Restriction map for pF1KE2237
 (Linear) (Six-base) MAPSORT of: KIEE2237.seq  Check: 4666  from: 1  to: 2079



KIEE2237.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     807    2079 
   Size:        807    1272
 
AatII G_ACGT'C

Cuts at:      0      10    2079 
   Size:         10    2069
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700     899    1072    1757    2079 
   Size:        700     199     173     685     322

  Fragments arranged by size: 

                 700     685     322     199     173
 
AleI CACnn'nnGTG

Cuts at:      0    1283    2079 
   Size:       1283     796
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1350    1936    2079 
   Size:       1350     586     143

  Fragments arranged by size: 

                1350     586     143
 
ApaI G_GGCC'C

Cuts at:      0     979    1321    1616    2079 
   Size:        979     342     295     463

  Fragments arranged by size: 

                 979     463     342     295
 
ApoI r'AATT_y

Cuts at:      0      61    2079 
   Size:         61    2018
 
BamHI G'GATC_C

Cuts at:      0    1719    1785    2079 
   Size:       1719      66     294

  Fragments arranged by size: 

                1719     294      66
 
BanI G'GyrC_C

Cuts at:      0     296    1234    2079 
   Size:        296     938     845

  Fragments arranged by size: 

                 938     845     296
 
BanII G_rGCy'C

Cuts at:      0     193     259     820     979    1096    1321    1616    1699 
   Size:        193      66     561     159     117     225     295      83

Cuts at:   1699    2079 
   Size:        380

  Fragments arranged by size: 

                 561     380     295     225     193     159     117      83
                  66
 
BbeI G_GCGC'C

Cuts at:      0    1238    2079 
   Size:       1238     841
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1382    2079 
   Size:       1382     697
 
BbvCI CC'TCA_GC

Cuts at:      0    1076    1294    2079 
   Size:       1076     218     785

  Fragments arranged by size: 

                1076     785     218
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     460     494    2079 
   Size:        460      34    1585

  Fragments arranged by size: 

                1585     460      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     549    1410    2079 
   Size:        549     861     669

  Fragments arranged by size: 

                 861     669     549
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1620    2079 
   Size:       1620     459
 
Bme1580I G_kGCm'C

Cuts at:      0     979    1292    1321    1616    2028    2079 
   Size:        979     313      29     295     412      51

  Fragments arranged by size: 

                 979     412     313     295      51      29
 
BmgBI CAC'GTC

Cuts at:      0     601    2079 
   Size:        601    1478
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     539    1610    1996    2079 
   Size:        539    1071     386      83

  Fragments arranged by size: 

                1071     539     386      83
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1091    2079 
   Size:       1091     988
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1039    1696    1856    2079 
   Size:       1039     657     160     223

  Fragments arranged by size: 

                1039     657     223     160
 
Bpu10I CC'TnA_GC

Cuts at:      0    1039    1066    1076    1294    1836    2079 
   Size:       1039      27      10     218     542     243

  Fragments arranged by size: 

                1039     542     243     218      27      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     212    2079 
   Size:        212    1867
 
BsaBI GATnn'nnATC

Cuts at:      0     136    2079 
   Size:        136    1943
 
BsaHI Gr'CG_yC

Cuts at:      0       7    1235    1328    1406    2079 
   Size:          7    1228      93      78     673

  Fragments arranged by size: 

                1228     673      93      78       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     904     934    1206    1236    2079 
   Size:        127      30     747      30     272      30     843

  Fragments arranged by size: 

                 843     747     272     127      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     136     398     664    1113    1182    1185    1188 
   Size:        118      18     262     266     449      69       3       3

Cuts at:   1188    1197    1200    1857    2065    2079 
   Size:          9       3     657     208      14

  Fragments arranged by size: 

                 657     449     266     262     208     118      69      18
                  14       9       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     580     592    1082    1320    1490    2079 
   Size:        580      12     490     238     170     589

  Fragments arranged by size: 

                 589     580     490     238     170      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     476     826    1570    2079 
   Size:        340     136     350     744     509

  Fragments arranged by size: 

                 744     509     350     340     136
 
BsiHKAI G_wGCw'C

Cuts at:      0     193     292    1182    2079 
   Size:        193      99     890     897

  Fragments arranged by size: 

                 897     890     193      99
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     100    2079 
   Size:        100    1979
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     259     292     820     979    1096    1182    1292 
   Size:        193      66      33     528     159     117      86     110

Cuts at:   1292    1321    1616    1699    2028    2079 
   Size:         29     295      83     329      51

  Fragments arranged by size: 

                 528     329     295     193     159     117     110      86
                  83      66      51      33      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     648     807     848    2079 
   Size:        648     159      41    1231

  Fragments arranged by size: 

                1231     648     159      41
 
BsrFI r'CCGG_y

Cuts at:      0    1643    2069    2079 
   Size:       1643     426      10

  Fragments arranged by size: 

                1643     426      10
 
BstEII G'GTnAC_C

Cuts at:      0    1445    2079 
   Size:       1445     634
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     870    1158    2079 
   Size:        870     288     921

  Fragments arranged by size: 

                 921     870     288
 
BstYI r'GATC_y

Cuts at:      0     327    1719    1785    2079 
   Size:        327    1392      66     294

  Fragments arranged by size: 

