Restriction map for
pF1KE9251
(Circular) (Six-base) MAPSORT of: pF1KE9251.seq Check: 3343 from: 1 to: 4796
pF1KE9251 4796 bp
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1730 1730
Size: 4796
AccI GT'mk_AC
Cuts at: 758 1834 758
Size: 1076 3720
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 682 926 1247 1379 2621 3053 3800 4068
Size: 285 244 321 132 1242 432 747 268
Cuts at: 4068 397
Size: 1125
Fragments arranged by size:
1242 1125 747 432 321 285 268 244
132
AflIII A'CryG_T
Cuts at: 159 866 2148 3252 4229 159
Size: 707 1282 1104 977 726
Fragments arranged by size:
1282 1104 977 726 707
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AleI CACnn'nnGTG
Cuts at: 1204 1204
Size: 4796
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 4796
AlwNI CAG_nnn'CTG
Cuts at: 576 615 993 1375 1402 1677 2101 3668 576
Size: 39 378 382 27 275 424 1567 1704
Fragments arranged by size:
1704 1567 424 382 378 275 39 27
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 1800 4149 4405 1800
Size: 2349 256 2191
Fragments arranged by size:
2349 2191 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 209 517 1816 209
Size: 308 1299 3189
Fragments arranged by size:
3189 1299 308
AvrII C'CTAG_G
Cuts at: 1432 1432
Size: 4796
BamHI G'GATC_C
Cuts at: 1222 1821 1222
Size: 599 4197
BanI G'GyrC_C
Cuts at: 766 1718 1812 2226 2474 2509 766
Size: 952 94 414 248 35 3053
Fragments arranged by size:
3053 952 414 248 94 35
BanII G_rGCy'C
Cuts at: 222 231 518 524 1082 1810 2840 222
Size: 9 287 6 558 728 1030 2178
Fragments arranged by size:
2178 1030 728 558 287 9 6
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 914 1770 914
Size: 856 3940
BbvCI CC'TCA_GC
Cuts at: 1190 1238 1190
Size: 48 4748
BciVI GTATCCnnnnn_n'
Cuts at: 162 2694 3455 4796 162
Size: 2532 761 1341 162
Fragments arranged by size:
2532 1341 761 162
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 914 1908 914
Size: 994 3802
Bme1580I G_kGCm'C
Cuts at: 347 2421 2514 3570 347
Size: 2074 93 1056 1573
Fragments arranged by size:
2074 1573 1056 93
BmgBI CAC'GTC
Cuts at: 762 762
Size: 4796
BmrI ACTGGGnnnn_n'
Cuts at: 908 981 2008 2089 2424 4568 908
Size: 73 1027 81 335 2144 1136
Fragments arranged by size:
2144 1136 1027 335 81 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1296 1326 1559 3163 1296
Size: 30 233 1604 2929
Fragments arranged by size:
2929 1604 233 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 453 517 524 725 1082 1296 1315 1621
Size: 217 64 7 201 357 214 19 306
Cuts at: 1621 1672 1750 236
Size: 51 78 3282
Fragments arranged by size:
3282 357 306 217 214 201 78 64
51 19 7
Bpu10I CC'TnA_GC
Cuts at: 512 1039 1190 1238 512
Size: 527 151 48 4070
Fragments arranged by size:
4070 527 151 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 1378 1567 1972 2851 3343 3641 620
Size: 758 189 405 879 492 298 1775
Fragments arranged by size:
1775 879 758 492 405 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 4500 4500
Size: 4796
BsaAI yAC'GTr
Cuts at: 1704 2149 1704
Size: 445 4351
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 2227 2475 3177 4673 2227
Size: 248 702 1496 2350
Fragments arranged by size:
2350 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1985 2506 3184 3458 3605 4016 1985
Size: 521 678 274 147 411 2765
Fragments arranged by size:
2765 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 522 552 118
Size: 30 374 30 4362
Fragments arranged by size:
4362 374 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 794 1024 794
Size: 230 4566
BseYI C'CCAG_C
Cuts at: 830 1171 1368 2221 2979 3556 4052 830
Size: 341 197 853 758 577 496 1574
Fragments arranged by size:
1574 853 758 577 496 341 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 870 1684 733
Size: 137 814 3845
Fragments arranged by size:
3845 814 137
BsiEI CG_ry'CG
Cuts at: 104 930 2338 2384 3592 104
Size: 826 1408 46 1208 1308
Fragments arranged by size:
1408 1308 1208 826 46
BsiHKAI G_wGCw'C
Cuts at: 210 518 1045 1164 1810 2588 2778 3570 210
Size: 308 527 119 646 778 190 792 1436
Fragments arranged by size:
1436 792 778 646 527 308 190 119
BsmI GAATG_Cn'
Cuts at: 648 648
Size: 4796
Bsp1286I G_dGCh'C
Cuts at: 210 222 231 347 518 524 1045 1082 1164
Size: 12 9 116 171 6 521 37 82
Cuts at: 1164 1810 2421 2514 2588 2778 2840 3570 210
Size: 646 611 93 74 190 62 730 1436
Fragments arranged by size:
1436 730 646 611 521 190 171 116
93 82 74 62 37 12 9 6
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1730 1846 2060 2362 2743 1730
Size: 116 214 302 381 3783
Fragments arranged by size:
3783 381 302 214 116
BsrBI CCG'CTC
Cuts at: 50 1716 3088 3142 4626 4791 50
Size: 1666 1372 54 1484 165 55
Fragments arranged by size:
1666 1484 1372 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2708 2708
Size: 4796
BsrFI r'CCGG_y
Cuts at: 925 1447 1736 2794 2975 3184 925
Size: 522 289 1058 181 209 2537
Fragments arranged by size:
2537 1058 522 289 209 181
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 534 1480 534
Size: 946 3850
BstYI r'GATC_y
Cuts at: 1 667 780 1222 1467 1821 2288 2311 2646
Size: 666 113 442 245 354 467 23 335
