(Circular) (Six-base) MAPSORT of: pF1KE3491.seq Check: 7684 from: 1 to: 8708 pF1KE3491 8708 bp With 182 enzymes: SgfI * March 10, 2017 18:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2421 2421 Size: 8708 AatII G_ACGT'C Cuts at: 1595 1595 Size: 8708 AccI GT'mk_AC Cuts at: 1931 3344 5746 1931 Size: 1413 2402 4893 Fragments arranged by size: 4893 2402 1413 Acc65I G'GTAC_C Cuts at: 5724 5724 Size: 8708 AclI AA'CG_TT Cuts at: 1701 8548 1701 Size: 6847 1861 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 479 1003 2117 2184 2266 2738 2849 4727 5508 Size: 524 1114 67 82 472 111 1878 781 Cuts at: 5508 5599 5615 5629 6533 6965 7712 7980 479 Size: 91 16 14 904 432 747 268 1207 Fragments arranged by size: 1878 1207 1114 904 781 747 524 472 432 268 111 91 82 67 16 14 AfeI AGC'GCT Cuts at: 1957 1957 Size: 8708 AflII C'TTAA_G Cuts at: 2272 2272 Size: 8708 AflIII A'CryG_T Cuts at: 654 2684 6060 7164 8141 654 Size: 2030 3376 1104 977 1221 Fragments arranged by size: 3376 2030 1221 1104 977 AgeI A'CCGG_T Cuts at: 7096 7096 Size: 8708 AleI CACnn'nnGTG Cuts at: 643 643 Size: 8708 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4557 4589 5003 5035 4557 Size: 32 414 32 8230 Fragments arranged by size: 8230 414 32 32 AlwNI CAG_nnn'CTG Cuts at: 1410 4150 6013 7580 1410 Size: 2740 1863 1567 2538 Fragments arranged by size: 2740 2538 1863 1567 ApaLI G'TGCA_C Cuts at: 7478 7478 Size: 8708 ApoI r'AATT_y Cuts at: 209 393 541 929 1507 2373 2671 3670 3695 Size: 184 148 388 578 866 298 999 25 Cuts at: 3695 4132 4306 4414 5129 5284 5427 5655 5712 Size: 437 174 108 715 155 143 228 57 Cuts at: 5712 8061 8317 209 Size: 2349 256 600 Fragments arranged by size: 2349 999 866 715 600 578 437 388 298 256 228 184 174 155 148 143 108 57 25 AseI AT'TA_AT Cuts at: 20 917 1711 2995 20 Size: 897 794 1284 5733 Fragments arranged by size: 5733 1284 897 794 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8708 AvaI C'yCGr_G Cuts at: 5728 5728 Size: 8708 BamHI G'GATC_C Cuts at: 1417 5733 1417 Size: 4316 4392 BanI G'GyrC_C Cuts at: 4165 5724 6138 6386 6421 4165 Size: 1559 414 248 35 6452 Fragments arranged by size: 6452 1559 414 248 35 BanII G_rGCy'C Cuts at: 370 1139 5722 6752 370 Size: 769 4583 1030 2326 Fragments arranged by size: 4583 2326 1030 769 BbeI G_GCGC'C Cuts at: 6142 6390 6142 Size: 248 8460 BbsI GAAGACnn'nnnn_ Cuts at: 4631 4898 4631 Size: 267 8441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 686 720 686 Size: 34 8674 BciVI GTATCCnnnnn_n' Cuts at: 4749 6606 7367 8708 4749 Size: 1857 761 1341 4749 Fragments arranged by size: 4749 1857 1341 761 BclI T'GATC_A Cuts at: 6228 6228 Size: 8708 BfrBI ATG'CAT Cuts at: 1321 1503 2962 3256 1321 Size: 182 1459 294 6773 Fragments arranged by size: 6773 1459 294 182 BglII A'GATC_T Cuts at: 1074 2078 2816 3816 5574 6223 8260 1074 Size: 1004 738 1000 1758 649 2037 1522 Fragments arranged by size: 2037 1758 1522 1004 1000 738 649 BlpI GC'TnA_GC Cuts at: 5820 5820 Size: 8708 Bme1580I G_kGCm'C Cuts