Restriction map for pF1KE2788
 (Circular) (Six-base) MAPSORT of: pF1KE2788.seq  Check: 6015  from: 1  to: 4016



pF1KE2788  4016 bp

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    174     174 
   Size:       4016
 
AccI GT'mk_AC

Cuts at:    854    1054     854 
   Size:        200    3816
 
Acc65I G'GTAC_C

Cuts at:   1032    1032 
   Size:       4016
 
AclI AA'CG_TT

Cuts at:   3856    3856 
   Size:       4016
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    290     595    1841    2273    3020    3288     290 
   Size:        305    1246     432     747     268    1018

  Fragments arranged by size: 

                1246    1018     747     432     305     268
 
AflIII A'CryG_T

Cuts at:   1368    2472    3449    1368 
   Size:       1104     977    1935

  Fragments arranged by size: 

                1935    1104     977
 
AgeI A'CCGG_T

Cuts at:   2404    2404 
   Size:       4016
 
AlwNI CAG_nnn'CTG

Cuts at:    825    1321    2888     825 
   Size:        496    1567    1953

  Fragments arranged by size: 

                1953    1567     496
 
ApaLI G'TGCA_C

Cuts at:   2786    2786 
   Size:       4016
 
ApoI r'AATT_y

Cuts at:    213    1020    3369    3625     213 
   Size:        807    2349     256     604

  Fragments arranged by size: 

                2349     807     604     256
 
AseI AT'TA_AT

Cuts at:     20     518      20 
   Size:        498    3518
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4016
 
AvaI C'yCGr_G

Cuts at:   1036    1036 
   Size:       4016
 
BamHI G'GATC_C

Cuts at:   1041    1041 
   Size:       4016
 
BanI G'GyrC_C

Cuts at:   1032    1446    1694    1729    1032 
   Size:        414     248      35    3319

  Fragments arranged by size: 

                3319     414     248      35
 
BanII G_rGCy'C

Cuts at:   1030    2060    1030 
   Size:       1030    2986
 
BbeI G_GCGC'C

Cuts at:   1450    1698    1450 
   Size:        248    3768
 
BbsI GAAGACnn'nnnn_

Cuts at:    859     859 
   Size:       4016
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3982
 
BciVI GTATCCnnnnn_n'

Cuts at:   1914    2675    4016    1914 
   Size:        761    1341    1914

  Fragments arranged by size: 

                1914    1341     761
 
BclI T'GATC_A

Cuts at:   1536    1536 
   Size:       4016
 
BglII A'GATC_T

Cuts at:   1531    3568    1531 
   Size:       2037    1979
 
BlpI GC'TnA_GC

Cuts at:   1128    1128 
   Size:       4016
 
Bme1580I G_kGCm'C

Cuts at:   1641    1734    2790    1641 
   Size:         93    1056    2867

  Fragments arranged by size: 

                2867    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    620    1228    1309    1644    3788     620 
   Size:        608      81     335    2144     848

  Fragments arranged by size: 

                2144     848     608     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2383    2383 
   Size:       4016
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    180     769     913     180 
   Size:        589     144    3283

  Fragments arranged by size: 

                3283     589     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    157    1192    2071    2563    2861     157 
   Size:       1035     879     492     298    1312

  Fragments arranged by size: 

                1312    1035     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    427     889    3720     427 
   Size:        462    2831     723

  Fragments arranged by size: 

                2831     723     462
 
BsaAI yAC'GTr

Cuts at:    273    1369     273 
   Size:       1096    2920
 
BsaBI GATnn'nnATC

Cuts at:      6    1530    3567       6 
   Size:       1524    2037     455

  Fragments arranged by size: 

                2037    1524     455
 
BsaHI Gr'CG_yC

Cuts at:    829    1447    1695    2397    3893     829 
   Size:        618     248     702    1496     952

  Fragments arranged by size: 

                1496     952     702     618     248
 
BsaWI w'CCGG_w

Cuts at:   1205    1726    2404    2678    2825    3236    1205 
   Size:        521     678     274     147     411    1985

  Fragments arranged by size: 

                1985     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    220     220 
   Size:       4016
 
