Restriction map for pF1KB9166
 (Circular) (Six-base) MAPSORT of: pF1KB9166.seq  Check: 4253  from: 1  to: 9122



pF1KB9166  9122 bp

 With 182 enzymes: SgfI * 

                        October 29, 2012 16:51  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    235     859    1012    1108    1222    1399    2113    2263    2377 
   Size:        624     153      96     114     177     714     150     114

Cuts at:   2377    2947    3364     235 
   Size:        570     417    5993

  Fragments arranged by size: 

                5993     714     624     570     417     177     153     150
                 114     114      96
 
AatII G_ACGT'C

Cuts at:   2798    5525    2798 
   Size:       2727    6395
 
AccI GT'mk_AC

Cuts at:   2831    4527    5759    6160    2831 
   Size:       1696    1232     401    5793

  Fragments arranged by size: 

                5793    1696    1232     401
 
Acc65I G'GTAC_C

Cuts at:   2446    6138    2446 
   Size:       3692    5430
 
AclI AA'CG_TT

Cuts at:   8962    8962 
   Size:       9122
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   3833    3986    4169    4669    4748    6947    7379    8126    8394 
   Size:        153     183     500      79    2199     432     747     268

Cuts at:   8394    3833 
   Size:       4561

  Fragments arranged by size: 

                4561    2199     747     500     432     268     183     153
                  79
 
AflIII A'CryG_T

Cuts at:   1289    6474    7578    8555    1289 
   Size:       5185    1104     977    1856

  Fragments arranged by size: 

                5185    1856    1104     977
 
AgeI A'CCGG_T

Cuts at:   7510    7510 
   Size:       9122
 
AhdI GACnn_n'nnGTC

Cuts at:   2303    2303 
   Size:       9122
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    834    1741    4061    5045    5345     834 
   Size:        907    2320     984     300    4611

  Fragments arranged by size: 

                4611    2320     984     907     300
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3042    3074    3042 
   Size:         32    9090
 
AlwNI CAG_nnn'CTG

Cuts at:   1404    2549    2637    3166    3210    4041    4215    4699    5163 
   Size:       1145      88     529      44     831     174     484     464

Cuts at:   5163    5463    6427    7994    1404 
   Size:        300     964    1567    2532

  Fragments arranged by size: 

                2532    1567    1145     964     831     529     484     464
                 300     174      88      44
 
ApaI G_GGCC'C

Cuts at:   1388    2393    2506    2699    3372    3571    3611    3992    4143 
   Size:       1005     113     193     673     199      40     381     151

Cuts at:   4143    5624    1388 
   Size:       1481    4886

  Fragments arranged by size: 

                4886    1481    1005     673     381     199     193     151
                 113      40
 
ApaLI G'TGCA_C

Cuts at:    414    7892     414 
   Size:       7478    1644
 
ApoI r'AATT_y

Cuts at:   6126    8475    8731    6126 
   Size:       2349     256    6517

  Fragments arranged by size: 

                6517    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       9122
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9122
 
AvaI C'yCGr_G

Cuts at:    519    1203    3687    4055    4142    4169    5144    5348    5483 
   Size:        684    2484     368      87      27     975     204     135

Cuts at:   5483    5808    6142     519 
   Size:        325     334    3499

  Fragments arranged by size: 

                3499    2484     975     684     368     334     325     204
                 135      87      27
 
AvrII C'CTAG_G

Cuts at:    369     826    4491     369 
   Size:        457    3665    5000

  Fragments arranged by size: 

                5000    3665     457
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2438    2471    2438 
   Size:         33    9089
 
BamHI G'GATC_C

Cuts at:   1207    6147    1207 
   Size:       4940    4182
 
BanI G'GyrC_C

Cuts at:    223     730     847    1096    1741    2446    2467    6138    6552 
   Size:        507     117     249     645     705      21    3671     414

Cuts at:   6552    6800    6835     223 
   Size:        248      35    2510

  Fragments arranged by size: 

