Restriction map for pF1KE2326
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BbrPI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsgI        BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DsaI        EarI        
Eco47III    Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        KpnI        
Ksp632I     KspI        MfeI        MluI        MseI        
MspA1I      MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        1315  GACGTC               GACGT^C
AhdI         1315  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1315  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI           54  GGCGCC               GGCGC^C
BbvCI         288  CCTCAGC              CCTCAGC(-5/-2)
BcgI          389  GCAACTATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          322  CTAG                 C^TAG
BglI          901  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         151  TACGTG               [CT]AC^GT[GA]
BsaBI        1341  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI          41  GAGACG               CGTCTC(1/5)
BsmFI         305  GGGAC                GGGAC(10/14)
BsrDI        1061  CATTGC               GCAATG(2/0)
BsrI         1560  CCAGT                ACTGG(1/-1)
BstAPI       1056  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1510  TTCGAA               TT^CGAA
BstEII        969  GGTCACC              G^GT[ACGT]ACC
BstPI         969  GGTCACC              G^GT[ACGT]ACC
BstXI        1154  CCAACATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         183  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI          943  GACAAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1315  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      183  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       969  GGTCACC              G^GT[ACGT]ACC
HgaI          457  GACGC                GACGC(5/10)
HincII        406  GTTGAC               GT[CT]^[GA]AC
HindII        406  GTTGAC               GT[CT]^[GA]AC
HindIII      1017  AAGCTT               A^AGCTT
KasI           54  GGCGCC               G^GCGCC
MaeI          322  CTAG                 C^TAG
MamI         1341  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI           54  GGCGCC               GG^CGCC
NspV         1510  TTCGAA               TT^CGAA
PleI          409  GACTC                GAGTC(4/5)
SfoI           54  GGCGCC               GGC^GCC
SfuI         1510  TTCGAA               TT^CGAA
SmaI         1208  CCCGGG               CCC^GGG
StuI          899  AGGCCT               AGG^CCT
XmaCI        1208  CCCGGG               C^CCGGG
XmaI         1208  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          38  CGCG                 CG^CG
              403  CGCG                
AccIII        599  TCCGGA               T^CCGGA
             1491  TCCGGA              
AcyI           54  GGCGCC               G[GA]^CG[CT]C
             1315  GACGTC              
AspHI        1403  GAGCTC               G[AT]GC[AT]^C
             1453  GAGCTC              
BanI           54  GGCGCC               G^G[CT][GA]CC
              714  GGTGCC              
BciVI        1417  GGATAC               GTATCC(6/5)
             1331  GTATCC              
BsaHI          54  GGCGCC               G[GA]^CG[CT]C
             1315  GACGTC              
BseAI         599  TCCGGA               T^CCGGA
             1491  TCCGGA              
BsiEI        1359  CGGCCG               CG[GA][CT]^CG
             1501  CGGCCG              
BsiHKAI      1403  GAGCTC               G[AT]GC[AT]^C
             1453  GAGCTC              
BspEI         599  TCCGGA               T^CCGGA
             1491  TCCGGA              
BspHI         503  TCATGA               T^CATGA
             1466  TCATGA              
BsrBI          49  GAGCGG               CCGCTC(-3/-3)
             1498  GAGCGG              
BstUI          38  CGCG                 CG^CG
              403  CGCG                
EagI         1359  CGGCCG               C^GGCCG
             1501  CGGCCG              
EclXI        1359  CGGCCG               C^GGCCG
             1501  CGGCCG              
Eco52I       1359  CGGCCG               C^GGCCG
             1501  CGGCCG              
EcoNI         906  CCTGGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1369  CCTTACCCAGG         
Hin1I          54  GGCGCC               G[GA]^CG[CT]C
             1315  GACGTC              
MaeIII        970  GTCAC                ^GT[ACGT]AC
             1351  GTCAC               
MroI          599  TCCGGA               T^CCGGA
             1491  TCCGGA              
MvnI           38  CGCG                 CG^CG
              403  CGCG                
PstI          655  CTGCAG               CTGCA^G
              934  CTGCAG              
RcaI          503  TCATGA               T^CATGA
             1466  TCATGA              
SacI         1403  GAGCTC               GAGCT^C
             1453  GAGCTC              
SfcI          655  CTGCAG               C^T[GA][CT]AG
              934  CTGCAG              
SmlI           12  CTCAAG               C^T[CT][GA]AG
              784  CTCAAG              
Tsp45I        970  GTCAC                ^GT[GC]AC
             1351  GTCAC               
TspRI         139  CACTG                CA[GC]TG[ACGT][ACGT]^
              863  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaI        AvaII       BalI        BamHI       BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsiYI       BslI        BsmAI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EaeI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TfiI        TseI        
TthHB8I     Van91I      XhoII       
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