at: 6333 6426 7482 6333 Size: 93 1056 7559 Fragments arranged by size: 7559 1056 93 BmgBI CAC'GTC Cuts at: 3317 3317 Size: 8708 BmrI ACTGGGnnnn_n' Cuts at: 4077 5920 6001 6336 8480 4077 Size: 1843 81 335 2144 4305 Fragments arranged by size: 4305 2144 1843 335 81 BmtI G_CTAG'C Cuts at: 1084 3881 5356 1084 Size: 2797 1475 4436 Fragments arranged by size: 4436 2797 1475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 685 7075 685 Size: 6390 2318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 629 647 1598 1746 2274 4178 5300 629 Size: 18 951 148 528 1904 1122 4037 Fragments arranged by size: 4037 1904 1122 951 528 148 18 Bpu10I CC'TnA_GC Cuts at: 802 802 Size: 8708 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2456 4001 5884 6763 7255 7553 2456 Size: 1545 1883 879 492 298 3611 Fragments arranged by size: 3611 1883 1545 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4732 8412 4732 Size: 3680 5028 BsaAI yAC'GTr Cuts at: 6061 6061 Size: 8708 BsaBI GATnn'nnATC Cuts at: 6 1268 2480 5639 6222 8259 6 Size: 1262 1212 3159 583 2037 455 Fragments arranged by size: 3159 2037 1262 1212 583 455 BsaHI Gr'CG_yC Cuts at: 1592 6139 6387 7089 8585 1592 Size: 4547 248 702 1496 1715 Fragments arranged by size: 4547 1715 1496 702 248 BsaWI w'CCGG_w Cuts at: 4662 5897 6418 7096 7370 7517 7928 4662 Size: 1235 521 678 274 147 411 5442 Fragments arranged by size: 5442 1235 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 3743 3773 4412 4442 3743 Size: 30 639 30 8009 Fragments arranged by size: 8009 639 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 644 1763 3485 3996 4972 4982 644 Size: 1119 1722 511 976 10 4370 Fragments arranged by size: 4370 1722 1119 976 511 10 BseYI C'CCAG_C Cuts at: 2833 5490 6133 6891 7468 7964 2833 Size: 2657 643 758 577 496 3577 Fragments arranged by size: 3577 2657 758 643 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1283 1283 Size: 8708 BsiEI CG_ry'CG Cuts at: 104 6250 6296 7504 104 Size: 6146 46 1208 1308 Fragments arranged by size: 6146 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 5722 6500 6690 7482 5722 Size: 778 190 792 6948 Fragments arranged by size: 6948 792 778 190 BsmI GAATG_Cn' Cuts at: 831 3731 4814 831 Size: 2900 1083 4725 Fragments arranged by size: 4725 2900 1083 BsmBI CGTCTCn'nnnn_ Cuts at: 1583 1583 Size: 8708 Bsp1286I G_dGCh'C Cuts at: 370 1139 5722 6333 6426 6500 6690 6752 7482 Size: 769 4583 611 93 74 190 62 730 Cuts at: 7482 370 Size: 1596 Fragments arranged by size: 4583 1596 769 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 7928 7928 Size: 8708 BspHI T'CATG_A Cuts at: 1791 3180 3474 3618 4632 7884 1791 Size: 1389 294 144 1014 3252 2615 Fragments arranged by size: 3252 2615 1389 1014 294 144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2001 2275 2421 4119 5758 5972 6274 6655 2001 Size: 274 146 1698 1639 214 302 381 4054 Fragments arranged by size: 4054 1698 1639 381 302 274 214 146 BsrBI CCG'CTC Cuts at: 50 7000 7054 8538 8703 50 