C Cuts at: 1290 1290 Size: 5258 ApaLI G'TGCA_C Cuts at: 4028 4028 Size: 5258 ApoI r'AATT_y Cuts at: 271 616 721 1383 1408 2262 4611 4867 271 Size: 345 105 662 25 854 2349 256 662 Fragments arranged by size: 2349 854 662 662 345 256 105 25 AseI AT'TA_AT Cuts at: 20 20 Size: 5258 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5258 AvaI C'yCGr_G Cuts at: 160 2278 160 Size: 2118 3140 BamHI G'GATC_C Cuts at: 645 2283 645 Size: 1638 3620 BanI G'GyrC_C Cuts at: 142 2222 2274 2688 2936 2971 142 Size: 2080 52 414 248 35 2429 Fragments arranged by size: 2429 2080 414 248 52 35 BanII G_rGCy'C Cuts at: 1290 1784 2272 3302 1290 Size: 494 488 1030 3246 Fragments arranged by size: 3246 1030 494 488 BbeI G_GCGC'C Cuts at: 2692 2940 2692 Size: 248 5010 BbsI GAAGACnn'nnnn_ Cuts at: 2014 2014 Size: 5258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1495 1529 1495 Size: 34 5224 BciVI GTATCCnnnnn_n' Cuts at: 1926 3156 3917 5258 1926 Size: 1230 761 1341 1926 Fragments arranged by size: 1926 1341 1230 761 BclI T'GATC_A Cuts at: 2778 2778 Size: 5258 BglI GCCn_nnn'nGGC Cuts at: 1795 1795 Size: 5258 BglII A'GATC_T Cuts at: 932 1851 2773 4810 932 Size: 919 922 2037 1380 Fragments arranged by size: 2037 1380 922 919 BlpI GC'TnA_GC Cuts at: 2370 2370 Size: 5258 Bme1580I G_kGCm'C Cuts at: 1290 2883 2976 4032 1290 Size: 1593 93 1056 2516 Fragments arranged by size: 2516 1593 1056 93 BmgBI CAC'GTC Cuts at: 1485 1485 Size: 5258 BmrI ACTGGGnnnn_n' Cuts at: 1139 2219 2470 2551 2886 5030 1139 Size: 1080 251 81 335 2144 1367 Fragments arranged by size: 2144 1367 1080 335 251 81 BmtI G_CTAG'C Cuts at: 791 791 Size: 5258 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3625 3625 Size: 5258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1264 1360 1379 1618 1669 1897 1264 Size: 96 19 239 51 228 4625 Fragments arranged by size: 4625 239 228 96 51 19 Bpu10I CC'TnA_GC Cuts at: 1952 1952 Size: 5258 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1540 2434 3313 3805 4103 1540 Size: 894 879 492 298 2695 Fragments arranged by size: 2695 894 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 695 1209 1862 4962 695 Size: 514 653 3100 991 Fragments arranged by size: 3100 991 653 514 BsaAI yAC'GTr Cuts at: 1760 2611 1760 Size: 851 4407 BsaBI GATnn'nnATC Cuts at: 6 2772 4809 6 Size: 2766 2037 455 Fragments arranged by size: 2766 2037 455 BsaHI Gr'CG_yC Cuts at: 2689 2937 3639 5135 2689 Size: 248 702 1496 2812 Fragments arranged by size: 2812 1496 702 248 BsaWI w'CCGG_w Cuts at: 1196 2447 2968 3646 3920 4067 4478 1196 Size: 1251 521 678 274 147 411 1976 Fragments arranged by size: 1976 1251 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1246 1276 2062 2092 1246 Size: 30 786 30 4412 Fragments arranged by size: 4412 786 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 586 1068 1615 1666 1669 586 Size: 482 547 51 3 4175 Fragments arranged by size: 4175 547 482 51 3 BseYI C'CCAG_C Cuts at: 138 1208 1643 2683 3441 4018 4514 138 Size: 1070 435 1040 758 577 496 882 Fragments arranged by size: 1070 1040 882 758 577 496 435 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 105 105 Size: 5258 BsiEI CG_ry'CG Cuts at: 104 2800 2846 4054 104 Size: 2696 46 1208 1308 Fragments arranged by size: 2696 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 285 1784 2272 3050 3240 4032 285 Size: 1499 488 778 190 792 1511 Fragments arranged by size: 1511 