Restriction map for pF1KE2326
 (Linear) (Six-base) MAPSORT of: KIEE2326.seq  Check: 3319  from: 1  to: 1578



KIEE2326.orf

 With 182 enzymes: SgfI * 

                        February 11, 2011 17:15  ..

AatII G_ACGT'C

Cuts at:      0    1319    1578 
   Size:       1319     259
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     630    1298    1412    1578 
   Size:        630     668     114     166

  Fragments arranged by size: 

                 668     630     166     114
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1320    1578 
   Size:       1320     258
 
AleI CACnn'nnGTG

Cuts at:      0     856    1578 
   Size:        856     722
 
AlwNI CAG_nnn'CTG

Cuts at:      0     232     663     708    1276    1578 
   Size:        232     431      45     568     302

  Fragments arranged by size: 

                 568     431     302     232      45
 
AvaI C'yCGr_G

Cuts at:      0     773    1208    1238    1578 
   Size:        773     435      30     340

  Fragments arranged by size: 

                 773     435     340      30
 
BamHI G'GATC_C

Cuts at:      0      24     159    1364    1578 
   Size:         24     135    1205     214

  Fragments arranged by size: 

                1205     214     135      24
 
BanI G'GyrC_C

Cuts at:      0      54     714    1578 
   Size:         54     660     864

  Fragments arranged by size: 

                 864     660      54
 
BanII G_rGCy'C

Cuts at:      0     499     698     983    1407    1457    1578 
   Size:        499     199     285     424      50     121

  Fragments arranged by size: 

                 499     424     285     199     121      50
 
BbeI G_GCGC'C

Cuts at:      0      58    1578 
   Size:         58    1520
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     290     719     874    1578 
   Size:        290     429     155     704

  Fragments arranged by size: 

                 704     429     290     155
 
BbvCI CC'TCA_GC

Cuts at:      0     289    1578 
   Size:        289    1289
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     378     412    1578 
   Size:        378      34    1166

  Fragments arranged by size: 

                1166     378      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1342    1411    1578 
   Size:       1342      69     167

  Fragments arranged by size: 

                1342     167      69
 
BglI GCCn_nnn'nGGC

Cuts at:      0     907    1578 
   Size:        907     671
 
Bme1580I G_kGCm'C

Cuts at:      0     719    1578 
   Size:        719     859
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     246    1578 
   Size:        246    1332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     436     811    1429    1578 
   Size:         73     363     375     618     149

  Fragments arranged by size: 

                 618     375     363     149      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     289    1578 
   Size:        289    1289
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1578 
   Size:        769     809
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     514    1133    1170    1578 
   Size:        514     619      37     408

  Fragments arranged by size: 

                 619     514     408      37
 
BsaAI yAC'GTr

Cuts at:      0     153    1578 
   Size:        153    1425
 
BsaBI GATnn'nnATC

Cuts at:      0    1345    1578 
   Size:       1345     233
 
BsaHI Gr'CG_yC

Cuts at:      0      55    1316    1578 
   Size:         55    1261     262

  Fragments arranged by size: 

                1261     262      55
 
BsaWI w'CCGG_w

Cuts at:      0     599    1421    1491    1578 
   Size:        599     822      70      87

  Fragments arranged by size: 

                 822     599      87      70
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     566     596    1279    1309    1578 
   Size:        566      30     683      30     269

  Fragments arranged by size: 

                 683     566     269      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     439    1465    1510    1578 
   Size:         61     378    1026      45      68

  Fragments arranged by size: 

                1026     378      68      61      45
 
BseYI C'CCAG_C

Cuts at:      0     492     808    1578 
   Size:        492     316     770

  Fragments arranged by size: 

                 770     492     316
 
BsiEI CG_ry'CG

Cuts at:      0    1362    1504    1578 
   Size:       1362     142      74

  Fragments arranged by size: 

                1362     142      74
 
BsiHKAI G_wGCw'C

Cuts at:      0    1407    1457    1578 
   Size:       1407      50     121

  Fragments arranged by size: 

                1407     121      50
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      35    1578 
   Size:         35    1543
 
Bsp1286I G_dGCh'C

Cuts at:      0     499     698     719     983    1407    1457    1578 
   Size:        499     199      21     264     424      50     121

  Fragments arranged by size: 

                 499     424     264     199     121      50      21
 
BspEI T'CCGG_A

Cuts at:      0     599    1491    1578 
   Size:        599     892      87

  Fragments arranged by size: 

                 892     599      87
 
BspHI T'CATG_A

Cuts at:      0     503    1466    1578 
   Size:        503     963     112

  Fragments arranged by size: 

                 963     503     112
 
BsrBI CCG'CTC

Cuts at:      0      51    1500    1578 
   Size:         51    1449      78

  Fragments arranged by size: 

                1449      78      51
 
BsrDI GCAATG_nn'

Cuts at:      0    1060    1578 
   Size:       1060     518
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1062    1578 
   Size:       1062     516
 
BstBI TT'CG_AA

Cuts at:      0    1511    1578 
   Size:       1511      67
 
BstEII G'GTnAC_C

Cuts at:      0     969    1578 
   Size:        969     609
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1161    1578 
   Size:       1161     417
 