Size: 6950 54 1484 165 55 Fragments arranged by size: 6950 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1370 6620 1370 Size: 5250 3458 BsrFI r'CCGG_y Cuts at: 6706 6887 7096 6706 Size: 181 209 8318 Fragments arranged by size: 8318 209 181 BsrGI T'GTAC_A Cuts at: 522 2320 2733 3354 522 Size: 1798 413 621 5876 Fragments arranged by size: 5876 1798 621 413 BssSI C'ACGA_G Cuts at: 3902 6979 7337 3902 Size: 3077 358 5273 Fragments arranged by size: 5273 3077 358 BstAPI GCAn_nnn'nTGC Cuts at: 280 477 2068 6085 280 Size: 197 1591 4017 2903 Fragments arranged by size: 4017 2903 1591 197 BstBI TT'CG_AA Cuts at: 7069 7069 Size: 8708 BstEII G'GTnAC_C Cuts at: 310 310 Size: 8708 BstXI CCAn_nnnn'nTGG Cuts at: 3927 3927 Size: 8708 BstYI r'GATC_y Cuts at: 1 1074 1417 2078 2534 2816 3816 4077 5467 Size: 1073 343 661 456 282 1000 261 1390 Cuts at: 5467 5574 5733 6200 6223 6558 6804 7816 7902 Size: 107 159 467 23 335 246 1012 86 Cuts at: 7902 7914 8260 1 Size: 12 346 449 Fragments arranged by size: 1390 1073 1012 1000 661 467 456 449 346 343 335 282 261 246 159 107 86 23 12 Bsu36I CC'TnA_GG Cuts at: 5618 5618 Size: 8708 BtgI C'CryG_G Cuts at: 724 2216 2540 724 Size: 1492 324 6892 Fragments arranged by size: 6892 1492 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 6658 6839 7002 6658 Size: 181 163 8364 Fragments arranged by size: 8364 181 163 BtsI GCAGTG_nn' Cuts at: 1300 1411 3640 3854 5951 1300 Size: 111 2229 214 2097 4057 Fragments arranged by size: 4057 2229 2097 214 111 DraI TTT'AAA Cuts at: 2553 4464 5707 2553 Size: 1911 1243 5554 Fragments arranged by size: 5554 1911 1243 DrdI GACnn_nn'nnGTC Cuts at: 6414 7272 6414 Size: 858 7850 EaeI y'GGCC_r Cuts at: 945 1176 1749 2174 3098 3804 6293 6467 6858 Size: 231 573 425 924 706 2489 174 391 Cuts at: 6858 6885 8230 945 Size: 27 1345 1423 Fragments arranged by size: 2489 1423 1345 924 706 573 425 391 231 174 27 EagI C'GGCC_G Cuts at: 6293 6293 Size: 8708 EarI CTCTTCn'nnn_ Cuts at: 891 1999 2525 3623 3683 4016 4164 4865 4974 Size: 1108 526 1098 60 333 148 701 109 Cuts at: 4974 5336 5575 6731 6941 891 Size: 362 239 1156 210 2658 Fragments arranged by size: 2658 1156 1108 1098 701 526 362 333 239 210 148 109 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 895 6916 7226 7372 8518 895 Size: 6021 310 146 1146 1085 Fragments arranged by size: 6021 1146 1085 310 146 EcoICRI GAG'CTC Cuts at: 5720 5720 Size: 8708 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 479 629 647 1003 1598 1746 2117 2184 2266 Size: 150 18 356 595 148 371 67 82 Cuts at: 2266 2274 2738 2849 4178 4727 5300 5508 5599 Size: 8 464 111 1329 549 573 208 91 Cuts at: 5599 5615 5629 6533 6965 7712 7980 479 Size: 16 14 904 432 747 268 1207 Fragments arranged by size: 1329 1207 904 747 595 573 549 464 432 371 356 268 208 150 148 111 91 82 67 18 16 14 8 EcoNI CCTnn'n_nnAGG Cuts at: 1692 4838 1692 Size: 3146 5562 EcoO109I rG'GnC_Cy Cuts at: 180 3067 5847 180 Size: 2887 2780 3041 Fragments arranged by size: 3041 2887 