1499 792 778 488 190 Bsp1286I G_dGCh'C Cuts at: 285 1290 1784 2272 2883 2976 3050 3240 3302 Size: 1005 494 488 611 93 74 190 62 Cuts at: 3302 4032 285 Size: 730 1511 Fragments arranged by size: 1511 1005 730 611 494 488 190 93 74 62 BspEI T'CCGG_A Cuts at: 4478 4478 Size: 5258 BspHI T'CATG_A Cuts at: 803 4434 803 Size: 3631 1627 BspMI ACCTGCnnnn'nnnn_ Cuts at: 713 893 954 2308 2522 2824 3205 713 Size: 180 61 1354 214 302 381 2766 Fragments arranged by size: 2766 1354 381 302 214 180 61 BsrBI CCG'CTC Cuts at: 50 3550 3604 5088 5253 50 Size: 3500 54 1484 165 55 Fragments arranged by size: 3500 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1533 1550 3170 1533 Size: 17 1620 3621 Fragments arranged by size: 3621 1620 17 BsrFI r'CCGG_y Cuts at: 3256 3437 3646 3256 Size: 181 209 4868 Fragments arranged by size: 4868 209 181 BssSI C'ACGA_G Cuts at: 3529 3887 3529 Size: 358 4900 BstAPI GCAn_nnn'nTGC Cuts at: 2635 2635 Size: 5258 BstBI TT'CG_AA Cuts at: 3619 3619 Size: 5258 BstEII G'GTnAC_C Cuts at: 1992 1992 Size: 5258 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 5258 BstYI r'GATC_y Cuts at: 1 645 932 1851 2283 2750 2773 3108 3354 Size: 644 287 919 432 467 23 335 246 Cuts at: 3354 4366 4452 4464 4810 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 919 644 467 449 432 346 335 287 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1108 1108 Size: 5258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3208 3389 3552 3208 Size: 181 163 4914 Fragments arranged by size: 4914 181 163 BtsI GCAGTG_nn' Cuts at: 2501 2501 Size: 5258 DraI TTT'AAA Cuts at: 692 2257 692 Size: 1565 3693 DrdI GACnn_nn'nnGTC Cuts at: 1201 2964 3822 1201 Size: 1763 858 2637 Fragments arranged by size: 2637 1763 858 EaeI y'GGCC_r Cuts at: 957 1796 2843 3017 3408 3435 4780 957 Size: 839 1047 174 391 27 1345 1435 Fragments arranged by size: 1435 1345 1047 839 391 174 27 EagI C'GGCC_G Cuts at: 2843 2843 Size: 5258 EarI CTCTTCn'nnn_ Cuts at: 746 854 1017 1021 1322 2120 3281 3491 746 Size: 108 163 4 301 798 1161 210 2513 Fragments arranged by size: 2513 1161 798 301 210 163 108 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3466 3776 3922 5068 3466 Size: 310 146 1146 3656 Fragments arranged by size: 3656 1146 310 146 EcoICRI GAG'CTC Cuts at: 1782 2270 1782 Size: 488 4770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 800 878 1021 1264 1303 1360 1379 1618 1669 Size: 78 143 243 39 57 19 239 51 Cuts at: 1669 1834 1897 2049 3083 3515 4262 4530 800 Size: 165 63 152 1034 432 747 268 1528 Fragments arranged by size: 1528 1034 747 432 268 243 239 165 152 143 78 63 57 51 39 19 EcoNI CCTnn'n_nnAGG Cuts at: 1083 1083 Size: 5258 EcoO109I rG'GnC_Cy Cuts at: 882 1286 2397 882 Size: 404 1111 3743 Fragments arranged by size: 3743 1111 404 EcoRI G'AATT_C Cuts at: 721 2262 721 Size: 1541 3717 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 931 3277 931 Size: 2346 2912 FspI TGC'GCA Cuts at: 709 3039 709 Size: 2330 2928 HaeII r_GCGC'y Cuts at: 160 2692 2940 3962 4941 160 Size: 2532 248 1022 979 477 Fragments arranged by size: 2532 1022 979 477 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 292 324 655 687 785 817 2062 2094 3100 Size: 32 331 32 98 32 1245 32 1006 Cuts at: 3100 3130 3132 3162 292 Size: 30 2 30 2388 Fragments arranged by size: 2388 1245 1006 331 98 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 825 2297 825 Size: 1472 3786 