Restriction map for pF1KE3496
 (Linear) (Six-base) MAPSORT of: KIEE3496.seq  Check: 346  from: 1  to: 5910



KIEE3496.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1325    1520    3140    4800    5910 
   Size:       1325     195    1620    1660    1110

  Fragments arranged by size: 

                1660    1620    1325    1110     195
 
AatII G_ACGT'C

Cuts at:      0    1658    5910 
   Size:       1658    4252
 
AccI GT'mk_AC

Cuts at:      0     431    1438    1702    3090    3584    5557    5702    5910 
   Size:        431    1007     264    1388     494    1973     145     208

  Fragments arranged by size: 

                1973    1388    1007     494     431     264     208     145
            
 
Acc65I G'GTAC_C

Cuts at:      0     557    5910 
   Size:        557    5353
 
AclI AA'CG_TT

Cuts at:      0    3493    5910 
   Size:       3493    2417
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112    1036    4653    5910 
   Size:        112     924    3617    1257

  Fragments arranged by size: 

                3617    1257     924     112
 
AflII C'TTAA_G

Cuts at:      0    2725    3370    5910 
   Size:       2725     645    2540

  Fragments arranged by size: 

                2725    2540     645
 
AflIII A'CryG_T

Cuts at:      0     217     426    1346    5910 
   Size:        217     209     920    4564

  Fragments arranged by size: 

                4564     920     217     209
 
AhdI GACnn_n'nnGTC

Cuts at:      0     239    4252    5910 
   Size:        239    4013    1658

  Fragments arranged by size: 

                4013    1658     239
 
AleI CACnn'nnGTG

Cuts at:      0       8     949    1349    1630    1879    3553    5910 
   Size:          8     941     400     281     249    1674    2357

  Fragments arranged by size: 

                2357    1674     941     400     281     249       8
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     821    1300    4452    5910 
   Size:        821     479    3152    1458

  Fragments arranged by size: 

                3152    1458     821     479
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     472     504     561     593    5910 
   Size:        472      32      57      32    5317

  Fragments arranged by size: 

                5317     472      57      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1524    1551    2715    5910 
   Size:       1524      27    1164    3195

  Fragments arranged by size: 

                3195    1524    1164      27
 
ApaI G_GGCC'C

Cuts at:      0    2787    5910 
   Size:       2787    3123
 
ApoI r'AATT_y

Cuts at:      0    1639    2863    3979    5910 
   Size:       1639    1224    1116    1931

  Fragments arranged by size: 

                1931    1639    1224    1116
 
AvaI C'yCGr_G

Cuts at:      0      21     177    2243    3247    3443    4713    5910 
   Size:         21     156    2066    1004     196    1270    1197

  Fragments arranged by size: 

                2066    1270    1197    1004     196     156      21
 
BamHI G'GATC_C

Cuts at:      0     569    4001    5910 
   Size:        569    3432    1909

  Fragments arranged by size: 

                3432    1909     569
 
BanI G'GyrC_C

Cuts at:      0     557    1243    4585    4759    5910 
   Size:        557     686    3342     174    1151

  Fragments arranged by size: 

                3342    1151     686     557     174
 
BanII G_rGCy'C

Cuts at:      0     719     805    2180    2540    2787    3267    3380    4711 
   Size:        719      86    1375     360     247     480     113    1331

Cuts at:   4711    4805    5910 
   Size:         94    1105

  Fragments arranged by size: 

                1375    1331    1105     719     480     360     247     113
                  94      86
 
BbeI G_GCGC'C

Cuts at:      0    1247    4589    5910 
   Size:       1247    3342    1321

  Fragments arranged by size: 

                3342    1321    1247
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     310    1650    1844    2684    3414    3840    5910 
   Size:        310    1340     194     840     730     426    2070

  Fragments arranged by size: 

                2070    1340     840     730     426     310     194
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1816    2566    5910 
   Size:       1816     750    3344

  Fragments arranged by size: 

                3344    1816     750
 
BclI T'GATC_A

Cuts at:      0    1904    2591    5910 
   Size:       1904     687    3319

  Fragments arranged by size: 

