Restriction map for pF1KE6039
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BpmI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tsp509I     Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwNI         247  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       714  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         331  GGATCC               G^GATCC
BanI          580  GGCACC               G^G[CT][GA]CC
BciVI         617  GTATCC               GTATCC(6/5)
BcnI          351  CCCGG                CC^[GC]GG
BlpI          598  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      598  GCTGAGC              GC^T[ACGT]AGC
BsaI          654  GGTCTC               GGTCTC(1/5)
BseRI         657  CTCCTC               GAGGAG(10/8)
BsmI          718  GCATTC               GAATGC(1/-1)
BsrDI         669  CATTGC               GCAATG(2/0)
CelII         598  GCTGAGC              GC^T[ACGT]AGC
EarI          835  CTCTTC               CTCTTC(1/4)
EcoT14I       437  CCTTGG               C^C[AT][AT]GG
EcoT22I       415  ATGCAT               ATGCA^T
FauI          854  CCCGC                CCCGC(4/6)
HaeIII        435  GGCC                 GG^CC
HapII         352  CCGG                 C^CGG
HgaI          344  GCGTC                GACGC(5/10)
HindIII       169  AAGCTT               A^AGCTT
HinfI         105  GATTC                G^A[ACGT]TC
HpaII         352  CCGG                 C^CGG
Ksp632I       835  CTCTTC               CTCTTC(1/4)
MaeII         586  ACGT                 A^CGT
MspI          352  CCGG                 C^CGG
NciI          351  CCCGG                CC^[GC]GG
NdeI          175  CATATG               CA^TATG
NsiI          415  ATGCAT               ATGCA^T
NspI          857  GCATGC               [GA]CATG^[CT]
PshAI         744  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         502  CAGCTG               CAG^CTG
SmlI          882  CTTGAG               C^T[CT][GA]AG
SphI          857  GCATGC               GCATG^C
SspI          943  AATATT               AAT^ATT
StyI          437  CCTTGG               C^C[AT][AT]GG
TfiI          105  GATTC                G^A[AT]TC
XcmI          137  CCACCATCATTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          714  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          216  GTAC                 GT^AC
              481  GTAC                
AspHI          94  GTGCTC               G[AT]GC[AT]^C
              556  GTGCTC              
BsiHKAI        94  GTGCTC               G[AT]GC[AT]^C
              556  GTGCTC              
BspMI         727  ACCTGC               ACCTGC(4/8)
               37  GCAGGT              
BstEII        750  GGTCACC              G^GT[ACGT]ACC
              849  GGTAACC             
BstPI         750  GGTCACC              G^GT[ACGT]ACC
              849  GGTAACC             
BstXI         140  CCATCATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              470  CCACCACCCTGG        
BstYI         331  GGATCC               [GA]^GATC[CT]
              805  AGATCC              
DraII         548  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              724  GGGACCT             
DraIII        336  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              676  CACGCAGTG           
EcoO109I      548  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              724  GGGACCT             
EcoO65I       750  GGTCACC              G^GT[ACGT]ACC
              849  GGTAACC             
MflI          331  GGATCC               [GA]^GATC[CT]
              805  AGATCC              
MslI           16  CATACCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              774  CATCTTCATG          
MspA1I        219  CCGCTG               C[AC]G^C[GT]G
              502  CAGCTG              
PflMI         246  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              321  CCATGCCCTGG         
PpuMI         548  AGGTCCC              [GA]G^G[AT]CC[CT]
              724  GGGACCT             
PstI          499  CTGCAG               CTGCA^G
              787  CTGCAG              
RsaI          216  GTAC                 GT^AC
              481  GTAC                
SfaNI         442  GCATC                GCATC(5/9)
              519  GCATC               
SfcI          499  CTGCAG               C^T[GA][CT]AG
              787  CTGCAG              
Tsp45I        368  GTGAC                ^GT[GC]AC
              751  GTCAC               
Van91I        246  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              321  CCATGCCCTGG         
XhoII         331  GGATCC               [GA]^GATC[CT]
              805  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       Tru9I       
TseI        TspRI       
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