Restriction map for pF1KE9424
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        AluI        AlwNI       AocI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TfiI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII          88  GACGTC               GACGT^C
AfaI          232  GTAC                 GT^AC
AflIII        173  ACGTGT               A^C[GA][CT]GT
Alw44I        421  GTGCAC               G^TGCAC
Aor51HI       268  AGCGCT               AGC^GCT
ApaI          318  GGGCCC               GGGCC^C
ApaLI         421  GTGCAC               G^TGCAC
AscI          195  GGCGCGCC             GG^CGCGCC
AspI          883  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII          41  TGCGCA               TGC^GCA
BbeI          556  GGCGCC               GGCGC^C
BbsI          523  GTCTTC               GAAGAC(2/6)
BclI          671  TGATCA               T^GATCA
BcnI          553  CCCGG                CC^[GC]GG
BglII         611  AGATCT               A^GATCT
BpuAI         523  GTCTTC               GAAGAC(2/6)
BsaAI         346  TACGTG               [CT]AC^GT[GA]
BsaI          810  GGTCTC               GGTCTC(1/5)
BsgI          975  GTGCAG               GTGCAG(16/14)
BsiEI         500  CGGTCG               CG[GA][CT]^CG
BsmI           10  GAATGC               GAATGC(1/-1)
BsrFI         104  ACCGGC               [GA]^CCGG[CT]
BsrI          660  ACTGG                ACTGG(1/-1)
BssSI         615  CTCGTG               CACGAG(-5/-1)
Cfr10I        104  ACCGGC               [GA]^CCGG[CT]
DrdI         1042  GACACATCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      268  AGCGCT               AGC^GCT
FbaI          671  TGATCA               T^GATCA
FspI           41  TGCGCA               TGC^GCA
HinfI         533  GAGTC                G^A[ACGT]TC
KasI          556  GGCGCC               G^GCGCC
MseI         1036  TTAA                 T^TAA
NarI          556  GGCGCC               GG^CGCC
NciI          553  CCCGG                CC^[GC]GG
PflFI         883  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         102  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          533  GAGTC                GAGTC(4/5)
PpuMI         444  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          427  CTGCAG               CTGCA^G
RsaI          232  GTAC                 GT^AC
SexAI         884  ACCTGGT              A^CC[AT]GGT
SfcI          427  CTGCAG               C^T[GA][CT]AG
SfoI          556  GGCGCC               GGC^GCC
StuI          305  AGGCCT               AGG^CCT
Tru9I        1036  TTAA                 T^TAA
Tsp45I        370  GTCAC                ^GT[GC]AC
Tth111I       883  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        102  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          374  CCATCCTCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          756  GATCC                GGATC(4/5)
              864  GATCC               
AspHI         142  GTGCTC               G[AT]GC[AT]^C
              421  GTGCAC              
AvaII         252  GGACC                G^G[AT]CC
              445  GGTCC               
BanI          556  GGCGCC               G^G[CT][GA]CC
              651  GGTGCC              
BanII         318  GGGCCC               G[GA]GC[CT]^C
              831  GAGCCC              
BseRI         802  CTCCTC               GAGGAG(10/8)
              853  CTCCTC              
BsiHKAI       142  GTGCTC               G[AT]GC[AT]^C
              421  GTGCAC              
BsrBI         506  CCGCTC               CCGCTC(-3/-3)
              360  GAGCGG              
BssHII         14  GCGCGC               G^CGCGC
              196  GCGCGC              
BstAPI        170  GCAACGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              407  GCATGGTGTGC         
BstYI         611  AGATCT               [GA]^GATC[CT]
              755  AGATCC              
DdeI          302  CTGAG                C^T[ACGT]AG
              358  CTGAG               
DraII         318  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              444  GGGTCCT             
EcoO109I      318  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              444  GGGTCCT             
HphI          371  TCACC                GGTGA(8/7)
              845  TCACC               
MflI          611  AGATCT               [GA]^GATC[CT]
              755  AGATCC              
SfaNI         416  GCATC                GCATC(5/9)
              706  GCATC               
TaqI          574  TCGA                 T^CGA
             1069  TCGA                
TthHB8I       574  TCGA                 T^CGA
             1069  TCGA                
XhoII         611  AGATCT               [GA]^GATC[CT]
              755  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        ApoI        
BbvI        BmyI        BpmI        BsaHI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DsaI        
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
Ksp632I     KspI        MaeII       MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
SacII       SapI        Sau3AI      Sau96I      ScrFI       
TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com