Restriction map for pF1KE2727
 (Linear) (Six-base) MAPSORT of: KIEE2727.seq  Check: 6955  from: 1  to: 3642



KIEE2727.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2626    3037    3642 
   Size:       2626     411     605

  Fragments arranged by size: 

                2626     605     411
 
Acc65I G'GTAC_C

Cuts at:      0     629    1553    3642 
   Size:        629     924    2089

  Fragments arranged by size: 

                2089     924     629
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2112    3463    3642 
   Size:       2112    1351     179

  Fragments arranged by size: 

                2112    1351     179
 
AflIII A'CryG_T

Cuts at:      0    2786    3642 
   Size:       2786     856
 
AgeI A'CCGG_T

Cuts at:      0     632    3642 
   Size:        632    3010
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1437    3642 
   Size:       1437    2205
 
AleI CACnn'nnGTG

Cuts at:      0     544    2034    3642 
   Size:        544    1490    1608

  Fragments arranged by size: 

                1608    1490     544
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1630    3642 
   Size:       1630    2012
 
AlwNI CAG_nnn'CTG

Cuts at:      0     408    2022    2496    2565    2975    3513    3642 
   Size:        408    1614     474      69     410     538     129

  Fragments arranged by size: 

                1614     538     474     410     408     129      69
 
ApaI G_GGCC'C

Cuts at:      0     382    2076    2533    2753    3018    3642 
   Size:        382    1694     457     220     265     624

  Fragments arranged by size: 

                1694     624     457     382     265     220
 
ApoI r'AATT_y

Cuts at:      0    1114    3202    3642 
   Size:       1114    2088     440

  Fragments arranged by size: 

                2088    1114     440
 
AscI GG'CGCG_CC

Cuts at:      0    2330    3642 
   Size:       2330    1312
 
AseI AT'TA_AT

Cuts at:      0    2189    3642 
   Size:       2189    1453
 
AvaI C'yCGr_G

Cuts at:      0     992    1660    2533    2652    3316    3642 
   Size:        992     668     873     119     664     326

  Fragments arranged by size: 

                 992     873     668     664     326     119
 
BamHI G'GATC_C

Cuts at:      0      29     291    3642 
   Size:         29     262    3351

  Fragments arranged by size: 

                3351     262      29
 
BanI G'GyrC_C

Cuts at:      0       6     113     538     629    1398    1453    1553    1567 
   Size:          6     107     425      91     769      55     100      14

Cuts at:   1567    2294    2300    2421    3025    3642 
   Size:        727       6     121     604     617

  Fragments arranged by size: 

                 769     727     617     604     425     121     107     100
                  91      55      14       6       6
 
BanII G_rGCy'C

Cuts at:      0     382     616    1079    1162    1493    2076    2150    2533 
   Size:        382     234     463      83     331     583      74     383

Cuts at:   2533    2753    2852    2990    3018    3295    3642 
   Size:        220      99     138      28     277     347

  Fragments arranged by size: 

                 583     463     383     382     347     331     277     234
                 220     138      99      83      74      28
 
BbeI G_GCGC'C

Cuts at:      0     117    1402    1571    2298    2425    3642 
   Size:        117    1285     169     727     127    1217

  Fragments arranged by size: 

                1285    1217     727     169     127     117
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      58     787     953    2236    2889    3642 
   Size:         58     729     166    1283     653     753

  Fragments arranged by size: 

                1283     753     729     653     166      58
 
BbvCI CC'TCA_GC

Cuts at:      0    1170    1345    1519    1761    1817    3642 
   Size:       1170     175     174     242      56    1825

  Fragments arranged by size: 

                1825    1170     242     175     174      56
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2893    3642 
   Size:       2893     749
 
BglI GCCn_nnn'nGGC

Cuts at:      0     160     731     877    2258    3642 
   Size:        160     571     146    1381    1384

  Fragments arranged by size: 

                1384    1381     571     160     146
 
BglII A'GATC_T

Cuts at:      0      38    2918    3642 
   Size:         38    2880     724

  Fragments arranged by size: 

                2880     724      38
 
BlpI GC'TnA_GC

Cuts at:      0     881    3642 
   Size:        881    2761
 
Bme1580I G_kGCm'C

Cuts at:      0     342     382     541    1698    1716    2076    2533    2753 
   Size:        342      40     159    1157      18     360     457     220

