Restriction map for pF1KE2727
##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       AccI        AclI        AflII       Alw44I      
Aor51HI     ApaLI       AviII       AvrII       BbrPI       
BcgI        BclI        BfrI        BlnI        BsaAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstZ17I     
ClaI        DraI        DraIII      Eco47III    EcoRV       
EcoT22I     FbaI        FspI        HpaI        KspI        
MluI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
SacII       SalI        SapI        ScaI        SfiI        
SfuI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        XbaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        581  TCCGGA               T^CCGGA
AflIII       2786  ACGTGT               A^C[GA][CT]GT
AgeI          632  ACCGGT               A^CCGGT
AhdI         1432  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2853  CCTGAGG              CC^T[ACGT]AGG
AscI         2329  GGCGCGCC             GG^CGCGCC
AseI         2188  ATTAAT               AT^TAAT
AsnI         2188  ATTAAT               AT^TAAT
AspEI        1432  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          655  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        2882  GTATCC               GTATCC(6/5)
BlpI          880  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      880  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2757  GATCGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         581  TCCGGA               T^CCGGA
BsmBI        3033  CGTCTC               CGTCTC(1/5)
BspEI         581  TCCGGA               T^CCGGA
BstAPI        402  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2853  CCTGAGG              CC^T[ACGT]AGG
CelII         880  GCTCAGC              GC^T[ACGT]AGC
CpoI          121  CGGACCG              CG^G[AT]CCG
Eam1105I     1432  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2853  CCTGAGG              CC^T[ACGT]AGG
FseI          246  GGCCGGCC             GGCCGG^CC
HindIII      1277  AAGCTT               A^AGCTT
MamI         2757  GATCGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3271  CAATTG               C^AATTG
MroI          581  TCCGGA               T^CCGGA
MseI         2189  TTAA                 T^TAA
MunI         3271  CAATTG               C^AATTG
PaeR7I       3316  CTCGAG               C^TCGAG
PflFI         655  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         632  ACCGGT               A^CCGGT
PshBI        2188  ATTAAT               AT^TAAT
RsrII         121  CGGACCG              CG^G[AT]CCG
SbfI         1038  CCTGCAGG             CCTGCA^GG
SexAI         347  ACCTGGT              A^CC[AT]GGT
Sse8387I     1038  CCTGCAGG             CCTGCA^GG
Tru9I        2189  TTAA                 T^TAA
Tth111I       655  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3316  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Acc65I        629  GGTACC               G^GTACC
             1553  GGTACC              
AcsI         1114  GAATTC               [GA]^AATT[CT]
             3202  GAATTC              
ApoI         1114  GAATTC               [GA]^AATT[CT]
             3202  GAATTC              
Asp700I       738  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1234  GAACTCTTTC          
Asp718I       629  GGTACC               G^GTACC
             1553  GGTACC              
BamHI          29  GGATCC               G^GATCC
              291  GGATCC              
BglII          38  AGATCT               A^GATCT
             2918  AGATCT              
BsaI          937  GAGACC               GGTCTC(1/5)
             1120  GAGACC              
BsaWI         581  TCCGGA               [AT]^CCGG[AT]
              632  ACCGGT              
BsiEI         249  CGGCCG               CG[GA][CT]^CG
              486  CGGCCG              
BsrDI        1221  CATTGC               GCAATG(2/0)
             3117  CATTGC              
BssHII       1544  GCGCGC               G^CGCGC
             2330  GCGCGC              
DrdI          808  GACATCCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1027  GACCTTGAGGTC        
EagI          249  CGGCCG               C^GGCCG
              486  CGGCCG              
EarI         2800  CTCTTC               CTCTTC(1/4)
             2140  GAAGAG              
EclXI         249  CGGCCG               C^GGCCG
              486  CGGCCG              
Eco52I        249  CGGCCG               C^GGCCG
              486  CGGCCG              
EcoRI        1114  GAATTC               G^AATTC
             3202  GAATTC              
KpnI          629  GGTACC               GGTAC^C
             1553  GGTACC              
Ksp632I      2800  CTCTTC               CTCTTC(1/4)
             2140  GAAGAG              
NcoI          143  CCATGG               C^CATGG
              554  CCATGG              
SgrAI        2296  CGCCGGTG             C[GA]^CCGG[CT]G
             2333  CGCCGGTG            
SmaI         2533  CCCGGG               CCC^GGG
             2652  CCCGGG              
SmlI         1030  CTTGAG               C^T[CT][GA]AG
             3316  CTCGAG              
StuI         1148  AGGCCT               AGG^CCT
             1166  AGGCCT              
XmaCI        2533  CCCGGG               C^CCGGG
             2652  CCCGGG              
XmaI         2533  CCCGGG               C^CCGGG
             2652  CCCGGG              
XmnI          738  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1234  GAACTCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       
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