Restriction map for pF1KE2370
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# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp509I     TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII         485  CGCG                 CG^CG
ApaI           43  GGGCCC               GGGCC^C
Asp700I       231  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII          43  GGGCCC               G[GA]GC[CT]^C
BbvI          108  GCTGC                GCAGC(8/12)
BmyI           43  GGGCCC               G[AGT]GC[ACT]^C
BseRI         490  GAGGAG               GAGGAG(10/8)
BsgI          265  GTGCAG               GTGCAG(16/14)
BsiEI         198  CGACCG               CG[GA][CT]^CG
BsiYI         479  CCGTGTCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          479  CCGTGTCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I       43  GGGCCC               G[AGT]GC[ACT]^C
BspMI         104  ACCTGC               ACCTGC(4/8)
BsrFI          59  GCCGGC               [GA]^CCGG[CT]
BstUI         485  CGCG                 CG^CG
Cfr10I         59  GCCGGC               [GA]^CCGG[CT]
DdeI          185  CTGAG                C^T[ACGT]AG
DrdI          205  GACAAGTTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          154  CTCTTC               CTCTTC(1/4)
FauI          486  GCGGG                CCCGC(4/6)
HaeII         305  AGCGCC               [GA]GCGC^[CT]
HinfI         172  GACTC                G^A[ACGT]TC
HphI          254  TCACC                GGTGA(8/7)
Ksp632I       154  CTCTTC               CTCTTC(1/4)
MaeIII          7  GTGAC                ^GT[ACGT]AC
MslI          474  CATCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          485  CGCG                 CG^CG
NaeI           59  GCCGGC               GCC^GGC
NgoMIV         59  GCCGGC               G^CCGGC
NlaIII        411  CATG                 CATG^
NspI          410  GCATGC               [GA]CATG^[CT]
PleI          172  GACTC                GAGTC(4/5)
PpuMI         248  AGGACCT              [GA]G^G[AT]CC[CT]
SphI          410  GCATGC               GCATG^C
TseI          108  GCTGC                G^C[AT]GC
Tsp45I          7  GTGAC                ^GT[GC]AC
XmnI          231  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaII         249  GGACC                G^G[AT]CC
              372  GGACC               
BcnI          201  CCGGG                CC^[GC]GG
              243  CCCGG               
BsmFI         203  GGGAC                GGGAC(10/14)
              150  GTCCC               
BstYI         284  AGATCC               [GA]^GATC[CT]
              434  AGATCC              
CfoI           33  GCGC                 GCG^C
              306  GCGC                
DsaI          288  CCACGG               C^C[GA][CT]GG
              318  CCACGG              
Fnu4HI        108  GCTGC                GC^[ACGT]GC
              347  GCCGC               
HhaI           33  GCGC                 GCG^C
              306  GCGC                
HinP1I         33  GCGC                 G^CGC
              306  GCGC                
ItaI          108  GCTGC                GC^[ACGT]GC
              347  GCCGC               
MaeII           5  ACGT                 A^CGT
              368  ACGT                
MboII         155  TCTTC                GAAGA(8/7)
              218  TCTTC               
MflI          284  AGATCC               [GA]^GATC[CT]
              434  AGATCC              
NciI          201  CCGGG                CC^[GC]GG
              243  CCCGG               
SfaNI         451  GATGC                GCATC(5/9)
              179  GCATC               
XhoII         284  AGATCC               [GA]^GATC[CT]
              434  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AlwI        BsaJI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DpnII       
DraII       EcoO109I    EcoRII      FokI        HaeIII      
HapII       HpaII       MboI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIV       Sau3AI      
Sau96I      ScrFI       TaqI        TthHB8I     
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