Cuts at:   2753    3003    3018    3028    3642 
   Size:        250      15      10     614

  Fragments arranged by size: 

                1157     614     457     360     342     250     220     159
                  40      18      15      10
 
BmgBI CAC'GTC

Cuts at:      0    1506    2787    2952    3452    3642 
   Size:       1506    1281     165     500     190

  Fragments arranged by size: 

                1506    1281     500     190     165
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     473     542    3033    3642 
   Size:        473      69    2491     609

  Fragments arranged by size: 

                2491     609     473      69
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     613    3447    3642 
   Size:        613    2834     195

  Fragments arranged by size: 

                2834     613     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     778    1695    1825    1948    2458    3544    3642 
   Size:        373     405     917     130     123     510    1086      98

  Fragments arranged by size: 

                1086     917     510     405     373     130     123      98
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     909    1170    1345    1519    1761    1817    2497    3265 
   Size:        909     261     175     174     242      56     680     768

Cuts at:   3265    3577    3642 
   Size:        312      65

  Fragments arranged by size: 

                 909     768     680     312     261     242     175     174
                  65      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    3642 
   Size:       1051    2591
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     931    1114    3642 
   Size:        931     183    2528

  Fragments arranged by size: 

                2528     931     183
 
BsaBI GATnn'nnATC

Cuts at:      0    2761    3642 
   Size:       2761     881
 
BsaHI Gr'CG_yC

Cuts at:      0     114    1292    1399    1438    1568    1985    2233    2295 
   Size:        114    1178     107      39     130     417     248      62

Cuts at:   2295    2422    3642 
   Size:        127    1220

  Fragments arranged by size: 

                1220    1178     417     248     130     127     114     107
                  62      39
 
BsaWI w'CCGG_w

Cuts at:      0     581     632    3642 
   Size:        581      51    3010

  Fragments arranged by size: 

                3010     581      51
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1594    1624    2152    2182    3642 
   Size:       1594      30     528      30    1460

  Fragments arranged by size: 

                1594    1460     528      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     814     886    1834    1912    1915    2158    2173    3058 
   Size:        814      72     948      78       3     243      15     885

Cuts at:   3058    3642 
   Size:        584

  Fragments arranged by size: 

                 948     885     814     584     243      78      72      15
                   3
 
BseYI C'CCAG_C

Cuts at:      0     363    1464    1530    2220    2238    2745    3296    3483 
   Size:        363    1101      66     690      18     507     551     187

Cuts at:   3483    3642 
   Size:        159

  Fragments arranged by size: 

                1101     690     551     507     363     187     159      66
                  18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     457    3297    3642 
   Size:         42     415    2840     345

  Fragments arranged by size: 

                2840     415     345      42
 
BsiEI CG_ry'CG

Cuts at:      0     252     489    3642 
   Size:        252     237    3153

  Fragments arranged by size: 

                3153     252     237
 
BsiHKAI G_wGCw'C

Cuts at:      0     445    1079    1162    1493    2969    3642 
   Size:        445     634      83     331    1476     673

  Fragments arranged by size: 

                1476     673     634     445     331      83
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3039    3642 
   Size:       3039     603
 
Bsp1286I G_dGCh'C

Cuts at:      0     342     382     445     541     616    1079    1162    1493 
   Size:        342      40      63      96      75     463      83     331

Cuts at:   1493    1698    1716    2076    2150    2533    2753    2852    2969 
   Size:        205      18     360      74     383     220      99     117

Cuts at:   2969    2990    3003    3018    3028    3295    3642 
   Size:         21      13      15      10     267     347

  Fragments arranged by size: 

                 463     383     360     347     342     331     267     220
                 205     117      99      96      83      75      74      63
                  40      21      18      15      13      10
 
BspEI T'CCGG_A

Cuts at:      0     581    3642 
   Size:        581    3061
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      64     416    1691    2378    2626    2921    3037    3632 
   Size:         64     352    1275     687     248     295     116     595

Cuts at:   3632    3642 
   Size:         10

  Fragments arranged by size: 

                1275     687     595     352     295     248     116      64
                  10
 
BsrBI CCG'CTC

Cuts at:      0    1641    1941    2501    3354    3528    3584    3642 
   Size:       1641     300     560     853     174      56      58