BstYI r'GATC_y

Cuts at:      0      24     159     596     767    1364    1578 
   Size:         24     135     437     171     597     214

  Fragments arranged by size: 

                 597     437     214     171     135      24
 
BtsI GCAGTG_nn'

Cuts at:      0     869    1578 
   Size:        869     709
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     949    1578 
   Size:        949     629
 
EaeI y'GGCC_r

Cuts at:      0     359     833    1007    1359    1501    1547    1578 
   Size:        359     474     174     352     142      46      31

  Fragments arranged by size: 

                 474     359     352     174     142      46      31
 
EagI C'GGCC_G

Cuts at:      0    1359    1501    1578 
   Size:       1359     142      77

  Fragments arranged by size: 

                1359     142      77
 
EcoICRI GAG'CTC

Cuts at:      0    1405    1455    1578 
   Size:       1405      50     123

  Fragments arranged by size: 

                1405     123      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     436     630     811    1298    1412    1429    1578 
   Size:         73     363     194     181     487     114      17     149

  Fragments arranged by size: 

                 487     363     194     181     149     114      73      17
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     910    1373    1578 
   Size:        910     463     205

  Fragments arranged by size: 

                 910     463     205
 
EcoO109I rG'GnC_Cy

Cuts at:      0     184    1578 
   Size:        184    1394
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     557    1578 
   Size:        557    1021
 
HaeII r_GCGC'y

Cuts at:      0      12      58     131     468    1578 
   Size:         12      46      73     337    1110

  Fragments arranged by size: 

                1110     337      73      46      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     915     947    1306    1338    1389    1421 
   Size:        151      32     732      32     359      32      51      32

Cuts at:   1421    1578 
   Size:        157

  Fragments arranged by size: 

                 732     359     157     151      51      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     408    1578 
   Size:        408    1170
 
HindIII A'AGCT_T

Cuts at:      0    1017    1578 
   Size:       1017     561
 
KasI G'GCGC_C

Cuts at:      0      54    1578 
   Size:         54    1524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      75    1578 
   Size:         75    1503
 
MscI TGG'CCA

Cuts at:      0     361     835    1009    1578 
   Size:        361     474     174     569

  Fragments arranged by size: 

                 569     474     361     174
 
MslI CAynn'nnrTG

Cuts at:      0     244     856     892    1468    1578 
   Size:        244     612      36     576     110

  Fragments arranged by size: 

                 612     576     244     110      36
 
NarI GG'CG_CC

Cuts at:      0      55    1578 
   Size:         55    1523
 
NspI r_CATG'y

Cuts at:      0     492     726     891    1578 
   Size:        492     234     165     687

  Fragments arranged by size: 

                 687     492     234     165
 
PasI CC'CwG_GG

Cuts at:      0    1375    1578 
   Size:       1375     203
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315     474    1265    1578 
   Size:        315     159     791     313

  Fragments arranged by size: 

                 791     315     313     159
 
PfoI T'CCnGG_A

Cuts at:      0     509    1578 
   Size:        509    1069
 
PstI C_TGCA'G

Cuts at:      0     659     938    1578 
   Size:        659     279     640

  Fragments arranged by size: 

                 659     640     279
 
SacI G_AGCT'C

Cuts at:      0    1407    1457    1578 
   Size:       1407      50     121

  Fragments arranged by size: 

                1407     121      50
 
SfcI C'TryA_G

Cuts at:      0     655     934    1578 
   Size:        655     279     644

  Fragments arranged by size: 

                 655     644     279
 
SfoI GGC'GCC

Cuts at:      0      56    1578 
   Size:         56    1522
 
SmaI CCC'GGG

Cuts at:      0    1210    1578 
   Size:       1210     368
 
SmlI C'TyrA_G

Cuts at:      0      12     784    1578 
   Size:         12     772     794

  Fragments arranged by size: 

                 794     772      12
 
StuI AGG'CCT

Cuts at:      0     901    1578 
   Size:        901     677
 
StyI C'CwwG_G

Cuts at:      0     745     854     974    1578 
   Size:        745     109     120     604

  Fragments arranged by size: 

                 745     604     120     109
 
TatI w'GTAC_w

Cuts at:      0    1229    1578 
   Size:       1229     349
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     566     598    1578 
   Size:        566      32     980

  Fragments arranged by size: 

                 980     566      32
 
XmaI C'CCGG_G

Cuts at:      0    1208    1578 
   Size:       1208     370
 
ZraI GAC'GTC

Cuts at:      0    1317    1578 
   Size:       1317     261
 

 Enzymes that do cut:

    AatII     AcuI     AhdI     AleI    AlwNI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtsI     DrdI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI
     MscI     MslI     NarI     NspI     PasI    PflMI     PfoI     PstI
     SacI     SfcI     SfoI     SmaI     SmlI     StuI     StyI     TatI
     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BsgI    BsiWI     BsmI    BspMI    BsrFI    BsrGI   BssHII
    BssSI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     EarI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     KpnI     MfeI     MluI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmnI
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