Restriction map for pF1KE3275
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsiWI       BsmI        Bsp1407I    
BspDI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraII       DrdI        
Eam1105I    Eco47III    EcoO109I    EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HaeII       
Hin1I       HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PvuI        PvuII       RsrII       
SacII       SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I       1054  GGTACC               G^GTACC
AccII          79  CGCG                 CG^CG
AccIII        116  TCCGGA               T^CCGGA
AclI          535  AACGTT               AA^CGTT
AflIII        390  ACACGT               A^C[GA][CT]GT
AocI          241  CCTCAGG              CC^T[ACGT]AGG
AseI          226  ATTAAT               AT^TAAT
AsnI          226  ATTAAT               AT^TAAT
Asp718I      1054  GGTACC               G^GTACC
AvaII           6  GGACC                G^G[AT]CC
BanI         1054  GGTACC               G^G[CT][GA]CC
BbsI          295  GAAGAC               GAAGAC(2/6)
BcgI           86  GCAAGAGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          515  CTAG                 C^TAG
BglII        1002  AGATCT               A^GATCT
BpmI           10  CTGGAG               CTGGAG(16/14)
BpuAI         295  GAAGAC               GAAGAC(2/6)
BsaAI         391  CACGTA               [CT]AC^GT[GA]
BsaWI         116  TCCGGA               [AT]^CCGG[AT]
BseAI         116  TCCGGA               T^CCGGA
BsgI         1921  GTGCAG               GTGCAG(16/14)
BsiEI          71  CGGCCG               CG[GA][CT]^CG
BsmBI        1292  GAGACG               CGTCTC(1/5)
BspEI         116  TCCGGA               T^CCGGA
BspHI         338  TCATGA               T^CATGA
BstAPI       1582  GCAGTAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          79  CGCG                 CG^CG
Bsu36I        241  CCTCAGG              CC^T[ACGT]AGG
CfoI           80  GCGC                 GCG^C
DraI          202  TTTAAA               TTT^AAA
DraIII        856  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           71  CGGCCG               C^GGCCG
EclXI          71  CGGCCG               C^GGCCG
Eco52I         71  CGGCCG               C^GGCCG
Eco81I        241  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      1854  ATGCAT               ATGCA^T
FauI           77  CCCGC                CCCGC(4/6)
HhaI           80  GCGC                 GCG^C
HinP1I         80  GCGC                 G^CGC
KpnI         1054  GGTACC               GGTAC^C
MaeI          515  CTAG                 C^TAG
MroI          116  TCCGGA               T^CCGGA
MslI         1042  CATGCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1963  CAGCGG               C[AC]G^C[GT]G
MvnI           79  CGCG                 CG^CG
NsiI         1854  ATGCAT               ATGCA^T
PshBI         226  ATTAAT               AT^TAAT
Psp1406I      535  AACGTT               AA^CGTT
PstI         1084  CTGCAG               CTGCA^G
RcaI          338  TCATGA               T^CATGA
SacI         2020  GAGCTC               GAGCT^C
SalI          314  GTCGAC               G^TCGAC
SapI           54  GAAGAGC              GCTCTTC(1/4)
SmaI         1418  CCCGGG               CCC^GGG
SphI         1136  GCATGC               GCATG^C
SspI          964  AATATT               AAT^ATT
XmaCI        1418  CCCGGG               C^CCGGG
XmaI         1418  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI         632  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2009  CAGGTACTG           
AspHI        1345  GAGCAC               G[AT]GC[AT]^C
             2020  GAGCTC              
AvaI         1075  CCCGAG               C^[CT]CG[GA]G
             1418  CCCGGG              
BalI           44  TGGCCA               TGG^CCA
             1641  TGGCCA              
BanII         106  GAGCCC               G[GA]GC[CT]^C
             2020  GAGCTC              
BciVI        1473  GGATAC               GTATCC(6/5)
              113  GTATCC              
BseRI         596  GAGGAG               GAGGAG(10/8)
             1772  GAGGAG              
BsiHKAI      1345  GAGCAC               G[AT]GC[AT]^C
             2020  GAGCTC              
BsoBI        1075  CCCGAG               C^[CT]CG[GA]G
             1418  CCCGGG              
BsrDI         652  GCAATG               GCAATG(2/0)
             1896  GCAATG              
BstYI         813  GGATCT               [GA]^GATC[CT]
             1002  AGATCT              
Cfr13I          6  GGACC                G^G[ACGT]CC
             1959  GGCCC               
DsaI           47  CCATGG               C^C[GA][CT]GG
             1059  CCATGG              
EcoNI        1269  CCTTTTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2002  CCTCGGTCAGG         
EcoT14I        47  CCATGG               C^C[AT][AT]GG
             1059  CCATGG              
HgaI         1731  GCGTC                GACGC(5/10)
             1974  GCGTC               
HincII        314  GTCGAC               GT[CT]^[GA]AC
             1366  GTTGAC              
HindII        314  GTCGAC               GT[CT]^[GA]AC
             1366  GTTGAC              
MflI          813  GGATCT               [GA]^GATC[CT]
             1002  AGATCT              
MluNI          44  TGGCCA               TGG^CCA
             1641  TGGCCA              
MscI           44  TGGCCA               TGG^CCA
             1641  TGGCCA              
NcoI           47  CCATGG               C^CATGG
             1059  CCATGG              
PleI         1719  GACTC                GAGTC(4/5)
             1783  GAGTC               
Sau96I          6  GGACC                G^G[ACGT]CC
             1959  GGCCC               
StyI           47  CCATGG               C^C[AT][AT]GG
             1059  CCATGG              
XhoII         813  GGATCT               [GA]^GATC[CT]
             1002  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       DdeI        DpnII       EaeI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
RsaI        Sau3AI      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XmnI        
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