                3671    2510     705     645     507     414     249     248
                 117      35      21
 
BanII G_rGCy'C

Cuts at:    377     757    1234    1388    2393    2506    2699    3372    3571 
   Size:        380     477     154    1005     113     193     673     199

Cuts at:   3571    3611    3992    4021    4143    4368    4376    4500    4548 
   Size:         40     381      29     122     225       8     124      48

Cuts at:   4548    4952    5624    5679    6136    7166     377 
   Size:        404     672      55     457    1030    2333

  Fragments arranged by size: 

                2333    1030    1005     673     672     477     457     404
                 381     380     225     199     193     154     124     122
                 113      55      48      40      29       8
 
BbeI G_GCGC'C

Cuts at:   6556    6804    6556 
   Size:        248    8874
 
BbsI GAAGACnn'nnnn_

Cuts at:    470    1292    5255     470 
   Size:        822    3963    4337

  Fragments arranged by size: 

                4337    3963     822
 
BbvCI CC'TCA_GC

Cuts at:   1662    1662 
   Size:       9122
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     655     689    3569    3603    5371    5405      90 
   Size:         34     531      34    2880      34    1768      34    3807

  Fragments arranged by size: 

                3807    2880    1768     531      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   7020    7781    9122    7020 
   Size:        761    1341    7020

  Fragments arranged by size: 

                7020    1341     761
 
BclI T'GATC_A

Cuts at:   1530    3888    6642    1530 
   Size:       2358    2754    4010

  Fragments arranged by size: 

                4010    2754    2358
 
BglI GCCn_nnn'nGGC

Cuts at:    428     659     728    1616    2264    2354    2609    3095    3461 
   Size:        231      69     888     648      90     255     486     366

Cuts at:   3461    4495    5239     428 
   Size:       1034     744    4311

  Fragments arranged by size: 

                4311    1034     888     744     648     486     366     255
                 231      90      69
 
BglII A'GATC_T

Cuts at:   6637    8674    6637 
   Size:       2037    7085
 
BlpI GC'TnA_GC

Cuts at:   6234    6234 
   Size:       9122
 
Bme1580I G_kGCm'C

Cuts at:    270     418     850     976    1099    1203    1388    2085    2120 
   Size:        148     432     126     123     104     185     697      35

Cuts at:   2120    2211    2393    2506    2655    2699    2979    3372    3571 
   Size:         91     182     113     149      44     280     393     199

Cuts at:   3571    3611    3908    3992    4143    5005    5624    5660    6747 
   Size:         40     297      84     151     862     619      36    1087

Cuts at:   6747    6840    7896     270 
   Size:         93    1056    1496

  Fragments arranged by size: 

                1496    1087    1056     862     697     619     432     393
                 297     280     199     185     182     151     149     148
                 126     123     113     104      93      91      84      44
                  40      36      35
 
BmgBI CAC'GTC

Cuts at:    290    2148    3126    5286     290 
   Size:       1858     978    2160    4126

  Fragments arranged by size: 

                4126    2160    1858     978
 
BmrI ACTGGGnnnn_n'

Cuts at:   2388    2702    2948    3708    4459    5015    5334    5553    6334 
   Size:        314     246     760     751     556     319     219     781

Cuts at:   6334    6415    6750    8894    2388 
   Size:         81     335    2144    2616

  Fragments arranged by size: 

                2616    2144     781     760     751     556     335     319
                 314     246     219      81
 
BmtI G_CTAG'C

Cuts at:   5512    5512 
   Size:       9122
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    257     891    1563    1677    4248    7489     257 
   Size:        634     672     114    2571    3241    1890

  Fragments arranged by size: 

                3241    2571    1890     672     634     114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1838    2372    2671    2704    3077    3089    3587    3836    3848 
   Size:        534     299      33     373      12     498     249      12