                3319    1904     687
 
BglI GCCn_nnn'nGGC

Cuts at:      0     254     545    2690    5910 
   Size:        254     291    2145    3220

  Fragments arranged by size: 

                3220    2145     291     254
 
BglII A'GATC_T

Cuts at:      0     668    4091    5910 
   Size:        668    3423    1819

  Fragments arranged by size: 

                3423    1819     668
 
BlpI GC'TnA_GC

Cuts at:      0    2827    5910 
   Size:       2827    3083
 
Bme1580I G_kGCm'C

Cuts at:      0     767    1142    1939    2787    3365    5910 
   Size:        767     375     797     848     578    2545

  Fragments arranged by size: 

                2545     848     797     767     578     375
 
BmgBI CAC'GTC

Cuts at:      0    1706    1940    2823    4053    4073    4152    4311    5910 
   Size:       1706     234     883    1230      20      79     159    1599

  Fragments arranged by size: 

                1706    1599    1230     883     234     159      79      20
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1298    4514    4592    4637    5910 
   Size:       1298    3216      78      45    1273

  Fragments arranged by size: 

                3216    1298    1273      78      45
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4824    5910 
   Size:       4824    1086
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     913    1546    1684    2194    2936    3502    4027    4153 
   Size:        913     633     138     510     742     566     525     126

Cuts at:   4153    5681    5910 
   Size:       1528     229

  Fragments arranged by size: 

                1528     913     742     633     566     525     510     229
                 138     126
 
Bpu10I CC'TnA_GC

Cuts at:      0    2896    5910 
   Size:       2896    3014
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    4716    5910 
   Size:        991    3725    1194

  Fragments arranged by size: 

                3725    1194     991
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4365    4700    5910 
   Size:       4365     335    1210

  Fragments arranged by size: 

                4365    1210     335
 
BsaAI yAC'GTr

Cuts at:      0     288    5910 
   Size:        288    5622
 
BsaBI GATnn'nnATC

Cuts at:      0    2338    3961    4237    5910 
   Size:       2338    1623     276    1673

  Fragments arranged by size: 

                2338    1673    1623     276
 
BsaHI Gr'CG_yC

Cuts at:      0     999    1244    1655    4586    5910 
   Size:        999     245     411    2931    1324

  Fragments arranged by size: 

                2931    1324     999     411     245
 
BsaWI w'CCGG_w

Cuts at:      0      81    2314    2411    3668    5910 
   Size:         81    2233      97    1257    2242

  Fragments arranged by size: 

                2242    2233    1257      97      81
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3202    3232    4141    4171    5805    5835    5910 
   Size:       3202      30     909      30    1634      30      75

  Fragments arranged by size: 

                3202    1634     909      75      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     487     706     916    2029    2250    3181    3826    5910 
   Size:        487     219     210    1113     221     931     645    2084

  Fragments arranged by size: 

                2084    1113     931     645     487     221     219     210
            
 
BseYI C'CCAG_C

Cuts at:      0     705    3354    4104    4377    4771    4921    4942    4963 
   Size:        705    2649     750     273     394     150      21      21

Cuts at:   4963    4984    5005    5026    5089    5110    5131    5152    5194 
   Size:         21      21      21      63      21      21      21      42

Cuts at:   5194    5278    5299    5383    5467    5479    5488    5542    5910 
   Size:         84      21      84      84      12       9      54     368

  Fragments arranged by size: 

                2649     750     705     394     368     273     150      84
                  84      84      63      54      42      21      21      21
                  21      21      21      21      21      21      12       9
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242    1375    1633    2680    2995    5910 
   Size:        242    1133     258    1047     315    2915

  Fragments arranged by size: 

                2915    1133    1047     315     258     242
 
BsiEI CG_ry'CG

Cuts at:      0    4397    5910 
   Size:       4397    1513
 
BsiHKAI G_wGCw'C

Cuts at:      0    2897    3380    3550    3770    5910 
   Size:       2897     483     170     220    2140

  Fragments arranged by size: 

                2897    2140     483     220     170
 
BsiWI C'GTAC_G

Cuts at:      0    4062    5910 
   Size:       4062    1848
 
BsmI GAATG_Cn'

Cuts at:      0    1424    2228    2970    5910 
   Size:       1424     804     742    2940