                1392     327     294      66
 
Bsu36I CC'TnA_GG

Cuts at:      0    1557    2079 
   Size:       1557     522
 
BtgI C'CryG_G

Cuts at:      0      35     300     685    1830    2079 
   Size:         35     265     385    1145     249

  Fragments arranged by size: 

                1145     385     265     249      35
 
BtsI GCAGTG_nn'

Cuts at:      0     856    2070    2079 
   Size:        856    1214       9

  Fragments arranged by size: 

                1214     856       9
 
DraIII CAC_nnn'GTG

Cuts at:      0    1177    2079 
   Size:       1177     902
 
EaeI y'GGCC_r

Cuts at:      0      32     242    1645    1904    2079 
   Size:         32     210    1403     259     175

  Fragments arranged by size: 

                1403     259     210     175      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     720     875    1206    2079 
   Size:        720     155     331     873

  Fragments arranged by size: 

                 873     720     331     155
 
EcoICRI GAG'CTC

Cuts at:      0     191    2079 
   Size:        191    1888
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700     899    1039    1072    1696    1757    1856    2079 
   Size:        700     199     140      33     624      61      99     223

  Fragments arranged by size: 

                 700     624     223     199     140      99      61      33
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     568    1265    1337    1741    1840    2079 
   Size:        568     697      72     404      99     239

  Fragments arranged by size: 

                 697     568     404     239      99      72
 
FspI TGC'GCA

Cuts at:      0    1975    2079 
   Size:       1975     104
 
HaeII r_GCGC'y

Cuts at:      0    1238    2079 
   Size:       1238     841
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     127     159    1684    1716    2079 
   Size:        127      32    1525      32     363

  Fragments arranged by size: 

                1525     363     127      32      32
 
HincII GTy'rAC

Cuts at:      0     222    2079 
   Size:        222    1857
 
HpaI GTT'AAC

Cuts at:      0     222    2079 
   Size:        222    1857
 
KasI G'GCGC_C

Cuts at:      0    1234    2079 
   Size:       1234     845
 
MslI CAynn'nnrTG

Cuts at:      0     200     530    1283    2079 
   Size:        200     330     753     796

  Fragments arranged by size: 

                 796     753     330     200
 
MspA1I CmG'CkG

Cuts at:      0    1350    1443    1936    2079 
   Size:       1350      93     493     143

  Fragments arranged by size: 

                1350     493     143      93
 
NarI GG'CG_CC

Cuts at:      0    1235    2079 
   Size:       1235     844
 
NcoI C'CATG_G

Cuts at:      0     300     685    1830    2079 
   Size:        300     385    1145     249

  Fragments arranged by size: 

                1145     385     300     249
 
NspI r_CATG'y

Cuts at:      0    2010    2079 
   Size:       2010      69
 
PasI CC'CwG_GG

Cuts at:      0    1158    1261    2079 
   Size:       1158     103     818

  Fragments arranged by size: 

                1158     818     103
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     306    1774    2079 
   Size:        306    1468     305

  Fragments arranged by size: 

                1468     306     305
 
PpuMI rG'GwC_Cy

Cuts at:      0    1337    2079 
   Size:       1337     742
 
PshAI GACnn'nnGTC

Cuts at:      0     726    2079 
   Size:        726    1353
 
PspOMI G'GGCC_C

Cuts at:      0     975    1317    1612    2079 
   Size:        975     342     295     467

  Fragments arranged by size: 

                 975     467     342     295
 
PstI C_TGCA'G

Cuts at:      0      86     462    1031    2079 
   Size:         86     376     569    1048

  Fragments arranged by size: 

                1048     569     376      86
 
PvuII CAG'CTG

Cuts at:      0    1350    1936    2079 
   Size:       1350     586     143

  Fragments arranged by size: 

                1350     586     143
 
SacI G_AGCT'C

Cuts at:      0     193    2079 
   Size:        193    1886
 
SfcI C'TryA_G

Cuts at:      0      82     387     458    1027    1513    2079 
   Size:         82     305      71     569     486     566

  Fragments arranged by size: 

                 569     566     486     305      82      71
 
SfoI GGC'GCC

Cuts at:      0    1236    2079 
   Size:       1236     843
 
StyI C'CwwG_G

Cuts at:      0     300     685    1127    1830    2079 
   Size:        300     385     442     703     249

  Fragments arranged by size: 

                 703     442     385     300     249
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     716    1034    1395    1762    2079 
   Size:        716     318     361     367     317

  Fragments arranged by size: 

                 716     367     361     318     317
 
TatI w'GTAC_w

Cuts at:      0    1140    2079 
   Size:       1140     939
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1206    1238    2079 
   Size:       1206      32     841

  Fragments arranged by size: 

                1206     841      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     232     422     945    1155    1713    2079 
   Size:        232     190     523     210     558     366

  Fragments arranged by size: 

                 558     523     366     232     210     190
 
ZraI GAC'GTC

Cuts at:      0       8    2079 
   Size:          8    2071
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AleI    AlwNI     ApaI     ApoI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I   HincII     HpaI     KasI     MslI   MspA1I     NarI     NcoI
     NspI     PasI    PflMI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI     SfcI     SfoI     StyI    TaqII     TatI     TstI     XcmI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BclI    BfrBI    BglII     BlpI     BmtI    BpuEI    BsaAI
    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI
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