Cuts at:   3848    4390    4495    5096    5353    1838 
   Size:        542     105     601     257    5607

  Fragments arranged by size: 

                5607     601     542     534     498     373     299     257
                 249     105      33      12      12
 
Bpu10I CC'TnA_GC

Cuts at:    232    1662    3425    4692    4946     232 
   Size:       1430    1763    1267     254    4408

  Fragments arranged by size: 

                4408    1763    1430    1267     254
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4624    5089    6298    7177    7669    7967    4624 
   Size:        465    1209     879     492     298    5779

  Fragments arranged by size: 

                5779    1209     879     492     465     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1639    3544    3838    4219    4642    4769    5005    5943    8826 
   Size:       1905     294     381     423     127     236     938    2883

Cuts at:   8826    1639 
   Size:       1935

  Fragments arranged by size: 

                2883    1935    1905     938     423     381     294     236
                 127
 
BsaAI yAC'GTr

Cuts at:   2898    4751    6475    2898 
   Size:       1853    1724    5545

  Fragments arranged by size: 

                5545    1853    1724
 
BsaBI GATnn'nnATC

Cuts at:      6    6636    8673       6 
   Size:       6630    2037     455

  Fragments arranged by size: 

                6630    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2795    4524    4530    4605    5413    5522    6553    6801    7503 
   Size:       1729       6      75     808     109    1031     248     702

Cuts at:   7503    8999    2795 
   Size:       1496    2918

  Fragments arranged by size: 

                2918    1729    1496    1031     808     702     248     109
                  75       6
 
BsaWI w'CCGG_w

Cuts at:   2617    6311    6832    7510    7784    7931    8342    2617 
   Size:       3694     521     678     274     147     411    3397

  Fragments arranged by size: 

                3694    3397     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1498    1528    2358    2388    3042    3072    4700    4730    5921 
   Size:         30     830      30     654      30    1628      30    1191

Cuts at:   5921    5951    1498 
   Size:         30    4669

  Fragments arranged by size: 

                4669    1628    1191     830     654      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1180    1768    2305    4717    4726    4802    5840    6116    1180 
   Size:        588     537    2412       9      76    1038     276    4186

  Fragments arranged by size: 

                4186    2412    1038     588     537     276      76       9
            
 
BseYI C'CCAG_C

Cuts at:    320     379     403     455    1031    1145    1424    1550    2231 
   Size:         59      24      52     576     114     279     126     681

Cuts at:   2231    2530    2948    3941    4023    4243    5456    5466    5472 
   Size:        299     418     993      82     220    1213      10       6

Cuts at:   5472    5503    5572    5617    6069    6547    7305    7882    8378 
   Size:         31      69      45     452     478     758     577     496

Cuts at:   8378     320 
   Size:       1064

  Fragments arranged by size: 

                1213    1064     993     758     681     577     576     496
                 478     452     418     299     279     220     126     114
                  82      69      59      52      45      31      24      10
                   6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1791    2085    2183    2235    2349    2558    2759    3843    5005 
   Size:        294      98      52     114     209     201    1084    1162

Cuts at:   5005    5305    1791 
   Size:        300    5608

  Fragments arranged by size: 

                5608    1162    1084     300     294     209     201     114
                  98      52
 
BsiEI CG_ry'CG

Cuts at:    104    5847    6664    6710    7918     104 
   Size:       5743     817      46    1208    1308

  Fragments arranged by size: 

                5743    1308    1208     817      46
 
BsiHKAI G_wGCw'C

Cuts at:    418     538    1998    4462    4548    4693    6136    6914    7104 
   Size:        120    1460    2464      86     145    1443     778     190

Cuts at:   7104    7896     418 
   Size:        792    1644

  Fragments arranged by size: 

                2464    1644    1460    1443     792     778     190     145
                 120      86
 
BsmBI CGTCTCn'nnnn_

Cuts at:    280    2802    3116     280 
   Size:       2522     314    6286

  Fragments arranged by size: 