  Fragments arranged by size: 

                2940    1424     804     742
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     127    1522    1646    4003    5090    5910 
   Size:        127    1395     124    2357    1087     820

  Fragments arranged by size: 

                2357    1395    1087     820     127     124
 
Bsp1286I G_dGCh'C

Cuts at:      0     719     767     805    1142    1939    2180    2540    2787 
   Size:        719      48      38     337     797     241     360     247

Cuts at:   2787    2897    3267    3365    3380    3550    3770    4711    4805 
   Size:        110     370      98      15     170     220     941      94

Cuts at:   4805    5910 
   Size:       1105

  Fragments arranged by size: 

                1105     941     797     719     370     360     337     247
                 241     220     170     110      98      94      48      38
                  15
 
BspEI T'CCGG_A

Cuts at:      0      81    2314    5910 
   Size:         81    2233    3596

  Fragments arranged by size: 

                3596    2233      81
 
BspHI T'CATG_A

Cuts at:      0    1983    3782    4280    5910 
   Size:       1983    1799     498    1630

  Fragments arranged by size: 

                1983    1799    1630     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1325    1520    2954    3017    3140    3300    4800    5910 
   Size:       1325     195    1434      63     123     160    1500    1110

  Fragments arranged by size: 

                1500    1434    1325    1110     195     160     123      63
            
 
BsrBI CCG'CTC

Cuts at:      0     198     771    1145    1668    2859    2871    3442    4137 
   Size:        198     573     374     523    1191      12     571     695

Cuts at:   4137    4808    5910 
   Size:        671    1102

  Fragments arranged by size: 

                1191    1102     695     671     573     571     523     374
                 198      12
 
BsrDI GCAATG_nn'

Cuts at:      0     882     905    1148    2075    2351    3236    5910 
   Size:        882      23     243     927     276     885    2674

  Fragments arranged by size: 

                2674     927     885     882     276     243      23
 
BsrFI r'CCGG_y

Cuts at:      0    1324    1376    1728    2485    4396    4495    4630    5910 
   Size:       1324      52     352     757    1911      99     135    1280

  Fragments arranged by size: 

                1911    1324    1280     757     352     135      99      52
            
 
BsrGI T'GTAC_A

Cuts at:      0    3920    5910 
   Size:       3920    1990
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4406    4576    5910 
   Size:       4406     170    1334

  Fragments arranged by size: 

                4406    1334     170
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     951    1884    3024    3328    4527    5910 
   Size:        951     933    1140     304    1199    1383

  Fragments arranged by size: 

                1383    1199    1140     951     933     304
 
BstYI r'GATC_y

Cuts at:      0     519     569     638     668    1541    4001    4091    4454 
   Size:        519      50      69      30     873    2460      90     363

Cuts at:   4454    5910 
   Size:       1456

  Fragments arranged by size: 

                2460    1456     873     519     363      90      69      50
                  30
 
BstZ17I GTA'TAC

Cuts at:      0    5558    5910 
   Size:       5558     352
 
Bsu36I CC'TnA_GG

Cuts at:      0     506    5910 
   Size:        506    5404
 
BtgI C'CryG_G

Cuts at:      0     540     947    1432    1556    2457    2519    2600    3680 
   Size:        540     407     485     124     901      62      81    1080

Cuts at:   3680    4526    5910 
   Size:        846    1384

  Fragments arranged by size: 

                1384    1080     901     846     540     485     407     124
                  81      62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3806    5910 
   Size:       3806    2104
 
BtsI GCAGTG_nn'

Cuts at:      0    1637    5910 
   Size:       1637    4273
 
DraIII CAC_nnn'GTG

Cuts at:      0    3146    4336    4756    5910 
   Size:       3146    1190     420    1154

  Fragments arranged by size: 

                3146    1190    1154     420
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2551    2926    5910 
   Size:       2551     375    2984

  Fragments arranged by size: 

                2984    2551     375
 
EaeI y'GGCC_r

Cuts at:      0     204     246     255     269     730     941    1553    1730 
   Size:        204      42       9      14     461     211     612     177

Cuts at:   1730    4224    4235    4394    5910 
   Size:       2494      11     159    1516

