Restriction map for pF1KE2413
##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

AclI        AflII       Aor51HI     AscI        AseI        
AsnI        BfrI        BlpI        Bpu1102I    BsiEI       
BsiWI       BspLU11I    BssHII      BstBI       CelII       
CpoI        DraI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       FseI        HpaI        
MfeI        MluI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SfuI        SgrAI       SpeI        SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1346  GACGTC               GACGT^C
AgeI         1423  ACCGGT               A^CCGGT
AhdI         5860  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        873  GTGCAC               G^TGCAC
ApaLI         873  GTGCAC               G^TGCAC
AspEI        5860  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3208  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        5615  TGCGCA               TGC^GCA
AvrII         132  CCTAGG               C^CTAGG
BbeI         1840  GGCGCC               GGCGC^C
BbrPI        5635  CACGTG               CAC^GTG
BciVI        4463  GTATCC               GTATCC(6/5)
BlnI          132  CCTAGG               C^CTAGG
Bsp1407I     1483  TGTACA               T^GTACA
BspDI        3286  ATCGAT               AT^CGAT
BsrBI        3175  GAGCGG               CCGCTC(-3/-3)
BsrFI        1423  ACCGGT               [GA]^CCGG[CT]
BsrGI        1483  TGTACA               T^GTACA
BssSI         727  CTCGTG               CACGAG(-5/-1)
Bst1107I     3136  GTATAC               GTA^TAC
BstZ17I      3136  GTATAC               GTA^TAC
Cfr10I       1423  ACCGGT               [GA]^CCGG[CT]
ClaI         3286  ATCGAT               AT^CGAT
Eam1105I     5860  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         378  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        4381  GAATTC               G^AATTC
EcoT22I      3290  ATGCAT               ATGCA^T
FspI         5615  TGCGCA               TGC^GCA
HindIII      3913  AAGCTT               A^AGCTT
KasI         1840  GGCGCC               G^GCGCC
NarI         1840  GGCGCC               GG^CGCC
NsiI         3290  ATGCAT               ATGCA^T
PflFI        3208  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1423  ACCGGT               A^CCGGT
PmaCI        5635  CACGTG               CAC^GTG
PmlI         5635  CACGTG               CAC^GTG
PshAI        4876  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        5351  ACCTGGT              A^CC[AT]GGT
SfiI         1294  GGCCGGGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1840  GGCGCC               GGC^GCC
SmaI           88  CCCGGG               CCC^GGG
SnaBI         394  TACGTA               TAC^GTA
SspBI        1483  TGTACA               T^GTACA
Tth111I      3208  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          88  CCCGGG               C^CCGGG
XmaI           88  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         3136  GTATAC               GT^[AC][GT]AC
             3355  GTCTAC              
AccIII       2990  TCCGGA               T^CCGGA
             4592  TCCGGA              
Asp700I       273  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3387  GAACATCTTC          
BclI         4292  TGATCA               T^GATCA
             4316  TGATCA              
BglII        3335  AGATCT               A^GATCT
             4667  AGATCT              
BsaAI         394  TACGTA               [CT]AC^GT[GA]
             5635  CACGTG              
BsaBI        3384  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4653  GATTGTCATC          
BseAI        2990  TCCGGA               T^CCGGA
             4592  TCCGGA              
BsmI          785  GAATGC               GAATGC(1/-1)
             3115  GAATGC              
BspEI        2990  TCCGGA               T^CCGGA
             4592  TCCGGA              
DraIII        670  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4501  CACCGAGTG           
FbaI         4292  TGATCA               T^GATCA
             4316  TGATCA              
HincII       1629  GTTGAC               GT[CT]^[GA]AC
             3187  GTCAAC              
HindII       1629  GTTGAC               GT[CT]^[GA]AC
             3187  GTCAAC              
KspI          897  CCGCGG               CCGC^GG
             2793  CCGCGG              
MamI         3384  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4653  GATTGTCATC          
MroI         2990  TCCGGA               T^CCGGA
             4592  TCCGGA              
MseI         1898  TTAA                 T^TAA
             2819  TTAA                
NdeI         2168  CATATG               CA^TATG
             3293  CATATG              
PaeR7I        766  CTCGAG               C^TCGAG
             5735  CTCGAG              
SacI         3846  GAGCTC               GAGCT^C
             5327  GAGCTC              
SacII         897  CCGCGG               CCGC^GG
             2793  CCGCGG              
ScaI         2907  AGTACT               AGT^ACT
             2916  AGTACT              
SphI         2021  GCATGC               GCATG^C
             4034  GCATGC              
Tru9I        1898  TTAA                 T^TAA
             2819  TTAA                
XhoI          766  CTCGAG               C^TCGAG
             5735  CTCGAG              
XmnI          273  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3387  GAACATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  138 enzymes) #
#########################################

Acc65I      AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
AocI        ApaI        ApoI        Asp718I     AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrDI       BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KpnI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SapI        Sau3AI      Sau96I      
SbfI        ScrFI       SfaNI       SfcI        SmlI        
Sse8387I    StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       
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