                6286    2522     314
 
Bsp1286I G_dGCh'C

Cuts at:    270     377     418     538     757     850     976    1099    1203 
   Size:        107      41     120     219      93     126     123     104

Cuts at:   1203    1234    1388    1998    2085    2120    2211    2393    2506 
   Size:         31     154     610      87      35      91     182     113

Cuts at:   2506    2655    2699    2979    3372    3571    3611    3908    3992 
   Size:        149      44     280     393     199      40     297      84

Cuts at:   3992    4021    4143    4368    4376    4462    4500    4548    4693 
   Size:         29     122     225       8      86      38      48     145

Cuts at:   4693    4952    5005    5624    5660    5679    6136    6747    6840 
   Size:        259      53     619      36      19     457     611      93

Cuts at:   6840    6914    7104    7166    7896     270 
   Size:         74     190      62     730    1496

  Fragments arranged by size: 

                1496     730     619     611     610     457     393     297
                 280     259     225     219     199     190     182     154
                 149     145     126     123     122     120     113     107
                 104      93      93      91      87      86      84      74
                  62      53      48      44      41      40      38      36
                  35      31      29      19       8
 
BspEI T'CCGG_A

Cuts at:   8342    8342 
   Size:       9122
 
BspHI T'CATG_A

Cuts at:   8298    8298 
   Size:       9122
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    235     265     859    1012    1108    1222    1399    1706    2113 
   Size:         30     594     153      96     114     177     307     407

Cuts at:   2113    2230    2263    2377    2575    2947    3364    6172    6386 
   Size:        117      33     114     198     372     417    2808     214

Cuts at:   6386    6688    7069     235 
   Size:        302     381    2288

  Fragments arranged by size: 

                2808    2288     594     417     407     381     372     307
                 302     214     198     177     153     117     114     114
                  96      33      30
 
BsrBI CCG'CTC

Cuts at:     50     364    5360    5443    6039    7414    7468    8952    9117 
   Size:        314    4996      83     596    1375      54    1484     165

Cuts at:   9117      50 
   Size:         55

  Fragments arranged by size: 

                4996    1484    1375     596     314     165      83      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   5793    7034    5793 
   Size:       1241    7881
 
BsrFI r'CCGG_y

Cuts at:   4607    5514    7120    7301    7510    4607 
   Size:        907    1606     181     209    6219

  Fragments arranged by size: 

                6219    1606     907     209     181
 
BssHII G'CGCG_C

Cuts at:   5631    5631 
   Size:       9122
 
BssSI C'ACGA_G

Cuts at:   5717    7393    7751    5717 
   Size:       1676     358    7088

  Fragments arranged by size: 

                7088    1676     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    737    2206    2885    4429    6499     737 
   Size:       1469     679    1544    2070    3360

  Fragments arranged by size: 

                3360    2070    1544    1469     679
 
BstBI TT'CG_AA

Cuts at:    838    7483     838 
   Size:       6645    2477
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1022    2239    2943    2956    3561    3737    1022 
   Size:       1217     704      13     605     176    6407

  Fragments arranged by size: 

                6407    1217     704     605     176      13
 
BstYI r'GATC_y

Cuts at:      1     638    1207    2128    5547    6147    6614    6637    6972 
   Size:        637     569     921    3419     600     467      23     335

Cuts at:   6972    7218    8230    8316    8328    8674       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                3419    1012     921     637     600     569     467     449
                 346     335     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2090    2582    4700    4793    2090 
   Size:        492    2118      93    6419

  Fragments arranged by size: 

                6419    2118     492      93
 
BtgI C'CryG_G

Cuts at:   1311    2112    2211    2489    3045    3399    3554    4854    4997 
   Size:        801      99     278     556     354     155    1300     143

Cuts at:   4997    1311 
   Size:       5436

  Fragments arranged by size: 

                5436    1300     801     556     354     278     155     143
                  99
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3683    4363    7072    7253    7416    3683 
   Size:        680    2709     181     163    5389