2780 EcoRI G'AATT_C Cuts at: 929 3695 5712 929 Size: 2766 2017 3925 Fragments arranged by size: 3925 2766 2017 EcoRV GAT'ATC Cuts at: 5019 5019 Size: 8708 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1453 4042 6727 1453 Size: 2589 2685 3434 Fragments arranged by size: 3434 2685 2589 FspI TGC'GCA Cuts at: 6489 6489 Size: 8708 HaeII r_GCGC'y Cuts at: 1959 6142 6390 7412 8391 1959 Size: 4183 248 1022 979 2276 Fragments arranged by size: 4183 2276 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1610 1642 3114 3146 3743 3775 4669 4701 6550 Size: 32 1472 32 597 32 894 32 1849 Cuts at: 6550 6580 6582 6612 1610 Size: 30 2 30 3706 Fragments arranged by size: 3706 1849 1472 894 597 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1368 3323 3762 5747 1368 Size: 1955 439 1985 4329 Fragments arranged by size: 4329 1985 1955 439 HindIII A'AGCT_T Cuts at: 2620 5155 5560 2620 Size: 2535 405 5768 Fragments arranged by size: 5768 2535 405 KasI G'GCGC_C Cuts at: 6138 6386 6138 Size: 248 8460 KpnI G_GTAC'C Cuts at: 5728 5728 Size: 8708 MfeI C'AATT_G Cuts at: 2663 2894 2663 Size: 231 8477 MluI A'CGCG_T Cuts at: 8141 8141 Size: 8708 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 639 846 1395 1582 3729 3851 5350 7379 7563 Size: 207 549 187 2147 122 1499 2029 184 Cuts at: 7563 639 Size: 1784 Fragments arranged by size: 2147 2029 1784 1499 549 207 187 184 122 MscI TGG'CCA Cuts at: 947 1178 3100 3806 6469 947 Size: 231 1922 706 2663 3186 Fragments arranged by size: 3186 2663 1922 706 231 MslI CAynn'nnrTG Cuts at: 643 847 1353 2221 2545 3118 6697 6824 643 Size: 204 506 868 324 573 3579 127 2527 Fragments arranged by size: 3579 2527 868 573 506 324 204 127 MspA1I CmG'CkG Cuts at: 4777 5819 6133 6493 7506 7751 4777 Size: 1042 314 360 1013 245 5734 Fragments arranged by size: 5734 1042 1013 360 314 245 NaeI GCC'GGC Cuts at: 6889 6889 Size: 8708 NarI GG'CG_CC Cuts at: 6139 6387 6139 Size: 248 8460 NcoI C'CATG_G Cuts at: 724 2216 2540 724 Size: 1492 324 6892 Fragments arranged by size: 6892 1492 324 NdeI CA'TA_TG Cuts at: 444 2097 2964 3541 444 Size: 1653 867 577 5611 Fragments arranged by size: 5611 1653 867 577 NgoMIV G'CCGG_C Cuts at: 6887 6887 Size: 8708 NheI G'CTAG_C Cuts at: 1080 3877 5352 1080 Size: 2797 1475 4436 Fragments arranged by size: 4436 2797 1475 NsiI A_TGCA'T Cuts at: 1323 1505 2964 3258 1323 Size: 182 1459 294 6773 Fragments arranged by size: 6773 1459 294 182 NspI r_CATG'y Cuts at: 262 537 658 682 1503 4905 5761 6261 7168 Size: 275 121 24 821 3402 856 500 907 Cuts at: 7168 262 Size: 1802 Fragments arranged by size: 3402 1802 907 856 821 500 275 121 24 PciI A'CATG_T Cuts at: 654 7164 654 Size: 6510 2198 PflMI CCAn_nnn'nTGG Cuts at: 4150 4150 Size: 8708 PfoI T'CCnGG_A Cuts at: 121 198 1614 121 Size: 77 1416 7215 Fragments arranged by size: 7215 1416 77 PmeI GTTT'AAAC Cuts at: 5707 5707 Size: 8708 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7847 7879 7847 Size: 32 8676 PpuMI rG'GwC_Cy Cuts at: 180 3067 