  Fragments arranged by size: 

                1641     853     560     300     174      58      56
 
BsrDI GCAATG_nn'

Cuts at:      0    1220    3116    3642 
   Size:       1220    1896     526

  Fragments arranged by size: 

                1896    1220     526
 
BsrFI r'CCGG_y

Cuts at:      0     247     488     632    1307    2297    2334    2418    2642 
   Size:        247     241     144     675     990      37      84     224

Cuts at:   2642    3642 
   Size:       1000

  Fragments arranged by size: 

                1000     990     675     247     241     224     144      84
                  37
 
BssHII G'CGCG_C

Cuts at:      0    1544    2330    3642 
   Size:       1544     786    1312

  Fragments arranged by size: 

                1544    1312     786
 
BssSI C'ACGA_G

Cuts at:      0     898    2404    3085    3642 
   Size:        898    1506     681     557

  Fragments arranged by size: 

                1506     898     681     557
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     408    3642 
   Size:        408    3234
 
BstEII G'GTnAC_C

Cuts at:      0     171     643    2337    3642 
   Size:        171     472    1694    1305

  Fragments arranged by size: 

                1694    1305     472     171
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     549     561    2349    3054    3642 
   Size:        549      12    1788     705     588

  Fragments arranged by size: 

                1788     705     588     549      12
 
BstYI r'GATC_y

Cuts at:      0      29      38      84     291    2918    3642 
   Size:         29       9      46     207    2627     724

  Fragments arranged by size: 

                2627     724     207      46      29       9
 
Bsu36I CC'TnA_GG

Cuts at:      0    2854    3642 
   Size:       2854     788
 
BtgI C'CryG_G

Cuts at:      0     143     167     200     554    1338    2840    2860    3642 
   Size:        143      24      33     354     784    1502      20     782

  Fragments arranged by size: 

                1502     784     782     354     143      33      24      20
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     203    3642 
   Size:        203    3439
 
BtsI GCAGTG_nn'

Cuts at:      0     143    1905    3547    3642 
   Size:        143    1762    1642      95

  Fragments arranged by size: 

                1762    1642     143      95
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     814    1033    3642 
   Size:        814     219    2609

  Fragments arranged by size: 

                2609     814     219
 
EaeI y'GGCC_r

Cuts at:      0     140     249     486     524     551    1721    1970    2051 
   Size:        140     109     237      38      27    1170     249      81

Cuts at:   2051    2541    2837    3089    3566    3642 
   Size:        490     296     252     477      76

  Fragments arranged by size: 

                1170     490     477     296     252     249     237     140
                 109      81      76      38      27
 
EagI C'GGCC_G

Cuts at:      0     249     486    3642 
   Size:        249     237    3156

  Fragments arranged by size: 

                3156     249     237
 
EarI CTCTTCn'nnn_

Cuts at:      0    2135    2806    3642 
   Size:       2135     671     836

  Fragments arranged by size: 

                2135     836     671
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     949    2230    3642 
   Size:        949    1281    1412

  Fragments arranged by size: 

                1412    1281     949
 
EcoICRI GAG'CTC

Cuts at:      0    1077    1160    1491    3642 
   Size:       1077      83     331    2151

  Fragments arranged by size: 

                2151    1077     331      83
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     778    1695    1825    1948    2112    2458    3463 
   Size:        373     405     917     130     123     164     346    1005

Cuts at:   3463    3544    3642 
   Size:         81      98

  Fragments arranged by size: 

                1005     917     405     373     346     164     130     123
                  98      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1144    1583    1663    3340    3642 
   Size:       1144     439      80    1677     302

  Fragments arranged by size: 

                1677    1144     439     302      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0      95     378     379     405    1026    1035    1137    1575 
   Size:         95     283       1      26     621       9     102     438

Cuts at:   1575    1668    1772    2073    2200    2244    2493    2529    2530 
   Size:         93     104     301     127      44     249      36       1

Cuts at:   2530    2749    3014    3573    3642 
   Size:        219     265     559      69

  Fragments arranged by size: 

                 621     559     438     301     283     265     249     219
                 127     104     102      95      93      69      44      36
                  26       9       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1114    3202    3642 
   Size:       1114    2088     440

  Fragments arranged by size: 

