Restriction map for pF1KE2237
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# NO CUT ENZYMES (  119 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvrII       
BalI        BbrPI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

AatII           6  GACGTC               GACGT^C
AcsI           61  AAATTT               [GA]^AATT[CT]
AocI         1556  CCTCAGG              CC^T[ACGT]AGG
ApoI           61  AAATTT               [GA]^AATT[CT]
AviII        1973  TGCGCA               TGC^GCA
BbeI         1234  GGCGCC               GGCGC^C
BbsI         1389  GTCTTC               GAAGAC(2/6)
BcgI          471  GCAGTTGTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          149  CCCGG                CC^[GC]GG
BglI         1614  GCCCAGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1389  GTCTTC               GAAGAC(2/6)
BsaBI         132  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          206  GGTCTC               GGTCTC(1/5)
BsmBI         106  GAGACG               CGTCTC(1/5)
BstEII       1445  GGTTACC              G^GT[ACGT]ACC
BstPI        1445  GGTTACC              G^GT[ACGT]ACC
Bsu36I       1556  CCTCAGG              CC^T[ACGT]AGG
DraIII       1172  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1556  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1445  GGTTACC              G^GT[ACGT]ACC
FspI         1973  TGCGCA               TGC^GCA
HaeII        1234  GGCGCC               [GA]GCGC^[CT]
HincII        220  GTTAAC               GT[CT]^[GA]AC
HindII        220  GTTAAC               GT[CT]^[GA]AC
HpaI          220  GTTAAC               GTT^AAC
KasI         1234  GGCGCC               G^GCGCC
MamI          132  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1234  GGCGCC               GG^CGCC
NciI          149  CCCGG                CC^[GC]GG
NspI         2006  ACATGC               [GA]CATG^[CT]
PpuMI        1336  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         722  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          189  GAGCTC               GAGCT^C
SfoI         1234  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccII          56  CGCG                 CG^CG
             1314  CGCG                
AlwNI        1345  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1931  CAGCAGCTG           
BamHI        1719  GGATCC               G^GATCC
             1785  GGATCC              
BanI          296  GGCACC               G^G[CT][GA]CC
             1234  GGCGCC              
BbvCI        1075  CCTCAGC              CCTCAGC(-5/-2)
             1293  CCTCAGC             
BciVI         555  GGATAC               GTATCC(6/5)
             1416  GGATAC              
BsrFI        1643  ACCGGC               [GA]^CCGG[CT]
             2069  ACCGGC              
BstUI          56  CGCG                 CG^CG
             1314  CGCG                
BstXI         863  CCACCCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1151  CCACCTCCCTGG        
Cfr10I       1643  ACCGGC               [GA]^CCGG[CT]
             2069  ACCGGC              
MaeII           7  ACGT                 A^CGT
              600  ACGT                
MvnI           56  CGCG                 CG^CG
             1314  CGCG                
PflMI         300  CCATGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1768  CCAATGGGTGG         
PvuII        1348  CAGCTG               CAG^CTG
             1934  CAGCTG              
TfiI          277  GATTC                G^A[AT]TC
             1671  GAATC               
Van91I        300  CCATGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1768  CCAATGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaII       BanII       BbvI        
BfaI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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