HindIII A'AGCT_T Cuts at: 694 694 Size: 5258 KasI G'GCGC_C Cuts at: 2688 2936 2688 Size: 248 5010 KpnI G_GTAC'C Cuts at: 2278 2278 Size: 5258 MluI A'CGCG_T Cuts at: 4691 4691 Size: 5258 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1001 1289 1421 3929 4113 1001 Size: 288 132 2508 184 2146 Fragments arranged by size: 2508 2146 288 184 132 MscI TGG'CCA Cuts at: 959 3019 959 Size: 2060 3198 MslI CAynn'nnrTG Cuts at: 3247 3374 3247 Size: 127 5131 MspA1I CmG'CkG Cuts at: 1329 1509 1647 2055 2369 2683 3043 4056 4301 Size: 180 138 408 314 314 360 1013 245 Cuts at: 4301 1329 Size: 2286 Fragments arranged by size: 2286 1013 408 360 314 314 245 180 138 NaeI GCC'GGC Cuts at: 3439 3439 Size: 5258 NarI GG'CG_CC Cuts at: 2689 2937 2689 Size: 248 5010 NgoMIV G'CCGG_C Cuts at: 3437 3437 Size: 5258 NheI G'CTAG_C Cuts at: 787 787 Size: 5258 NspI r_CATG'y Cuts at: 1476 2311 2811 3718 1476 Size: 835 500 907 3016 Fragments arranged by size: 3016 907 835 500 PciI A'CATG_T Cuts at: 3714 3714 Size: 5258 PflMI CCAn_nnn'nTGG Cuts at: 774 774 Size: 5258 PmeI GTTT'AAAC Cuts at: 2257 2257 Size: 5258 PmlI CAC'GTG Cuts at: 1760 1760 Size: 5258 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 404 436 4397 4429 404 Size: 32 3961 32 1233 Fragments arranged by size: 3961 1233 32 32 PpuMI rG'GwC_Cy Cuts at: 882 882 Size: 5258 PspOMI G'GGCC_C Cuts at: 1286 1286 Size: 5258 PstI C_TGCA'G Cuts at: 1745 2305 2990 1745 Size: 560 685 4013 Fragments arranged by size: 4013 685 560 PvuI CG_AT'CG Cuts at: 104 104 Size: 5258 PvuII CAG'CTG Cuts at: 1329 1509 1647 2055 2683 3043 1329 Size: 180 138 408 628 360 3544 Fragments arranged by size: 3544 628 408 360 180 138 RsrII CG'GwC_CG Cuts at: 3453 3453 Size: 5258 SacI G_AGCT'C Cuts at: 1784 2272 1784 Size: 488 4770 SalI G'TCGA_C Cuts at: 2295 2295 Size: 5258 SapI GCTCTTCn'nnn_ Cuts at: 1017 2120 3281 3491 1017 Size: 1103 1161 210 2784 Fragments arranged by size: 2784 1161 1103 210 SbfI CC_TGCA'GG Cuts at: 2305 2305 Size: 5258 ScaI AGT'ACT Cuts at: 1075 4775 1075 Size: 3700 1558 SfcI C'TryA_G Cuts at: 33 896 1741 2301 2986 3979 4170 33 Size: 863 845 560 685 993 191 1121 Fragments arranged by size: 1121 993 863 845 685 560 191 SfoI GGC'GCC Cuts at: 2690 2938 2690 Size: 248 5010 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5258 SmaI CCC'GGG Cuts at: 2280 2280 Size: 5258 SmlI C'TyrA_G Cuts at: 1519 2413 3328 3820 4082 1519 Size: 894 915 492 262 2695 Fragments arranged by size: 2695 915 894 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5258 SphI G_CATG'C Cuts at: 2311 2811 2311 Size: 500 4758 StyI C'CwwG_G Cuts at: 1676 2392 2746 1676 Size: 716 354 4188 Fragments arranged by size: 4188 716 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 130 2240 2841 2974 3433 3642 130 Size: 2110 601 133 459 209 1746 Fragments arranged by size: 2110 1746 601 459 209 133 TatI w'GTAC_w Cuts at: 853 1073 4773 853 Size: 220 3700 1338 Fragments arranged by size: 3700 1338 220 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4775 4807 4775 Size: 32 5226 Tth111I GACn'n_nGTC Cuts at: 1131 3055 1131 Size: 1924 3334 XbaI T'CTAG_A Cuts at: 2289 2289 Size: 5258 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1872 2753 1872 Size: 881 4377 XmaI C'CCGG_G Cuts at: 2278 2278 Size: 5258 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI NotI NruI NsiI PacI PasI PfoI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI