Restriction map for pF1KE2450
 (Linear) (Six-base) MAPSORT of: KIEE2450.seq  Check: 1311  from: 1  to: 963



KIEE2450.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0     324     963 
   Size:        324     639
 
ApaLI G'TGCA_C

Cuts at:      0     640     963 
   Size:        640     323
 
AvaI C'yCGr_G

Cuts at:      0     499     736     963 
   Size:        499     237     227

  Fragments arranged by size: 

                 499     237     227
 
BanI G'GyrC_C

Cuts at:      0     324     579     963 
   Size:        324     255     384

  Fragments arranged by size: 

                 384     324     255
 
BanII G_rGCy'C

Cuts at:      0     104     963 
   Size:        104     859
 
BbeI G_GCGC'C

Cuts at:      0     583     963 
   Size:        583     380
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     270     879     963 
   Size:        270     609      84

  Fragments arranged by size: 

                 609     270      84
 
BclI T'GATC_A

Cuts at:      0     359     963 
   Size:        359     604
 
BglI GCCn_nnn'nGGC

Cuts at:      0     635     963 
   Size:        635     328
 
Bme1580I G_kGCm'C

Cuts at:      0     644     963 
   Size:        644     319
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     333     963 
   Size:        333     630
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     787     963 
   Size:        787     176
 
BsaHI Gr'CG_yC

Cuts at:      0     580     963 
   Size:        580     383
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     931     961     963 
   Size:        931      30       2

  Fragments arranged by size: 

                 931      30       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      84     784     963 
   Size:         84     700     179

  Fragments arranged by size: 

                 700     179      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703     885     963 
   Size:        703     182      78

  Fragments arranged by size: 

                 703     182      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     644     713     963 
   Size:        185     459      69     250

  Fragments arranged by size: 

                 459     250     185      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     104     185     644     713     963 
   Size:        104      81     459      69     250

  Fragments arranged by size: 

                 459     250     104      81      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     173     614     963 
   Size:        173     441     349

  Fragments arranged by size: 

                 441     349     173
 
BsrDI GCAATG_nn'

Cuts at:      0     782     963 
   Size:        782     181
 
BsrGI T'GTAC_A

Cuts at:      0     611     963 
   Size:        611     352
 
BssSI C'ACGA_G

Cuts at:      0     289     963 
   Size:        289     674
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     689     717     963 
   Size:        689      28     246

  Fragments arranged by size: 

                 689     246      28
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     765     936     963 
   Size:        765     171      27

  Fragments arranged by size: 

                 765     171      27
 
BstYI r'GATC_y

Cuts at:      0     761     963 
   Size:        761     202
 
BtgI C'CryG_G

Cuts at:      0     575     624     963 
   Size:        575      49     339

  Fragments arranged by size: 

                 575     339      49
 
EaeI y'GGCC_r

Cuts at:      0     554     678     755     963 
   Size:        554     124      77     208

  Fragments arranged by size: 

                 554     208     124      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     487     963 
   Size:        487     476
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     180     963 
   Size:        180     783
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     787     963 
   Size:        787     176
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     769     963 
   Size:        769     194
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     744     963 
   Size:         45     699     219

  Fragments arranged by size: 

                 699     219      45
 
EcoRV GAT'ATC

Cuts at:      0      15     963 
   Size:         15     948
 
HaeII r_GCGC'y

Cuts at:      0     546     575     583     963 
   Size:        546      29       8     380

  Fragments arranged by size: 

                 546     380      29       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     909     931     941     963 
   Size:        909      22      10      22

  Fragments arranged by size: 

                 909      22      22      10
 
KasI G'GCGC_C

Cuts at:      0     579     963 
   Size:        579     384
 
KpnI G_GTAC'C

Cuts at:      0     328     963 
   Size:        328     635
 
MscI TGG'CCA

Cuts at:      0     556     757     963 
   Size:        556     201     206

  Fragments arranged by size: 

                 556     206     201
 
MslI CAynn'nnrTG

Cuts at:      0     916     963 
   Size:        916      47
 
MspA1I CmG'CkG

Cuts at:      0     540     626     800     963 
   Size:        540      86     174     163

  Fragments arranged by size: 

                 540     174     163      86
 
NarI GG'CG_CC

Cuts at:      0     580     963 
   Size:        580     383
 
PasI CC'CwG_GG

Cuts at:      0     246     963 
   Size:        246     717
 
PfoI T'CCnGG_A

Cuts at:      0     764     963 
   Size:        764     199
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     823     855     963 
   Size:        823      32     108

  Fragments arranged by size: 

                 823     108      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      45     744     963 
   Size:         45     699     219

  Fragments arranged by size: 

                 699     219      45
 
PshAI GACnn'nnGTC

Cuts at:      0     451     963 
   Size:        451     512
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     595     627     963 
   Size:        595      32     336

  Fragments arranged by size: 

                 595     336      32
 
PstI C_TGCA'G

Cuts at:      0     225     404     550     829     963 
   Size:        225     179     146     279     134

  Fragments arranged by size: 

                 279     225     179     146     134
 
SacII CC_GC'GG

Cuts at:      0     627     963 
   Size:        627     336
 
SanDI GG'GwC_CC

Cuts at:      0     744     963 
   Size:        744     219
 
ScaI AGT'ACT

Cuts at:      0     343     963 
   Size:        343     620
 
SfcI C'TryA_G

Cuts at:      0     221     400     546     825     963 
   Size:        221     179     146     279     138

  Fragments arranged by size: 

                 279     221     179     146     138
 
SfoI GGC'GCC

Cuts at:      0     581     963 
   Size:        581     382
 
SmaI CCC'GGG

Cuts at:      0     501     963 
   Size:        501     462
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     873     963 
   Size:        873      90
 
TatI w'GTAC_w

Cuts at:      0     341     611     963 
   Size:        341     270     352

  Fragments arranged by size: 

                 352     341     270
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     850     882     963 
   Size:        850      32      81

  Fragments arranged by size: 

                 850      81      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     363     963 
   Size:        363     600
 
XmaI C'CCGG_G

Cuts at:      0     499     963 
   Size:        499     464
 

 Enzymes that do cut:

   Acc65I    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BclI
     BglI Bme1580I     BmrI     BpmI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstXI
    BstYI     BtgI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRV    HaeII    Hin4I     KasI     KpnI     MscI     MslI   MspA1I
     NarI     PasI     PfoI     PpiI    PpuMI    PshAI     PsrI     PstI
    SacII    SanDI     ScaI     SfcI     SfoI     SmaI    TaqII     TatI
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI     PsiI   PspOMI    PspXI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI    SexAI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XhoI     XmnI
     ZraI
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