  Fragments arranged by size: 

                2494    1516     612     461     211     204     177     159
                  42      14      11       9
 
EagI C'GGCC_G

Cuts at:      0    4394    5910 
   Size:       4394    1516
 
EarI CTCTTCn'nnn_

Cuts at:      0    2005    3124    3812    5910 
   Size:       2005    1119     688    2098

  Fragments arranged by size: 

                2098    2005    1119     688
 
EcoICRI GAG'CTC

Cuts at:      0    3378    5910 
   Size:       3378    2532
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     913    1036    1546    1684    2194    2936    3502 
   Size:        112     801     123     510     138     510     742     566

Cuts at:   3502    4027    4153    4653    5681    5910 
   Size:        525     126     500    1028     229

  Fragments arranged by size: 

                1028     801     742     566     525     510     510     500
                 229     138     126     123     112
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     442    2044    2815    4751    5910 
   Size:        442    1602     771    1936    1159

  Fragments arranged by size: 

                1936    1602    1159     771     442
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1719    2783    2784    2808    3987    4128    4718    4726 
   Size:       1719    1064       1      24    1179     141     590       8

Cuts at:   4726    5910 
   Size:       1184

  Fragments arranged by size: 

                1719    1184    1179    1064     590     141      24       8
                   1
 
EcoRI G'AATT_C

Cuts at:      0    3979    5910 
   Size:       3979    1931
 
EcoRV GAT'ATC

Cuts at:      0     384    5910 
   Size:        384    5526
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1788    2778    4308    5910 
   Size:       1788     990    1530    1602

  Fragments arranged by size: 

                1788    1602    1530     990
 
FspI TGC'GCA

Cuts at:      0      46    1219    1636    3242    3275    5910 
   Size:         46    1173     417    1606      33    2635

  Fragments arranged by size: 

                2635    1606    1173     417      46      33
 
HaeII r_GCGC'y

Cuts at:      0    1247    4589    5910 
   Size:       1247    3342    1321

  Fragments arranged by size: 

                3342    1321    1247
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     225     257     846     878    1398    1430    1526    1558 
   Size:        225      32     589      32     520      32      96      32

Cuts at:   1558    2406    2438    3202    3234    4238    4270    5910 
   Size:        848      32     764      32    1004      32    1640

  Fragments arranged by size: 

                1640    1004     848     764     589     520     225      96
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1703    3091    4257    5910 
   Size:       1703    1388    1166    1653

  Fragments arranged by size: 

                1703    1653    1388    1166
 
HindIII A'AGCT_T

Cuts at:      0     157    5910 
   Size:        157    5753
 
KasI G'GCGC_C

Cuts at:      0    1243    4585    5910 
   Size:       1243    3342    1325

  Fragments arranged by size: 

                3342    1325    1243
 
KpnI G_GTAC'C

Cuts at:      0     561    5910 
   Size:        561    5349
 
MfeI C'AATT_G

Cuts at:      0    1800    5910 
   Size:       1800    4110
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2033    2343    2759    2834    3029    3893    4915    5272 
   Size:       2033     310     416      75     195     864    1022     357

Cuts at:   5272    5398    5470    5910 
   Size:        126      72     440

  Fragments arranged by size: 

                2033    1022     864     440     416     357     310     195
                 126      75      72
 
MscI TGG'CCA

Cuts at:      0     248     257     271     943    1555    4226    4237    5910 
   Size:        248       9      14     672     612    2671      11    1673

  Fragments arranged by size: 

                2671    1673     672     612     248      14      11       9
            
 
MslI CAynn'nnrTG

Cuts at:      0       8     614     949    1349    1630    1829    1879    2165 
   Size:          8     606     335     400     281     199      50     286

Cuts at:   2165    3022    3301    3553    4498    5910 
   Size:        857     279     252     945    1412

  Fragments arranged by size: 

                1412     945     857     606     400     335     286     281
                 279     252     199      50       8
 
MspA1I CmG'CkG

Cuts at:      0      42     209     867     895    1023    1945    2583    2664 
   Size:         42     167     658      28     128     922     638      81

Cuts at:   2664    4386    4664    5910 
   Size:       1722     278    1246

  Fragments arranged by size: 