                2088    1114     440
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1045    3408    3483    3642 
   Size:       1045    2363      75     159

  Fragments arranged by size: 

                2363    1045     159      75
 
FseI GG_CCGG'CC

Cuts at:      0     251    3642 
   Size:        251    3391
 
HaeII r_GCGC'y

Cuts at:      0     117    1402    1571    2298    2425    2565    3513    3642 
   Size:        117    1285     169     727     127     140     948     129

  Fragments arranged by size: 

                1285     948     727     169     140     129     127     117
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     986    1018    1090    1122    1423    1455    1596    1628 
   Size:        986      32      72      32     301      32     141      32

Cuts at:   1628    3642 
   Size:       2014

  Fragments arranged by size: 

                2014     986     301     141      72      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0      75     854    1872    2736    3108    3642 
   Size:         75     779    1018     864     372     534

  Fragments arranged by size: 

                1018     864     779     534     372      75
 
HindIII A'AGCT_T

Cuts at:      0    1277    3642 
   Size:       1277    2365
 
KasI G'GCGC_C

Cuts at:      0     113    1398    1567    2294    2421    3642 
   Size:        113    1285     169     727     127    1221

  Fragments arranged by size: 

                1285    1221     727     169     127     113
 
KpnI G_GTAC'C

Cuts at:      0     633    1557    3642 
   Size:        633     924    2085

  Fragments arranged by size: 

                2085     924     633
 
MfeI C'AATT_G

Cuts at:      0    3271    3642 
   Size:       3271     371
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1430    2963    3642 
   Size:       1430    1533     679

  Fragments arranged by size: 

                1533    1430     679
 
MscI TGG'CCA

Cuts at:      0     142     526     553    1723    1972    2053    2543    2839 
   Size:        142     384      27    1170     249      81     490     296

Cuts at:   2839    3091    3642 
   Size:        252     551

  Fragments arranged by size: 

                1170     551     490     384     296     252     249     142
                  81      27
 
MslI CAynn'nnrTG

Cuts at:      0     324     497     544     559    2034    2347    2950    3121 
   Size:        324     173      47      15    1475     313     603     171

Cuts at:   3121    3642 
   Size:        521

  Fragments arranged by size: 

                1475     603     521     324     313     173     171      47
                  15
 
MspA1I CmG'CkG

Cuts at:      0     298    1968    2238    2285    2312    2525    2588    2745 
   Size:        298    1670     270      47      27     213      63     157

Cuts at:   2745    3483    3642 
   Size:        738     159

  Fragments arranged by size: 

                1670     738     298     270     213     159     157      63
                  47      27
 
NaeI GCC'GGC

Cuts at:      0     249     490    2420    2644    3642 
   Size:        249     241    1930     224     998

  Fragments arranged by size: 

                1930     998     249     241     224
 
NarI GG'CG_CC

Cuts at:      0     114    1399    1568    2295    2422    3642 
   Size:        114    1285     169     727     127    1220

  Fragments arranged by size: 

                1285    1220     727     169     127     114
 
NcoI C'CATG_G

Cuts at:      0     143     554    3642 
   Size:        143     411    3088

  Fragments arranged by size: 

                3088     411     143
 
NgoMIV G'CCGG_C

Cuts at:      0     247     488    2418    2642    3642 
   Size:        247     241    1930     224    1000

  Fragments arranged by size: 

                1930    1000     247     241     224
 
NspI r_CATG'y

Cuts at:      0     496    1516    1611    3642 
   Size:        496    1020      95    2031

  Fragments arranged by size: 

                2031    1020     496      95
 
PasI CC'CwG_GG

Cuts at:      0     569     961    3642 
   Size:        569     392    2681

  Fragments arranged by size: 

                2681     569     392
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     522     967    3462    3642 
   Size:        522     445    2495     180

  Fragments arranged by size: 

                2495     522     445     180
 
PfoI T'CCnGG_A

Cuts at:      0      87    3197    3642 
   Size:         87    3110     445

  Fragments arranged by size: 

                3110     445      87
 
PpuMI rG'GwC_Cy

Cuts at:      0      95     405    1026    1035    1575    2244    3642 
   Size:         95     310     621       9     540     669    1398

  Fragments arranged by size: 

                1398     669     621     540     310      95       9
 
PspOMI G'GGCC_C

Cuts at:      0     378    2072    2529    2749    3014    3642 
   Size:        378    1694     457     220     265     628

  Fragments arranged by size: 

                1694     628     457     378     265     220
 
PspXI vC'TCGA_Gb

Cuts at:      0    3316    3642 
   Size:       3316     326
 
PstI C_TGCA'G

Cuts at:      0     303     404    1043    2918    3642 
   Size:        303     101     639    1875     724

  Fragments arranged by size: 

                1875     724     639     303     101
 
PvuII CAG'CTG

Cuts at:      0     298    1968    2588    2745    3483    3642 
   Size:        298    1670     620     157     738     159

  Fragments arranged by size: 

                1670     738     620     298     159     157
 
RsrII CG'GwC_CG

Cuts at:      0     122    3642 
   Size:        122    3520
 
SacI G_AGCT'C

Cuts at:      0    1079    1162    1493    3642 
   Size:       1079      83     331    2149

  Fragments arranged by size: 

                2149    1079     331      83
 
SanDI GG'GwC_CC

Cuts at:      0    1575    2244    3642 
   Size:       1575     669    1398

  Fragments arranged by size: 

                1575    1398     669
 
SbfI CC_TGCA'GG

Cuts at:      0    1043    3642 
   Size:       1043    2599
 
SexAI A'CCwGG_T

Cuts at:      0     347    3642 
   Size:        347    3295
 
SfcI C'TryA_G

Cuts at:      0     299     400    1039    1389    2914    3642 
   Size:        299     101     639     350    1525     728

  Fragments arranged by size: 

                1525     728     639     350     299     101
 
SfoI GGC'GCC

Cuts at:      0     115    1400    1569    2296    2423    3642 
   Size:        115    1285     169     727     127    1219

  Fragments arranged by size: 

                1285    1219     727     169     127     115
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2297    2334    3642 
   Size:       2297      37    1308

  Fragments arranged by size: 

                2297    1308      37
 
SmaI CCC'GGG

Cuts at:      0    2535    2654    3642 
   Size:       2535     119     988

  Fragments arranged by size: 

                2535     988     119
 
SmlI C'TyrA_G

Cuts at:      0    1030    3316    3642 
   Size:       1030    2286     326

  Fragments arranged by size: 

                2286    1030     326
 
StuI AGG'CCT

Cuts at:      0    1150    1168    3642 
   Size:       1150      18    2474

  Fragments arranged by size: 

                2474    1150      18
 
StyI C'CwwG_G

Cuts at:      0     143     542     554     608    3642 
   Size:        143     399      12      54    3034

  Fragments arranged by size: 

                3034     399     143      54      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     299     547     889    1598    2079    3552    3642 
   Size:        299     248     342     709     481    1473      90

  Fragments arranged by size: 

                1473     709     481     342     299     248      90
 
TatI w'GTAC_w

Cuts at:      0    1727    3147    3642 
   Size:       1727    1420     495

  Fragments arranged by size: 

                1727    1420     495
 
Tth111I GACn'n_nGTC

Cuts at:      0     658    3642 
   Size:        658    2984
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2073    3054    3099    3642 
   Size:       2073     981      45     543

  Fragments arranged by size: 

                2073     981     543      45
 
XhoI C'TCGA_G

Cuts at:      0    3316    3642 
   Size:       3316     326
 
XmaI C'CCGG_G

Cuts at:      0    2533    2652    3642 
   Size:       2533     119     990

  Fragments arranged by size: 

                2533     990     119
 
XmnI GAAnn'nnTTC

Cuts at:      0     742    1238    3642 
   Size:        742     496    2404

  Fragments arranged by size: 

                2404     742     496
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AgeI     AhdI     AleI     AlfI
    AlwNI     ApaI     ApoI     AscI     AseI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII     SacI
    SanDI     SbfI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI
     StuI     StyI    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AloI    ApaLI    AsiSI
    AvrII     BaeI     BcgI     BclI    BfrBI     BmtI    BsaAI    BsiWI
     BsmI    BspHI    BsrGI    BstBI  BstZ17I     ClaI    CspCI     DraI
   DraIII    EcoRV     FspI    FspAI     HpaI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    SacII     SalI     SapI     ScaI
     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI     XbaI     ZraI
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