Restriction map for pF1KE2065
 (Linear) (Six-base) MAPSORT of: KIEE2065.seq  Check: 9602  from: 1  to: 2157



KIEE2065.orf

 With 182 enzymes: SgfI * 

                        July 14, 2018 20:54  ..

AatII G_ACGT'C

Cuts at:      0     131    2157 
   Size:        131    2026
 
Acc65I G'GTAC_C

Cuts at:      0     223    2157 
   Size:        223    1934
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     225     334     755    1228    1690    2157 
   Size:         91     134     109     421     473     462     467

  Fragments arranged by size: 

                 473     467     462     421     134     109      91
 
AflII C'TTAA_G

Cuts at:      0     204    2157 
   Size:        204    1953
 
AlwNI CAG_nnn'CTG

Cuts at:      0      45      96     470    2157 
   Size:         45      51     374    1687

  Fragments arranged by size: 

                1687     374      51      45
 
AvaI C'yCGr_G

Cuts at:      0    1498    1545    2157 
   Size:       1498      47     612

  Fragments arranged by size: 

                1498     612      47
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     215     248    2157 
   Size:        215      33    1909

  Fragments arranged by size: 

                1909     215      33
 
BamHI G'GATC_C

Cuts at:      0     876    2081    2157 
   Size:        876    1205      76

  Fragments arranged by size: 

                1205     876      76
 
BanI G'GyrC_C

Cuts at:      0      30     223    2157 
   Size:         30     193    1934

  Fragments arranged by size: 

                1934     193      30
 
BanII G_rGCy'C

Cuts at:      0     488    1517    2157 
   Size:        488    1029     640

  Fragments arranged by size: 

                1029     640     488
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1216    2157 
   Size:       1216     941
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1489    1523    2157 
   Size:       1489      34     634

  Fragments arranged by size: 

                1489     634      34
 
BclI T'GATC_A

Cuts at:      0     761    2157 
   Size:        761    1396
 
BlpI GC'TnA_GC

Cuts at:      0    1393    2157 
   Size:       1393     764
 
BmgBI CAC'GTC

Cuts at:      0    1932    2157 
   Size:       1932     225
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     311    1474    2157 
   Size:        311    1163     683

  Fragments arranged by size: 

                1163     683     311
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1418    2124    2157 
   Size:       1418     706      33

  Fragments arranged by size: 

                1418     706      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     694     775    1051    1807    2157 
   Size:        535     159      81     276     756     350

  Fragments arranged by size: 

                 756     535     350     276     159      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     814    1846    2157 
   Size:        814    1032     311

  Fragments arranged by size: 

                1032     814     311
 
BsaBI GATnn'nnATC

Cuts at:      0    1226    2157 
   Size:       1226     931
 
BsaHI Gr'CG_yC

Cuts at:      0     128     740    2157 
   Size:        128     612    1417

  Fragments arranged by size: 

                1417     612     128
 
BsaWI w'CCGG_w

Cuts at:      0    1775    2157 
   Size:       1775     382
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796    2157 
   Size:        766      30    1361

  Fragments arranged by size: 

                1361     766      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1354    1357    1565    2157 
   Size:       1354       3     208     592

  Fragments arranged by size: 

                1354     592     208       3
 
BseYI C'CCAG_C

Cuts at:      0      17      45     463    1492    1911    2157 
   Size:         17      28     418    1029     419     246

  Fragments arranged by size: 

                1029     419     418     246      28      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     729     826     971     995    2157 
   Size:        181     548      97     145      24    1162

  Fragments arranged by size: 

                1162     548     181     145      97      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     488    2157 
   Size:        488    1669
 
Bsp1286I G_dGCh'C

Cuts at:      0     488    1517    2157 
   Size:        488    1029     640

  Fragments arranged by size: 

                1029     640     488
 
BspEI T'CCGG_A

Cuts at:      0    1775    2157 
   Size:       1775     382
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     993    2157 
   Size:        993    1164
 
BsrBI CCG'CTC

Cuts at:      0    1281    2157 
   Size:       1281     876
 
BsrDI GCAATG_nn'

Cuts at:      0     728     931    2157 
   Size:        728     203    1226

  Fragments arranged by size: 

                1226     728     203
 
BstYI r'GATC_y

Cuts at:      0     356     473     493     876    1163    2081    2157 
   Size:        356     117      20     383     287     918      76

  Fragments arranged by size: 

                 918     383     356     287     117      76      20
 
BtsI GCAGTG_nn'

Cuts at:      0    1760    2157 
   Size:       1760     397
 
DraIII CAC_nnn'GTG

Cuts at:      0     335    2157 
   Size:        335    1822
 
EaeI y'GGCC_r

Cuts at:      0     170     428    2157 
   Size:        170     258    1729

  Fragments arranged by size: 