180 Size: 2887 5821 PsiI TTA'TAA Cuts at: 5093 5093 Size: 8708 PstI C_TGCA'G Cuts at: 600 1074 4116 5755 6440 600 Size: 474 3042 1639 685 2868 Fragments arranged by size: 3042 2868 1639 685 474 PvuI CG_AT'CG Cuts at: 104 104 Size: 8708 PvuII CAG'CTG Cuts at: 4777 6133 6493 4777 Size: 1356 360 6992 Fragments arranged by size: 6992 1356 360 RsrII CG'GwC_CG Cuts at: 6903 6903 Size: 8708 SacI G_AGCT'C Cuts at: 5722 5722 Size: 8708 SalI G'TCGA_C Cuts at: 5745 5745 Size: 8708 SapI GCTCTTCn'nnn_ Cuts at: 3623 4974 6731 6941 3623 Size: 1351 1757 210 5390 Fragments arranged by size: 5390 1757 1351 210 SbfI CC_TGCA'GG Cuts at: 5755 5755 Size: 8708 ScaI AGT'ACT Cuts at: 8225 8225 Size: 8708 SexAI A'CCwGG_T Cuts at: 2117 5002 2117 Size: 2885 5823 SfcI C'TryA_G Cuts at: 33 417 596 1070 1677 4112 4394 5553 5751 Size: 384 179 474 607 2435 282 1159 198 Cuts at: 5751 6436 7429 7620 33 Size: 685 993 191 1121 Fragments arranged by size: 2435 1159 1121 993 685 607 474 384 282 198 191 179 SfoI GGC'GCC Cuts at: 6140 6388 6140 Size: 248 8460 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8708 SmaI CCC'GGG Cuts at: 5730 5730 Size: 8708 SmlI C'TyrA_G Cuts at: 2272 2471 4016 5863 6778 7270 7532 2272 Size: 199 1545 1847 915 492 262 3448 Fragments arranged by size: 3448 1847 1545 915 492 262 199 SpeI A'CTAG_T Cuts at: 67 67 Size: 8708 SphI G_CATG'C Cuts at: 537 1503 5761 6261 537 Size: 966 4258 500 2984 Fragments arranged by size: 4258 2984 966 500 SspI AAT'ATT Cuts at: 3017 3432 4056 4238 3017 Size: 415 624 182 7487 Fragments arranged by size: 7487 624 415 182 StuI AGG'CCT Cuts at: 4925 4925 Size: 8708 StyI C'CwwG_G Cuts at: 176 724 1421 2216 2540 5206 5842 6196 176 Size: 548 697 795 324 2666 636 354 2688 Fragments arranged by size: 2688 2666 795 697 636 548 354 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 298 569 3239 4896 6291 6424 6883 7092 298 Size: 271 2670 1657 1395 133 459 209 1914 Fragments arranged by size: 2670 1914 1657 1395 459 271 209 133 TatI w'GTAC_w Cuts at: 522 2320 2733 3354 4318 4992 8223 522 Size: 1798 413 621 964 674 3231 1007 Fragments arranged by size: 3231 1798 1007 964 674 621 413 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3080 3112 3712 3744 4552 4584 8225 8257 3080 Size: 32 600 32 808 32 3641 32 3531 Fragments arranged by size: 3641 3531 808 600 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 6505 6505 Size: 8708 XbaI T'CTAG_A Cuts at: 5739 5739 Size: 8708 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1524 4354 6203 1524 Size: 2830 1849 4029 Fragments arranged by size: 4029 2830 1849 XmaI C'CCGG_G Cuts at: 5728 5728 Size: 8708 XmnI GAAnn'nnTTC Cuts at: 464 1016 1190 464 Size: 552 174 7982 Fragments arranged by size: 7982 552 174 ZraI GAC'GTC Cuts at: 1593 1593 Size: 8708 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AhdI AlfI ApaI AscI AvrII BaeI BbvCI BglI BsiWI BssHII BstZ17I ClaI CspCI DraIII FseI FspAI HpaI NotI NruI PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI