Restriction map for pF1KE3496
##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        AspI        AvrII       BbvCI       
BcgI        BlnI        BspDI       BssHII      BssSI       
BstBI       BstEII      BstPI       ClaI        CpoI        
DraI        Eco47III    EcoO65I     EcoT22I     FseI        
HpaI        KspI        MluI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PshBI       PvuI        
RsrII       SacII       SfuI        SgrAI       SnaBI       
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        1654  GACGTC               GACGT^C
Acc65I        557  GGTACC               G^GTACC
AclI         3492  AACGTT               AA^CGTT
AocI          505  CCTGAGG              CC^T[ACGT]AGG
ApaI         2783  GGGCCC               GGGCC^C
Asp718I       557  GGTACC               G^GTACC
BbrPI         286  CACGTG               CAC^GTG
BlpI         2826  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2826  GCTGAGC              GC^T[ACGT]AGC
BsaAI         286  CACGTG               [CT]AC^GT[GA]
BsiEI        4394  CGGCCG               CG[GA][CT]^CG
BsiWI        4062  CGTACG               C^GTACG
Bsp1407I     3920  TGTACA               T^GTACA
BsrGI        3920  TGTACA               T^GTACA
Bst1107I     5556  GTATAC               GTA^TAC
BstZ17I      5556  GTATAC               GTA^TAC
Bsu36I        505  CCTGAGG              CC^T[ACGT]AGG
CelII        2826  GCTGAGC              GC^T[ACGT]AGC
EagI         4394  CGGCCG               C^GGCCG
EclXI        4394  CGGCCG               C^GGCCG
Eco52I       4394  CGGCCG               C^GGCCG
Eco81I        505  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3979  GAATTC               G^AATTC
EcoRV         382  GATATC               GAT^ATC
HindIII       157  AAGCTT               A^AGCTT
KpnI          557  GGTACC               GGTAC^C
MfeI         1800  CAATTG               C^AATTG
MunI         1800  CAATTG               C^AATTG
NdeI          456  CATATG               CA^TATG
PaeR7I       3443  CTCGAG               C^TCGAG
PmaCI         286  CACGTG               CAC^GTG
PmlI          286  CACGTG               CAC^GTG
Psp1406I     3492  AACGTT               AA^CGTT
SacI         3376  GAGCTC               GAGCT^C
SapI         3117  GCTCTTC              GCTCTTC(1/4)
SfiI          247  GGCCACTTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI           34  GCATGC               GCATG^C
SspBI        3920  TGTACA               T^GTACA
SspI         5597  AATATT               AAT^ATT
StuI         3188  AGGCCT               AGG^CCT
XhoI         3443  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII        1532  CGCG                 CG^CG
             2628  CGCG                
AccIII         81  TCCGGA               T^CCGGA
             2314  TCCGGA              
AflII        2725  CTTAAG               C^TTAAG
             3370  CTTAAG              
AhdI          234  GACAGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4247  GACACGGAGTC         
AspEI         234  GACAGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4247  GACACGGAGTC         
BamHI         569  GGATCC               G^GATCC
             4001  GGATCC              
BbeI         1243  GGCGCC               GGCGC^C
             4585  GGCGCC              
BciVI        2572  GGATAC               GTATCC(6/5)
             1805  GTATCC              
BclI         1904  TGATCA               T^GATCA
             2591  TGATCA              
BfrI         2725  CTTAAG               C^TTAAG
             3370  CTTAAG              
BglII         668  AGATCT               A^GATCT
             4091  AGATCT              
BsaI         4359  GGTCTC               GGTCTC(1/5)
             4694  GGTCTC              
BseAI          81  TCCGGA               T^CCGGA
             2314  TCCGGA              
BspEI          81  TCCGGA               T^CCGGA
             2314  TCCGGA              
BstAPI       4400  GCACTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4570  GCAACCCCTGC         
BstUI        1532  CGCG                 CG^CG
             2628  CGCG                
DrdI         2545  GACACGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2920  GACAGCAAGGTC        
Eam1105I      234  GACAGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4247  GACACGGAGTC         
FbaI         1904  TGATCA               T^GATCA
             2591  TGATCA              
HaeII        1243  GGCGCC               [GA]GCGC^[CT]
             4585  GGCGCC              
KasI         1243  GGCGCC               G^GCGCC
             4585  GGCGCC              
MroI           81  TCCGGA               T^CCGGA
             2314  TCCGGA              
MvnI         1532  CGCG                 CG^CG
             2628  CGCG                
NarI         1243  GGCGCC               GG^CGCC
             4585  GGCGCC              
SalI         1701  GTCGAC               G^TCGAC
             3089  GTCGAC              
SbfI         3891  CCTGCAGG             CCTGCA^GG
             3990  CCTGCAGG            
SfoI         1243  GGCGCC               GGC^GCC
             4585  GGCGCC              
SmaI         3247  CCCGGG               CCC^GGG
             4713  CCCGGG              
SpeI         1212  ACTAGT               A^CTAGT
             5314  ACTAGT              
Sse8387I     3891  CCTGCAGG             CCTGCA^GG
             3990  CCTGCAGG            
XmaCI        3247  CCCGGG               C^CCGGG
             4713  CCCGGG              
XmaI         3247  CCCGGG               C^CCGGG
             4713  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       AviII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaBI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FspI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SexAI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmnI        
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