Restriction map for pF1KE3275
 (Linear) (Six-base) MAPSORT of: KIEE3275.seq  Check: 4742  from: 1  to: 2064



KIEE3275.orf

 With 182 enzymes: SgfI * 

                        July 27, 2017 10:26  ..

AccI GT'mk_AC

Cuts at:      0     315     941    1382    2064 
   Size:        315     626     441     682

  Fragments arranged by size: 

                 682     626     441     315
 
Acc65I G'GTAC_C

Cuts at:      0    1054    2064 
   Size:       1054    1010
 
AclI AA'CG_TT

Cuts at:      0     536    2064 
   Size:        536    1528
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1270    1282    2064 
   Size:       1270      12     782

  Fragments arranged by size: 

                1270     782      12
 
AflIII A'CryG_T

Cuts at:      0     390    2064 
   Size:        390    1674
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     675    2064 
   Size:        675    1389
 
AlwNI CAG_nnn'CTG

Cuts at:      0     637    2014    2064 
   Size:        637    1377      50

  Fragments arranged by size: 

                1377     637      50
 
ApoI r'AATT_y

Cuts at:      0     320     455     490    1794    2064 
   Size:        320     135      35    1304     270

  Fragments arranged by size: 

                1304     320     270     135      35
 
AseI AT'TA_AT

Cuts at:      0     227    2064 
   Size:        227    1837
 
AvaI C'yCGr_G

Cuts at:      0    1075    1418    2064 
   Size:       1075     343     646

  Fragments arranged by size: 

                1075     646     343
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1378    1411    2064 
   Size:       1378      33     653

  Fragments arranged by size: 

                1378     653      33
 
BanI G'GyrC_C

Cuts at:      0    1054    2064 
   Size:       1054    1010
 
BanII G_rGCy'C

Cuts at:      0     110    2024    2064 
   Size:        110    1914      40

  Fragments arranged by size: 

                1914     110      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     302    2064 
   Size:        302    1762
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      75     109    2064 
   Size:         75      34    1955

  Fragments arranged by size: 

                1955      75      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     124    1467    2064 
   Size:        124    1343     597

  Fragments arranged by size: 

                1343     597     124
 
BfrBI ATG'CAT

Cuts at:      0    1856    2064 
   Size:       1856     208
 
BglII A'GATC_T

Cuts at:      0    1002    2064 
   Size:       1002    1062
 
Bme1580I G_kGCm'C

Cuts at:      0    1983    2064 
   Size:       1983      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     104    1711    2064 
   Size:        104    1607     353

  Fragments arranged by size: 

                1607     353     104
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    2064 
   Size:         31    2033
 
BsaAI yAC'GTr

Cuts at:      0     393    2064 
   Size:        393    1671
 
BsaWI w'CCGG_w

Cuts at:      0     116    2064 
   Size:        116    1948
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     868     898    1108    1138    1236    1266    2064 
   Size:        868      30     210      30      98      30     798

  Fragments arranged by size: 

                 868     798     210      98      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     611    1787    2064 
   Size:        611    1176     277

  Fragments arranged by size: 

                1176     611     277
 
BseYI C'CCAG_C

Cuts at:      0    1308    1961    2064 
   Size:       1308     653     103

  Fragments arranged by size: 

                1308     653     103
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1942    2064 
   Size:       1942     122
 
BsiEI CG_ry'CG

Cuts at:      0      74    2064 
   Size:         74    1990
 
BsiHKAI G_wGCw'C

Cuts at:      0    1349    2024    2064 
   Size:       1349     675      40

  Fragments arranged by size: 

                1349     675      40
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1286    2064 
   Size:       1286     778
 
Bsp1286I G_dGCh'C

Cuts at:      0     110    1349    1983    2024    2064 
   Size:        110    1239     634      41      40

  Fragments arranged by size: 

                1239     634     110      41      40
 
BspEI T'CCGG_A

Cuts at:      0     116    2064 
   Size:        116    1948
 
BspHI T'CATG_A

Cuts at:      0     338    2064 
   Size:        338    1726
 
BsrDI GCAATG_nn'

Cuts at:      0     659    1903    2064 
   Size:        659    1244     161

  Fragments arranged by size: 

                1244     659     161
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1588    2064 
   Size:       1588     476
 
BstYI r'GATC_y

Cuts at:      0     813    1002    2064 
   Size:        813     189    1062

  Fragments arranged by size: 

                1062     813     189
 
Bsu36I CC'TnA_GG

Cuts at:      0     242    2064 
   Size:        242    1822
 
BtgI C'CryG_G

Cuts at:      0      47    1059    2064 
   Size:         47    1012    1005

  Fragments arranged by size: 

                1012    1005      47
 
BtsI GCAGTG_nn'

Cuts at:      0    1116    1930    2064 
   Size:       1116     814     134

  Fragments arranged by size: 

                1116     814     134
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1388    1423    2064 
   Size:       1388      35     641