  Fragments arranged by size: 

                5389    2709     680     181     163
 
BtsI GCAGTG_nn'

Cuts at:    121     571     648    1080    1125    1599    1807    1827    2118 
   Size:        450      77     432      45     474     208      20     291

Cuts at:   2118    2679    2706    2856    3164    3327    3390    3687    3796 
   Size:        561      27     150     308     163      63     297     109

Cuts at:   3796    3926    4364    4390    5192    6365     121 
   Size:        130     438      26     802    1173    2878

  Fragments arranged by size: 

                2878    1173     802     561     474     450     438     432
                 308     297     291     208     163     150     130     109
                  77      63      45      27      26      20
 
ClaI AT'CG_AT

Cuts at:   1763    1763 
   Size:       9122
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1200    1235    1669    1704    1200 
   Size:         35     434      35    8618

  Fragments arranged by size: 

                8618     434      35      35
 
DraI TTT'AAA

Cuts at:   6121    6121 
   Size:       9122
 
DraIII CAC_nnn'GTG

Cuts at:    799    1131    2206    3448    3805    4998     799 
   Size:        332    1075    1242     357    1193    4923

  Fragments arranged by size: 

                4923    1242    1193    1075     357     332
 
DrdI GACnn_nn'nnGTC

Cuts at:    946    6828    7686     946 
   Size:       5882     858    2382

  Fragments arranged by size: 

                5882    2382     858
 
EaeI y'GGCC_r

Cuts at:    623    3185    4469    5044    5844    6707    6881    7272    7299 
   Size:       2562    1284     575     800     863     174     391      27

Cuts at:   7299    8644     623 
   Size:       1345    1101

  Fragments arranged by size: 

                2562    1345    1284    1101     863     800     575     391
                 174      27
 
EagI C'GGCC_G

Cuts at:   5844    6707    5844 
   Size:        863    8259
 
EarI CTCTTCn'nnn_

Cuts at:   2805    3781    3809    4505    7145    7355    2805 
   Size:        976      28     696    2640     210    4572

  Fragments arranged by size: 

                4572    2640     976     696     210      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2515    5533    5741    7330    7640    7786    8932    2515 
   Size:       3018     208    1589     310     146    1146    2705

  Fragments arranged by size: 

                3018    2705    1589    1146     310     208     146
 
EcoICRI GAG'CTC

Cuts at:   4546    6134    4546 
   Size:       1588    7534
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1838    2372    2671    2704    3077    3089    3587    3833    3836 
   Size:        534     299      33     373      12     498     246       3

Cuts at:   3836    3848    3986    4169    4390    4495    4669    4748    5096 
   Size:         12     138     183     221     105     174      79     348

Cuts at:   5096    5353    6947    7379    8126    8394    1838 
   Size:        257    1594     432     747     268    2566

  Fragments arranged by size: 

                2566    1594     747     534     498     432     373     348
                 299     268     257     246     221     183     174     138
                 105      79      33      12      12       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2887    3428    4219    4412    5068    2887 
   Size:        541     791     193     656    6941

  Fragments arranged by size: 

                6941     791     656     541     193
 
EcoO109I rG'GnC_Cy

Cuts at:    254     708     715     814    1072    1384    1401    2219    2275 
   Size:        454       7      99     258     312      17     818      56

Cuts at:   2275    2389    2390    2502    2587    2696    3284    3349    3368 
   Size:        114       1     112      85     109     588      65      19

Cuts at:   3368    3369    3472    3567    3607    3608    3662    3975    3988 
   Size:          1     103      95      40       1      54     313      13

Cuts at:   3988    3989    4139    4173    4317    4696    4916    4976    5621 
   Size:          1     150      34     144     379     220      60     645

Cuts at:   5621    5713    5783    6261     254 
   Size:         92      70     478    3115

  Fragments arranged by size: 