                1722    1246     922     658     638     278     167     128
                  81      42      28
 
NaeI GCC'GGC

Cuts at:      0    1730    2487    4398    4632    5910 
   Size:       1730     757    1911     234    1278

  Fragments arranged by size: 

                1911    1730    1278     757     234
 
NarI GG'CG_CC

Cuts at:      0    1244    4586    5910 
   Size:       1244    3342    1324

  Fragments arranged by size: 

                3342    1324    1244
 
NcoI C'CATG_G

Cuts at:      0     540     947    1432    1556    2519    2600    3680    4526 
   Size:        540     407     485     124     963      81    1080     846

Cuts at:   4526    5910 
   Size:       1384

  Fragments arranged by size: 

                1384    1080     963     846     540     485     407     124
                  81
 
NdeI CA'TA_TG

Cuts at:      0     457    5910 
   Size:        457    5453
 
NgoMIV G'CCGG_C

Cuts at:      0    1728    2485    4396    4630    5910 
   Size:       1728     757    1911     234    1280

  Fragments arranged by size: 

                1911    1728    1280     757     234
 
NspI r_CATG'y

Cuts at:      0      38     221     430    1350    3876    3927    4503    5910 
   Size:         38     183     209     920    2526      51     576    1407

  Fragments arranged by size: 

                2526    1407     920     576     209     183      51      38
            
 
PasI CC'CwG_GG

Cuts at:      0    2185    4621    5910 
   Size:       2185    2436    1289

  Fragments arranged by size: 

                2436    2185    1289
 
PciI A'CATG_T

Cuts at:      0     217     426    1346    5910 
   Size:        217     209     920    4564

  Fragments arranged by size: 

                4564     920     217     209
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1551    2520    2715    4233    5910 
   Size:       1551     969     195    1518    1677

  Fragments arranged by size: 

                1677    1551    1518     969     195
 
PfoI T'CCnGG_A

Cuts at:      0    1544    1661    4004    5910 
   Size:       1544     117    2343    1906

  Fragments arranged by size: 

                2343    1906    1544     117
 
PmlI CAC'GTG

Cuts at:      0     288    5910 
   Size:        288    5622
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2740    2772    3208    3240    5910 
   Size:       2740      32     436      32    2670

  Fragments arranged by size: 

                2740    2670     436      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1719    2808    4718    4726    5910 
   Size:       1719    1089    1910       8    1184

  Fragments arranged by size: 

                1910    1719    1184    1089       8
 
PspOMI G'GGCC_C

Cuts at:      0    2783    5910 
   Size:       2783    3127
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1219    1251    5910 
   Size:       1219      32    4659

  Fragments arranged by size: 

                4659    1219      32
 
PstI C_TGCA'G

Cuts at:      0    1322    2804    3896    3995    5910 
   Size:       1322    1482    1092      99    1915

  Fragments arranged by size: 

                1915    1482    1322    1092      99
 
PvuII CAG'CTG

Cuts at:      0     867    1945    2664    4386    5910 
   Size:        867    1078     719    1722    1524

  Fragments arranged by size: 

                1722    1524    1078     867     719
 
SacI G_AGCT'C

Cuts at:      0    3380    5910 
   Size:       3380    2530
 
SalI G'TCGA_C

Cuts at:      0    1701    3089    5910 
   Size:       1701    1388    2821

  Fragments arranged by size: 

                2821    1701    1388
 
SanDI GG'GwC_CC

Cuts at:      0    4718    5910 
   Size:       4718    1192
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3124    5910 
   Size:       3124    2786
 
SbfI CC_TGCA'GG

Cuts at:      0    3896    3995    5910 
   Size:       3896      99    1915

  Fragments arranged by size: 

                3896    1915      99
 
ScaI AGT'ACT

Cuts at:      0    5578    5620    5725    5767    5910 
   Size:       5578      42     105      42     143

  Fragments arranged by size: 

                5578     143     105      42      42
 
SexAI A'CCwGG_T

Cuts at:      0    1127    1788    2651    2810    4752    5910 
   Size:       1127     661     863     159    1942    1158

  Fragments arranged by size: 