BseYI C'CCAG_C

Cuts at:    190    1441    2199    2776    3272     190 
   Size:       1251     758     577     496     934

  Fragments arranged by size: 

                1251     934     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1558    1604    2812     104 
   Size:       1454      46    1208    1308

  Fragments arranged by size: 

                1454    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1030    1808    1998    2790    1030 
   Size:        778     190     792    2256

  Fragments arranged by size: 

                2256     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    312     312 
   Size:       4016
 
Bsp1286I G_dGCh'C

Cuts at:   1030    1641    1734    1808    1998    2060    2790    1030 
   Size:        611      93      74     190      62     730    2256

  Fragments arranged by size: 

                2256     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3236    3236 
   Size:       4016
 
BspHI T'CATG_A

Cuts at:    801     842    3192     801 
   Size:         41    2350    1625

  Fragments arranged by size: 

                2350    1625      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    174    1066    1280    1582    1963     174 
   Size:        892     214     302     381    2227

  Fragments arranged by size: 

                2227     892     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2308    2362    3846    4011      50 
   Size:       2258      54    1484     165      55

  Fragments arranged by size: 

                2258    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1005    1928    1005 
   Size:        923    3093
 
BsrFI r'CCGG_y

Cuts at:   2014    2195    2404    2014 
   Size:        181     209    3626

  Fragments arranged by size: 

                3626     209     181
 
BssSI C'ACGA_G

Cuts at:   2287    2645    2287 
   Size:        358    3658
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1393    1393 
   Size:       4016
 
BstBI TT'CG_AA

Cuts at:   2377    2377 
   Size:       4016
 
BstYI r'GATC_y

Cuts at:      1    1041    1508    1531    1866    2112    3124    3210    3222 
   Size:       1040     467      23     335     246    1012      86      12

Cuts at:   3222    3568       1 
   Size:        346     449

  Fragments arranged by size: 

                1040    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    599     599 
   Size:       4016
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1966    2147    2310    1966 
   Size:        181     163    3672

  Fragments arranged by size: 

                3672     181     163
 
BtsI GCAGTG_nn'

Cuts at:    737    1259     737 
   Size:        522    3494
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    145     180     145 
   Size:         35    3981
 
DraI TTT'AAA

Cuts at:   1015    1015 
   Size:       4016
 
DrdI GACnn_nn'nnGTC

Cuts at:   1722    2580    1722 
   Size:        858    3158
 
EaeI y'GGCC_r

Cuts at:    512    1601    1775    2166    2193    3538     512 
   Size:       1089     174     391      27    1345     990

  Fragments arranged by size: 

                1345    1089     990     391     174      27
 
EagI C'GGCC_G

Cuts at:   1601    1601 
   Size:       4016
 
EarI CTCTTCn'nnn_

Cuts at:    197     309    2039    2249     197 
   Size:        112    1730     210    1964

  Fragments arranged by size: 

                1964    1730     210     112
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2224    2534    2680    3826    2224 
   Size:        310     146    1146    2414

  Fragments arranged by size: 

                2414    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1028    1028 
   Size:       4016
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    180     290     595     769     913    1841    2273    3020    3288 
   Size:        110     305     174     144     928     432     747     268

Cuts at:   3288     180 
   Size:        908

  Fragments arranged by size: 

                 928     908     747     432     305     268     174     144
                 110
 
EcoO109I rG'GnC_Cy

Cuts at:   1155    1155 
   Size:       4016
 
EcoRI G'AATT_C

Cuts at:   1020    1020 
   Size:       4016
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2035    2035 
   Size:       4016
 
FspI TGC'GCA

Cuts at:   1797    1797 
   Size:       4016
 
HaeII r_GCGC'y

Cuts at:   1450    1698    2720    3699    1450 
   Size:        248    1022     979    1767

  Fragments arranged by size: 

                1767    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    526     558     902     934    1858    1888    1890    1920     526 
   Size:         32     344      32     924      30       2      30    2622

  Fragments arranged by size: 

                2622     924     344      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    947    1055     947 
   Size:        108    3908
 
