Restriction map for pF1KE6039
 (Linear) (Six-base) MAPSORT of: KIEE6039.seq  Check: 7328  from: 1  to: 951



KIEE6039.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     252     951 
   Size:        252     699
 
BamHI G'GATC_C

Cuts at:      0     331     951 
   Size:        331     620
 
BanI G'GyrC_C

Cuts at:      0     580     951 
   Size:        580     371
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     628     951 
   Size:        628     323
 
BfrBI ATG'CAT

Cuts at:      0     417     951 
   Size:        417     534
 
BlpI GC'TnA_GC

Cuts at:      0     599     951 
   Size:        599     352
 
Bme1580I G_kGCm'C

Cuts at:      0     583     951 
   Size:        583     368
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     903     951 
   Size:        903      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     660     951 
   Size:        660     291
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     648     951 
   Size:        648     303
 
BseYI C'CCAG_C

Cuts at:      0     245     951 
   Size:        245     706
 
BsiHKAI G_wGCw'C

Cuts at:      0      98     560     951 
   Size:         98     462     391

  Fragments arranged by size: 

                 462     391      98
 
BsmI GAATG_Cn'

Cuts at:      0     718     951 
   Size:        718     233
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     560     583     951 
   Size:         98     462      23     368

  Fragments arranged by size: 

                 462     368      98      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      28     736     951 
   Size:         28     708     215

  Fragments arranged by size: 

                 708     215      28
 
BsrDI GCAATG_nn'

Cuts at:      0     668     951 
   Size:        668     283
 
BstEII G'GTnAC_C

Cuts at:      0     750     849     951 
   Size:        750      99     102

  Fragments arranged by size: 

                 750     102      99
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147     477     951 
   Size:        147     330     474

  Fragments arranged by size: 

                 474     330     147
 
BstYI r'GATC_y

Cuts at:      0     331     805     951 
   Size:        331     474     146

  Fragments arranged by size: 

                 474     331     146
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     505     951 
   Size:        505     446
 
BtsI GCAGTG_nn'

Cuts at:      0     240     686     951 
   Size:        240     446     265

  Fragments arranged by size: 

                 446     265     240
 
DraIII CAC_nnn'GTG

Cuts at:      0     341     681     951 
   Size:        341     340     270

  Fragments arranged by size: 

                 341     340     270
 
EarI CTCTTCn'nnn_

Cuts at:      0     841     951 
   Size:        841     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     549     725     951 
   Size:        549     176     226

  Fragments arranged by size: 

                 549     226     176
 
HindIII A'AGCT_T

Cuts at:      0     169     951 
   Size:        169     782
 
MslI CAynn'nnrTG

Cuts at:      0      20     778     951 
   Size:         20     758     173

  Fragments arranged by size: 

                 758     173      20
 
MspA1I CmG'CkG

Cuts at:      0     221     504     951 
   Size:        221     283     447

  Fragments arranged by size: 

                 447     283     221
 
NdeI CA'TA_TG

Cuts at:      0     176     951 
   Size:        176     775
 
NsiI A_TGCA'T

Cuts at:      0     419     951 
   Size:        419     532
 
NspI r_CATG'y

Cuts at:      0     861     951 
   Size:        861      90
 
PasI CC'CwG_GG

Cuts at:      0     327     951 
   Size:        327     624
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     252     327     951 
   Size:        252      75     624

  Fragments arranged by size: 

                 624     252      75
 
PpuMI rG'GwC_Cy

Cuts at:      0     549     725     951 
   Size:        549     176     226

  Fragments arranged by size: 

                 549     226     176
 
PshAI GACnn'nnGTC

Cuts at:      0     748     951 
   Size:        748     203
 
PstI C_TGCA'G

Cuts at:      0     503     791     951 
   Size:        503     288     160

  Fragments arranged by size: 

                 503     288     160
 
PvuII CAG'CTG

Cuts at:      0     504     951 
   Size:        504     447
 
SfcI C'TryA_G

Cuts at:      0     499     787     951 
   Size:        499     288     164

  Fragments arranged by size: 

                 499     288     164
 
SmlI C'TyrA_G

Cuts at:      0     882     951 
   Size:        882      69
 
SphI G_CATG'C

Cuts at:      0     861     951 
   Size:        861      90
 
SspI AAT'ATT

Cuts at:      0     945     951 
   Size:        945       6
 
StyI C'CwwG_G

Cuts at:      0     437     951 
   Size:        437     514
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     144     951 
   Size:        144     807
 
XmnI GAAnn'nnTTC

Cuts at:      0     718     951 
   Size:        718     233
 

 Enzymes that do cut:

    AlwNI    BamHI     BanI    BciVI    BfrBI     BlpI Bme1580I    BpuEI
     BsaI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
   BstEII    BstXI    BstYI    BtgZI     BtsI   DraIII     EarI EcoO109I
  HindIII     MslI   MspA1I     NdeI     NsiI     NspI     PasI    PflMI
    PpuMI    PshAI     PstI    PvuII     SfcI     SmlI     SphI     SspI
     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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