                3115     818     645     588     478     454     379     313
                 312     258     220     150     144     114     112     109
                 103      99      95      92      85      70      65      60
                  56      54      40      34      19      17      13       7
                   1       1       1       1
 
EcoRI G'AATT_C

Cuts at:   6126    6126 
   Size:       9122
 
EcoRV GAT'ATC

Cuts at:   1761    1761 
   Size:       9122
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    373    3450    7141     373 
   Size:       3077    3691    2354

  Fragments arranged by size: 

                3691    3077    2354
 
FspI TGC'GCA

Cuts at:   6903    6903 
   Size:       9122
 
HaeII r_GCGC'y

Cuts at:   4789    5515    6556    6804    7826    8805    4789 
   Size:        726    1041     248    1022     979    5106

  Fragments arranged by size: 

                5106    1041    1022     979     726     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2289    2321    2721    2753    5921    5953    5997    6028    6029 
   Size:         32     400      32    3168      32      44      31       1

Cuts at:   6029    6060    6964    6994    6996    7026    2289 
   Size:         31     904      30       2      30    4385

  Fragments arranged by size: 

                4385    3168     904     400      44      32      32      32
                  31      31      30      30       2       1
 
HincII GTy'rAC

Cuts at:    693    2832    4528    6161     693 
   Size:       2139    1696    1633    3654

  Fragments arranged by size: 

                3654    2139    1696    1633
 
KasI G'GCGC_C

Cuts at:   6552    6800    6552 
   Size:        248    8874
 
KpnI G_GTAC'C

Cuts at:   2450    6142    2450 
   Size:       3692    5430
 
MluI A'CGCG_T

Cuts at:   8555    8555 
   Size:       9122
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1887    2134    6020    7793    7977    1887 
   Size:        247    3886    1773     184    3032

  Fragments arranged by size: 

                3886    3032    1773     247     184
 
MscI TGG'CCA

Cuts at:    625    3187    6883     625 
   Size:       2562    3696    2864

  Fragments arranged by size: 

                3696    2864    2562
 
MslI CAynn'nnrTG

Cuts at:    677    1316    2139    2941    3044    4409    7111    7238     677 
   Size:        639     823     802     103    1365    2702     127    2561

  Fragments arranged by size: 

                2702    2561    1365     823     802     639     127     103
            
 
MspA1I CmG'CkG

Cuts at:    344     503     858    1916    2534    2594    2627    2837    3542 
   Size:        159     355    1058     618      60      33     210     705

Cuts at:   3542    4041    4280    4307    5053    6233    6547    6907    7920 
   Size:        499     239      27     746    1180     314     360    1013

Cuts at:   7920    8165     344 
   Size:        245    1301

  Fragments arranged by size: 

                1301    1180    1058    1013     746     705     618     499
                 360     355     314     245     239     210     159      60
                  33      27
 
NaeI GCC'GGC

Cuts at:   4609    5516    7303    4609 
   Size:        907    1787    6428

  Fragments arranged by size: 

                6428    1787     907
 
NarI GG'CG_CC

Cuts at:   6553    6801    6553 
   Size:        248    8874
 
NcoI C'CATG_G

Cuts at:   1311    2211    2489    3045    1311 
   Size:        900     278     556    7388

  Fragments arranged by size: 

                7388     900     556     278
 
NgoMIV G'CCGG_C

Cuts at:   4607    5514    7301    4607 
   Size:        907    1787    6428

  Fragments arranged by size: 

                6428    1787     907
 
NheI G'CTAG_C

Cuts at:   5508    5508 
   Size:       9122
 
NspI r_CATG'y

Cuts at:    546    1293    5868    6175    6675    7582     546 
   Size:        747    4575     307     500     907    2086

  Fragments arranged by size: 

                4575    2086     907     747     500     307
 
PasI CC'CwG_GG

Cuts at:    750    1068    3312    4215    4313    4321    4952    5973     750 
   Size:        318    2244     903      98       8     631    1021    3899