                1729     258     170
 
EarI CTCTTCn'nnn_

Cuts at:      0     241     265    1024    2157 
   Size:        241      24     759    1133

  Fragments arranged by size: 

                1133     759     241      24
 
EcoICRI GAG'CTC

Cuts at:      0     486    2157 
   Size:        486    1671
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     225     334     535     694     755     775    1051 
   Size:         91     134     109     201     159      61      20     276

Cuts at:   1051    1228    1690    1807    2157 
   Size:        177     462     117     350

  Fragments arranged by size: 

                 462     350     276     201     177     159     134     117
                 109      91      61      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     160    1326    2157 
   Size:        160    1166     831

  Fragments arranged by size: 

                1166     831     160
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1719    2157 
   Size:       1719     438
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     637     669    2066    2098    2157 
   Size:        637      32    1397      32      59

  Fragments arranged by size: 

                1397     637      59      32      32
 
HincII GTy'rAC

Cuts at:      0     801     885     956    1110    2157 
   Size:        801      84      71     154    1047

  Fragments arranged by size: 

                1047     801     154      84      71
 
HpaI GTT'AAC

Cuts at:      0    1110    2157 
   Size:       1110    1047
 
KpnI G_GTAC'C

Cuts at:      0     227    2157 
   Size:        227    1930
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1170    2157 
   Size:       1170     987
 
MscI TGG'CCA

Cuts at:      0     172    2157 
   Size:        172    1985
 
MslI CAynn'nnrTG

Cuts at:      0     388    2157 
   Size:        388    1769
 
MspA1I CmG'CkG

Cuts at:      0      21    1314    1473    2157 
   Size:         21    1293     159     684

  Fragments arranged by size: 

                1293     684     159      21
 
PasI CC'CwG_GG

Cuts at:      0    1385    2157 
   Size:       1385     772
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     470    1041    2157 
   Size:        470     571    1116

  Fragments arranged by size: 

                1116     571     470
 
PfoI T'CCnGG_A

Cuts at:      0      59     260     576    1784    2157 
   Size:         59     201     316    1208     373

  Fragments arranged by size: 

                1208     373     316     201      59
 
PshAI GACnn'nnGTC

Cuts at:      0     572    2157 
   Size:        572    1585
 
PstI C_TGCA'G

Cuts at:      0     137    1583    1767    2157 
   Size:        137    1446     184     390

  Fragments arranged by size: 

                1446     390     184     137
 
PvuII CAG'CTG

Cuts at:      0      21    1314    1473    2157 
   Size:         21    1293     159     684

  Fragments arranged by size: 

                1293     684     159      21
 
SacI G_AGCT'C

Cuts at:      0     488    2157 
   Size:        488    1669
 
SbfI CC_TGCA'GG

Cuts at:      0     137    2157 
   Size:        137    2020
 
SexAI A'CCwGG_T

Cuts at:      0     949    2157 
   Size:        949    1208
 
SfcI C'TryA_G

Cuts at:      0     133    1579    1763    2157 
   Size:        133    1446     184     394

  Fragments arranged by size: 

                1446     394     184     133
 
SmaI CCC'GGG

Cuts at:      0    1547    2157 
   Size:       1547     610
 
SmlI C'TyrA_G

Cuts at:      0     204    2157 
   Size:        204    1953
 
StuI AGG'CCT

Cuts at:      0    1896    2157 
   Size:       1896     261
 
StyI C'CwwG_G

Cuts at:      0     497    1703    2035    2157 
   Size:        497    1206     332     122

  Fragments arranged by size: 

                1206     497     332     122
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1506    1924    2157 
   Size:       1506     418     233

  Fragments arranged by size: 

                1506     418     233
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     691     723     766     798    1936    1968    2157 
   Size:        691      32      43      32    1138      32     189

  Fragments arranged by size: 

                1138     691     189      43      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1929    2033    2157 
   Size:       1929     104     124

  Fragments arranged by size: 

                1929     124     104
 
XmaI C'CCGG_G

Cuts at:      0    1545    2157 
   Size:       1545     612
 
ZraI GAC'GTC

Cuts at:      0     129    2157 
   Size:        129    2028
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI    AflII    AlwNI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI     BcgI     BclI     BlpI    BmgBI     BmrI
     BplI     BpmI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BstYI     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
     FalI    Hin4I   HincII     HpaI     KpnI     MmeI     MscI     MslI
   MspA1I     PasI    PflMI     PfoI    PshAI     PstI    PvuII     SacI
     SbfI    SexAI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BbeI    BbvCI    BciVI    BfrBI     BglI    BglII Bme1580I
     BmtI   Bpu10I    BpuEI    BsaAI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     KasI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI
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