Restriction map for pF1KE2653
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AvaII       
AviII       AvrII       BamHI       BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        ItaI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MunI        MvnI        NaeI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TseI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI         1236  CCGC                 CCGC(-3/-1)
AcyI         1208  GGCGCC               G[GA]^CG[CT]C
AflIII        647  ACATGT               A^C[GA][CT]GT
AlwI         1498  GATCC                GGATC(4/5)
Aor51HI       918  AGCGCT               AGC^GCT
AspI         1186  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1208  GGCGCC               G^G[CT][GA]CC
BanII          41  GGGCTC               G[GA]GC[CT]^C
BbeI         1208  GGCGCC               GGCGC^C
BglII         259  AGATCT               A^GATCT
BmyI           41  GGGCTC               G[AGT]GC[ACT]^C
BsaHI        1208  GGCGCC               G[GA]^CG[CT]C
BsaI          168  GGTCTC               GGTCTC(1/5)
BseRI         968  GAGGAG               GAGGAG(10/8)
BsiYI         433  CCATATTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          433  CCATATTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I       41  GGGCTC               G[AGT]GC[ACT]^C
Bsp1407I     1004  TGTACA               T^GTACA
BspDI        1202  ATCGAT               AT^CGAT
BspLU11I      647  ACATGT               A^CATGT
BsrGI        1004  TGTACA               T^GTACA
BssSI        1052  CACGAG               CACGAG(-5/-1)
BstAPI       1510  GCAATTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I       1120  GGGCC                G^G[ACGT]CC
ClaI         1202  ATCGAT               AT^CGAT
Eco47III      918  AGCGCT               AGC^GCT
EcoRI         808  GAATTC               G^AATTC
EcoRV         484  GATATC               GAT^ATC
Hin1I        1208  GGCGCC               G[GA]^CG[CT]C
KasI         1208  GGCGCC               G^GCGCC
MaeII        1448  ACGT                 A^CGT
NarI         1208  GGCGCC               GG^CGCC
NdeI          346  CATATG               CA^TATG
NspI          647  ACATGT               [GA]CATG^[CT]
PflFI        1186  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         433  CCATATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I       1120  GGGCC                G^G[ACGT]CC
SfoI         1208  GGCGCC               GGC^GCC
SspBI        1004  TGTACA               T^GTACA
Tth111I      1186  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        433  CCATATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI          277  GAATTT               [GA]^AATT[CT]
              808  GAATTC              
AfaI          383  GTAC                 GT^AC
             1005  GTAC                
AlwNI         381  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              699  CAGTAACTG           
ApoI          277  GAATTT               [GA]^AATT[CT]
              808  GAATTC              
BalI         1075  TGGCCA               TGG^CCA
             1251  TGGCCA              
BbsI          536  GTCTTC               GAAGAC(2/6)
             1373  GTCTTC              
BcnI          711  CCCGG                CC^[GC]GG
              798  CCGGG               
BfaI          296  CTAG                 C^TAG
              998  CTAG                
BpuAI         536  GTCTTC               GAAGAC(2/6)
             1373  GTCTTC              
BsgI         1428  CTGCAC               GTGCAG(16/14)
              368  GTGCAG              
BsmAI         659  GAGAC                GTCTC(1/5)
              169  GTCTC               
BsmFI         416  GGGAC                GGGAC(10/14)
              430  GTCCC               
BsrDI        1181  GCAATG               GCAATG(2/0)
             1238  GCAATG              
BstYI         259  AGATCT               [GA]^GATC[CT]
             1497  AGATCC              
EcoT14I       146  CCAAGG               C^C[AT][AT]GG
              214  CCTTGG              
HapII         712  CCGG                 C^CGG
              798  CCGG                
HinfI        1319  GATTC                G^A[ACGT]TC
             1522  GAATC               
HpaII         712  CCGG                 C^CGG
              798  CCGG                
MaeI          296  CTAG                 C^TAG
              998  CTAG                
MflI          259  AGATCT               [GA]^GATC[CT]
             1497  AGATCC              
MluNI        1075  TGGCCA               TGG^CCA
             1251  TGGCCA              
MscI         1075  TGGCCA               TGG^CCA
             1251  TGGCCA              
MslI          346  CATATGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1036  CATCCTTATG          
MspI          712  CCGG                 C^CGG
              798  CCGG                
NciI          711  CCCGG                CC^[GC]GG
              798  CCGGG               
RsaI          383  GTAC                 GT^AC
             1005  GTAC                
StyI          146  CCAAGG               C^C[AT][AT]GG
              214  CCTTGG              
TfiI         1319  GATTC                G^A[AT]TC
             1522  GAATC               
TspRI         551  CACTG                CA[GC]TG[ACGT][ACGT]^
              777  CAGTG               
XcmI          433  CCATATTCTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1290  CCAAGTGGCTTTTGG     
XhoII         259  AGATCT               [GA]^GATC[CT]
             1497  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        AseI        AsnI        BpmI        BsaJI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        DdeI        DpnII       EaeI        EcoRII      
FokI        HaeII       HaeIII      HhaI        HinP1I      
HphI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PshBI       Sau3AI      ScrFI       SfaNI       
TaqI        Tru9I       Tsp45I      Tsp509I     TthHB8I     
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