  Fragments arranged by size: 

                3899    2244    1021     903     631     318      98       8
            
 
PciI A'CATG_T

Cuts at:   1289    7578    1289 
   Size:       6289    2833
 
PflMI CCAn_nnn'nTGG

Cuts at:   1404    3183    1404 
   Size:       1779    7343
 
PfoI T'CCnGG_A

Cuts at:    704    1929    3779    4912     704 
   Size:       1225    1850    1133    4914

  Fragments arranged by size: 

                4914    1850    1225    1133
 
PmeI GTTT'AAAC

Cuts at:   6121    6121 
   Size:       9122
 
PmlI CAC'GTG

Cuts at:   2898    4751    2898 
   Size:       1853    7269
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3042    3074    8261    8293    3042 
   Size:         32    5187      32    3871

  Fragments arranged by size: 

                5187    3871      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    254     708     715     814    1072    1401    2219    2275    2587 
   Size:        454       7      99     258     329     818      56     312

Cuts at:   2587    3284    3472    3975    4173    4317    4916     254 
   Size:        697     188     503     198     144     599    4460

  Fragments arranged by size: 

                4460     818     697     599     503     454     329     312
                 258     198     188     144      99      56       7
 
PshAI GACnn'nnGTC

Cuts at:    470    1292    1412    1898    3134    6062     470 
   Size:        822     120     486    1236    2928    3530

  Fragments arranged by size: 

                3530    2928    1236     822     486     120
 
PspOMI G'GGCC_C

Cuts at:   1384    2389    2502    2695    3368    3567    3607    3988    4139 
   Size:       1005     113     193     673     199      40     381     151

Cuts at:   4139    5620    1384 
   Size:       1481    4886

  Fragments arranged by size: 

                4886    1481    1005     673     381     199     193     151
                 113      40
 
PstI C_TGCA'G

Cuts at:   1087    3171    3361    3682    3933    3961    4359    5187    5741 
   Size:       2084     190     321     251      28     398     828     554

Cuts at:   5741    5862    6169    6854    1087 
   Size:        121     307     685    3355

  Fragments arranged by size: 

                3355    2084     828     685     554     398     321     307
                 251     190     121      28
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9122
 
PvuII CAG'CTG

Cuts at:    344     858    1916    2534    2627    3542    4041    6547    6907 
   Size:        514    1058     618      93     915     499    2506     360

Cuts at:   6907     344 
   Size:       2559

  Fragments arranged by size: 

                2559    2506    1058     915     618     514     499     360
                  93
 
RsrII CG'GwC_CG

Cuts at:   7317    7317 
   Size:       9122
 
SacI G_AGCT'C

Cuts at:   4548    6136    4548 
   Size:       1588    7534
 
SalI G'TCGA_C

Cuts at:   2830    4526    6159    2830 
   Size:       1696    1633    5793

  Fragments arranged by size: 

                5793    1696    1633
 
SanDI GG'GwC_CC

Cuts at:   1072    2587    3975    4173    4317    1072 
   Size:       1515    1388     198     144    5877

  Fragments arranged by size: 

                5877    1515    1388     198     144
 
SapI GCTCTTCn'nnn_

Cuts at:   4505    7145    7355    4505 
   Size:       2640     210    6272

  Fragments arranged by size: 

                6272    2640     210
 
SbfI CC_TGCA'GG

Cuts at:   6169    6169 
   Size:       9122
 
ScaI AGT'ACT

Cuts at:   3880    8639    3880 
   Size:       4759    4363
 
SexAI A'CCwGG_T

Cuts at:   5252    5252 
   Size:       9122
 
SfcI C'TryA_G

Cuts at:     33    1083    2261    2283    2550    3167    3357    3678    3929 
   Size:       1050    1178      22     267     617     190     321     251

Cuts at:   3929    3957    4355    5183    5737    5858    6165    6850    7843 
   Size:         28     398     828     554     121     307     685     993

