Restriction map for pF1KE2450
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AluI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoT14I     EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        324  GGTACC               G^GTACC
AcyI          579  GGCGCC               G[GA]^CG[CT]C
Alw44I        640  GTGCAC               G^TGCAC
ApaLI         640  GTGCAC               G^TGCAC
Asp718I       324  GGTACC               G^GTACC
BanII         100  GAGCCC               G[GA]GC[CT]^C
BbeI          579  GGCGCC               GGCGC^C
BclI          359  TGATCA               T^GATCA
BfaI           49  CTAG                 C^TAG
BglI          629  GCCTGGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          766  CTGGAG               CTGGAG(16/14)
BsaHI         579  GGCGCC               G[GA]^CG[CT]C
Bsp1407I      611  TGTACA               T^GTACA
BsrDI         783  CATTGC               GCAATG(2/0)
BsrGI         611  TGTACA               T^GTACA
BssSI         289  CTCGTG               CACGAG(-5/-1)
BstYI         761  AGATCC               [GA]^GATC[CT]
DdeI          296  CTGAG                C^T[ACGT]AG
EarI          492  GAAGAG               CTCTTC(1/4)
EcoNI         765  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV          13  GATATC               GAT^ATC
FbaI          359  TGATCA               T^GATCA
HgaI          144  GACGC                GACGC(5/10)
Hin1I         579  GGCGCC               G[GA]^CG[CT]C
KasI          579  GGCGCC               G^GCGCC
KpnI          324  GGTACC               GGTAC^C
Ksp632I       492  GAAGAG               CTCTTC(1/4)
KspI          624  CCGCGG               CCGC^GG
MaeI           49  CTAG                 C^TAG
MflI          761  AGATCC               [GA]^GATC[CT]
MslI          912  CATCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          579  GGCGCC               GG^CGCC
PshAI         447  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         624  CCGCGG               CCGC^GG
ScaI          341  AGTACT               AGT^ACT
SfoI          579  GGCGCC               GGC^GCC
SmaI          499  CCCGGG               CCC^GGG
SspBI         611  TGTACA               T^GTACA
XcmI          356  CCATGATCATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         761  AGATCC               [GA]^GATC[CT]
XmaCI         499  CCCGGG               C^CCGGG
XmaI          499  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         474  CGCG                 CG^CG
              625  CGCG                
AlwI          171  GATCC                GGATC(4/5)
              762  GATCC               
AvaI          499  CCCGGG               C^[CT]CG[GA]G
              736  CCCGAG              
BalI          554  TGGCCA               TGG^CCA
              755  TGGCCA              
BanI          324  GGTACC               G^G[CT][GA]CC
              579  GGCGCC              
BbsI          277  GTCTTC               GAAGAC(2/6)
              886  GTCTTC              
BpuAI         277  GTCTTC               GAAGAC(2/6)
              886  GTCTTC              
BseRI          69  GAGGAG               GAGGAG(10/8)
              769  GAGGAG              
BsgI          718  CTGCAC               GTGCAG(16/14)
              900  CTGCAC              
BsoBI         499  CCCGGG               C^[CT]CG[GA]G
              736  CCCGAG              
BspMI         164  ACCTGC               ACCTGC(4/8)
              605  ACCTGC              
BstAPI        683  GCATGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              711  GCACCTCCTGC         
BstUI         474  CGCG                 CG^CG
              625  CGCG                
BstXI         758  CCAAGATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              929  CCAACTTCGTGG        
DraII          44  GGGACCT              [GA]G^G[ACGT]CC[CT]
              743  GGGACCC             
DsaI          575  CCGTGG               C^C[GA][CT]GG
              624  CCGCGG              
EcoO109I       44  GGGACCT              [GA]G^G[ACGT]CC[CT]
              743  GGGACCC             
FauI          307  CCCGC                CCCGC(4/6)
              589  CCCGC               
MluNI         554  TGGCCA               TGG^CCA
              755  TGGCCA              
MscI          554  TGGCCA               TGG^CCA
              755  TGGCCA              
MvnI          474  CGCG                 CG^CG
              625  CGCG                
PleI          661  GACTC                GAGTC(4/5)
              452  GAGTC               
PpuMI          44  GGGACCT              [GA]G^G[AT]CC[CT]
              743  GGGACCC             
SfaNI         435  GATGC                GCATC(5/9)
              911  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AspHI       AvaII       BbvI        
BcnI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DpnII       EaeI        EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        TfiI        TseI        Tsp45I      
TspRI       
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