                1942    1158    1127     863     661     159
 
SfcI C'TryA_G

Cuts at:      0    1318    2800    3892    3991    4905    5283    5346    5472 
   Size:       1318    1482    1092      99     914     378      63     126

Cuts at:   5472    5493    5859    5910 
   Size:         21     366      51

  Fragments arranged by size: 

                1482    1318    1092     914     378     366     126      99
                  63      51      21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     254    5910 
   Size:        254    5656
 
SfoI GGC'GCC

Cuts at:      0    1245    4587    5910 
   Size:       1245    3342    1323

  Fragments arranged by size: 

                3342    1323    1245
 
SmaI CCC'GGG

Cuts at:      0    3249    4715    5910 
   Size:       3249    1466    1195

  Fragments arranged by size: 

                3249    1466    1195
 
SmlI C'TyrA_G

Cuts at:      0    1006    2725    3370    3443    4731    5910 
   Size:       1006    1719     645      73    1288    1179

  Fragments arranged by size: 

                1719    1288    1179    1006     645      73
 
SpeI A'CTAG_T

Cuts at:      0    1212    5314    5910 
   Size:       1212    4102     596

  Fragments arranged by size: 

                4102    1212     596
 
SphI G_CATG'C

Cuts at:      0      38    5910 
   Size:         38    5872
 
SspI AAT'ATT

Cuts at:      0    5599    5910 
   Size:       5599     311
 
StuI AGG'CCT

Cuts at:      0    3190    5910 
   Size:       3190    2720
 
StyI C'CwwG_G

Cuts at:      0     398     540     947    1432    1556    1877    2519    2600 
   Size:        398     142     407     485     124     321     642      81

Cuts at:   2600    3282    3461    3680    4526    4555    5910 
   Size:        682     179     219     846      29    1355

  Fragments arranged by size: 

                1355     846     682     642     485     407     398     321
                 219     179     142     124      81      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1837    2511    3313    3346    3564    4519    4533    4935 
   Size:       1837     674     802      33     218     955      14     402

Cuts at:   4935    4956    4989    5052    5145    5199    5220    5241    5292 
   Size:         21      33      63      93      54      21      21      51

Cuts at:   5292    5313    5397    5481    5523    5619    5661    5910 
   Size:         21      84      84      42      96      42     249

  Fragments arranged by size: 

                1837     955     802     674     402     249     218      96
                  93      84      84      63      54      51      42      42
                  33      33      21      21      21      21      14
 
TatI w'GTAC_w

Cuts at:      0    1250    3920    5513    5576    5618    5723    5765    5910 
   Size:       1250    2670    1593      63      42     105      42     145

  Fragments arranged by size: 

                2670    1593    1250     145     105      63      42      42
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1384    1416    3121    3153    3457    3489    4141    4173 
   Size:       1384      32    1705      32     304      32     652      32

Cuts at:   4173    4551    4583    5910 
   Size:        378      32    1327

  Fragments arranged by size: 

                1705    1384    1327     652     378     304      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     351     547    1884    2652    3024    4083    5910 
   Size:        351     196    1337     768     372    1059    1827

  Fragments arranged by size: 

                1827    1337    1059     768     372     351     196
 
XhoI C'TCGA_G

Cuts at:      0    3443    5910 
   Size:       3443    2467
 
XmaI C'CCGG_G

Cuts at:      0    3247    4713    5910 
   Size:       3247    1466    1197

  Fragments arranged by size: 

                3247    1466    1197
 
XmnI GAAnn'nnTTC

Cuts at:      0    2755    2977    3316    5910 
   Size:       2755     222     339    2594

  Fragments arranged by size: 

                2755    2594     339     222
 
ZraI GAC'GTC

Cuts at:      0    1656    5910 
   Size:       1656    4254
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI   DraIII
     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PsrI     PstI    PvuII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AgeI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BbvCI     BcgI    BfrBI     BmtI   BssHII    BssSI    BstBI   BstEII
     ClaI    CspCI     DraI     EciI     FseI    FspAI     HpaI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI    PshAI     PsiI
    PspXI     PvuI    RsrII    SacII     SgfI    SgrAI    SnaBI     SrfI
     SwaI  Tth111I     XbaI
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