Cuts at:   7843    8034      33 
   Size:        191    1121

  Fragments arranged by size: 

                1178    1121    1050     993     828     685     617     554
                 398     321     307     267     251     191     190     121
                  28      22
 
SfoI GGC'GCC

Cuts at:   6554    6802    6554 
   Size:        248    8874
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9122
 
SgrAI Cr'CCGG_yG

Cuts at:   5514    5514 
   Size:       9122
 
SmaI CCC'GGG

Cuts at:   1205    3689    4057    4144    6144    1205 
   Size:       2484     368      87    2000    4183

  Fragments arranged by size: 

                4183    2484    2000     368      87
 
SmlI C'TyrA_G

Cuts at:   4639    5068    6277    7192    7684    7946    4639 
   Size:        429    1209     915     492     262    5815

  Fragments arranged by size: 

                5815    1209     915     492     429     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       9122
 
SphI G_CATG'C

Cuts at:    546    5868    6175    6675     546 
   Size:       5322     307     500    2993

  Fragments arranged by size: 

                5322    2993     500     307
 
SrfI GCCC'GGGC

Cuts at:   4144    4144 
   Size:       9122
 
StuI AGG'CCT

Cuts at:    517    2463    2822    2919    3407    3963     517 
   Size:       1946     359      97     488     556    5676

  Fragments arranged by size: 

                5676    1946     556     488     359      97
 
StyI C'CwwG_G

Cuts at:    369     826    1311    2211    2489    2784    2874    3045    3335 
   Size:        457     485     900     278     295      90     171     290

Cuts at:   3335    3632    4491    4923    6096    6256    6610     369 
   Size:        297     859     432    1173     160     354    2881

  Fragments arranged by size: 

                2881    1173     900     859     485     457     432     354
                 297     295     290     278     171     160      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    271     417    1577    3812    4243    4252    5955    6705    6838 
   Size:        146    1160    2235     431       9    1703     750     133

Cuts at:   6838    7297    7506     271 
   Size:        459     209    1887

  Fragments arranged by size: 

                2235    1887    1703    1160     750     459     431     209
                 146     133       9
 
TatI w'GTAC_w

Cuts at:   3055    3878    4905    8637    3055 
   Size:        823    1027    3732    3540

  Fragments arranged by size: 

                3732    3540    1027     823
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1276    1308    2358    2390    3775    3807    3967    3999    4089 
   Size:         32    1050      32    1385      32     160      32      90

Cuts at:   4089    4121    4285    4317    4700    4732    8639    8671    1276 
   Size:         32     164      32     383      32    3907      32    1727

  Fragments arranged by size: 

                3907    1727    1385    1050     383     164     160      90
                  32      32      32      32      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   4587    5643    5697    5751    6919    4587 
   Size:       1056      54      54    1168    6790

  Fragments arranged by size: 

                6790    1168    1056      54      54
 
XbaI T'CTAG_A

Cuts at:   6153    6153 
   Size:       9122
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    268     964    1111    1309    1813    2863    3211    5241    6617 
   Size:        696     147     198     504    1050     348    2030    1376

Cuts at:   6617     268 
   Size:       2773

  Fragments arranged by size: 

                2773    2030    1376    1050     696     504     348     198
                 147
 
XmaI C'CCGG_G

Cuts at:   1203    3687    4055    4142    6142    1203 
   Size:       2484     368      87    2000    4183

  Fragments arranged by size: 

                4183    2484    2000     368      87
 
ZraI GAC'GTC

Cuts at:   2796    5523    2796 
   Size:       2727    6395
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AleI     AscI    BfrBI    BsiWI     BsmI    BsrGI
   BstEII  BstZ17I     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NotI     NruI     NsiI     PacI     PsiI    PspXI     PsrI    SacII
     SfiI    SnaBI     SspI     SwaI     XhoI     XmnI
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