  Fragments arranged by size: 

                1388     641      35
 
DraI TTT'AAA

Cuts at:      0     204    2064 
   Size:        204    1860
 
DraIII CAC_nnn'GTG

Cuts at:      0     861    2064 
   Size:        861    1203
 
EaeI y'GGCC_r

Cuts at:      0      23      44      71    1641    2064 
   Size:         23      21      27    1570     423

  Fragments arranged by size: 

                1570     423      27      23      21
 
EagI C'GGCC_G

Cuts at:      0      71    2064 
   Size:         71    1993
 
EarI CTCTTCn'nnn_

Cuts at:      0      49     254     667    1463    1612    2064 
   Size:         49     205     413     796     149     452

  Fragments arranged by size: 

                 796     452     413     205     149      49
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    2064 
   Size:         19    2045
 
EcoICRI GAG'CTC

Cuts at:      0    2022    2064 
   Size:       2022      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    1270    1282    2064 
   Size:         31    1239      12     782

  Fragments arranged by size: 

                1239     782      31      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1273    2006    2064 
   Size:       1273     733      58

  Fragments arranged by size: 

                1273     733      58
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     319    2064 
   Size:        319    1745
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     162     194     414     446    1108    1140    1234    1266 
   Size:        162      32     220      32     662      32      94      32

Cuts at:   1266    1441    1473    1768    1800    2064 
   Size:        175      32     295      32     264

  Fragments arranged by size: 

                 662     295     264     220     175     162      94      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     316    1368    2064 
   Size:        316    1052     696

  Fragments arranged by size: 

                1052     696     316
 
KpnI G_GTAC'C

Cuts at:      0    1058    2064 
   Size:       1058    1006
 
MscI TGG'CCA

Cuts at:      0      46    1643    2064 
   Size:         46    1597     421

  Fragments arranged by size: 

                1597     421      46
 
MslI CAynn'nnrTG

Cuts at:      0    1046    2064 
   Size:       1046    1018
 
MspA1I CmG'CkG

Cuts at:      0    1965    2064 
   Size:       1965      99
 
NcoI C'CATG_G

Cuts at:      0      47    1059    2064 
   Size:         47    1012    1005

  Fragments arranged by size: 

                1012    1005      47
 
NsiI A_TGCA'T

Cuts at:      0    1858    2064 
   Size:       1858     206
 
NspI r_CATG'y

Cuts at:      0     924    1140    1381    1695    2064 
   Size:        924     216     241     314     369

  Fragments arranged by size: 

                 924     369     314     241     216
 
PasI CC'CwG_GG

Cuts at:      0    1128    2064 
   Size:       1128     936
 
PfoI T'CCnGG_A

Cuts at:      0     809    2064 
   Size:        809    1255
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1802    1834    2064 
   Size:       1802      32     230

  Fragments arranged by size: 

                1802     230      32
 
PstI C_TGCA'G

Cuts at:      0    1088    2064 
   Size:       1088     976
 
SacI G_AGCT'C

Cuts at:      0    2024    2064 
   Size:       2024      40
 
SalI G'TCGA_C

Cuts at:      0     314    2064 
   Size:        314    1750
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49    2064 
   Size:         49    2015
 
SfcI C'TryA_G

Cuts at:      0     383     942    1084    2064 
   Size:        383     559     142     980

  Fragments arranged by size: 

                 980     559     383     142
 
SmaI CCC'GGG

Cuts at:      0    1420    2064 
   Size:       1420     644
 
SphI G_CATG'C

Cuts at:      0    1140    2064 
   Size:       1140     924
 
SspI AAT'ATT

Cuts at:      0     966    2064 
   Size:        966    1098
 
StyI C'CwwG_G

Cuts at:      0      47    1059    2064 
   Size:         47    1012    1005

  Fragments arranged by size: 

                1012    1005      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1223    1994    2064 
   Size:       1223     771      70

  Fragments arranged by size: 

                1223     771      70
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1236    1268    2064 
   Size:       1236      32     796

  Fragments arranged by size: 

                1236     796      32
 
XmaI C'CCGG_G

Cuts at:      0    1418    2064 
   Size:       1418     646
 
XmnI GAAnn'nnTTC

Cuts at:      0     467     568     902    1814    2064 
   Size:        467     101     334     912     250

  Fragments arranged by size: 

                 912     467     334     250     101
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AlfI    AlwNI     ApoI
     AseI     AvaI     BaeI     BanI    BanII     BbsI     BcgI    BciVI
    BfrBI    BglII Bme1580I     BmrI     BpmI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BsrDI   BstAPI    BstYI   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI     FalI    Hin4I   HincII     KpnI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI     PasI     PfoI     PpiI     PstI     SacI
     SalI     SapI     SfcI     SmaI     SphI     SspI     StyI    TaqII
     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI
     BclI     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsiWI     BsmI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI     DrdI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI
    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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