KasI G'GCGC_C

Cuts at:   1446    1694    1446 
   Size:        248    3768
 
KpnI G_GTAC'C

Cuts at:   1036    1036 
   Size:       4016
 
MfeI C'AATT_G

Cuts at:    937     937 
   Size:       4016
 
MluI A'CGCG_T

Cuts at:   3449    3449 
   Size:       4016
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2687    2871    2687 
   Size:        184    3832
 
MscI TGG'CCA

Cuts at:    514    1777     514 
   Size:       1263    2753
 
MslI CAynn'nnrTG

Cuts at:   2005    2132    2005 
   Size:        127    3889
 
MspA1I CmG'CkG

Cuts at:    654    1127    1441    1801    2814    3059     654 
   Size:        473     314     360    1013     245    1611

  Fragments arranged by size: 

                1611    1013     473     360     314     245
 
NaeI GCC'GGC

Cuts at:   2197    2197 
   Size:       4016
 
NarI GG'CG_CC

Cuts at:   1447    1695    1447 
   Size:        248    3768
 
NcoI C'CATG_G

Cuts at:    599     599 
   Size:       4016
 
NgoMIV G'CCGG_C

Cuts at:   2195    2195 
   Size:       4016
 
NspI r_CATG'y

Cuts at:   1069    1569    2476    1069 
   Size:        500     907    2609

  Fragments arranged by size: 

                2609     907     500
 
PciI A'CATG_T

Cuts at:   2472    2472 
   Size:       4016
 
PflMI CCAn_nnn'nTGG

Cuts at:    510     825     510 
   Size:        315    3701
 
PmeI GTTT'AAAC

Cuts at:   1015    1015 
   Size:       4016
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3155    3187    3155 
   Size:         32    3984
 
PsiI TTA'TAA

Cuts at:    176     479     176 
   Size:        303    3713
 
PstI C_TGCA'G

Cuts at:    362     405    1063    1748     362 
   Size:         43     658     685    2630

  Fragments arranged by size: 

                2630     685     658      43
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4016
 
PvuII CAG'CTG

Cuts at:   1441    1801    1441 
   Size:        360    3656
 
RsrII CG'GwC_CG

Cuts at:   2211    2211 
   Size:       4016
 
SacI G_AGCT'C

Cuts at:   1030    1030 
   Size:       4016
 
SalI G'TCGA_C

Cuts at:   1053    1053 
   Size:       4016
 
SapI GCTCTTCn'nnn_

Cuts at:   2039    2249    2039 
   Size:        210    3806
 
SbfI CC_TGCA'GG

Cuts at:   1063    1063 
   Size:       4016
 
ScaI AGT'ACT

Cuts at:    142    3533     142 
   Size:       3391     625
 
SfcI C'TryA_G

Cuts at:     33     358     401    1059    1744    2737    2928      33 
   Size:        325      43     658     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     658     325     191      43
 
SfoI GGC'GCC

Cuts at:   1448    1696    1448 
   Size:        248    3768
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4016
 
SmaI CCC'GGG

Cuts at:   1038    1038 
   Size:       4016
 
SmlI C'TyrA_G

Cuts at:    136    1171    2086    2578    2840     136 
   Size:       1035     915     492     262    1312

  Fragments arranged by size: 

                1312    1035     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4016
 
SphI G_CATG'C

Cuts at:   1069    1569    1069 
   Size:        500    3516
 
StyI C'CwwG_G

Cuts at:    599    1150    1504     599 
   Size:        551     354    3111

  Fragments arranged by size: 

                3111     551     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1599    1732    2191    2400    1599 
   Size:        133     459     209    3215

  Fragments arranged by size: 

                3215     459     209     133
 
TatI w'GTAC_w

Cuts at:    140     779    3531     140 
   Size:        639    2752     625

  Fragments arranged by size: 

                2752     639     625
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3533    3565    3533 
   Size:         32    3984
 
Tth111I GACn'n_nGTC

Cuts at:   1813    1813 
   Size:       4016
 
XbaI T'CTAG_A

Cuts at:    877    1047     877 
   Size:        170    3846
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1511    1511 
   Size:       4016
 
XmaI C'CCGG_G

Cuts at:   1036    1036 
   Size:       4016
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PmeI     PpiI     PsiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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