Restriction map for pF1KE2788
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaBI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HhaI        HinP1I      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MamI        MboI        
MflI        MluI        MroI        MslI        MspI        
MvnI        NaeI        NarI        NciI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          745  GTAGAC               GT^[AC][GT]AC
AcsI          105  AAATTC               [GA]^AATT[CT]
AcyI          720  GGCGTC               G[GA]^CG[CT]C
AlwNI         712  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          105  AAATTC               [GA]^AATT[CT]
AseI          409  ATTAAT               AT^TAAT
AsnI          409  ATTAAT               AT^TAAT
BalI          404  TGGCCA               TGG^CCA
BbsI          758  GTCTTC               GAAGAC(2/6)
BpuAI         758  GTCTTC               GAAGAC(2/6)
BsaAI         163  TACGTG               [CT]AC^GT[GA]
BsaHI         720  GGCGTC               G[GA]^CG[CT]C
BsaJI         491  CCATGG               C^C[ACGT][ACGT]GG
BseRI          97  GAGGAG               GAGGAG(10/8)
BsmI          204  GCATTC               GAATGC(1/-1)
BspMI          75  GCAGGT               ACCTGC(4/8)
BsrDI         890  GCAATG               GCAATG(2/0)
DsaI          491  CCATGG               C^C[GA][CT]GG
EaeI          404  TGGCCA               [CT]^GGCC[GA]
EcoT14I       491  CCATGG               C^C[AT][AT]GG
HgaI          721  GCGTC                GACGC(5/10)
Hin1I         720  GGCGTC               G[GA]^CG[CT]C
HincII        837  GTCAAC               GT[CT]^[GA]AC
HindII        837  GTCAAC               GT[CT]^[GA]AC
HphI          399  GGTGA                GGTGA(8/7)
MaeII         164  ACGT                 A^CGT
MfeI          829  CAATTG               C^AATTG
MluNI         404  TGGCCA               TGG^CCA
MscI          404  TGGCCA               TGG^CCA
MseI          410  TTAA                 T^TAA
MspA1I        544  CAGCGG               C[AC]G^C[GT]G
MunI          829  CAATTG               C^AATTG
MwoI          218  GCCAACATAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          491  CCATGG               C^CATGG
PleI          239  GAGTC                GAGTC(4/5)
PshBI         409  ATTAAT               AT^TAAT
ScaI           32  AGTACT               AGT^ACT
SmlI           28  CTTGAG               C^T[CT][GA]AG
StyI          491  CCATGG               C^C[AT][AT]GG
Tru9I         410  TTAA                 T^TAA
XbaI          769  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          819  CCGC                 CCGC(-3/-1)
              546  GCGG                
AluI          169  AGCT                 AG^CT
              592  AGCT                
BfaI          770  CTAG                 C^TAG
              866  CTAG                
BsaI          325  GAGACC               GGTCTC(1/5)
              787  GAGACC              
BspHI         693  TCATGA               T^CATGA
              734  TCATGA              
BssKI         396  CCAGG                ^CC[ACGT]GG
              639  CCTGG               
BstNI         396  CCAGG                CC^[AT]GG
              639  CCTGG               
Cac8I         316  GCCAGC               GC[ACGT]^[ACGT]GC
              593  GCTTGC              
Cfr13I        393  GGGCC                G^G[ACGT]CC
              457  GGCCC               
EarI          195  CTCTTC               CTCTTC(1/4)
               94  GAAGAG              
EcoRII        396  CCAGG                ^CC[AT]GG
              639  CCTGG               
Ksp632I       195  CTCTTC               CTCTTC(1/4)
               94  GAAGAG              
MaeI          770  CTAG                 C^TAG
              866  CTAG                
MvaI          396  CCAGG                CC^[AT]GG
              639  CCTGG               
NlaIV         336  GGTTCC               GG[ACGT]^[ACGT]CC
              392  GGGGCC              
PflMI         396  CCAGGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              711  CCAGATTCTGG         
PstI          250  CTGCAG               CTGCA^G
              293  CTGCAG              
RcaI          693  TCATGA               T^CATGA
              734  TCATGA              
Sau96I        393  GGGCC                G^G[ACGT]CC
              457  GGCCC               
ScrFI         396  CCAGG                CC^[ACGT]GG
              639  CCTGG               
SfaNI         313  GATGC                GCATC(5/9)
              575  GCATC               
SfcI          250  CTGCAG               C^T[GA][CT]AG
              293  CTGCAG              
Van91I        396  CCAGGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              711  CCAGATTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AfaI        BbvI        BpmI        BsiYI       BslI        
BsmAI       BsrI        BstF5I      DdeI        Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MaeIII      
MboII       MnlI        NlaIII      RsaI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       
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