Cuts at: 2646 2892 3904 3990 4002 4348 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 666 467 449 442 354 346 335
246 245 113 86 23 12
BtgI C'CryG_G
Cuts at: 107 190 297 848 1529 107
Size: 83 107 551 681 3374
Fragments arranged by size:
3374 681 551 107 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 951 1056 1696 2039 951
Size: 105 640 343 3708
Fragments arranged by size:
3708 640 343 105
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DraIII CAC_nnn'GTG
Cuts at: 1497 1497
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 264 1623 2381 2555 2946 2973 4318 264
Size: 1359 758 174 391 27 1345 742
Fragments arranged by size:
1359 1345 758 742 391 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 660 701 1094 1178 2819 3029 660
Size: 41 393 84 1641 210 2427
Fragments arranged by size:
2427 1641 393 210 84 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3004 3314 3460 4606 3004
Size: 310 146 1146 3194
Fragments arranged by size:
3194 1146 310 146
EcoICRI GAG'CTC
Cuts at: 516 1808 516
Size: 1292 3504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 397 453 517 524 682 725 926 1082
Size: 161 56 64 7 158 43 201 156
Cuts at: 1082 1247 1296 1315 1379 1621 1672 1750 2621
Size: 165 49 19 64 242 51 78 871
Cuts at: 2621 3053 3800 4068 236
Size: 432 747 268 964
Fragments arranged by size:
964 871 747 432 268 242 201 165
161 158 156 78 64 64 56 51
49 43 19 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1284 1284
Size: 4796
EcoO109I rG'GnC_Cy
Cuts at: 1168 1436 1457 1647 1674 1935 1168
Size: 268 21 190 27 261 4029
Fragments arranged by size:
4029 268 261 190 27 21
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 2577 2577
Size: 4796
HaeII r_GCGC'y
Cuts at: 1375 2230 2478 3500 4479 1375
Size: 855 248 1022 979 1692
Fragments arranged by size:
1692 1022 979 855 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 1678 1710 2638 2668 2670 2700 118
Size: 32 1528 32 928 30 2 30 2214
Fragments arranged by size:
2214 1528 928 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1835 1835
Size: 4796
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 159 4229 159
Size: 4070 726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 486 800 3467 3651 486
Size: 314 2667 184 1631
Fragments arranged by size:
2667 1631 314 184
MscI TGG'CCA
Cuts at: 266 2557 266
Size: 2291 2505
MslI CAynn'nnrTG
Cuts at: 415 1204 1589 2785 2912 415
Size: 789 385 1196 127 2299
Fragments arranged by size:
2299 1196 789 385 127
MspA1I CmG'CkG
Cuts at: 175 192 1743 1907 2221 2581 3594 3839 175
Size: 17 1551 164 314 360 1013 245 1132
Fragments arranged by size:
1551 1132 1013 360 314 245 164 17
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NcoI C'CATG_G
Cuts at: 107 297 848 1529 107
Size: 190 551 681 3374
Fragments arranged by size:
3374 681 551 190
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NspI r_CATG'y
Cuts at: 1849 2349 3256 1849
Size: 500 907 3389
Fragments arranged by size:
3389 907 500
PasI CC'CwG_GG
Cuts at: 973 1227 973
Size: 254 4542
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PflMI CCAn_nnn'nTGG
Cuts at: 993 1375 993
Size: 382 4414
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PmlI CAC'GTG
Cuts at: 1704 1704
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 1168 1647 1168
Size: 479 4317
PspXI vC'TCGA_Gb
Cuts at: 517 517
Size: 4796
PstI C_TGCA'G
Cuts at: 962 1386 1407 1843 2528 962
Size: 424 21 436 685 3230
Fragments arranged by size:
3230 685 436 424 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 1743 2221 2581 1743
Size: 478 360 3958
Fragments arranged by size:
3958 478 360
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 518 1810 518
Size: 1292 3504
SacII CC_GC'GG
Cuts at: 193 193
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 755 958 1382 1403 1839 2524 3517 3708
Size: 722 203 424 21 436 685 993 191
Cuts at: 3708 33
Size: 1121
Fragments arranged by size:
1121 993 722 685 436 424 203 191
21
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 517 635 1357 1546 1951 2866 3358 3620 517
Size: 118 722 189 405 915 492 262 1693
Fragments arranged by size:
1693 915 722 492 405 262 189 118
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
SspI AAT'ATT
Cuts at: 1576 1576
Size: 4796
StuI AGG'CCT
Cuts at: 720 720
Size: 4796
StyI C'CwwG_G
Cuts at: 107 297 848 1432 1529 1930 2284 107
Size: 190 551 584 97 401 354 2619
Fragments arranged by size:
2619 584 551 401 354 190 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 844 2379 2512 2971 3180 754
Size: 90 1535 133 459 209 2370
Fragments arranged by size:
2370 1535 459 209 133 90
TatI w'GTAC_w
Cuts at: 4311 4311
Size: 4796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 4313 4345 246
Size: 32 4035 32 697
Fragments arranged by size:
4035 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1690 2593 1690
Size: 903 3893
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 1527 2291 345
Size: 1182 764 2850
Fragments arranged by size:
2850 1182 764
XhoI C'TCGA_G
Cuts at: 517 517
Size: 4796
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI BaeI
BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspOMI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI